Title:
THE FUNCTIONAL DISRUPTION OF AVIAN IMMUNOGLOBULIN GENES
Document Type and Number:
WIPO Patent Application WO2003081992
Kind Code:
A3
Abstract:
A transgenic chicken is disclosed having disrupted endogenous immunoglobulin production. In one embodiment, a targeting construct is stably integrated into the genome of the chicken by homologous recombination in embryonic stem cells, and injection of the engineered embryonic stem cells into recipient embryos, thereby knocking out the endogenous immunoglobulin gene locus in resulting animals. The targeted disruption of the locus in embryonic stem cells is particularly useful in combination with the insertion of geneticelements encoding exogenous immunoglobulin molecules. After these chickens are crossbred, a line of chickens is produced that has a reduction of endogenous immunoglobulin molecule production.
Inventors:
ETCHES ROBERT J
KAY ROBERT M
ZHU LEI
KAY ROBERT M
ZHU LEI
Application Number:
PCT/US2003/009178
Publication Date:
March 25, 2004
Filing Date:
March 24, 2003
Export Citation:
Assignee:
ORIGEN THERAPEUTICS (US)
International Classes:
A01K67/00; A01K67/027; C07K16/00; C12N15/85; A01K; (IPC1-7): A01K67/00
Foreign References:
| US6066778A | 2000-05-23 | |||
| US5939598A | 1999-08-17 | |||
| US5998209A | 1999-12-07 | |||
| US6143559A | 2000-11-07 | |||
| US6316600B1 | 2001-11-13 |
Other References:
IVARIE: "Avian transgenesis progress towards the promise", TRENDS IN BIOTECHNOLOGY, vol. 21, no. 1, January 2003 (2003-01-01), pages 14 - 19, XP004397632
ISHIDA ET AL.: "Production of human monoclonal and polyclonal antibodies in transchromo animals", CLONING AND STEM CELLS, vol. 4, no. 1, 2002, pages 91 - 102, XP002971700
DAVIES ET AL.: "Selection of specific phage-display antibodies using libraries derived from chicken immunoglobulin genes", J. IMMUNOL., vol. 186, 1995, pages 125 - 135, XP004021146
MOHAMMED ET AL.: "Deposition of genetically engineered human antibodies into the egg yolk of hens", IMMUNOTECH., vol. 4, 1998, pages 115 - 125, XP000992749
BEZZUBOVA ET AL.: "Gene conversion in the chicken immunoglobulin locus: a paradigm of homologous recombination in higher eukaryotes", EXPERIENTIA, vol. 50, 1994, pages 270 - 276, XP002084452
See also references of EP 1487260A4
ISHIDA ET AL.: "Production of human monoclonal and polyclonal antibodies in transchromo animals", CLONING AND STEM CELLS, vol. 4, no. 1, 2002, pages 91 - 102, XP002971700
DAVIES ET AL.: "Selection of specific phage-display antibodies using libraries derived from chicken immunoglobulin genes", J. IMMUNOL., vol. 186, 1995, pages 125 - 135, XP004021146
MOHAMMED ET AL.: "Deposition of genetically engineered human antibodies into the egg yolk of hens", IMMUNOTECH., vol. 4, 1998, pages 115 - 125, XP000992749
BEZZUBOVA ET AL.: "Gene conversion in the chicken immunoglobulin locus: a paradigm of homologous recombination in higher eukaryotes", EXPERIENTIA, vol. 50, 1994, pages 270 - 276, XP002084452
See also references of EP 1487260A4
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