Title:
EVOLVED PROTEIN DEGRONS
Document Type and Number:
WIPO Patent Application WO/2023/245005
Kind Code:
A3
Abstract:
Aspects of the disclosure relate to compositions and methods for targeted protein degradation. In some embodiments, the disclosure relates to methods of evolving protein degrons to interact with certain small molecule inducers (e.g., VS-777, PT- 179, or PK-1016). In some embodiments, the disclosure relates to compositions (e.g., peptides, nucleic acids encoding the protein degrons, etc.) used for targeted protein degradation. In some embodiments, the disclosure relates to methods of degrading a target polypeptide in a cell.
Inventors:
LIU DAVID (US)
CHOUDHARY AMIT (US)
MERCER JARON (US)
DECARLO STEPHAN (US)
CHOUDHARY AMIT (US)
MERCER JARON (US)
DECARLO STEPHAN (US)
Application Number:
PCT/US2023/068349
Publication Date:
February 08, 2024
Filing Date:
June 13, 2023
Export Citation:
Assignee:
BROAD INST INC (US)
HARVARD COLLEGE (US)
BRIGHAM & WOMENS HOSPITAL INC (US)
HARVARD COLLEGE (US)
BRIGHAM & WOMENS HOSPITAL INC (US)
International Classes:
C07K14/47; C12N9/10
Domestic Patent References:
| WO2021188286A2 | 2021-09-23 | |||
| WO2022109421A1 | 2022-05-27 | |||
| WO2019089592A1 | 2019-05-09 | |||
| WO2021080427A1 | 2021-04-29 |
Other References:
JAN MAX ET AL: "Reversible ON- and OFF-switch chimeric antigen receptors controlled by lenalidomide", SCIENCE TRANSLATIONAL MEDICINE, vol. 13, no. 575, 6 January 2021 (2021-01-06), XP093093039, ISSN: 1946-6234, DOI: 10.1126/scitranslmed.abb6295
MILLER SHANNON M ET AL: "Phage-assisted continuous and non-continuous evolution", NATURE PROTOCOLS, vol. 15, no. 12, 16 November 2020 (2020-11-16), pages 4101 - 4127, XP037305621, ISSN: 1754-2189, DOI: 10.1038/S41596-020-00410-3
ESVELT KEVIN M ET AL: "A system for the continuous directed evolution of biomolecules", CLEO: APPLICATIONS AND TECHNOLOGY 2019 SAN JOSE, CALIFORNIA UNITED STATES 5-10 MAY 2019, OPTICA, vol. 472, no. 7344, 10 April 2011 (2011-04-10), pages 499 - 503, XP037291841, DOI: 10.1038/NATURE09929
XIE VICTORIA COCHRAN ET AL: "Methods for the directed evolution of biomolecular interactions", TRENDS IN BIOCHEMICAL SCIENCES, ELSEVIER, AMSTERDAM, NL, vol. 47, no. 5, 14 April 2022 (2022-04-14), pages 403 - 416, XP087019154, ISSN: 0968-0004, [retrieved on 20220414], DOI: 10.1016/J.TIBS.2022.01.001
MILLER SHANNON M ET AL: "Phage-assisted continuous and non-continuous evolution", NATURE PROTOCOLS, vol. 15, no. 12, 16 November 2020 (2020-11-16), pages 4101 - 4127, XP037305621, ISSN: 1754-2189, DOI: 10.1038/S41596-020-00410-3
ESVELT KEVIN M ET AL: "A system for the continuous directed evolution of biomolecules", CLEO: APPLICATIONS AND TECHNOLOGY 2019 SAN JOSE, CALIFORNIA UNITED STATES 5-10 MAY 2019, OPTICA, vol. 472, no. 7344, 10 April 2011 (2011-04-10), pages 499 - 503, XP037291841, DOI: 10.1038/NATURE09929
XIE VICTORIA COCHRAN ET AL: "Methods for the directed evolution of biomolecular interactions", TRENDS IN BIOCHEMICAL SCIENCES, ELSEVIER, AMSTERDAM, NL, vol. 47, no. 5, 14 April 2022 (2022-04-14), pages 403 - 416, XP087019154, ISSN: 0968-0004, [retrieved on 20220414], DOI: 10.1016/J.TIBS.2022.01.001
Attorney, Agent or Firm:
DAWSON, Cara, B. et al. (US)
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