Title:
FRUCTOSAMINE DEGLYCASE VECTOR, TRANSGENIC CELL LINE AND GENETICALLY ENGINEERED BACTERIUM EXPRESSING FRUCTOSAMINE DEGLYCASE, AND USE OF FRUCTOSAMINE DEGLYCASE
Document Type and Number:
WIPO Patent Application WO/2023/004772
Kind Code:
A1
Abstract:
The present invention relates to a fructosamine deglycase vector, a transgenic cell line and a genetically engineered bacterium expressing fructosamine deglycase, and the use of fructosamine deglycase, which belong to the technical field of biological enzymatic production. Provided in the present invention is a method for catalyzing the transfer of an amino group from an amino donor compound to an amino acceptor compound. The method comprises: catalyzing the transfer of an amino group from an amino donor compound to an amino acceptor compound by means of using an enzyme (fructosamine deglycase), or an expression vector or a cloning vector expressing the enzyme, or a transgenic cell line expressing the enzyme, or a genetically engineered bacterium expressing the enzyme. The method of the present invention has the advantages of a sufficient substrate source, no limitations on raw materials, mild preparation conditions, low environmental pollution, a high reaction specificity, few impurities in the system, ease of downstream separation and purification, and a low production cost.
Inventors:
WANG HUAIYING (CN)
Application Number:
PCT/CN2021/109679
Publication Date:
February 02, 2023
Filing Date:
July 30, 2021
Export Citation:
Assignee:
WUHAN BAIANHUIJI BIOTECHNOLOGY CO LTD (CN)
International Classes:
C12N1/21; C12P7/40; C12N5/10; C12N9/78; C12N15/70; C12P19/26
Foreign References:
| CN113046401A | 2021-06-29 | |||
| CN112852774A | 2021-05-28 | |||
| US5387109A | 1995-02-07 |
Other References:
DATABASE Protein 1 November 2016 (2016-11-01), ANONYMOUS: "Fructosamine deglycase FrlB [Bacillus subtilis]", XP055954458, retrieved from Genbank Database accession no. AOR99552
DATABASE GenBank 25 October 2013 (2013-10-25), ANONYMOUS: "ESD97983 fructokinase [Escherichia coli 908658]", XP009538610, retrieved from NCBI Database accession no. ESD97983
"GenBank", Database accession no. AOR99552.1
"Molecular Cloning Experimental Guidelines (Third Edition"
See also references of EP 4151742A4
DATABASE GenBank 25 October 2013 (2013-10-25), ANONYMOUS: "ESD97983 fructokinase [Escherichia coli 908658]", XP009538610, retrieved from NCBI Database accession no. ESD97983
"GenBank", Database accession no. AOR99552.1
"Molecular Cloning Experimental Guidelines (Third Edition"
See also references of EP 4151742A4
Attorney, Agent or Firm:
BEIJING GAOWO LAW FIRM (CN)
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