Title:
FUSION PROTEIN OF Z-DOMAIN AND CALSEQUESTRIN, HAVING IMPROVED REACTIVITY, STABILITY, AND ANTIBODY RECOVERY, AND METHOD FOR ISOLATION AND PURIFICATION OF ANTIBODY USING SAME
Document Type and Number:
WIPO Patent Application WO/2021/201405
Kind Code:
A1
Abstract:
The present invention relates to a fusion protein of the Z-domain and calsequestrin, having improved reactivity, stability, and antibody recovery, and a method for isolation and purification of an antibody using same. Specifically, the present invention relates to: a nucleic acid encoding a fusion protein of the Z-domain and calsequestrin having improved reactivity and stability; a recombinant expression vector comprising the nucleic acid; a transformant transformed with the recombinant expression vector; and a method for isolation and purification of an antibody with improved reactivity, stability, antibody recovery, and purity using the fusion protein of the Z-domain and calsequestrin.
Inventors:
KIM SUNG HYUN (KR)
PARK HEE SUN (KR)
PARK HEE SUN (KR)
Application Number:
PCT/KR2021/001233
Publication Date:
October 07, 2021
Filing Date:
January 29, 2021
Export Citation:
Assignee:
KOREA INST CERAMIC ENG & TECH (KR)
International Classes:
C12N15/62; C07K1/22; C07K14/00; C07K16/00; C07K19/00; C12N15/70
Domestic Patent References:
| WO1997034631A1 | 1997-09-25 | |||
| WO1996032478A1 | 1996-10-17 |
Foreign References:
| KR20170069452A | 2017-06-21 | |||
| US20060141570A1 | 2006-06-29 | |||
| KR20140092227A | 2014-07-23 | |||
| KR101774354B1 | 2017-09-04 |
Other References:
DATABASE Protein 18 November 1997 (1997-11-18), ANONYMOUS: "cardiac calsequestrin [Homo sapiens]", XP055854630, retrieved from Genbank Database accession no. BAA23494
E. WAHLBERG, C. LENDEL, M. HELGSTRAND, P. ALLARD, V. DINCBAS-RENQVIST, A. HEDQVIST, H. BERGLUND, P.-A. NYGREN, T. HARD: "An affibody in complex with a target protein: Structure and coupled folding", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 100, no. 6, 18 March 2003 (2003-03-18), pages 3185 - 3190, XP055102112, ISSN: 0027-8424, DOI: 10.1073/pnas.0436086100
DATABASE Protein 23 March 2015 (2015-03-23), ANONYMOUS: "calsequestrin 1 (fast-twitch, skeletal muscle) [Homo sapiens]", XP055854638, retrieved from Genbank Database accession no. EAW52736
H. NEURATHR.L. HILL: "The Proteins", 1979, ACADEMIC PRESS
E. WAHLBERG, C. LENDEL, M. HELGSTRAND, P. ALLARD, V. DINCBAS-RENQVIST, A. HEDQVIST, H. BERGLUND, P.-A. NYGREN, T. HARD: "An affibody in complex with a target protein: Structure and coupled folding", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 100, no. 6, 18 March 2003 (2003-03-18), pages 3185 - 3190, XP055102112, ISSN: 0027-8424, DOI: 10.1073/pnas.0436086100
DATABASE Protein 23 March 2015 (2015-03-23), ANONYMOUS: "calsequestrin 1 (fast-twitch, skeletal muscle) [Homo sapiens]", XP055854638, retrieved from Genbank Database accession no. EAW52736
H. NEURATHR.L. HILL: "The Proteins", 1979, ACADEMIC PRESS
Attorney, Agent or Firm:
WETHEPEOPLE IP & LAW FIRM (KR)
Download PDF: