Title:
FLP RECOMBINASE ACTIVATED BY STIMULATION, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2019/125072
Kind Code:
A1
Abstract:
The present invention relates to a Flp recombinase activated by stimulation and, more specifically, to: a Flp recombinase in which each fragment of a Flp recombinase split into two fragments is fused with a stimulus-induced dimerized protein, which binds in response to a stimulus, such that when a specific stimulus is applied, reversible activity is exhibited due to the binding of the stimulus-induced dimerization protein; and a use thereof. The activity of a Flp recombinase according to the present invention is regulated by a specific stimulus to allow gene expression to be be regulated according to target sites and locations, and thus Flp recombinase is useful for genetic manipulation in experimental animals.
Inventors:
HEO WONDO (KR)
PARK BYUNG OUK (KR)
JUNG HYEON JIN (KR)
PARK BYUNG OUK (KR)
JUNG HYEON JIN (KR)
Application Number:
PCT/KR2018/016517
Publication Date:
June 27, 2019
Filing Date:
December 21, 2018
Export Citation:
Assignee:
INST BASIC SCIENCE (KR)
International Classes:
C07K14/00; A01K67/027
Foreign References:
| CN103266092A | 2013-08-28 | |||
| US9206397B2 | 2015-12-08 | |||
| US20160264665A1 | 2016-09-15 |
Other References:
KAWANO, F.: "A photoactivatable Cre-loxP recombination system for optogenetic genome engineering", NATURE CHEMICAL BIOLOGY, vol. 12, 10 October 2016 (2016-10-10), pages 1059 - 1064, XP055506281
BAUMSCHLAGER, A.: "Dynamic blue light-inducible T7 RNA polymerases (Opto-T7RNAPs) for precise spatiotemporal gene expression control", ACS SYNTHETIC BIOLOGY, vol. 6, no. 11, 18 October 2017 (2017-10-18), pages 2157 - 2167, XP055621796
BAUMSCHLAGER, A.: "Dynamic blue light-inducible T7 RNA polymerases (Opto-T7RNAPs) for precise spatiotemporal gene expression control", ACS SYNTHETIC BIOLOGY, vol. 6, no. 11, 18 October 2017 (2017-10-18), pages 2157 - 2167, XP055621796
Attorney, Agent or Firm:
LEE, Cheo Young et al. (KR)
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