Title:
FEMALE SILKWORM LETHAL STRAIN OF BOMBYX MORI
Document Type and Number:
WIPO Patent Application WO/2016/076240
Kind Code:
A1
Abstract:
Established and provided is a strain of an insect belonging to the order Lepidoptera, said strain being able to be easily maintained and managed without interrupting the genetic background thereof, being applicable to genetic modification technology and easily and arbitrarily allowing male-specific breeding within a short period of time.
A female lethal strain can be obtained by transferring a gene expression vector, said gene expression vector encoding a mutated Masc gene that has cleavage resistance against Fem piRNA-Siwi complex and maintains the activity of the wild type Masc gene, into an insect belonging to the order Lepidoptera.
More Like This:
Inventors:
KASASHIMA MEGUMI (JP)
SEZUTSU HIDEKI (JP)
KATSUMA SUSUMU (JP)
KIUCHI TAKASHI (JP)
SUZUKI MASATAKA (JP)
SEZUTSU HIDEKI (JP)
KATSUMA SUSUMU (JP)
KIUCHI TAKASHI (JP)
SUZUKI MASATAKA (JP)
Application Number:
PCT/JP2015/081397
Publication Date:
May 19, 2016
Filing Date:
November 06, 2015
Export Citation:
Assignee:
NAT INST OF AGROBIO SCIENCES (JP)
International Classes:
C12N15/09; A01K67/033
Other References:
KIUCHI T. ET AL.: "A single female-specific piRNA is the primary determiner of sex in the silkworm", NATURE, vol. 509, 2014, pages 633 - 636, ISSN: 0028-0836
IMAMURA M. ET AL.: "Targeted gene expression using the GAL4/UAS system in the silkworm Bombyx mori", GENETICS, vol. 165, no. 3, 2003, pages 1329 - 1340, ISSN: 0016-6731
UCHINO K. ET AL.: "Evaluating promoter sequences for trapping an enhancer activity in the silkworm Bombyx mori", JOURNAL OF INSECT BIOTECHNOLOGY AND SERICOLOGY, vol. 75, no. 2, 2006, pages 85 - 97, ISSN: 1346-8073
TAKAHIRO SHIOTSUKI ET AL.: "Induction of precocious metamorphosis by a transgenic technique for mouse", JSCE NEWS, vol. 2005, no. 119, 2005, pages 8 - 12
DATABASE GenBank 30 May 2014 (2014-05-30), KAWAMOTO M. ET AL.: "Definition: Bombyx mori Masc mRNA for zinc finger protein, complete cds, strain: p50T", Database accession no. AB840788
TAKASHI KIUCHI ET AL.: "A single female-specific piRNA is the primary determiner of sex in the silkworm", LIFE SCIENCE SHINCHAKU RONBUN REVIEW, 3 June 2014 (2014-06-03), Retrieved from the Internet [retrieved on 20160104]
TAKASHI KIUCHI ET AL.: "Kaiko no Sei wa Tatta Hitotsu no Chiisana RNA ga Kettei suru -80 Nenrai no Nazo o Tsuini Kaimei! Kaiko no Sei Kettei Mechanism", GRADUATE SCHOOL OF AGRICULTURAL AND LIFE SCIENCES, 15 May 2014 (2014-05-15), Faculty of Agriculture, The University of Tokyo, Retrieved from the Internet [retrieved on 20160104]
HIROKI SAKAI ET AL.: "Kaiko ni Okeru Fem no Kino Kaiseki", DAI 37 KAI THE MOLECULAR BIOLOGY SOCIETY OF JAPAN NENKAI KOEN YOSHISHU, 7 November 2014 (2014-11-07), pages 1P-0641
SUSUMU KATSUMA ET AL.: "Kaiko Osu-ka Tanpakushitsu Masc no Kino Kaiseki", DAI 37 KAI THE MOLECULAR BIOLOGY SOCIETY OF JAPAN NENKAI KOEN YOSHISHU, 7 November 2014 (2014-11-07), pages 3P-0023
IMAMURA M. ET AL.: "Targeted gene expression using the GAL4/UAS system in the silkworm Bombyx mori", GENETICS, vol. 165, no. 3, 2003, pages 1329 - 1340, ISSN: 0016-6731
UCHINO K. ET AL.: "Evaluating promoter sequences for trapping an enhancer activity in the silkworm Bombyx mori", JOURNAL OF INSECT BIOTECHNOLOGY AND SERICOLOGY, vol. 75, no. 2, 2006, pages 85 - 97, ISSN: 1346-8073
TAKAHIRO SHIOTSUKI ET AL.: "Induction of precocious metamorphosis by a transgenic technique for mouse", JSCE NEWS, vol. 2005, no. 119, 2005, pages 8 - 12
DATABASE GenBank 30 May 2014 (2014-05-30), KAWAMOTO M. ET AL.: "Definition: Bombyx mori Masc mRNA for zinc finger protein, complete cds, strain: p50T", Database accession no. AB840788
TAKASHI KIUCHI ET AL.: "A single female-specific piRNA is the primary determiner of sex in the silkworm", LIFE SCIENCE SHINCHAKU RONBUN REVIEW, 3 June 2014 (2014-06-03), Retrieved from the Internet
TAKASHI KIUCHI ET AL.: "Kaiko no Sei wa Tatta Hitotsu no Chiisana RNA ga Kettei suru -80 Nenrai no Nazo o Tsuini Kaimei! Kaiko no Sei Kettei Mechanism", GRADUATE SCHOOL OF AGRICULTURAL AND LIFE SCIENCES, 15 May 2014 (2014-05-15), Faculty of Agriculture, The University of Tokyo, Retrieved from the Internet
HIROKI SAKAI ET AL.: "Kaiko ni Okeru Fem no Kino Kaiseki", DAI 37 KAI THE MOLECULAR BIOLOGY SOCIETY OF JAPAN NENKAI KOEN YOSHISHU, 7 November 2014 (2014-11-07), pages 1P-0641
SUSUMU KATSUMA ET AL.: "Kaiko Osu-ka Tanpakushitsu Masc no Kino Kaiseki", DAI 37 KAI THE MOLECULAR BIOLOGY SOCIETY OF JAPAN NENKAI KOEN YOSHISHU, 7 November 2014 (2014-11-07), pages 3P-0023
Attorney, Agent or Firm:
HIRAKI Yusuke et al. (JP)
Yusuke Hiraki (JP)
Yusuke Hiraki (JP)
Download PDF: