Title:
GAP JUNCTION PERMEABILITY ASSAY
Document Type and Number:
WIPO Patent Application WO2002063298
Kind Code:
A3
Abstract:
The present invention is a gap junction assay method and provides methods for both characterizing connexins and for identifying compounds that affect gap junctions function.
Inventors:
BARBIER ANN J (US)
BARON BRUCE M (US)
BARON BRUCE M (US)
Application Number:
PCT/US2002/002954
Publication Date:
February 19, 2004
Filing Date:
January 31, 2002
Export Citation:
Assignee:
AVENTIS PHARMA INC (US)
BARBIER ANN J (US)
BARON BRUCE M (US)
BARBIER ANN J (US)
BARON BRUCE M (US)
International Classes:
G01N33/48; C12N15/09; C12Q1/02; C12Q1/68; G01N33/50; G01N33/566; (IPC1-7): G01N33/50; G01N33/566
Domestic Patent References:
| WO2000013651A2 | 2000-03-16 | |||
| WO1999026584A2 | 1999-06-03 |
Other References:
BARBIER ANN J ET AL: "Development of a prototype reporter-based assay for the evaluation of gap junctional intercellular communication.", BIOTECHNOLOGY LETTERS, vol. 23, no. 19, October 2001 (2001-10-01), pages 1559 - 1563, XP009005715, ISSN: 0141-5492
ANDRADE-ROZENTAL A F ET AL: "Gap junctions: The "kiss of death" and the "kiss of life".", BRAIN RESEARCH REVIEWS, vol. 32, no. 1, April 2000 (2000-04-01), pages 308 - 315, XP002231074, ISSN: 0165-0173
SANDBERG K ET AL: "ANGIOTENSIN II-INDUCED CALCIUM MOBILIZATION IN OOCYTES BY SIGNAL TRANSFER THROUGH GAP JUNCTIONS", SCIENCE (WASHINGTON D C), vol. 249, no. 4966, 1990, pages 298 - 301, XP001097272, ISSN: 0036-8075
MURRAY S A ET AL: "HORMONE INDUCED INTER CELLULAR SIGNAL TRANSFER DISSOCIATES CYCLIC AMP DEPENDENT PROTEIN KINASE", JOURNAL OF CELL BIOLOGY, vol. 98, no. 5, 1984, pages 1710 - 1719, XP009005845, ISSN: 0021-9525
KUMAR NALIN M ET AL: "The gap junction communication channel.", CELL, vol. 84, no. 3, 1996, pages 381 - 388, XP002231075, ISSN: 0092-8674
GEORGE C H ET AL: "Rapid determination of gap junction formation using HeLa cells microinjected with cDNAs encoding wild-type and chimeric connexins.", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. UNITED STATES 29 JUN 1998, vol. 247, no. 3, 29 June 1998 (1998-06-29), pages 785 - 789, XP002231076, ISSN: 0006-291X
ANDRADE-ROZENTAL A F ET AL: "Gap junctions: The "kiss of death" and the "kiss of life".", BRAIN RESEARCH REVIEWS, vol. 32, no. 1, April 2000 (2000-04-01), pages 308 - 315, XP002231074, ISSN: 0165-0173
SANDBERG K ET AL: "ANGIOTENSIN II-INDUCED CALCIUM MOBILIZATION IN OOCYTES BY SIGNAL TRANSFER THROUGH GAP JUNCTIONS", SCIENCE (WASHINGTON D C), vol. 249, no. 4966, 1990, pages 298 - 301, XP001097272, ISSN: 0036-8075
MURRAY S A ET AL: "HORMONE INDUCED INTER CELLULAR SIGNAL TRANSFER DISSOCIATES CYCLIC AMP DEPENDENT PROTEIN KINASE", JOURNAL OF CELL BIOLOGY, vol. 98, no. 5, 1984, pages 1710 - 1719, XP009005845, ISSN: 0021-9525
KUMAR NALIN M ET AL: "The gap junction communication channel.", CELL, vol. 84, no. 3, 1996, pages 381 - 388, XP002231075, ISSN: 0092-8674
GEORGE C H ET AL: "Rapid determination of gap junction formation using HeLa cells microinjected with cDNAs encoding wild-type and chimeric connexins.", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. UNITED STATES 29 JUN 1998, vol. 247, no. 3, 29 June 1998 (1998-06-29), pages 785 - 789, XP002231076, ISSN: 0006-291X
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