Title:
FLAVIN-DEPENDENT OXIDASES HAVING CANNABINOID SYNTHASE ACTIVITY
Document Type and Number:
WIPO Patent Application WO/2023/168266
Kind Code:
A3
Abstract:
The present disclosure relates to a non-natural flavin-dependent oxidase that does not comprise a disulfide bond and that is capable of oxidative cyclization of a prenylated aromatic compound into a cannabinoid. In some embodiments, the non-natural flavin-dependent oxidase comprises: (i) at least 70% sequence identity to SEQ ID NO:3; and (ii) substitutions at amino acid positions Q275, C285, V323, E370, V372, N400, D404, and T438, wherein the amino acid positions correspond to SEQ ID NO:3. The present disclosure further relates to a polynucleotide, an expression construct, and an engineered cell for making the non-natural flavin-dependent oxidase. Also provided are a composition omprising the non-natural flavin-dependent oxidase; an isolated non-natural flavin-dependent oxidase and methods of making the same; a cell extract or cell culture medium comprising the non-natural flavin-dependent oxidase; and methods of making a cannabinoid.
Inventors:
HUDDLESTON JAMISON (US)
Application Number:
PCT/US2023/063479
Publication Date:
October 12, 2023
Filing Date:
March 01, 2023
Export Citation:
Assignee:
GENOMATICA INC (US)
International Classes:
C12N9/02; C12N15/52; C12P7/22; C12N15/83
Domestic Patent References:
| WO2021055597A1 | 2021-03-25 | |||
| WO2022051387A1 | 2022-03-10 |
Other References:
DATABASE UNIPROTKB ANONYMOUS : "U6A1G7 · U6A1G7_9ACTN", XP093101048, retrieved from UNIPROT
DATABASE UNIPROTKB ANONYMOUS : "A0A6F8YAS6 · A0A6F8YAS6_9ACTN", XP093101051, retrieved from UNIPROT
DATABASE UNIPROTKB ANONYMOUS : "A0A6F8YAS6 · A0A6F8YAS6_9ACTN", XP093101051, retrieved from UNIPROT
Attorney, Agent or Firm:
RAO, Minxi et al. (US)
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