Title:
FE-S FUSION PROTEIN ACTING AS ELECTRON TRANSFER CHAIN, NOVEL CARBON MONOXIDE FORMATE REDOX ENZYME MEDIATED THROUGH FES FUSION PROTEIN, NOVEL STRAIN BCF12 DERIVED FROM THERMOCOCCUS WHEREIN ENZYME IS TRANSFORMED, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2020/101100
Kind Code:
A1
Abstract:
The present invention relates to a Fe-S fusion protein acting as an electron transfer chain, a novel carbon monoxide formate redox enzyme mediated through a FeS fusion protein, a novel strain BCF12 derived from Thermococcus wherein an enzyme is transformed, and use thereof. The Fe-S fusion protein of the present invention, by mediating itself, physically directly binds two different enzymes, so that electrons generated in either of the enzymes can be directly transferred to the other enzyme through a Fe-S cluster of the Fe-S fusion protein. Thus, by directly supplying the electrons necessary for the production of a target substance, a high-efficiency production reaction of the target substance without leakage of electrons generated by either of the enzymes is possible. In addition, the present invention has the advantages that the overall enzyme reaction rate and yield can be significantly improved by using a new electron transfer reaction that did not previously exist, and the stability of each enzyme can also be ensured by allowing the enzymes to be physically fixed within a cell.
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Inventors:
LEE JUNG-HYUN (KR)
LIM JAE KYU (KR)
LEE HYUN SOOK (KR)
KANG SUNG GYUN (KR)
KWON KAE KYOUNG (KR)
KIM YUN JAE (KR)
YANG JI-IN (KR)
LIM JAE KYU (KR)
LEE HYUN SOOK (KR)
KANG SUNG GYUN (KR)
KWON KAE KYOUNG (KR)
KIM YUN JAE (KR)
YANG JI-IN (KR)
Application Number:
PCT/KR2018/014807
Publication Date:
May 22, 2020
Filing Date:
November 28, 2018
Export Citation:
Assignee:
KOREA INST OCEAN SCI & TECH (KR)
International Classes:
C07K14/435; C12N9/02; C12N9/96; C12N15/52; C12N15/74; C12P7/40
Foreign References:
| KR20180005712A | 2018-01-16 | |||
| KR20160040088A | 2016-04-12 |
Other References:
HOFF, K. G.: "In vivo fluorescent detection of Fe-S clusters coordinated by human GRX2", CHEMISTRY & BIOLOGY, 2009, pages 1299 - 1308, XP026814721
MAZUMDER, T. K.: "Carbon monoxide conversion to formate by methanosarcina barkeri", BIOTECHNOLOGY LETTERS, vol. 7, no. 6, 1985, pages 377 - 382, XP055708455
LEE, H. S.: "The complete genome sequence of Thermococcus onnurineus NA1 reveals a mixed heterotrophic and carboxydotrophic metabolism", JOURNAL OF BACTERIOLOGY, vol. 190, no. 22, 2008, pages 7491 - 7499, XP055708458
MAZUMDER, T. K.: "Carbon monoxide conversion to formate by methanosarcina barkeri", BIOTECHNOLOGY LETTERS, vol. 7, no. 6, 1985, pages 377 - 382, XP055708455
LEE, H. S.: "The complete genome sequence of Thermococcus onnurineus NA1 reveals a mixed heterotrophic and carboxydotrophic metabolism", JOURNAL OF BACTERIOLOGY, vol. 190, no. 22, 2008, pages 7491 - 7499, XP055708458
Attorney, Agent or Firm:
AHN, Chang-woo (KR)
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