Title:
Fluorescent nucleotide analog hairpin formation for detection of nucleic acid hybridization
Document Type and Number:
WIPO Patent Application WO1998026093
Kind Code:
A3
Abstract:
This invention provides methods and compositions for the detection of nucleic acid interactions with other nucleic acids or with proteins. The methods generally utilize a nucleic acid (e.g., an oligonucleotide) that contains one or more fluorescent nucleotide analogues. The fluorescence of the nucleotide analogues is quenched (reduced) when they are incorporated into the oligonucleotide. Alteration of the normal conformation of the oligonucleotide by hybridization (e.g. to form a loop) or by protein binding reduces and/or eliminates the quench thereby causing a detectable increase in fluorescence.
Inventors:
HAWKINS MARY (US)
Application Number:
PCT/US1997/022448
Publication Date:
August 06, 1998
Filing Date:
December 10, 1997
Export Citation:
Assignee:
US GOV HEALTH & HUMAN SERV (US)
HAWKINS, MARY (US)
HAWKINS, MARY (US)
International Classes:
C12Q1/68; C12Q1/6816; C12Q1/686; (IPC1-7): C12Q1/68
Domestic Patent References:
| WO1992018650A1 | 1992-10-29 |
Foreign References:
| EP0601889A2 | 1994-06-15 | |||
| US5525711A | 1996-06-11 |
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