Title:
ESTERASE MUTANT AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/004969
Kind Code:
A1
Abstract:
Provided are an esterase mutant and the use thereof. The esterase mutant obtained by means of rational design and several rounds of evolution screening with enzymes on the basis of an amino acid sequence as shown in SEQ ID NO: 1 is changed in terms of protein structure and functions compared with a wild-type esterase; in practical use, the catalytic activity and/or stereoselectivity of the esterase mutant is greatly improved; and when a system contains some organic cosolvents, the esterase mutant still has relatively stable catalytic activity and/or stereoselectivity. In addition, the improvement of the catalytic activity and/or stereoselectivity of the esterase mutant reduces the use amount of the enzyme to a certain extent and reduces the difficulty of post-treatment, and therefore the esterase mutant is suitable for industrial production.
More Like This:
| JP2005506044 | Biosynthesis of polyketide synthase substrate |
| WO/1986/005812 | SECRETION VECTOR |
| WO2001055336 | METHODS AND MATERIALS RELATING TO CD84-LIKE POLYPEPTIDES AND POLYNUCLEOTIDES |
Inventors:
HONG HAO (US)
JAMES GAGE (US)
XIAO YI (CN)
ZHANG NA (CN)
JIAO XUECHENG (CN)
YANG YIMING (CN)
LI JUAN (CN)
HUANG QIXING (CN)
JAMES GAGE (US)
XIAO YI (CN)
ZHANG NA (CN)
JIAO XUECHENG (CN)
YANG YIMING (CN)
LI JUAN (CN)
HUANG QIXING (CN)
Application Number:
PCT/CN2021/119423
Publication Date:
February 02, 2023
Filing Date:
September 18, 2021
Export Citation:
Assignee:
ASYMCHEM LAB TIANJIN CO LTD (CN)
International Classes:
C12N9/16; C12N1/21; C12N15/55; C12N15/70; C12P7/02; C12P7/40; C12R1/19
Foreign References:
| CN113308450A | 2021-08-27 | |||
| CN101001954A | 2007-07-18 | |||
| CN112430585A | 2021-03-02 | |||
| CN102573451A | 2012-07-11 | |||
| CN112375751A | 2021-02-19 | |||
| CN107058362A | 2017-08-18 | |||
| CN106929493A | 2017-07-07 |
Attorney, Agent or Firm:
KANGXIN PARTNERS, P.C. (CN)
Download PDF: