Title:
FLUORESCENCE INDICATOR UTILIZING FRET
Document Type and Number:
WIPO Patent Application WO/2005/036178
Kind Code:
A1
Abstract:
A novel fluorescence indicator for analyzing an intermolecular interaction or intramolecular structural change with the use of fluorescence resonance energy transfer (FRET). There is provided a fluorescence indicator having such a structure that a donor fluorescent protein and an acceptor fluorescent protein are linked to both ends of a target sequence for analyte wherein the three-dimensional structure of the indicator is changed by analyte liking to the target sequence or action on the target sequence to thereby induce fluorescence resonance energy transfer (FRET), characterized in that the donor fluorescent protein and/or acceptor fluorescent protein is a circular permutation variant fluorescent protein obtained by interchanging between N-terminus side amino acid sequence and C-terminus side amino acid sequence of wild type fluorescent protein or its variant protein and is a fluorescent protein having substantially the same fluorescence peak wavelength as that of the fluorescent protein before being subjected to the circular permutation variation.
Inventors:
MIYAWAKI ATSUSHI (JP)
NAGAI TAKEHARU (JP)
NAGAI TAKEHARU (JP)
Application Number:
PCT/JP2004/015671
Publication Date:
April 21, 2005
Filing Date:
October 15, 2004
Export Citation:
Assignee:
RIKEN (JP)
JAPAN SCIENCE & TECH AGENCY (JP)
MIYAWAKI ATSUSHI (JP)
NAGAI TAKEHARU (JP)
JAPAN SCIENCE & TECH AGENCY (JP)
MIYAWAKI ATSUSHI (JP)
NAGAI TAKEHARU (JP)
International Classes:
C12N1/15; C12N1/19; C12N1/21; G01N33/542; (IPC1-7): G01N33/68; G01N33/483; G01N33/84; C12N15/11; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C07K14/435; C07K19/00; C12Q1/02
Domestic Patent References:
| WO2000071565A2 | 2000-11-30 | |||
| WO1998040477A1 | 1998-09-17 |
Other References:
BAIRD G.S.: "Circular permutation and receptor insertion within green fluorescent proteins", PROC. NATL. ACAD. SCI. USA, vol. 96, no. 20, 28 September 1999 (1999-09-28), pages 11241 - 11246, XP002187230
NAGAI T. ET AL.: "Kokoritsu ni hakko kozo o toru GFP hen'itai no kaihatsu", BIOPHYSICS, vol. 42, no. 6, 2002, pages 305 - 308, XP002988135
TAKEMOTO K. ET AL.: "Spatio-temporal activation of caspase revealed by indicator that is insensitive to environmental effects", J. CELL. BIOL., vol. 160, no. 2, 20 January 2003 (2003-01-20), pages 235 - 243, XP002904546
NAGAI T. ET AL.: "Circularly permuted green fluorescent proteins engineered to sense Ca2+", PROC. NATL. ACAD. SCI. USA, vol. 98, no. 6, 13 March 2001 (2001-03-13), pages 3197 - 3202, XP002243309
NAGAI T. ET AL.: "Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins", PROC. NATL. ACAD. SCI. USA, vol. 101, no. 29, 20 July 2004 (2004-07-20), pages 10554 - 10559, XP002904547
BAIRD G. S. ET AL., PROC. NATL. ACAD. SCI. USA, vol. 96, 1999, pages 11241 - 11246
TOPELL S ET AL., FEBS LETT., vol. 457, 1999, pages 283 - 289
MIYAWAKI A., DEV. CELL, vol. 4, 2003, pages 295 - 305
NAKAI J. ET AL., NAT. BIOTECHNOL., vol. 19, 2001, pages 137 - 141
NAGAI T. ET AL., PROC. NATL. ACAD. SCI. USA, vol. 98, 2001, pages 3197 - 3202
PRASHER D.C. ET AL.: "Primary structure of the Aequorea victoria green fluorescent protein", GENE, vol. 111, 1992, pages 229 - 233, XP023542670, DOI: doi:10.1016/0378-1119(92)90691-H
See also references of EP 1686379A4
NAGAI T. ET AL.: "Kokoritsu ni hakko kozo o toru GFP hen'itai no kaihatsu", BIOPHYSICS, vol. 42, no. 6, 2002, pages 305 - 308, XP002988135
TAKEMOTO K. ET AL.: "Spatio-temporal activation of caspase revealed by indicator that is insensitive to environmental effects", J. CELL. BIOL., vol. 160, no. 2, 20 January 2003 (2003-01-20), pages 235 - 243, XP002904546
NAGAI T. ET AL.: "Circularly permuted green fluorescent proteins engineered to sense Ca2+", PROC. NATL. ACAD. SCI. USA, vol. 98, no. 6, 13 March 2001 (2001-03-13), pages 3197 - 3202, XP002243309
NAGAI T. ET AL.: "Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins", PROC. NATL. ACAD. SCI. USA, vol. 101, no. 29, 20 July 2004 (2004-07-20), pages 10554 - 10559, XP002904547
BAIRD G. S. ET AL., PROC. NATL. ACAD. SCI. USA, vol. 96, 1999, pages 11241 - 11246
TOPELL S ET AL., FEBS LETT., vol. 457, 1999, pages 283 - 289
MIYAWAKI A., DEV. CELL, vol. 4, 2003, pages 295 - 305
NAKAI J. ET AL., NAT. BIOTECHNOL., vol. 19, 2001, pages 137 - 141
NAGAI T. ET AL., PROC. NATL. ACAD. SCI. USA, vol. 98, 2001, pages 3197 - 3202
PRASHER D.C. ET AL.: "Primary structure of the Aequorea victoria green fluorescent protein", GENE, vol. 111, 1992, pages 229 - 233, XP023542670, DOI: doi:10.1016/0378-1119(92)90691-H
See also references of EP 1686379A4
Attorney, Agent or Firm:
SIKS & CO. (Kyobashi-Nisshoku Bldg. 8-7, Kyobashi 1-chom, Chuo-ku Tokyo, JP)
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