FLUORESCENT PROBE FOR PLASMA CELL IDENTIFICATION AND ISOLATION, AND PLASMA CELL IDENTIFICATION OR ISOLATION METHOD USING THE PROBE

Title:

FLUORESCENT PROBE FOR PLASMA CELL IDENTIFICATION AND ISOLATION, AND PLASMA CELL IDENTIFICATION OR ISOLATION METHOD USING THE PROBE

Document Type and Number:

WIPO Patent Application WO/2011/118579

Kind Code:

A1

Abstract:

Disclosed is a means which can isolate plasma cells and plasmablasts efficiently and with high purity, from mammals and birds, without using a cell surface marker. Further disclosed is a fluorescent probe wherein the staining selectivity relative to the endoplasmic reticulum of cells is higher than the staining selectivity relative to cell organelles other than the endoplasmic reticulum. Also disclosed is a method for identifying plasma cells and plasmablasts which includes using this probe to stain cells originating from lymph node tissue or similar, and identifying plasma cells and plasmablasts on the basis of the fluorescence intensity from the stained cells. Also disclosed is a probe wherein the staining selectivity relative to cell nuclei is higher than the staining selectivity relative to cell organelles other than the cell nuclei. Also disclosed is a method for identifying plasma cells and plasmablasts in lymph node tissue or similar which includes using this probe to stain cells originating from lymph node tissue or similar, and identifying plasma cells and plasmablasts on the basis of the fluorescence intensity from the stained cells.

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Inventors:

KUROSAWA NOBUYUKI (JP)
ISOBE MASAHARU (JP)

Application Number:

PCT/JP2011/056831

Publication Date:

September 29, 2011

Filing Date:

March 22, 2011

Export Citation:

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Assignee:

NAT UNIV CORP UNIV TOYAMA (JP)
KUROSAWA NOBUYUKI (JP)
ISOBE MASAHARU (JP)

International Classes:

C12Q1/04; C12N5/078; C12N5/0781; G01N21/64; G01N33/48; G01N33/483

Foreign References:

US20100068752A1

2010-03-18

Other References:

"Invitrogen 2004-2005 Nenban Catalog", CELL BIOLOGY & IMAGING, 2004, pages 1-44,1-49, XP008168590
TORU MURAKAMI ET AL.: "Cell biological applications of fluorescent probes", KENBIKYO, vol. 42, no. 1, 2007, pages 65 - 68, XP008166612
"SYTO® Red Fluorescent Nucleic Acid Stains.", MOLECULAR PROBES PRODUCT INFORMATION., 15 January 2001 (2001-01-15), XP002430592, Retrieved from the Internet [retrieved on 20110608]
See also references of EP 2551352A4
SANDERSON, R. D; LALOR, P.; BERNFIELD, M. B: "lymphocytes express and lose syndecan at specific stages of differentiation", CELL REGULATION, vol. 1, 1989, pages 27 - 35, XP055173506, DOI: doi:10.1091/mbc.1.1.27
HORST, A.HUNZELMANN; N. ARCE; S. HERBER; M. MANZ; R. A. RADBRUCH; A.NISCHT; R.SCHMITZ; J. ASSENMACHER, M. CLIN. EXP. IMMUNOL., vol. 130, pages 370 - 378
HANSCH C; LEO AJ.: "Exploring QSAR: Fundamentals and Applications in Chemistry and Biology", 1995, AMERICAN CHEMICAL SOCIETY, pages: 160
MORRALL SW; HERZOG RR; KLOEPPER-SAMS P; ROSEN MJ.: "Proc 4th World Surfactants Congress", vol. 3, 1996, AEPSAT: BARCELONA, article "Octanol/water partitioning of surfactants and its relevance to toxicity and environmental behavior", pages: 220 - 227

Attorney, Agent or Firm:

SIKs & Co. (JP)
Patent business corporation patent firm Sykes (JP)

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