FUNGAL LIPOLYTIC ENZYMES

WIPO Patent Application WO2005087918

A9

A fungal wild-type lipolytic enzyme having a higher ratio of activity on polar lipids compared with triglycerides, wherein the enzyme preferably has a phospholipidAriglyceride hydrolysing activity ratio of at least 4. Preferably, the lipolytic enzyme according to the present invention has a glycolipid:triglyceride hydrolysing activity ratio of at least 1.5. In one embodiment, the fungal lipolytic enzyme according to the present invention comprises an amino acid sequence as shown in SEQ ID No. 1 or SEQ ID No. 2 or SEQ ID No. 4 or SEQ ID No. 6 or an amino acid sequence which has at least 90% identity thereto. The present invention further encompasses a nucleic acid encoding a fungal lipolytic enzyme, which nucleic acid is selected from the group consisting of: (a) a nucleic acid comprising a nucleotide sequence shown in SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7; (b) a nucleic acid which is related to the nucleotide sequence of SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7 by the degeneration of the genetic code; and (c) nucleic acid comprising a nucleotide sequence which has at least 90% identity with the nucleotide sequence shown in SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7.

BRUNSTEDT JANNE (DK)
MIKKELSEN JOERN DALGAARD (DK)
PEDERSEN HENRIK (DK)
SOEE JOERN BORCH (DK)

PCT/IB2005/000875

August 24, 2006

March 10, 2005

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DANISCO (DK)
BRUNSTEDT JANNE (DK)
MIKKELSEN JOERN DALGAARD (DK)
PEDERSEN HENRIK (DK)
SOEE JOERN BORCH (DK)

C12N9/00; A21D8/04; A23C19/032; A23K1/165; A23K1/18; A23L7/104; A23L7/109; A23L15/10; A23L19/00; A23L23/00; A23L27/00; A23L27/60; A23L29/00; C11B3/00; C12N9/18

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