Title:
EXPRESSION VECTOR AND METHOD FOR PRODUCING PROTEIN
Document Type and Number:
WIPO Patent Application WO/2013/111754
Kind Code:
A1
Abstract:
Provided are: an expression vector for secreting a protein (Z) to be produced or a fusion protein having the protein (Z) moiety therein; a method for producing a transformant using the expression vector; the transformant; and a method for producing a protein using the transformant.
An expression vector characterized by comprising an expression cassette that comprises a structural gene sequence (y) encoding a protein (Y) as mentioned below, a structural gene sequence (z) located downstream from the structural gene sequence (y) and encoding a protein (Z) that is a protein to be produced, and a promoter sequence and a terminator sequence both for expressing a fusion protein containing the protein (Y) moiety and the protein (Z) moiety, wherein the protein (Y) is a full-length protein of protein disulfide isomerase 1 (PDI1), a partial protein of PDI1, or a mutant protein of the full-length protein or the partial protein.
More Like This:
Inventors:
KOTANI TETSUYA (JP)
YIDIREI ALIMUJIANG (JP)
TOHDA HIDEKI (JP)
YIDIREI ALIMUJIANG (JP)
TOHDA HIDEKI (JP)
Application Number:
PCT/JP2013/051209
Publication Date:
August 01, 2013
Filing Date:
January 22, 2013
Export Citation:
Assignee:
ASAHI GLASS CO LTD (JP)
International Classes:
C12N15/09; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12P21/02
Domestic Patent References:
| WO1996023890A1 | 1996-08-08 | |||
| WO1999023223A1 | 1999-05-14 | |||
| WO2007026617A1 | 2007-03-08 | |||
| WO2007063919A1 | 2007-06-07 | |||
| WO2002101038A1 | 2002-12-19 | |||
| WO2007015470A1 | 2007-02-08 |
Foreign References:
| JPH1175879A | 1999-03-23 | |||
| JPS515380A | 1976-01-17 | |||
| JPH07163373A | 1995-06-27 | |||
| JPH10234375A | 1998-09-08 | |||
| JP2005198612A | 2005-07-28 |
Other References:
KAJINO, T. ET AL.: "A protein disulfide isomerase gene fusion expression system that increases the extracellular productivity of Bacillus brevis.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 66, no. 2, 2000, pages 638 - 642, XP002230940
TIAN, G. ET AL.: "The crystal structure of yeast protein disulfide isomerase suggests cooperativity between its active sites", CELL, vol. 124, 2006, pages 61 - 73, XP055156816
See also references of EP 2808388A4
MUKAIYAMA ET AL., APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 86, no. 4, 2010, pages 1135 - 1143
CELL, vol. 124, 2006, pages 61 - 73
J. SAMBROOK ET AL.: "Molecular Cloning", 1989, COLD SPRING HARBOR LABORATORY PRESS
NUCLEIC ACIDS RES., vol. 34, 2006, pages E11
"Koubo Bunshi Idengaku Jikken-Hou", 1996, JAPAN SCIENTIFIC SOCIETIES PRESS
PCR METHODS APPL., vol. 2, 1992, pages 28 - 33
YEAST, vol. 14, 1998, pages 943 - 951
CELL, vol. 45, 1986, pages 781 - 782
NATURE, vol. 318, 1985, pages 78 - 80
THE JOURNAL OF CELL BIOLOGY, vol. 109, 1989, pages 2693 - 2702
TIAN, G. ET AL.: "The crystal structure of yeast protein disulfide isomerase suggests cooperativity between its active sites", CELL, vol. 124, 2006, pages 61 - 73, XP055156816
See also references of EP 2808388A4
MUKAIYAMA ET AL., APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 86, no. 4, 2010, pages 1135 - 1143
CELL, vol. 124, 2006, pages 61 - 73
J. SAMBROOK ET AL.: "Molecular Cloning", 1989, COLD SPRING HARBOR LABORATORY PRESS
NUCLEIC ACIDS RES., vol. 34, 2006, pages E11
"Koubo Bunshi Idengaku Jikken-Hou", 1996, JAPAN SCIENTIFIC SOCIETIES PRESS
PCR METHODS APPL., vol. 2, 1992, pages 28 - 33
YEAST, vol. 14, 1998, pages 943 - 951
CELL, vol. 45, 1986, pages 781 - 782
NATURE, vol. 318, 1985, pages 78 - 80
THE JOURNAL OF CELL BIOLOGY, vol. 109, 1989, pages 2693 - 2702
Attorney, Agent or Firm:
SENMYO, Kenji et al. (JP)
Spring name Kenji (JP)
Spring name Kenji (JP)
Download PDF:
Claims: