Title:
EPITOPE NORMALIZED ANTIBODY PANEL, METHOD FOR CREATING SAME AND UTILIZATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2018/092907
Kind Code:
A1
Abstract:
Provided is technology for efficiently screening antibodies or technology for searching for and specifying a functional region of an antigen by an antibody. More specifically, provided is an epitope normalized antibody panel. Also provided is a method for creating an epitope normalized antibody panel against a specific antigen, said method comprising: (a) a step for providing a population of antibodies against the antigen as an original antibody panel; (b) a step for acquiring binding data of the antibodies in the original antibody panel; (c) a step for clustering the original antibody panel on the basis of the binding data; (d) a step for forming a partial panel, if required, and clustering the same; and (e) a step for computing the epitope group number e of the panel and, when a panel satisfying e≥E [wherein E stands for the target epitope group number relating to the antigen] exists, regarding the panel as an epitope normalized antibody panel, and when no such panel exists, creating a new population of antibodies by adding new antibodies and then repeating the aforesaid steps (a) to (d).
Inventors:
KONDO HIROSATO (JP)
KAMADA HARUHIKO (JP)
NAGATA SATOSHI (JP)
MIZUGUCHI KENJI (JP)
MURAKAMI YOICHI (JP)
TSUMOTO KOUHEI (JP)
AKIBA HIROKI (JP)
KAMADA HARUHIKO (JP)
NAGATA SATOSHI (JP)
MIZUGUCHI KENJI (JP)
MURAKAMI YOICHI (JP)
TSUMOTO KOUHEI (JP)
AKIBA HIROKI (JP)
Application Number:
PCT/JP2017/041683
Publication Date:
May 24, 2018
Filing Date:
November 20, 2017
Export Citation:
Assignee:
NAT INST BIOMEDICAL INNOVATION HEALTH & NUTRITION (JP)
International Classes:
C07K16/00; C12N15/09; C12P21/08; G01N33/53
Foreign References:
| JP2016509009A | 2016-03-24 | |||
| JP2005512043A | 2005-04-28 |
Other References:
SIVASUBRAMANIAN, A. ET AL.: "Broad epitope coverage of a human in vitro antibody library", MABS, vol. 9, no. 1, 17 October 2016 (2016-10-17), pages 29 - 42, XP055509595
ABDICHE, Y. N. ET AL.: "High-throughput epitope binning assays on label-free array-based biosensors can yield exquisite epitope discrimination that facilitates the selection of monoclonal antibodies with functional activity", PLOS ONE, vol. 9, no. 3, 1 March 2014 (2014-03-01), pages e92451, XP055509756
ABDICHE, Y. N. ET AL.: "High-throughput epitope binning assays on label-free array-based biosensors can yield exquisite epitope discrimination that facilitates the selection of monoclonal antibodies with functional activity", PLOS ONE, vol. 9, no. 3, 1 March 2014 (2014-03-01), pages e92451, XP055509756
Attorney, Agent or Firm:
YAMAMOTO Shusaku et al. (JP)
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