Title:
FUNGUS STRAIN AND USES THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/002065
Kind Code:
A3
Abstract:
The present invention refers to a Penicillium sumatraense (P. sumatraense) strain comprising in its genome: a) an RNA polymerase II second largest subunit (RPB2) gene sequence having 100%, 99%, or 98% sequence identity with the nucleic acid of SEQ ID NO: 1; b) β-tubulin (BenA) gene sequence having 100%, 99%, or 98% sequence identity with the nucleic acid of SEQ ID NO: 2; c) Calmodulin (CaM) gene sequence having 100%, 99%, or 98% sequence identity with the nucleic acid of SEQ ID NO: 3; and d) an Internal Transcribed Spacer (ITS) sequence having 100%, 99%, or 98% sequence identity with the nucleic acid of SEQ ID NO: 4 and further comprising in its genome, a gene comprising or consisting of a sequence having 100, 99, 98, 97, 96, 95, 94, 93, 92, 91, 90, 89, 88, 87, 86 or 85% sequence identity with SEQ ID NO:30 and/or wherein said strain produces an enzyme that comprises or consists of an amino acid sequence having 100, 99, 98, 97, 96, 95, 94, 93, 92, 91, 90, 89, 88 or 87% sequence identity with SEQ ID NO: 80 or functional fragments comprising the catalytic domain.
Inventors:
BENEDETTI MANUEL (IT)
MATTEI MARIA BENEDETTA (IT)
GIOVANNONI MOIRA (IT)
MATTEI MARIA BENEDETTA (IT)
GIOVANNONI MOIRA (IT)
Application Number:
PCT/EP2022/070846
Publication Date:
March 02, 2023
Filing Date:
July 25, 2022
Export Citation:
Assignee:
UNIV DEGLI STUDI DELLAQUILA (IT)
International Classes:
C12N1/14; C07K14/385; C12N9/14; C12N9/88; C12P21/00
Other References:
MALMSTROM^A J ET AL: "Secondary metabolites characteristic of Penicillium citrinum, Penicillium steckii and related species", PHYTOCHEMISTRY, ELSEVIER, AMSTERDAM , NL, vol. 54, no. 3, 1 June 2000 (2000-06-01), pages 301 - 309, XP004291483, ISSN: 0031-9422, DOI: 10.1016/S0031-9422(00)00106-0
PARK MYUNG SOO ET AL: "New record and enzyme activity of four species inPenicilliumsectionCitrinafrom marine environments in Korea", THE JOURNAL OF MICROBIOLOGY, THE MICROBIOLOGICAL SOCIETY OF KOREA // HAN-GUG MISAENGMUL HAG-HOE, KR, vol. 53, no. 4, 8 April 2015 (2015-04-08), pages 219 - 225, XP035445878, ISSN: 1225-8873, [retrieved on 20150408], DOI: 10.1007/S12275-015-4700-9
PARK MYUNG SOO ET AL: "Diversity and enzyme activity ofPenicilliumspecies associated with macroalgae in Jeju Island", THE JOURNAL OF MICROBIOLOGY, THE MICROBIOLOGICAL SOCIETY OF KOREA // HAN-GUG MISAENGMUL HAG-HOE, KR, vol. 54, no. 10, 30 September 2016 (2016-09-30), pages 646 - 654, XP036067079, ISSN: 1225-8873, [retrieved on 20160930], DOI: 10.1007/S12275-016-6324-0
GIOVANNONI M. ET AL: "A novel Penicillium sumatraense isolate reveals an arsenal of degrading enzymes exploitable in algal bio-refinery processes", vol. 14, no. 1, 1 December 2021 (2021-12-01), pages 180, XP055907387, Retrieved from the Internet DOI: 10.1186/s13068-021-02030-9
RODRIGUES MARCOAURÉLIO ALMENARA ET AL: "Evaluation of Chlorella (Chlorophyta) as Source of Fermentable Sugars via Cell Wall Enzymatic Hydrolysis", vol. 2011, 2 May 2011 (2011-05-02), pages 1 - 5, XP055907886, Retrieved from the Internet DOI: 10.4061/2011/405603
BADER ARACELI NATALIA ET AL: "Efficient saccharification of microalgal biomass by Trichoderma harzianum enzymes for the production of ethanol", ALGAL RESEARCH, vol. 48, 1 June 2020 (2020-06-01), NL, pages 101926, XP055907895, ISSN: 2211-9264, DOI: 10.1016/j.algal.2020.101926
MONJED MOHAMMAD KHALIL ET AL: "Improved saccharification of Chlorella vulgaris biomass by fungal secreted enzymes for bioethanol production", ALGAL RESEARCH, vol. 58, 10 July 2021 (2021-07-10), NL, pages 102402, XP055907902, ISSN: 2211-9264, DOI: 10.1016/j.algal.2021.102402
