Title:
GENE ANALYSIS METHOD, GENE ANALYSIS APPARATUS, AND GENE ANALYSIS KIT
Document Type and Number:
WIPO Patent Application WO/2023/243147
Kind Code:
A1
Abstract:
The present invention relates to: a gene analysis method for performing the quantitative analysis of a gene mutation using a single-base extension reaction; and a gene analysis apparatus and a gene analysis kit which are based on said method. Specifically, the present invention provides a gene analysis method comprising: a step for performing a single-base extension reaction by using a single-base extension reaction primer for detecting a target base sequence, and a single-base extension reaction substrate having a fluorescent dye; a step for subjecting the reaction product of the single-base extension reaction to electrophoresis; and a step for measuring the mobility of the electrophoresis and the fluorescence intensity of the fluorescent dye to quantify the content ratio of a plurality of target base sequences on the basis of the magnitude of the fluorescence intensity, the gene analysis method being characterized in that a single-base extension reaction substrate having no fluorescent dye is mixed in the single-base extension reaction, and the mixing ratio between the substrate having no fluorescent dye and the substrate having the fluorescent dye is (a) set according to the ratio of the excitation efficiencies of fluorescent dyes to be detected when at least two fluorescent dyes are used, and/or (b) set according to the efficiency of binding and incorporating the substrate into the primer to be used when at least two primers are used.
Inventors:
ANDO TAKAHIRO (JP)
YOKOI TAKAHIDE (JP)
MANRI CHIHIRO (JP)
ANAZAWA TAKASHI (JP)
KAWAKAMI SHOKO (JP)
ISHIDA TAKESHI (JP)
YOKOI TAKAHIDE (JP)
MANRI CHIHIRO (JP)
ANAZAWA TAKASHI (JP)
KAWAKAMI SHOKO (JP)
ISHIDA TAKESHI (JP)
Application Number:
PCT/JP2023/006013
Publication Date:
December 21, 2023
Filing Date:
February 20, 2023
Export Citation:
Assignee:
HITACHI LTD (JP)
International Classes:
C12Q1/6876; C12M1/00; C12Q1/6851
Domestic Patent References:
| WO2014061146A1 | 2014-04-24 |
Foreign References:
| JP2006508632A | 2006-03-16 | |||
| JP2006521086A | 2006-09-21 | |||
| JP2004526423A | 2004-09-02 | |||
| JP2003310300A | 2003-11-05 | |||
| CN1982470A | 2007-06-20 | |||
| JP2016189704A | 2016-11-10 |
Other References:
FUKUSHIMA HIROFUMI: "Use of Florescence-labelled primers in STR multi-type analysis", JPN. J. ELECTROPH, vol. 41, no. 6, 1 January 1997 (1997-01-01), pages 301 - 305, XP093117039
Attorney, Agent or Firm:
HIRAKI & ASSOCIATES (JP)
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