Title:
FUSION PROTEIN CONTAINING CAS PROTEIN AND BACTERIAL TOXIN AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2024/085674
Kind Code:
A1
Abstract:
The present invention relates to a fusion protein containing a CAS protein and a bacterial toxin, and a use thereof. A fusion protein, a polypeptide thereof, and a CRISPR-Cas system including same according to the present invention allow for effective base editing. In addition, because of its small size, the polypeptide facilitates easy delivery through vectors. Furthermore, when indels are induced therethrough, indel efficiency significantly increases, and for indel induction, the size of nucleotides where indels are formed also increases, whereby effective utilization can be made for gene knockout.
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Inventors:
KIM YONG SUB (KR)
KWON JI YEON (KR)
KIM DAE SIK (KR)
KWON JI YEON (KR)
KIM DAE SIK (KR)
Application Number:
PCT/KR2023/016230
Publication Date:
April 25, 2024
Filing Date:
October 19, 2023
Export Citation:
Assignee:
ASAN FOUND (KR)
UNIV ULSAN FOUND IND COOP (KR)
RESEARCH＆BUSINESS FOUNDATION SUNGKYUNKWAN UNIV (KR)
UNIV ULSAN FOUND IND COOP (KR)
RESEARCH＆BUSINESS FOUNDATION SUNGKYUNKWAN UNIV (KR)
International Classes:
C12N9/22; C12N9/78; C12N15/10; C12N15/113; C12N15/62
Domestic Patent References:
| WO2022212584A1 | 2022-10-06 | |||
| WO2021048272A1 | 2021-03-18 |
Foreign References:
| KR20190008548A | 2019-01-24 | |||
| KR20210005184A | 2021-01-13 |
Other References:
ELENA VELASQUEZ MUNOZ: "Recombination-independent genomic editing and chromosomal site-focused diversification of Gram-negative bacteria", DOCTORAL THESIS, UNIVERSIDAD AUTONOMA DE MADRID, 1 January 2021 (2021-01-01), XP093161794, Retrieved from the Internet
MOK BEVERLY Y.; DE MORAES MARCOS H.; ZENG JUN; BOSCH DUSTIN E.; KOTRYS ANNA V.; RAGURAM ADITYA; HSU FOSHENG; RADEY MATTHEW C.; PET: "A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing", NATURE, vol. 583, no. 7817, 8 July 2020 (2020-07-08), pages 631 - 637, XP037200062, DOI: 10.1038/s41586-020-2477-4
MOK BEVERLY Y.; DE MORAES MARCOS H.; ZENG JUN; BOSCH DUSTIN E.; KOTRYS ANNA V.; RAGURAM ADITYA; HSU FOSHENG; RADEY MATTHEW C.; PET: "A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing", NATURE, vol. 583, no. 7817, 8 July 2020 (2020-07-08), pages 631 - 637, XP037200062, DOI: 10.1038/s41586-020-2477-4
Attorney, Agent or Firm:
THEWAVE IP LAW FIRM (KR)
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