Title:
ESCHERICHIA COLI MEMBRANE PROTEIN INSERTION FACTOR
Document Type and Number:
WIPO Patent Application WO/2011/108687
Kind Code:
A1
Abstract:
Disclosed are: a novel Escherichia coli membrane protein insertion factor which is involved in the intake of a protein; and a method for utilizing the Escherichia coli membrane protein insertion factor particularly in the synthesis of a membrane protein in a cell-free system and the insertion of a protein into a membrane which occurs in conjunction with the aforementioned synthesis. Specifically disclosed is a membrane protein insertion factor in a highly purified form, which is characterized by having a structure represented by chemical formula (Ia) or (II). (In the formulae, R1 represents a saturated /unsaturated fatty acid residue that has a carbon chain containing 16 to 20 carbon atoms; R2 represents a hydrogen atom or an acetyl group; n represents an integer of 20 or less; GlcNAc represents N-acetylglucosamine; ManNAcA represents N-acetylmannosamine uronic acid; and Fuc4NAc represents 4-N-acetylaminofucose.)
Inventors:
KUSUMOTO SHOICHI (JP)
SHIMAMOTO KEIKO (JP)
MAEDA MASAHIDE (JP)
NISHIYAMA KENICHI (JP)
TOKUDA HAJIME (JP)
UEDA TAKUYA (JP)
KANAMORI TAKASHI (JP)
SHIMAMOTO KEIKO (JP)
MAEDA MASAHIDE (JP)
NISHIYAMA KENICHI (JP)
TOKUDA HAJIME (JP)
UEDA TAKUYA (JP)
KANAMORI TAKASHI (JP)
Application Number:
PCT/JP2011/055026
Publication Date:
September 09, 2011
Filing Date:
March 04, 2011
Export Citation:
Assignee:
SUNTORY FOUNDATION FOR LIFE SCIENCES (JP)
KUSUMOTO SHOICHI (JP)
SHIMAMOTO KEIKO (JP)
MAEDA MASAHIDE (JP)
NISHIYAMA KENICHI (JP)
TOKUDA HAJIME (JP)
UEDA TAKUYA (JP)
KANAMORI TAKASHI (JP)
KUSUMOTO SHOICHI (JP)
SHIMAMOTO KEIKO (JP)
MAEDA MASAHIDE (JP)
NISHIYAMA KENICHI (JP)
TOKUDA HAJIME (JP)
UEDA TAKUYA (JP)
KANAMORI TAKASHI (JP)
International Classes:
C12P19/26; C07H13/06; C08B37/00; C12N15/09; C12P21/02
Other References:
NISHIYAMA, K. ET AL.: "A derivative of lipid A is involved in signal recognition particle/ SecYEG-dependent and -independent membrane integrations", J.BIOL.CHEM., vol. 281, no. 47, 2006, pages 35667 - 35676
KEN'ICHI NISHIYAMA: "Molecular mechanisms underlying membrane protein integrations in E. coli", JOURNAL OF JAPANESE BIOCHEMICAL SOCIETY, vol. 79, no. 3, 2007, pages 254 - 262
NISHIYAMA, K. ET AL.: "A novel complete reconstitution system for membrane integration of the simplest membrane protein", BIOCHEM. BIOPHYS.RES.COMMUN., vol. 394, no. 3, April 2010 (2010-04-01), pages 733 - 736
KEN'ICHI NISHIYAMA: "Molecular mechanisms underlying membrane protein integrations in E. coli", JOURNAL OF JAPANESE BIOCHEMICAL SOCIETY, vol. 79, no. 3, 2007, pages 254 - 262
NISHIYAMA, K. ET AL.: "A novel complete reconstitution system for membrane integration of the simplest membrane protein", BIOCHEM. BIOPHYS.RES.COMMUN., vol. 394, no. 3, April 2010 (2010-04-01), pages 733 - 736
Attorney, Agent or Firm:
KUSAMA, OSAMU (JP)
Osamu Kusama (JP)
Osamu Kusama (JP)
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Claims: