Title:
FLUORESCENT PROBE FOR DETECTING NAD(P)H IN MITOCHONDRIA, AND DETECTION METHOD USING SAME
Document Type and Number:
WIPO Patent Application WO/2020/036329
Kind Code:
A1
Abstract:
The present invention relates to a fluorescent probe for detecting NAD(P)H in mitochondria, and a detection method using same. A probe of the present invention exhibits high selectivity and sensitivity to NAD(P)H and emits strong red fluorescence in the presence of NAD(P)H. Therefore, the probe exhibits predominant accumulation in the mitochondria of living cells, is usable in the detection and quantification of NAD(P)H in the mitochondria, and can provide a selective image of the mitochondria in real time, thereby being effectively usable for biological pathway studies and the identification of pathological diagnoses and the like. In addition, the present invention can be used to differentiate cancer cells from normal cells on the basis of mapping of the intensity of NAD(P)H-dependent fluorescence in mitochondria.
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Inventors:
LEE MIN HEE (KR)
SHIN DONG SIK (KR)
JOO JIN HUI (KR)
YOUN DA YOUNG (KR)
CHANG MIN JUNG (KR)
YOON SHIN A (KR)
SHIN DONG SIK (KR)
JOO JIN HUI (KR)
YOUN DA YOUNG (KR)
CHANG MIN JUNG (KR)
YOON SHIN A (KR)
Application Number:
PCT/KR2019/009056
Publication Date:
February 20, 2020
Filing Date:
July 23, 2019
Export Citation:
Assignee:
INDUSTRY ACADEMIC COOPERATION FOUNDATION SOOKMYUNG WOMENS UNIV (KR)
International Classes:
G01N33/48; C07C381/00; C07D221/12; C09K11/06; G01N21/64; G01N21/76; G01N31/22; G01N33/483
Foreign References:
| US20160289252A1 | 2016-10-06 |
Other References:
JIN, F.: "Assembly, crystal structures, and luminescent properties of three new thiocyanate-bridging mercury(II) coordination polymers", JOURNAL OF COORDINATION CHEMISTRY, vol. 66, no. 20, 2013, pages 3686 - 3696, XP055688189, DOI: 10.1080/00958972.2013.851383
JIN, F.: "Diverse structural Ag (I) supramolecular complexes constructed from multidentate dicyanoisophorone-based ligands: structures and enhanced luminescence", CRYSTAL GROWTH & DESIGN, vol. 13, no. 5, 2013, pages 1978 - 1987, XP055688191, DOI: 10.1021/cg400025j
AN, RUIBING ET AL.: "A ''turn-on'' fluorescent and colorimetric sensor for selective detection of Cu2+ in aqueous media and living cells", SENSORS AND ACTUATORS B: CHEMICAL, vol. 222, 2016, pages 48 - 54, XP055688193, DOI: 10.1016/j.snb.2015.08.035
JOO, J. H.: "Mitochondria-targetable red-emitting probe for real-time fluorescence monitoring of NAD(P)H in live cells", DYES AND PIGMENTS, 14 May 2019 (2019-05-14), pages 1 - 7, XP085741295, DOI: 10.1016/j.dyepig.2019.107561
JIN, F.: "Diverse structural Ag (I) supramolecular complexes constructed from multidentate dicyanoisophorone-based ligands: structures and enhanced luminescence", CRYSTAL GROWTH & DESIGN, vol. 13, no. 5, 2013, pages 1978 - 1987, XP055688191, DOI: 10.1021/cg400025j
AN, RUIBING ET AL.: "A ''turn-on'' fluorescent and colorimetric sensor for selective detection of Cu2+ in aqueous media and living cells", SENSORS AND ACTUATORS B: CHEMICAL, vol. 222, 2016, pages 48 - 54, XP055688193, DOI: 10.1016/j.snb.2015.08.035
JOO, J. H.: "Mitochondria-targetable red-emitting probe for real-time fluorescence monitoring of NAD(P)H in live cells", DYES AND PIGMENTS, 14 May 2019 (2019-05-14), pages 1 - 7, XP085741295, DOI: 10.1016/j.dyepig.2019.107561
Attorney, Agent or Firm:
TAEBAEK INTELLECTUAL PROPERTY LAW FIRM (KR)
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