Title:
FERMENTATIVE PRODUCTION OF ISOPRENOIDS, LIPIDS OR STEROLS IN THE PRESENCE OF A LIPOPHILIC SOLVENT
Document Type and Number:
WIPO Patent Application WO/2023/214072
Kind Code:
A3
Abstract:
The present invention is related to fermentative production of isoprenoids, lipids, and sterols, particularly various forms of vitamin E and vitamin D3, as well as various polyunsaturated fatty acids (PUFAs), including derivatives or intermediates, comprising cultivation of a suitable host cell, such as fungal host cell, particularly oleaginous host cell, in an improved two-phase culture system. The two-phase culture system comprises in situ extraction of said isoprenoids, lipids or sterols, or a partial in situ extraction of said lipids, wherein a suitable host cell is cultivated in the presence of a carbon source and a lipophilic solvent, wherein the lipophilic solvent is different from the carbon source and with a minimal loss of solvent during the fermentation process. Preferably, the lipophilic solvent is selected from isoparaffins, such as e.g. from Isopar L or Isopar M.
Inventors:
VYAS VALMIK KANUBHAI (CH)
LAM ETHAN (CH)
HOUSTON PETER LOUIS (CH)
DOTEN REED CHADBOURNE (CH)
TICHOVOLSKY NATHAN CHARLES (CH)
JOHNSON MICHAEL BENJAMIN (CH)
DITTMAR JAMES (CH)
YAN QIANG (CH)
LAM ETHAN (CH)
HOUSTON PETER LOUIS (CH)
DOTEN REED CHADBOURNE (CH)
TICHOVOLSKY NATHAN CHARLES (CH)
JOHNSON MICHAEL BENJAMIN (CH)
DITTMAR JAMES (CH)
YAN QIANG (CH)
Application Number:
PCT/EP2023/062052
Publication Date:
February 01, 2024
Filing Date:
May 05, 2023
Export Citation:
Assignee:
DSM IP ASSETS BV (NL)
International Classes:
C12P5/00; B01D11/00; C12P7/64; C12P7/6463; C12P33/00
Domestic Patent References:
| WO2015162305A1 | 2015-10-29 | |||
| WO2019057998A1 | 2019-03-28 | |||
| WO2019058001A1 | 2019-03-28 | |||
| WO2022090548A1 | 2022-05-05 | |||
| WO2023067030A1 | 2023-04-27 |
Other References:
H GARDIN ET AL: "Biodegradation of xylene and butyl acetate using an aqueous-silicon oil two-phase system", BIODEGRADATION., vol. 10, no. 3, 1 June 1999 (1999-06-01), NL, pages 193 - 200, XP055473817, ISSN: 0923-9820, DOI: 10.1023/A:1008328318442
Attorney, Agent or Firm:
SEIBEL-THOMSEN, Nadja (CH)
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