Title:
GENE CONSTRUCT FOR PRODRUG ACTIVATION ENCODING A HETEROLOGOUS, GLYCOSYLPHOPHATI DYLINOSITOL-MODIFIED CARBOYPEPTIDASE G2 AND A CELL SURFACE TARGETTNG SIGNAL PEPTIDE
Document Type and Number:
WIPO Patent Application WO2000066752
Kind Code:
A3
Abstract:
This invention relates particularly to gene directed enzyme prodrug therapy (GDEPT) using post translational glycosylphosphatildylinositol (GPI) addition to a prodrug activating enzyme to enable anchorage of the enzyme at the cell surface, particularly for use in cancer therapy. A preferred prodrug activating enzyme is carboxypeptidase G2.
Inventors:
CASTRO MARIA GRACIELA (GB)
EMERY STEPHEN CHARLES (GB)
LOWENSTEIN PEDRO RICARDO (GB)
EMERY STEPHEN CHARLES (GB)
LOWENSTEIN PEDRO RICARDO (GB)
Application Number:
PCT/GB2000/001640
Publication Date:
April 05, 2001
Filing Date:
April 28, 2000
Export Citation:
Assignee:
ASTRAZENECA AB (SE)
UNIV MANCHESTER (GB)
CASTRO MARIA GRACIELA (GB)
EMERY STEPHEN CHARLES (GB)
LOWENSTEIN PEDRO RICARDO (GB)
UNIV MANCHESTER (GB)
CASTRO MARIA GRACIELA (GB)
EMERY STEPHEN CHARLES (GB)
LOWENSTEIN PEDRO RICARDO (GB)
International Classes:
A61K31/198; A61K31/222; A61K35/76; A61K38/00; C12N15/09; A61K38/44; A61K38/46; A61K48/00; A61P35/00; A61P43/00; C07K14/705; C12N9/48; C12N15/57; C12N15/62; (IPC1-7): C12N15/57; C12N9/48; A61K47/48; A61K48/00
Domestic Patent References:
| WO1997019180A2 | 1997-05-29 | |||
| WO1998051787A2 | 1998-11-19 |
Other References:
HAMSTRA, D.A. ET AL.: "Expression of Endogenously Activated Secreted or Cell Surface Carboxypeptidase A Sensitizes Tumor Cells to Methotrexate-alpha-Peptide Prodrugs", CANCER RESEARCH, vol. 60, no. 3, 1 February 2000 (2000-02-01), pages 657 - 665, XP000926076
SEKI, T. ET AL.: "The human Thy-1 gene: Structure and chromosomal location", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 82, no. 19, October 1985 (1985-10-01), pages 6657 - 6661, XP000926079
MARAIS, R. ET AL.: "A cell surface tethered enzyme improves efficiency in gene-directed enzyme prodrug therapy", NATURE BIOTECHNOLOGY, vol. 15, no. 13, December 1997 (1997-12-01), pages 1373 - 1377, XP002079969
SEKI, T. ET AL.: "The human Thy-1 gene: Structure and chromosomal location", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 82, no. 19, October 1985 (1985-10-01), pages 6657 - 6661, XP000926079
MARAIS, R. ET AL.: "A cell surface tethered enzyme improves efficiency in gene-directed enzyme prodrug therapy", NATURE BIOTECHNOLOGY, vol. 15, no. 13, December 1997 (1997-12-01), pages 1373 - 1377, XP002079969
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