Title:
FUSION PROTEIN CONTAINING ERYTHROPOIETIN POLYPEPTIDE
Document Type and Number:
WIPO Patent Application WO/2021/251438
Kind Code:
A1
Abstract:
The present invention relates to a fusion protein including an erythropoietin polypeptide fused to the Fc region of IgG derived from a mammal (elsewhere also referred to as an EPO-Fc fusion protein). The erythropoietin polypeptide has an amino acid sequence represented by SEQ ID NO: 9 or 10. The Fc region has an amino acid sequence represented by SEQ ID NO: 3 or 4. The fusion protein has superior physical properties and activity and can be acquired at a high yield rate/high yield.
Inventors:
NAKAO RYOTA (JP)
IZUMI YOSHIKATSU (JP)
HATTORI KUNIHIRO (JP)
IZUMI YOSHIKATSU (JP)
HATTORI KUNIHIRO (JP)
Application Number:
PCT/JP2021/021972
Publication Date:
December 16, 2021
Filing Date:
June 09, 2021
Export Citation:
Assignee:
BICA THERAPEUTICS INC (JP)
International Classes:
C07K19/00; A61K38/22; A61K47/68; A61P7/06; C12N15/12; C12N15/13; C12N15/62; C12P21/02
Domestic Patent References:
| WO2018226747A1 | 2018-12-13 | |||
| WO2018073185A1 | 2018-04-26 | |||
| WO2020116560A1 | 2020-06-11 | |||
| WO2000042072A2 | 2000-07-20 | |||
| WO2002060919A2 | 2002-08-08 | |||
| WO2006020114A2 | 2006-02-23 | |||
| WO2006031370A2 | 2006-03-23 | |||
| WO2010033279A2 | 2010-03-25 | |||
| WO2006053301A2 | 2006-05-18 | |||
| WO2009086320A1 | 2009-07-09 |
Foreign References:
| JP2008504008A | 2008-02-14 | |||
| JP2009524415A | 2009-07-02 | |||
| EP2154157A2 | 2010-02-17 | |||
| US20070141052A1 | 2007-06-21 | |||
| JP2020101237A | 2020-07-02 |
Other References:
RANDOLPH JOHN F; SCARLETT JANET M; STOKOL TRACY; SAUNDERS KATHRYN M; MACLEOD JAMES N: "Expression, bioactivity, and clinical assessment of recombinant feline erythropoietin", AMERICAN JOURNAL OF VETERINARY RESEARCH, AMERICAN VETERINARY MEDICINE ASSOCIATION, US, vol. 65, no. 10, 1 October 2004 (2004-10-01), US , pages 1355 - 1366, XP008129220, ISSN: 0002-9645
WEN D., ET AL.: "ERYTHROPOEITIN STRUCTURE-FUNCTION RELATIONSHIPS: HIGH DEGREE OF SEQUENCE HOMOLOGY AMONG MAMMALS.", BLOOD, AMERICAN SOCIETY OF HEMATOLOGY, US, vol. 82., no. 05., 1 September 1993 (1993-09-01), US , pages 1507 - 1516., XP000938929, ISSN: 0006-4971
YEUNG YA ET AL., J. IMMUNOL., vol. 182, 2009, pages 7663 - 71
DATTA-MANNAN A ET AL., J. BIOL. CHEM., vol. 282, 2007, pages 1709 - 17
DALL'ACQUA WF ET AL., J. IMMUNOL., vol. 169, 2002, pages 5171 - 80
TANG ET AL., VET. IMMUNOL. IMMUNOPATHOL., vol. 80, no. 3-4, 2001, pages 259 - 270
KANAI, T.H. ET AL.: "Identification of two allelic IgG1 C(H)coding regions (Cgammal) of cat", VET. IMMUNOL. IMMUNOPATHOL., vol. 73, no. 1, 2000, pages 53 - 62
STRIETZEL, C.J. ET AL.: "In Vitro functional characterization of feline IgGs", VET. IMMUNOL. IMMUNOPATHOL., vol. 158, no. 3-4, 2014, pages 214 - 233
GHETIE ET AL., IMMUNOL. TODAY, vol. 18, no. 12, 1997, pages 592 - 598
RAGHAVAN ET AL., IMMUNITY, vol. 1, 1994, pages 303 - 315
KABAT ET AL.: "Sequences of Proteins of Immunological Interest", 1991, NATIONAL INSTITUTES OF HEALTH
CURRENT PHARMACEUTICAL BIOTECHNOLOGY, vol. 17, 2016, pages 1298 - 1314
PROC NATL ACSD SCI USA, vol. 81, 1984, pages 5662 - 5666
SAMBROOK ET AL.: "Molecular Cloning A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
ANNU. REV. BIOPHYS. BIOMOL. STRUCT., vol. 35, 2006, pages 225 - 249
PROC. NATL. ACAD. SCI. U.S.A., vol. 100, no. 11, 2003, pages 6353 - 6357
