Title:
FLUORESCENT PROTEIN SENSOR CAPABLE OF QUANTITATIVELY MEASURING FREE METHIONINE-R-SULFOXIDE, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/103124
Kind Code:
A1
Abstract:
The present invention relates to a fluorescent protein sensor capable of quantitatively measuring free methionine-R-sulfoxide, and to a use thereof. Specifically, the present invention relates to: a recombinant protein which is for a fluorescent biosensor for detecting free methionine-R-sulfoxide, and in which Thioredoxin 1 protein, cpYFP protein, and fRMsr protein are sequentially linked; a fluorescent biosensor which is for detecting free methionine-R-sulfoxide, and which includes the recombinant protein; a composition for detection; a method for quantitatively analyzing free methionine-R-sulfoxide; a method for providing information for diagnosing oxidative stress-related diseases; and a method for screening therapeutic agents for oxidative stress-related diseases. The recombinant protein for a fluorescent biosensor according to the present invention can quantitatively analyze, through fluorescence spectrum analysis, free methionine-R-sulfoxide generated by oxidative stress, and thus can be useful for a fluorescent biosensor for diagnosing oxidative stress diseases, a method for screening an agent for treating oxidative stress diseases, and a method for diagnosing disease prognosis after drug or surgical treatment.
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Inventors:
LEE BYUNG CHEON (KR)
ROH YEON JIN (KR)
KIM SEA HYUN (KR)
ROH YEON JIN (KR)
KIM SEA HYUN (KR)
Application Number:
PCT/KR2021/016253
Publication Date:
May 19, 2022
Filing Date:
November 09, 2021
Export Citation:
Assignee:
UNIV KOREA RES & BUS FOUND (KR)
International Classes:
C07K14/47; C12N9/02; C12N15/62; C12N15/85; G01N21/64; G01N33/483
Foreign References:
| US20170049911A1 | 2017-02-23 |
Other References:
KIM, SEAHYUN; LEE, HAE MIN; LEE, BUNG CHEON: "PBM-62 Development of fluorescence-based biosensor using fRMsr for detecting accumulated damage by oxidative stress", 2019 INTERNATIONAL SYMPOSIUM AND ANNUAL MEETING OF THE KSABC, JUNE 20-22; BUSAN, KOREA, 1 January 2019 (2019-01-01) - 22 June 2019 (2019-06-22), pages 187, XP009536751
KIM, SEAHYUN; LEE, HAE MIN; LEE, BYUNG CHEON: "PBM-40 Development of Biosensor using fRMsr for monitoring oxidative stress via free methionine oxidation", 2018 INTERNATIONAL SYMPOSIUM AND ANNUAL MEETING OF THE KSABC; JUNE 18-20, 2018; ICC JEJU, KOREA, 1 January 2018 (2018-01-01), pages 191, XP009536750
TARRAGO LIONEL, PÉTERFI ZALÁN, LEE BYUNG CHEON, MICHEL THOMAS, GLADYSHEV VADIM N: "Monitoring methionine sulfoxide with stereospecific mechanism-based fluorescent sensors", NATURE CHEMICAL BIOLOGY, vol. 11, no. 5, 1 May 2015 (2015-05-01), New York, pages 332 - 338, XP055913398, ISSN: 1552-4450, DOI: 10.1038/nchembio.1787
PÉTERFI ZALÁN; TARRAGO LIONEL; GLADYSHEV VADIM N: "Practical guide for dynamic monitoring of protein oxidation using genetically encoded ratiometric fluorescent biosensors of methionine sulfoxide", METHODS, ACADEMIC PRESS, NL, vol. 109, 23 June 2016 (2016-06-23), NL , pages 149 - 157, XP029772458, ISSN: 1046-2023, DOI: 10.1016/j.ymeth.2016.06.022
CHOI DONG WOOK, ROH YEON JIN, KIM SEAHYUN, LEE HAE MIN, KIM MINSEO, SHIN DONGHYUK, PARK JONG HO, CHO YONGMIN, PARK HEE HO, OK YONG: "Development of a novel fluorescent biosensor for dynamic monitoring of metabolic methionine redox status in cells and tissues", BIOSENSORS AND BIOELECTRONICS, vol. 178, no. 113031, 1 April 2021 (2021-04-01), Amsterdam , NL , pages 1 - 9, XP055913408, ISSN: 0956-5663, DOI: 10.1016/j.bios.2021.113031
KIM, SEAHYUN; LEE, HAE MIN; LEE, BYUNG CHEON: "PBM-40 Development of Biosensor using fRMsr for monitoring oxidative stress via free methionine oxidation", 2018 INTERNATIONAL SYMPOSIUM AND ANNUAL MEETING OF THE KSABC; JUNE 18-20, 2018; ICC JEJU, KOREA, 1 January 2018 (2018-01-01), pages 191, XP009536750
TARRAGO LIONEL, PÉTERFI ZALÁN, LEE BYUNG CHEON, MICHEL THOMAS, GLADYSHEV VADIM N: "Monitoring methionine sulfoxide with stereospecific mechanism-based fluorescent sensors", NATURE CHEMICAL BIOLOGY, vol. 11, no. 5, 1 May 2015 (2015-05-01), New York, pages 332 - 338, XP055913398, ISSN: 1552-4450, DOI: 10.1038/nchembio.1787
PÉTERFI ZALÁN; TARRAGO LIONEL; GLADYSHEV VADIM N: "Practical guide for dynamic monitoring of protein oxidation using genetically encoded ratiometric fluorescent biosensors of methionine sulfoxide", METHODS, ACADEMIC PRESS, NL, vol. 109, 23 June 2016 (2016-06-23), NL , pages 149 - 157, XP029772458, ISSN: 1046-2023, DOI: 10.1016/j.ymeth.2016.06.022
CHOI DONG WOOK, ROH YEON JIN, KIM SEAHYUN, LEE HAE MIN, KIM MINSEO, SHIN DONGHYUK, PARK JONG HO, CHO YONGMIN, PARK HEE HO, OK YONG: "Development of a novel fluorescent biosensor for dynamic monitoring of metabolic methionine redox status in cells and tissues", BIOSENSORS AND BIOELECTRONICS, vol. 178, no. 113031, 1 April 2021 (2021-04-01), Amsterdam , NL , pages 1 - 9, XP055913408, ISSN: 0956-5663, DOI: 10.1016/j.bios.2021.113031
Attorney, Agent or Firm:
NAM & NAM (KR)
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