Title:
FUSION PROTEIN BASED ON SINGLE-CHAIN ANTIBODY FRAGMENT, NANO-ASSEMBLY, AND PREPARATION METHODS THEREFOR AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/166832
Kind Code:
A1
Abstract:
A recombinant fusion protein, a nano-assembly, and preparation methods therefor and the use thereof. The nano-assembly is constructed from at least one recombinant fusion protein and a hydrophobic degradable polyester and a derivative thereof. The recombinant fusion protein comprises a single-chain antibody fragment, a linker peptide and an albumin having a hydrophobic region, wherein the single-chain antibody fragment has at least one of the effects of competitively binding to a target, inhibiting a signaling pathway, activating a signaling pathway, and targeting an antigen. The present invention further relates to a method for preparing a monoclonal antibody fragment-albumin recombinant fusion protein, and a method for constructing a nano-assembly. On the basis of the nano-assembly, a brand-new method for constructing a mono-specific, bispecific or multi-specific antibody is provided, and the nano-assembly can be used for treating diseases such as tumors and autoimmune diseases.
Inventors:
WANG JUN (CN)
SHEN SONG (CN)
ZHAO DONGKUN (CN)
SHEN SONG (CN)
ZHAO DONGKUN (CN)
Application Number:
PCT/CN2022/074716
Publication Date:
August 11, 2022
Filing Date:
January 28, 2022
Export Citation:
Assignee:
UNIV SOUTH CHINA TECH (CN)
International Classes:
A61K39/395; A61K47/62; A61K47/69; A61P35/00; A61P37/00
Domestic Patent References:
| WO2011095545A1 | 2011-08-11 |
Foreign References:
| US20160120991A1 | 2016-05-05 | |||
| US20130136775A1 | 2013-05-30 |
Other References:
V. BERGER, F. RICHTER, K. ZETTLITZ, F. UNVERDORBEN, P. SCHEURICH, A. HERRMANN, K. PFIZENMAIER, R. E. KONTERMANN: "An anti-TNFR1 scFv-HSA fusion protein as selective antagonist of TNF action", PROTEIN ENGINEERING, DESIGN AND SELECTION, OXFORD JOURNAL, vol. 26, no. 10, 1 October 2013 (2013-10-01), GB , pages 581 - 587, XP055279872, ISSN: 1741-0126, DOI: 10.1093/protein/gzt044
HOU-BING ZHANG;TAI-YU TIAN;QI FENG;XIN-YU HE;XIAO-JIAO DU;HUA DONG;MENG-HUA XIONG;LI-FENG YAN;JUN WANG: "Microfluidic Preparation of Microparticles Capable of Sustained Release of Nanoparticles", ACTA POLYMERICA SINICA, vol. 51, no. 11, 3 July 2020 (2020-07-03), pages 1240 - 1247, XP055957153, ISSN: 1000-3304, DOI: 10.11777/j.issn1000-3304.2020.20090
WANG JIAN-ZHU;BI YAN-PING;LI FEI;CHEN YING: "Preparation of Bovine Serum Albumin-Modified PLGA Nanoparticles Loaded With Naringenin", CHINESE TRADITIONAL PATENT MEDICINE, vol. 41, no. 11, 1 November 2019 (2019-11-01), pages 2566 - 2571, XP055955551, ISSN: 1001-1528, DOI: 10.3969/j.issn.1001-1528.2019.11.003
HOU-BING ZHANG;TAI-YU TIAN;QI FENG;XIN-YU HE;XIAO-JIAO DU;HUA DONG;MENG-HUA XIONG;LI-FENG YAN;JUN WANG: "Microfluidic Preparation of Microparticles Capable of Sustained Release of Nanoparticles", ACTA POLYMERICA SINICA, vol. 51, no. 11, 3 July 2020 (2020-07-03), pages 1240 - 1247, XP055957153, ISSN: 1000-3304, DOI: 10.11777/j.issn1000-3304.2020.20090
WANG JIAN-ZHU;BI YAN-PING;LI FEI;CHEN YING: "Preparation of Bovine Serum Albumin-Modified PLGA Nanoparticles Loaded With Naringenin", CHINESE TRADITIONAL PATENT MEDICINE, vol. 41, no. 11, 1 November 2019 (2019-11-01), pages 2566 - 2571, XP055955551, ISSN: 1001-1528, DOI: 10.3969/j.issn.1001-1528.2019.11.003
Attorney, Agent or Firm:
GUANGZHOU PRIME IP LAW OFFICE (CN)
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