DRUG METAB DISPOS, vol. 35, no. 1, January 2007 (2007-01-01), pages 86 - 94
INT IMMUNOL, vol. 18, no. 12, December 2006 (2006-12-01), pages 1759 - 69
J BIOL CHEM, vol. 276, no. 9, 2 March 2001 (2001-03-02), pages 6591 - 604
J BIOL CHEM, vol. 282, no. 3, 2007, pages 1709 - 17
J IMMUNOL, vol. 169, no. 9, 2002, pages 5171 - 80
J IMMUNOL, vol. 182, no. 12, 2009, pages 7663 - 71
MOLECULAR CELL, vol. 7, April 2001 (2001-04-01), pages 867 - 877
NAT BIOTECHNOL, vol. 15, no. 7, July 1997 (1997-07-01), pages 637 - 40
NAT BIOTECHNOL, vol. 23, no. 10, October 2005 (2005-10-01), pages 1283 - 8
PROC NATL ACAD SCI U S A., vol. 103, no. 49, 5 December 2006 (2006-12-05), pages 18709 - 14
"GenBank", Database accession no. XP-023100998.1
MOL CELL BIOL, vol. 8, 1988, pages 466 - 472
J. EXP. MED., vol. 108, 1995, pages 94
VALLE ET AL., NATURE, vol. 291, 1981, pages 338 - 340
PACE ET AL., PROTEIN SCIENCE, vol. 4, 1995, pages 2411 - 2423
WEN D., ET AL.: "ERYTHROPOEITIN STRUCTURE-FUNCTION RELATIONSHIPS: HIGH DEGREE OF SEQUENCE HOMOLOGY AMONG MAMMALS.", BLOOD, AMERICAN SOCIETY OF HEMATOLOGY, US, vol. 82., no. 05., 1 September 1993 (1993-09-01), US , pages 1507 - 1516., XP000938929, ISSN: 0006-4971
YEUNG YA ET AL., J. IMMUNOL., vol. 182, 2009, pages 7663 - 71
DATTA-MANNAN A ET AL., J. BIOL. CHEM., vol. 282, 2007, pages 1709 - 17
DALL'ACQUA WF ET AL., J. IMMUNOL., vol. 169, 2002, pages 5171 - 80
TANG ET AL., VET. IMMUNOL. IMMUNOPATHOL., vol. 80, no. 3-4, 2001, pages 259 - 270
KANAI, T.H. ET AL.: "Identification of two allelic IgG1 C(H)coding regions (Cgammal) of cat", VET. IMMUNOL. IMMUNOPATHOL., vol. 73, no. 1, 2000, pages 53 - 62
STRIETZEL, C.J. ET AL.: "In Vitro functional characterization of feline IgGs", VET. IMMUNOL. IMMUNOPATHOL., vol. 158, no. 3-4, 2014, pages 214 - 233
GHETIE ET AL., IMMUNOL. TODAY, vol. 18, no. 12, 1997, pages 592 - 598
RAGHAVAN ET AL., IMMUNITY, vol. 1, 1994, pages 303 - 315
KABAT ET AL.: "Sequences of Proteins of Immunological Interest", 1991, NATIONAL INSTITUTES OF HEALTH
CURRENT PHARMACEUTICAL BIOTECHNOLOGY, vol. 17, 2016, pages 1298 - 1314
PROC NATL ACSD SCI USA, vol. 81, 1984, pages 5662 - 5666
SAMBROOK ET AL.: "Molecular Cloning A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
ANNU. REV. BIOPHYS. BIOMOL. STRUCT., vol. 35, 2006, pages 225 - 249
PROC. NATL. ACAD. SCI. U.S.A., vol. 100, no. 11, 2003, pages 6353 - 6357
DRUG METAB DISPOS, vol. 35, no. 1, January 2007 (2007-01-01), pages 86 - 94
INT IMMUNOL, vol. 18, no. 12, December 2006 (2006-12-01), pages 1759 - 69
J BIOL CHEM, vol. 276, no. 9, 2 March 2001 (2001-03-02), pages 6591 - 604
J BIOL CHEM, vol. 282, no. 3, 2007, pages 1709 - 17
J IMMUNOL, vol. 169, no. 9, 2002, pages 5171 - 80
J IMMUNOL, vol. 182, no. 12, 2009, pages 7663 - 71
MOLECULAR CELL, vol. 7, April 2001 (2001-04-01), pages 867 - 877
NAT BIOTECHNOL, vol. 15, no. 7, July 1997 (1997-07-01), pages 637 - 40
NAT BIOTECHNOL, vol. 23, no. 10, October 2005 (2005-10-01), pages 1283 - 8
PROC NATL ACAD SCI U S A., vol. 103, no. 49, 5 December 2006 (2006-12-05), pages 18709 - 14
"GenBank", Database accession no. XP-023100998.1
MOL CELL BIOL, vol. 8, 1988, pages 466 - 472
J. EXP. MED., vol. 108, 1995, pages 94
VALLE ET AL., NATURE, vol. 291, 1981, pages 338 - 340
PACE ET AL., PROTEIN SCIENCE, vol. 4, 1995, pages 2411 - 2423
Attorney, Agent or Firm:
TAKASHIMA, Hajime (JP)
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