Title:
FRUCTOSE-1,6-DIPHOSPHATE OPTICAL PROBE, AND PREPARATION METHOD THEREFOR AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2024/094002
Kind Code:
A1
Abstract:
The present invention relates to a fructose diphosphate optical probe. Specifically, provided in the present invention is a fructose diphosphate optical probe, which comprises a fructose-1,6-diphosphate sensitive polypeptide and an optically active polypeptide, wherein the optically active polypeptide is located within the sequence of a fructose-1,6-diphosphate sensitive polypeptide, the fructose-1,6-diphosphate sensitive polypeptide is a fructose-1,6-diphosphate binding protein or a functional variant thereof, and the optically active polypeptide is a fluorescent protein or a functional variant thereof. The fluorescent probe of the present invention has a large dynamic change in terms of fluorescence and a good specificity, and can be used for high-throughput and quantitative detection of fructose diphosphate inside and outside a cell.
Inventors:
YANG YI (CN)
ZHAO YUZHENG (CN)
YAO JING (CN)
ZOU YEJUN (CN)
ZHANG LIJUAN (CN)
CHEN YAN (CN)
LI XIE (CN)
ZHAO YUZHENG (CN)
YAO JING (CN)
ZOU YEJUN (CN)
ZHANG LIJUAN (CN)
CHEN YAN (CN)
LI XIE (CN)
Application Number:
PCT/CN2023/128488
Publication Date:
May 10, 2024
Filing Date:
October 31, 2023
Export Citation:
Assignee:
UNIV EAST CHINA SCIENCE & TECH (CN)
International Classes:
C07K14/00; C07K19/00; C12N1/21; C12N15/62; C12N15/70; G01N21/00; G01N33/53; G01N33/533; G01N33/58; C12R1/19
Foreign References:
CN114057891A | 2022-02-18 | |||
CN113336855A | 2021-09-03 |
Other References:
SILVIA ZORRILLA: "Fructose-1,6-bisphosphate Acts Both as an Inducer and as a Structural Cofactor of the Central Glycolytic Genes Repressor (CggR)", BIOCHEMISTRY, AMERICAN CHEMICAL SOCIETY, vol. 46, no. 51, 1 December 2007 (2007-12-01), pages 14996 - 15008, XP093166231, ISSN: 0006-2960, DOI: 10.1021/bi701805e
THIERRY DOAN: "Regulation of the central glycolytic genes in Bacillus subtilis : binding of the repressor CggR to its single DNA target sequence is modulated by fructose‐1,6‐bisphosphate", MOLECULAR MICROBIOLOGY, WILEY-BLACKWELL PUBLISHING LTD, GB, vol. 47, no. 6, 1 March 2003 (2003-03-01), GB , pages 1709 - 1721, XP093166234, ISSN: 0950-382X, DOI: 10.1046/j.1365-2958.2003.03404.x
DATABASE PROTEIN 11 August 2019 (2019-08-11), ANONYMOUS: "gapA transcriptional regulator CggR [Bacillus atrophaeus]", XP093166237, Database accession no. WP_010789975.1
THIERRY DOAN: "Regulation of the central glycolytic genes in Bacillus subtilis : binding of the repressor CggR to its single DNA target sequence is modulated by fructose‐1,6‐bisphosphate", MOLECULAR MICROBIOLOGY, WILEY-BLACKWELL PUBLISHING LTD, GB, vol. 47, no. 6, 1 March 2003 (2003-03-01), GB , pages 1709 - 1721, XP093166234, ISSN: 0950-382X, DOI: 10.1046/j.1365-2958.2003.03404.x
DATABASE PROTEIN 11 August 2019 (2019-08-11), ANONYMOUS: "gapA transcriptional regulator CggR [Bacillus atrophaeus]", XP093166237, Database accession no. WP_010789975.1
Attorney, Agent or Firm:
SHANGHAI PATENT & TRADEMARK LAW OFFICE, LLC (CN)
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