Title:
EXPRESSION OF SARS-COV PROTEINS, NUCLEIC ACID CONSTRUCTS, VIRUS LIKE PROTEINS (VLPS) AND METHODS RELEVANT THERETO
Document Type and Number:
WIPO Patent Application WO/2021/176397
Kind Code:
A3
Abstract:
The present invention relates to expression of SARS-CoV like virus proteins [S, M and E] proteins; recombinant polynucleotides, polypeptides; constructs, virus-like particles (VLPs); immunogenic compositions or vaccines comprising Virus Like Particles (VLPs). Method of producing the VLPs/ expressing the multi-subunit virus like proteins and method for co-expression of multi-subunit and virus like proteins (VLPs) are also provided. The present invention also provides strategies, methods, systems, kits and combinations for scalable expression, purification and enhanced production of the virus like proteins of SARS-CoV while maintaining their size range and composition. Such multi-subunit VLPs can be utilized to make immunogenic compositions or vaccines.
Inventors:
ARORA KAJAL (IN)
RASTOGI RUCHIR (IN)
MEHROTRA NUPUR (IN)
KUNDU PRABUDDHA (IN)
RASTOGI RUCHIR (IN)
MEHROTRA NUPUR (IN)
KUNDU PRABUDDHA (IN)
Application Number:
PCT/IB2021/051828
Publication Date:
October 21, 2021
Filing Date:
March 04, 2021
Export Citation:
Assignee:
PREMAS BIOTECH PVT LTD (IN)
International Classes:
C12Q1/70; C07H21/04; C07K14/165; C12N15/86
Foreign References:
| US20060257852A1 | 2006-11-16 |
Other References:
CHEN ET AL.: "Yeast-expressed recombinant protein of the receptor-binding domain in SARS- CoV spike protein with deglycosylated forms as a SARS vaccine candidate", HUMAN VACCINES & IMMUNOTHERAPEUTICS, vol. 10, no. 3, 2014, pages 648 - 658, XP055824794, DOI: 10.4161/hv.27464
MACAULEY-PATRICK ET AL.: "Heterologous protein production using the Pichia pastoris expression system", YEAST, vol. 22, no. 4, 2005, pages 249 - 70, XP002474659, DOI: 10.1002/yea.1208
MACAULEY-PATRICK ET AL.: "Heterologous protein production using the Pichia pastoris expression system", YEAST, vol. 22, no. 4, 2005, pages 249 - 70, XP002474659, DOI: 10.1002/yea.1208
Attorney, Agent or Firm:
PANDEY, Shachindra Kumar et al. (IN)
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