SAKAMOTO T ET AL: "Purification and properties of two type-B @a-l-arabinofuranosidases produced by Penicillium chrysogenum", BIOCHIMICA ET BIOPHYSICA ACTA, ELSEVIER, AMSTERDAM, NL, vol. 1621, no. 2, 2 May 2003 (2003-05-02), pages 204 - 210, XP004422693, ISSN: 0304-4165, DOI: 10.1016/S0304-4165(03)00058-8
TATSUJI SAKAMOTO ET AL: "Biochemical characterization and gene expression of two endo-arabinanases from31B", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER, BERLIN, DE, vol. 93, no. 3, 12 July 2011 (2011-07-12), pages 1087 - 1096, XP035006091, ISSN: 1432-0614, DOI: 10.1007/S00253-011-3452-7
SILVA LUÍSA DE M B ET AL: "Biochemical Properties of Carbohydrate-Active Enzymes Synthesized byUsing Corn Straw as Carbon Source", WASTE AND BIOMASS VALORIZATION, SPRINGER NETHERLANDS, NL, vol. 11, no. 6, 22 January 2019 (2019-01-22), pages 2455 - 2466, XP037114285, ISSN: 1877-2641, [retrieved on 20190122], DOI: 10.1007/S12649-019-00589-X
PARK MYUNG SOO ET AL: "New record and enzyme activity of four species inPenicilliumsectionCitrinafrom marine environments in Korea", THE JOURNAL OF MICROBIOLOGY, THE MICROBIOLOGICAL SOCIETY OF KOREA // HAN-GUG MISAENGMUL HAG-HOE, KR, vol. 53, no. 4, 8 April 2015 (2015-04-08), pages 219 - 225, XP035445878, ISSN: 1225-8873, [retrieved on 20150408], DOI: 10.1007/S12275-015-4700-9
PARK MYUNG SOO ET AL: "Diversity and enzyme activity ofPenicilliumspecies associated with macroalgae in Jeju Island", THE JOURNAL OF MICROBIOLOGY, THE MICROBIOLOGICAL SOCIETY OF KOREA // HAN-GUG MISAENGMUL HAG-HOE, KR, vol. 54, no. 10, 30 September 2016 (2016-09-30), pages 646 - 654, XP036067079, ISSN: 1225-8873, [retrieved on 20160930], DOI: 10.1007/S12275-016-6324-0
GIOVANNONI M. ET AL: "A novel Penicillium sumatraense isolate reveals an arsenal of degrading enzymes exploitable in algal bio-refinery processes", vol. 14, no. 1, 1 December 2021 (2021-12-01), pages 180, XP055907387, Retrieved from the Internet
RODRIGUES MARCOAURÉLIO ALMENARA ET AL: "Evaluation of Chlorella (Chlorophyta) as Source of Fermentable Sugars via Cell Wall Enzymatic Hydrolysis", vol. 2011, 2 May 2011 (2011-05-02), pages 1 - 5, XP055907886, Retrieved from the Internet
BADER ARACELI NATALIA ET AL: "Efficient saccharification of microalgal biomass by Trichoderma harzianum enzymes for the production of ethanol", ALGAL RESEARCH, vol. 48, 1 June 2020 (2020-06-01), NL, pages 101926, XP055907895, ISSN: 2211-9264, DOI: 10.1016/j.algal.2020.101926
MONJED MOHAMMAD KHALIL ET AL: "Improved saccharification of Chlorella vulgaris biomass by fungal secreted enzymes for bioethanol production", ALGAL RESEARCH, vol. 58, 10 July 2021 (2021-07-10), NL, pages 102402, XP055907902, ISSN: 2211-9264, DOI: 10.1016/j.algal.2021.102402
SAKAMOTO T ET AL: "Purification and properties of two type-B @a-l-arabinofuranosidases produced by Penicillium chrysogenum", BIOCHIMICA ET BIOPHYSICA ACTA, ELSEVIER, AMSTERDAM, NL, vol. 1621, no. 2, 2 May 2003 (2003-05-02), pages 204 - 210, XP004422693, ISSN: 0304-4165, DOI: 10.1016/S0304-4165(03)00058-8
TATSUJI SAKAMOTO ET AL: "Biochemical characterization and gene expression of two endo-arabinanases from31B", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER, BERLIN, DE, vol. 93, no. 3, 12 July 2011 (2011-07-12), pages 1087 - 1096, XP035006091, ISSN: 1432-0614, DOI: 10.1007/S00253-011-3452-7
SILVA LUÍSA DE M B ET AL: "Biochemical Properties of Carbohydrate-Active Enzymes Synthesized byUsing Corn Straw as Carbon Source", WASTE AND BIOMASS VALORIZATION, SPRINGER NETHERLANDS, NL, vol. 11, no. 6, 22 January 2019 (2019-01-22), pages 2455 - 2466, XP037114285, ISSN: 1877-2641, [retrieved on 20190122], DOI: 10.1007/S12649-019-00589-X
Attorney, Agent or Firm:
TURRI, Elisa et al. (IT)
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