Title:
FUNCTIONAL NANOMAGNETIC BEAD FOR ENRICHING POTATO ESTER ACYL HYDROLASE, PREPARATION METHOD THEREFOR AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2021/093588
Kind Code:
A1
Abstract:
A preparation method for a functional nanomagnetic bead for enriching potato ester acyl hydrolase, comprising the following steps: mixing a carboxylated nanomagnetic bead with ethyl-dimethylamine-propylcarbodiimide; activating the nanomagnetic bead; and coupling the activated nanomagnetic bead with aleuria aurantia lectin, so as to obtain the functional nanomagnetic bead. The present invention also provides use of a functional nanomagnetic bead coupled with aleuria aurantia lectin for enriching the potato ester acyl hydrolase, comprising the following steps: extracting crude protein from potato starch waste water; performing impurity removal and elution to obtain an eluate; using the functional nanomagnetic bead to enrich the potato ester acyl hydrolase in the eluate; and performing elution and purification to obtain the potato ester acyl hydrolase. By using the functional nanomagnetic bead prepared by the present method to enrich or adsorb and immobilize the potato ester acyl hydrolase, the time for enriching the potato ester acyl hydrolase can be shortened, the purity and enzyme catalytic performance of the potato ester acyl hydrolase can be improved, and the biological activity thereof can be retained.
Inventors:
WU JINHONG (CN)
WU QIAOYU (CN)
WENG QING (CN)
XU JINGSHEN (CN)
ZHOU MI (CN)
WANG ZHENGWU (CN)
YU YING (CN)
WU QIAOYU (CN)
WENG QING (CN)
XU JINGSHEN (CN)
ZHOU MI (CN)
WANG ZHENGWU (CN)
YU YING (CN)
Application Number:
PCT/CN2020/124301
Publication Date:
May 20, 2021
Filing Date:
October 28, 2020
Export Citation:
Assignee:
UNIV SHANGHAI JIAOTONG (CN)
International Classes:
C12N9/20; B01J20/281; C07K1/22
Foreign References:
| CN110846295A | 2020-02-28 | |||
| CN104772124A | 2015-07-15 | |||
| CN107430126A | 2017-12-01 | |||
| US20120258484A1 | 2012-10-11 | |||
| CN106093021A | 2016-11-09 |
Other References:
WU, QIAOYU ET AL.: "Investigation on the Characteristics of the Activity of Potato Lipid Acyl Hydrolase", FOOD AND MACHINERY, vol. 35, no. 11, 17 October 2019 (2019-10-17), pages 41 - 46, XP055811683
LI, FENG ET AL.: "Enrichment of Glycoproteins in Human Serum Using Concanavalin A-Functionalized Magnetic Nanoparticles and Identification by Mass Spectrometry)", CHINESE JOURNAL OF CHROMATOGRAPHY, vol. 32, no. 4, 30 April 2014 (2014-04-30), pages 369 - 375, XP055811686
NORTON PAMELA, COMUNALE MARY ANN, HERRERA HARMIN, WANG MENGJUN, HOUSER JOSEF, WIMMEROVA MICHAELA, ROMANO PATRICK R., MEHTA ANAND: "Development and application of a novel recombinant Aleuria aurantia lectin with enhanced core fucose binding for identification of glycoprotein biomarkers of hepatocellular carcinoma", PROTEOMICS, WILEY-VCH VERLAG , WEINHEIM, DE, vol. 16, no. 24, 1 December 2016 (2016-12-01), DE, pages 3126 - 3136, XP055811692, ISSN: 1615-9853, DOI: 10.1002/pmic.201600064
AHN YEONG HEE, SHIN PARK MIN, KIM YONG-SAM, OH NA REE, JI EUN SUN, KIM KWANG HOE, LEE YEON JUNG, KIM SUNG HO, YOO JONG SHIN: "Quantitative analysis of aberrant protein glycosylation in liver cancer plasma by AAL-enrichment and MRM mass spectrometry", ANALYST, ROYAL SOCIETY OF CHEMISTRY, UK, vol. 138, no. 21, 1 January 2013 (2013-01-01), UK, pages 6454, XP055812170, ISSN: 0003-2654, DOI: 10.1039/c3an01126g
SCHMIDT JESPER MALLING; DAMGAARD HENRIETTE; GREVE-POULSEN MATHIAS; LARSEN LOTTE BACH; HAMMERSHØJ MARIANNE: "Foam and emulsion properties of potato protein isolate and purified fractions", FOOD HYDROCOLLOIDS, ELSEVIER BV, NL, vol. 74, 7 August 2017 (2017-08-07), NL, pages 367 - 378, XP085202090, ISSN: 0268-005X, DOI: 10.1016/j.foodhyd.2017.07.032
LI, FENG ET AL.: "Enrichment of Glycoproteins in Human Serum Using Concanavalin A-Functionalized Magnetic Nanoparticles and Identification by Mass Spectrometry)", CHINESE JOURNAL OF CHROMATOGRAPHY, vol. 32, no. 4, 30 April 2014 (2014-04-30), pages 369 - 375, XP055811686
NORTON PAMELA, COMUNALE MARY ANN, HERRERA HARMIN, WANG MENGJUN, HOUSER JOSEF, WIMMEROVA MICHAELA, ROMANO PATRICK R., MEHTA ANAND: "Development and application of a novel recombinant Aleuria aurantia lectin with enhanced core fucose binding for identification of glycoprotein biomarkers of hepatocellular carcinoma", PROTEOMICS, WILEY-VCH VERLAG , WEINHEIM, DE, vol. 16, no. 24, 1 December 2016 (2016-12-01), DE, pages 3126 - 3136, XP055811692, ISSN: 1615-9853, DOI: 10.1002/pmic.201600064
AHN YEONG HEE, SHIN PARK MIN, KIM YONG-SAM, OH NA REE, JI EUN SUN, KIM KWANG HOE, LEE YEON JUNG, KIM SUNG HO, YOO JONG SHIN: "Quantitative analysis of aberrant protein glycosylation in liver cancer plasma by AAL-enrichment and MRM mass spectrometry", ANALYST, ROYAL SOCIETY OF CHEMISTRY, UK, vol. 138, no. 21, 1 January 2013 (2013-01-01), UK, pages 6454, XP055812170, ISSN: 0003-2654, DOI: 10.1039/c3an01126g
SCHMIDT JESPER MALLING; DAMGAARD HENRIETTE; GREVE-POULSEN MATHIAS; LARSEN LOTTE BACH; HAMMERSHØJ MARIANNE: "Foam and emulsion properties of potato protein isolate and purified fractions", FOOD HYDROCOLLOIDS, ELSEVIER BV, NL, vol. 74, 7 August 2017 (2017-08-07), NL, pages 367 - 378, XP085202090, ISSN: 0268-005X, DOI: 10.1016/j.foodhyd.2017.07.032
Attorney, Agent or Firm:
SUNRAY INTELLECTUAL PROPERTY ATTORNEYS (CN)
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