Title:
EXTRACELLULAR VESICLE ISOLATION METHOD USING METAL
Document Type and Number:
WIPO Patent Application WO/2018/131966
Kind Code:
A1
Abstract:
The present invention relates to an extracellular vesicle isolation method using a metal, and more particularly, relates to a method for isolating extracellular vesicles from various samples using metal affinity. An extracellular vesicle isolation method according to the present invention has the advantages of not requiring costly equipment, of being able to be applied without limits on sample quantity, and of being capable of efficiently isolating extracellular vesicles while preserving the shape or properties thereof. Moreover, the method of the present invention can be combined with existing isolation methods to maximize the efficiency of extracellular vesicle isolation, and can be utilized in disease diagnosis, disease treatment, multiomics research, and extracellular vesicle property research and the like using the isolated extracellular vesicles.
Inventors:
GHO YONG SONG (KR)
LEE CHANG JIN (KR)
PARK HYUN TAEK (KR)
LEE CHANG JIN (KR)
PARK HYUN TAEK (KR)
Application Number:
PCT/KR2018/000673
Publication Date:
July 19, 2018
Filing Date:
January 15, 2018
Export Citation:
Assignee:
ROSETTA EXOSOME (KR)
POSTECH ACAD IND FOUND (KR)
POSTECH ACAD IND FOUND (KR)
International Classes:
C12N5/074; B01D15/38; C12N1/16; C12N1/20
Other References:
WAKO: "MagCaptureTM Exosome Isolation Kit PS", WAKO CATALOG, 20 January 2016 (2016-01-20), XP055519267, Retrieved from the Internet
ANONYMOUS: "Wako] Introducing MagCapture? Exosome Isolation KitPS.", SEOULIN BIOSCIENCE, 20 January 2016 (2016-01-20), XP009516892, Retrieved from the Internet
ALVES, NATHAN J. ET AL.: "Affinity Purification of Bacterial Outer Membrane Vesicles (OMVs) Utilizing a His-tag Mutant", RESEARCH IN MICROBIOLOGY, vol. 168, no. 2, 20 October 2016 (2016-10-20), pages 139 - 146, XP029920068
BERKOVSKY, AARON L. ET AL.: "Use of Metal-chelate Affinity Chromatography and Hydrophobic Interaction Chromatography for Purification of Placental Protein 12", JOURNAL OF CHROMATOGRAPHY B: BIOMEDICAL SCIENCES AND APPLICATIONS, vol. 656, no. 2, 17 June 1994 (1994-06-17), pages 432 - 435, XP026508359
NAKAI, WATARU ET AL.: "A Novel Affinity-based Method for the Isolation of Highly Purified Extracellular Vesicles", SCIENTIFIC REPORTS, vol. 6, no. 1, 23 September 2016 (2016-09-23), pages 1 - 11, XP055519274
See also references of EP 3569695A4
ANONYMOUS: "Wako] Introducing MagCapture? Exosome Isolation KitPS.", SEOULIN BIOSCIENCE, 20 January 2016 (2016-01-20), XP009516892, Retrieved from the Internet
ALVES, NATHAN J. ET AL.: "Affinity Purification of Bacterial Outer Membrane Vesicles (OMVs) Utilizing a His-tag Mutant", RESEARCH IN MICROBIOLOGY, vol. 168, no. 2, 20 October 2016 (2016-10-20), pages 139 - 146, XP029920068
BERKOVSKY, AARON L. ET AL.: "Use of Metal-chelate Affinity Chromatography and Hydrophobic Interaction Chromatography for Purification of Placental Protein 12", JOURNAL OF CHROMATOGRAPHY B: BIOMEDICAL SCIENCES AND APPLICATIONS, vol. 656, no. 2, 17 June 1994 (1994-06-17), pages 432 - 435, XP026508359
NAKAI, WATARU ET AL.: "A Novel Affinity-based Method for the Isolation of Highly Purified Extracellular Vesicles", SCIENTIFIC REPORTS, vol. 6, no. 1, 23 September 2016 (2016-09-23), pages 1 - 11, XP055519274
See also references of EP 3569695A4
Attorney, Agent or Firm:
ERUUM & LEEON INTELLECTUAL PROPERTY LAW FIRM (KR)
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