Title:
FRUCTOSE-BISPHOSPHATE ALDOLASE REGULATORY SEQUENCES FOR GENE EXPRESSION IN OLEAGINOUS YEAST
Document Type and Number:
WIPO Patent Application WO2005049805
Kind Code:
A3
Abstract:
The regulatory sequences (i.e., promoter regions, introns and enhancers) associated with the Yarrowia lipolytica gene encoding fructose bis-phospate aldolase (FBA1) have been found to be particularly effective for the expression of heterologus genes in oleaginous yeast. The promoter regions of the invention have been shown to drive high-level expression of genes involved in the production of ?-3 and ?-6 fatty acids.
Inventors:
POLLAK DANA M WALTERS (US)
ZHU QUINN QUN (US)
ZHU QUINN QUN (US)
Application Number:
PCT/US2004/038571
Publication Date:
February 16, 2006
Filing Date:
November 12, 2004
Export Citation:
Assignee:
DU PONT (US)
POLLAK DANA M WALTERS (US)
ZHU QUINN QUN (US)
POLLAK DANA M WALTERS (US)
ZHU QUINN QUN (US)
International Classes:
C12N1/18; C12N9/04; C12N9/10; C12N9/26; C12N15/74; C12P7/64; C12N; (IPC1-7): C12P21/00
Other References:
COMPAGNO C. ET AL.: "The promoter of Saccharomyces cerevisiae FBA1 gene contains a single ositive upstream regulatory element.", FEBS LETTERS, vol. 293, no. 1-2, November 1991 (1991-11-01), pages 97 - 100, XP002993280
MADZAK C. ET AL.: "Functional analysis of upstream regulating regions from the Yarrowia lipolytica XPR2 promoter.", MICROBIOLOGY, vol. 145, January 1999 (1999-01-01), pages 75 - 87, XP002993299
JURETZEK T. ET AL.: "Vectors forgene expresson and amplifcation in the yeast Yarrowia lipolytica.", YEAST, vol. 18, January 2001 (2001-01-01), pages 97 - 113, XP008025954
MADZAK C. ET AL.: "Functional analysis of upstream regulating regions from the Yarrowia lipolytica XPR2 promoter.", MICROBIOLOGY, vol. 145, January 1999 (1999-01-01), pages 75 - 87, XP002993299
JURETZEK T. ET AL.: "Vectors forgene expresson and amplifcation in the yeast Yarrowia lipolytica.", YEAST, vol. 18, January 2001 (2001-01-01), pages 97 - 113, XP008025954
Download PDF:
Previous Patent: ENGINEERING ENZYMES THROUGH GENETIC SELECTION
Next Patent: NOVEL NUCLEIC ACIDS AND POLYPEPTIDES
Next Patent: NOVEL NUCLEIC ACIDS AND POLYPEPTIDES