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Title:
FRUCTOSE-1,6-DIPHOSPHATE OPTICAL PROBE, AND PREPARATION METHOD THEREFOR AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2024/094002
Kind Code:
A1
Abstract:
The present invention relates to a fructose diphosphate optical probe. Specifically, provided in the present invention is a fructose diphosphate optical probe, which comprises a fructose-1,6-diphosphate sensitive polypeptide and an optically active polypeptide, wherein the optically active polypeptide is located within the sequence of a fructose-1,6-diphosphate sensitive polypeptide, the fructose-1,6-diphosphate sensitive polypeptide is a fructose-1,6-diphosphate binding protein or a functional variant thereof, and the optically active polypeptide is a fluorescent protein or a functional variant thereof. The fluorescent probe of the present invention has a large dynamic change in terms of fluorescence and a good specificity, and can be used for high-throughput and quantitative detection of fructose diphosphate inside and outside a cell.

Inventors:
YANG YI (CN)
ZHAO YUZHENG (CN)
YAO JING (CN)
ZOU YEJUN (CN)
ZHANG LIJUAN (CN)
CHEN YAN (CN)
LI XIE (CN)
Application Number:
PCT/CN2023/128488
Publication Date:
May 10, 2024
Filing Date:
October 31, 2023
Export Citation:
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Assignee:
UNIV EAST CHINA SCIENCE & TECH (CN)
International Classes:
C07K14/00; C07K19/00; C12N1/21; C12N15/62; C12N15/70; G01N21/00; G01N33/53; G01N33/533; G01N33/58; C12R1/19
Foreign References:
CN114057891A2022-02-18
CN113336855A2021-09-03
Other References:
SILVIA ZORRILLA: "Fructose-1,6-bisphosphate Acts Both as an Inducer and as a Structural Cofactor of the Central Glycolytic Genes Repressor (CggR)", BIOCHEMISTRY, AMERICAN CHEMICAL SOCIETY, vol. 46, no. 51, 1 December 2007 (2007-12-01), pages 14996 - 15008, XP093166231, ISSN: 0006-2960, DOI: 10.1021/bi701805e
THIERRY DOAN: "Regulation of the central glycolytic genes in Bacillus subtilis : binding of the repressor CggR to its single DNA target sequence is modulated by fructose‐1,6‐bisphosphate", MOLECULAR MICROBIOLOGY, WILEY-BLACKWELL PUBLISHING LTD, GB, vol. 47, no. 6, 1 March 2003 (2003-03-01), GB , pages 1709 - 1721, XP093166234, ISSN: 0950-382X, DOI: 10.1046/j.1365-2958.2003.03404.x
DATABASE PROTEIN 11 August 2019 (2019-08-11), ANONYMOUS: "gapA transcriptional regulator CggR [Bacillus atrophaeus]", XP093166237, Database accession no. WP_010789975.1
Attorney, Agent or Firm:
SHANGHAI PATENT & TRADEMARK LAW OFFICE, LLC (CN)
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