Title:
EXOSOME PRODUCTION PROMOTING AGENT AND EXOSOME PRODUCTION PROMOTING METHOD
Document Type and Number:
WIPO Patent Application WO/2022/259526
Kind Code:
A1
Abstract:
Provided is an exosome production promoting agent that promotes the production of an exosome from adipose-derived mesenchymal stem cells. This exosome production promoting agent promotes the production of an exosome from adipose-derived mesenchymal stem cells cultured in a serum-free medium, and is characterized by comprising EGF. Further, including IL-1β and trehalose is preferable. It is desirable that SCGF is not included. Culturing with a non-woven fabric sheet as a scaffold is preferable. Exosomes produced in mass according to this technique can be used for the purpose of cosmetic surgery and the like.
Inventors:
CHIBA SHUNMEI (JP)
Application Number:
PCT/JP2021/022314
Publication Date:
December 15, 2022
Filing Date:
June 11, 2021
Export Citation:
Assignee:
FULLSTEM CO LTD (JP)
International Classes:
C12N5/0775; A61K35/12
Domestic Patent References:
| WO2018182356A1 | 2018-10-04 | |||
| WO2019088656A1 | 2019-05-09 | |||
| WO2019168000A1 | 2019-09-06 |
Foreign References:
| US20190133922A1 | 2019-05-09 | |||
| JP2019156786A | 2019-09-19 |
Other References:
TOMOHIRO KATO;SHIGERU MIYAKI;HIROYUKI ISHITOBI;YOSHIHIRO NAKAMURA;TOMOYUKI NAKASA;MARTIN K LOTZ;MITSUO OCHI: "Exosomes from IL-1beta stimulated synovial fibroblasts induce osteoarthritic changes in articular chondrocytes", ARTHRITIS RESEARCH AND THERAPY, BIOMED CENTRAL, LONDON, GB, vol. 16, no. 4, 4 August 2014 (2014-08-04), GB , pages R163, XP021193643, ISSN: 1478-6354, DOI: 10.1186/ar4679
ANONYMOUS: "The key to mass culture of stem cells was "non-woven fabric"! Regenerative Medicine Venture "Full Stem" Challenged by Brain Surgeons (ICC FUKUOKA 2020) [Transcript]", ICC SUMMIT FUKUOKA 2023, 21 June 2020 (2020-06-21), XP093017627, Retrieved from the Internet [retrieved on 20230125]
CHAMBERLAIN GFOX JASHTON BMIDDLETON J: "Concise review: mesenchymal stem cells: their phenotype, differentiation capacity, immunological features, and potential for homing", STEM CELLS, vol. 25, 2007, pages 2739 - 2749, XP055018344, DOI: 10.1634/stemcells.2007-0197
SIMONSRAPOSO, CURROPIN CELL BIOLOGY, vol. 21, 2009, pages 575 - 581
SKOG ET AL., NAT CELL BIOLOGY, vol. 10, 2008, pages 1470 - 1476
VALADI ET AL., NAT CELL BIOLOGY, vol. 9, 2007, pages 654 - 659
KATSUDA TKOSAKA NTAKESHITA FOCHIYA T: "The therapeutic potential of mesenchymal stem cell-derived extracellular vesicles", PROTEOMICS, vol. 13, 2013, pages 1637 - 1653, XP055732579, DOI: 10.1002/pmic.201200373
ANONYMOUS: "The key to mass culture of stem cells was "non-woven fabric"! Regenerative Medicine Venture "Full Stem" Challenged by Brain Surgeons (ICC FUKUOKA 2020) [Transcript]", ICC SUMMIT FUKUOKA 2023, 21 June 2020 (2020-06-21), XP093017627, Retrieved from the Internet
CHAMBERLAIN GFOX JASHTON BMIDDLETON J: "Concise review: mesenchymal stem cells: their phenotype, differentiation capacity, immunological features, and potential for homing", STEM CELLS, vol. 25, 2007, pages 2739 - 2749, XP055018344, DOI: 10.1634/stemcells.2007-0197
SIMONSRAPOSO, CURROPIN CELL BIOLOGY, vol. 21, 2009, pages 575 - 581
SKOG ET AL., NAT CELL BIOLOGY, vol. 10, 2008, pages 1470 - 1476
VALADI ET AL., NAT CELL BIOLOGY, vol. 9, 2007, pages 654 - 659
KATSUDA TKOSAKA NTAKESHITA FOCHIYA T: "The therapeutic potential of mesenchymal stem cell-derived extracellular vesicles", PROTEOMICS, vol. 13, 2013, pages 1637 - 1653, XP055732579, DOI: 10.1002/pmic.201200373
Attorney, Agent or Firm:
MAEDA & PARTNERS (JP)
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