Title:
FOCUSING A MICROSCOPE USING FLUORESCENT IMAGES
Document Type and Number:
WIPO Patent Application WO/2022/009104
Kind Code:
A3
Abstract:
Apparatus and methods are described for use with a microscope (24) that acquires images of a bodily sample disposed in a sample carrier (22). A fluorescent, microscopic image of a given x-y region is acquired, while a focal plane of the microscope (24) is set at an estimated optimum focal plane. A focusing region-of-interest is identified within the acquired image, based on fluorescent entities that are disposed within the focusing region-of interest. A set of fluorescent focusing images of the region-of-interest are acquired with the microscope (24) focused at respective focusing levels. An optimum focal plane at which to acquire a microscopic image of the region is determined, at least partially based upon a relationship between a contrast-indicative parameter of each of the images and the focusing level at which the microscope (24) was focused when the image was acquired. Other applications are also described.
More Like This:
| WO/2012/000923 | METHOD AND DEVICE FOR LIGHT-MICROSCOPIC IMAGING OF A SAMPLE STRUCTURE |
| WO/2004/003601 | HIGH TRANSMISSION OPTICAL INSPECTION TOOLS |
Inventors:
ZAIT AMIR (IL)
HOURI YAFIN ARNON (IL)
PADAN ROI (IL)
GLUCK DAN (IL)
RIMER OFIR (IL)
GROSS SAR (IL)
OFER LIOR (IL)
ESHEL YOCHAY SHLOMO (IL)
LEVY SARAH (IL)
POLLAK JOSEPH JOEL (IL)
HOURI YAFIN ARNON (IL)
PADAN ROI (IL)
GLUCK DAN (IL)
RIMER OFIR (IL)
GROSS SAR (IL)
OFER LIOR (IL)
ESHEL YOCHAY SHLOMO (IL)
LEVY SARAH (IL)
POLLAK JOSEPH JOEL (IL)
Application Number:
PCT/IB2021/056073
Publication Date:
February 17, 2022
Filing Date:
July 07, 2021
Export Citation:
Assignee:
S D SIGHT DIAGNOSTICS LTD (IL)
International Classes:
G02B21/16; G01N15/14; G01N21/64; G02B21/24; G02B21/36
Foreign References:
| US20050282292A1 | 2005-12-22 | |||
| US20120249770A1 | 2012-10-04 | |||
| CN110244444A | 2019-09-17 | |||
| US20080012978A1 | 2008-01-17 |
Other References:
PETER HAMM: "lnhaltsbasierte Autofokussierung in der automatisierten Mikroskopie (dissertation)", 1 January 2011 (2011-01-01), pages 110pp, XP055622015, Retrieved from the Internet [retrieved on 20190913]
ANONYMOUS: "MetaXpress 6 Software Guide Configuring Image-Based Autofocus During Acquisition", 29 July 2015 (2015-07-29), XP055838930, Retrieved from the Internet [retrieved on 20210908]
ANONYMOUS: "Micro-Manager User's Guide - Micro-Manager", 1 January 2016 (2016-01-01), XP055408760, Retrieved from the Internet [retrieved on 20170921]
ANONYMOUS: "MicroManager Documentation: SimpleAutofocus", 31 December 2009 (2009-12-31), XP055839097, Retrieved from the Internet [retrieved on 20210908]
HOOEVER KARL: "SimpleAutofocus.cpp - Micro-Manager Device Adapter code", 31 December 2009 (2009-12-31), XP055838960, Retrieved from the Internet [retrieved on 20210908]
ANONYMOUS: "MetaXpress 6 Software Guide Configuring Image-Based Autofocus During Acquisition", 29 July 2015 (2015-07-29), XP055838930, Retrieved from the Internet
ANONYMOUS: "Micro-Manager User's Guide - Micro-Manager", 1 January 2016 (2016-01-01), XP055408760, Retrieved from the Internet
ANONYMOUS: "MicroManager Documentation: SimpleAutofocus", 31 December 2009 (2009-12-31), XP055839097, Retrieved from the Internet
HOOEVER KARL: "SimpleAutofocus.cpp - Micro-Manager Device Adapter code", 31 December 2009 (2009-12-31), XP055838960, Retrieved from the Internet
Attorney, Agent or Firm:
BEIDER, Joel (IL)
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