Title:
FULLY HUMAN BROAD-SPECTRUM HIGH-NEUTRALIZING-ACTIVITY MONOCLONAL ANTIBODY AGAINST SARS-COV-2 AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/040627
Kind Code:
A1
Abstract:
A fully human monoclonal antibody ZWD12 against SARS-CoV-2. The antibody is obtained by screening using a flow sorting-single cell PCR technology and has a unique CDR, and the antigen recognition epitope of the antibody is located in an RBD region of S1 protein. The EC50 of the antibody against the wild type of SARS-CoV-2 is 0.103 μg/mL, and the EC50 neutralizing the Beta strain is 0.069 μg/mL, and the EC50 neutralizing the Delta strain is 0.079 μg/mL, showing that ZWD12 has broad-spectrum neutralizing activity against the current major mutant strains.
Inventors:
CHEN WEI (CN)
YU CHANGMING (CN)
CHI XIANGYANG (CN)
SUN HANCONG (CN)
ZHANG GUANYING (CN)
ZHANG JUN (CN)
LI JIANMIN (CN)
FAN PENGFEI (CN)
WANG BUSEN (CN)
ZAI XIAODONG (CN)
ZHANG ZHE (CN)
FANG TING (CN)
FU LING (CN)
CHEN ZHENGSHAN (CN)
HAO MENG (CN)
CHEN YI (CN)
XU JINGHAN (CN)
YU CHANGMING (CN)
CHI XIANGYANG (CN)
SUN HANCONG (CN)
ZHANG GUANYING (CN)
ZHANG JUN (CN)
LI JIANMIN (CN)
FAN PENGFEI (CN)
WANG BUSEN (CN)
ZAI XIAODONG (CN)
ZHANG ZHE (CN)
FANG TING (CN)
FU LING (CN)
CHEN ZHENGSHAN (CN)
HAO MENG (CN)
CHEN YI (CN)
XU JINGHAN (CN)
Application Number:
PCT/CN2022/114962
Publication Date:
March 23, 2023
Filing Date:
August 25, 2022
Export Citation:
Assignee:
ACAD OF MILITARY MEDICAL SCIENCE PLA (CN)
International Classes:
C12N15/13; A61K39/42; A61P31/14; C07K16/10
Domestic Patent References:
| WO2021180218A1 | 2021-09-16 | |||
| WO2021169932A1 | 2021-09-02 |
Foreign References:
| CN113735969A | 2021-12-03 | |||
| CN111303280A | 2020-06-19 | |||
| CN111732655A | 2020-10-02 |
Other References:
BORDOLOI DEVIVASHA, XU ZIYANG, HO MICHELLE, PURWAR MANSI, BHOJNAGARWALA PRATIK, CASSEL JOEL, GIRON LEILA B., WALKER SUSANNE, KULKA: "Identification of Novel Neutralizing Monoclonal Antibodies against SARS-CoV-2 Spike Glycoprotein", ACS PHARMACOLOGY & TRANSLATIONAL SCIENCE, vol. 4, no. 4, 13 August 2021 (2021-08-13), pages 1349 - 1361, XP093048976, ISSN: 2575-9108, DOI: 10.1021/acsptsci.1c00058
GÜTTLER THOMAS, AKSU METIN, DICKMANNS ANTJE, STEGMANN KIM M., GREGOR KATHRIN, REES RENATE, TAXER WALTRAUD, RYMARENKO OLEH, SCHÜNEM: "Neutralization of SARS‐CoV‐2 by highly potent, hyperthermostable, and mutation‐tolerant nanobodies", THE EMBO JOURNAL / EUROPEAN MOLECULAR BIOLOGY ORGANIZATION, IRL PRESS, OXFORD, vol. 40, no. 19, Oxford , pages 107985, XP055858032, ISSN: 0261-4189, DOI: 10.15252/embj.2021107985
GÜTTLER THOMAS, AKSU METIN, DICKMANNS ANTJE, STEGMANN KIM M., GREGOR KATHRIN, REES RENATE, TAXER WALTRAUD, RYMARENKO OLEH, SCHÜNEM: "Neutralization of SARS‐CoV‐2 by highly potent, hyperthermostable, and mutation‐tolerant nanobodies", THE EMBO JOURNAL / EUROPEAN MOLECULAR BIOLOGY ORGANIZATION, IRL PRESS, OXFORD, vol. 40, no. 19, Oxford , pages 107985, XP055858032, ISSN: 0261-4189, DOI: 10.15252/embj.2021107985
Attorney, Agent or Firm:
BEIJING ZHONGTIAN LAWFIRM (CN)
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