Title:
EVALUATION METHOD OF ANTICANCER AGENT AND KIT FOR EVALUATING ANTICANCER AGENT
Document Type and Number:
WIPO Patent Application WO/2024/101303
Kind Code:
A1
Abstract:
An evaluation method of an anticancer agent that includes the following steps in this order: a step of culturing a cell structure containing stromal cells, in which a gene encoding a viable cell marker protein has been knocked out, and cancer cells in the presence of the anticancer agent; and a step of evaluating the anticancer agent using the expression level of the viable cell marker protein as an indicator. In the aforesaid evaluation, the expression level of the viable cell marker protein is measured using the culture supernatant. Alternatively, in the aforesaid evaluation, a substance that fluoresces or colors upon reaction with the viable cell marker protein is added and the expression level of the viable cell marker protein is measured on the basis of the amount of fluorescence or coloration of the substance.
Inventors:
TSUKAMOTO KEI (JP)
KITANO SHIRO (JP)
KITANO SHIRO (JP)
Application Number:
PCT/JP2023/039861
Publication Date:
May 16, 2024
Filing Date:
November 06, 2023
Export Citation:
Assignee:
TOPPAN HOLDINGS INC (JP)
International Classes:
C12Q1/06; C12N5/09; C12N5/10; C12N15/09; C12Q1/37
Domestic Patent References:
| WO2019039452A1 | 2019-02-28 | |||
| WO2017183673A1 | 2017-10-26 |
Foreign References:
| US20140026234A1 | 2014-01-23 |
Other References:
山添 泰宗, Fabrication of novel culture system composed of cancer cells and cancer stromal cells for in vitro evaluation of anticancer drugs, 科学研究費助成事業 研究成果報告書, 2014, (YAMAZOE, Hironori. Grants-in Aid for Scientific Research, Final research report.) Outline of research results, 3(1), 4(1)
TOFANI, L. B. et al. Generation of a three-dimensional in vitro ovarian cancer co-culture model for drug screening assays. Journal of Pharmaceutical Sciences. 2021, vol. 110, pp. 2629-2636,
TOFANI, L. B. et al. Generation of a three-dimensional in vitro ovarian cancer co-culture model for drug screening assays. Journal of Pharmaceutical Sciences. 2021, vol. 110, pp. 2629-2636,
Attorney, Agent or Firm:
MATSUNUMA Yasushi et al. (JP)
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