Title:
EXPRESSION OF HUMAN PAPILLOMAVIRUS (HPV) 68 L1 PROTEIN, AND VIRUS-LIKE PARTICLES AND PREPARATION METHOD THEREFOR
Document Type and Number:
WIPO Patent Application WO/2024/088087
Kind Code:
A1
Abstract:
The present invention relates to the field of medical biology, and specifically to expression of a human papillomavirus (HPV) 68 L1 protein, and virus-like particles (VLPs) and a preparation method therefor. The amino acid sequence of the HPV 68 L1 protein of a specific virus strain, especially AAZ39498.1, is truncated, codon optimization is carried out on the coding nucleotide sequence of the truncated protein to obtain an optimized coding nucleotide sequence, and finally tag-free expression and purification are achieved by using a tag-free expression vector containing a specific SD sequence. According to the present invention, by means of the improvement, a higher protein expression level can be obtained in a prokaryotic expression system such as an E. coli expression system, and VLPs having more uniform quality are obtained.
Inventors:
ZHANG HAIJIANG (CN)
CHEN XIAO (CN)
WU SHUMING (CN)
LIU YONGJIANG (CN)
WANG XUEHONG (CN)
XUE JUNLIAN (CN)
GAO WENSHUANG (CN)
JIANG XULIN (CN)
SHEN ERCUI (CN)
YIN FEI (CN)
LIU YUYING (CN)
YU HONGYANG (CN)
CHEN XIAO (CN)
WU SHUMING (CN)
LIU YONGJIANG (CN)
WANG XUEHONG (CN)
XUE JUNLIAN (CN)
GAO WENSHUANG (CN)
JIANG XULIN (CN)
SHEN ERCUI (CN)
YIN FEI (CN)
LIU YUYING (CN)
YU HONGYANG (CN)
Application Number:
PCT/CN2023/124619
Publication Date:
May 02, 2024
Filing Date:
October 13, 2023
Export Citation:
Assignee:
BEIJING HEALTH GUARD BIOTECHNOLOGY INC (CN)
International Classes:
C07K14/025; C07K1/14; C07K1/18; C07K1/22; C12N1/21; C12N15/37; C12N15/70; C12R1/19
Attorney, Agent or Firm:
BEIJING ZHIWENTONGDA INTELLECTUAL PROPERTY AGENCY (CN)
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