Title:
GENE TARGETING VECTOR, AND METHOD FOR USING SAME
Document Type and Number:
WIPO Patent Application WO/2013/089123
Kind Code:
A1
Abstract:
Provided is a gene targeting vector which enables highly efficient gene targeting. A gene targeting vector having a structure which sandwiches a positive selection marker between homologous DNA in the 5' upstream region of a target site and homologous DNA in the 3' downstream region of the target site, wherein a DNA sequence enabling bicistronic expression and a splice acceptor site are added 5' upstream of the positive selection marker, and a splice acceptor site is also added 5' upstream of the homologous DNA in the 5' upstream region of the target site.
Inventors:
ADACHI NORITAKA (JP)
Application Number:
PCT/JP2012/082160
Publication Date:
June 20, 2013
Filing Date:
December 12, 2012
Export Citation:
Assignee:
PUBLIC UNIV CORP YOKOHAMA CITY (JP)
International Classes:
C12N15/09; C12N1/15; C12N1/19; C12N1/21; C12N5/10
Foreign References:
| JPH09500005A | 1997-01-07 | |||
| US5922601A | 1999-07-13 | |||
| JP2011118564A | 2011-05-27 |
Other References:
CAPECCHI, MR: "Altering the genome by homologous recombination", SCIENCE, vol. 244, 1989, pages 1288 - 1292
VASQUEZ KM; MARBURGER K; INTODY Z ET AL.: "Manipulating the mammalian genome by homologous recombination", PROC. NATL. ACAD. SCI. USA, vol. 98, 2001, pages 8403 - 8410
YANEZ RJ; PORTER AC: "Therapeutic gene targeting", GENE THER, vol. 5, 1998, pages 149 - 159
BUNZ F; DUTRIAUX A; LENGAUER C ET AL.: "Requirement for p53 and p21 to Sustain G2 Arrest After DNA Damage", SCIENCE, vol. 282, 1998, pages 1497 - 1501
ADACHI N; SO S; IIIZUMI S ET AL.: "The human pre-B cell line Nalm-6 is highly proficient in gene targeting by homologous recombination", DNA CELL BIOL., vol. 25, 2006, pages 19 - 24
IIIZUMI, S; NOMURA, Y; SO, S ET AL.: "Simple one-week method to construct gene-targeting vectors: application to production of human knockout cell lines", BIOTECHNIQUES, vol. 41, 2006, pages 311 - 316
ADACHI, N; SO, S; IIIZUMI, S ET AL.: "The human pre-B cell line Nalm-6 is highly proficient in gene targeting by homologous recombination", DNA CELL BIOL., vol. 25, 2006, pages 19 - 24
ADACHI, N; NISHIJIMA, H; SHIBAHARA, K: "Gene targeting using the human Nalm-6 pre-B cell line", BIOSCIENCE TRENDS., vol. 2, 2008, pages 169 - 180
TOYODA, E; KAGAYA, S; COWELL, IG ET AL.: "NK314, a topoisomerase II inhibitor that specifically targets the alpha isoform", J. BIOL. CHEM., vol. 283, 2008, pages 23711 - 23720
IIIZUMI, S; NOMURA, Y; SO, S ET AL.: "Simple one-week method to construct gene- targeting vectors: application to production of human knockout cell lines", BIOTECHNIQUES, vol. 41, 2006, pages 311 - 316
FRIEDRICH, G.; SORIANO P, GENES DEV., vol. 5, 1991, pages 1513 - 1523
IIIZUMI ET AL., NUCLEIC ACIDS RES., vol. 36, no. 19, November 2008 (2008-11-01), pages 6333 - 6342
See also references of EP 2792744A4
VASQUEZ KM; MARBURGER K; INTODY Z ET AL.: "Manipulating the mammalian genome by homologous recombination", PROC. NATL. ACAD. SCI. USA, vol. 98, 2001, pages 8403 - 8410
YANEZ RJ; PORTER AC: "Therapeutic gene targeting", GENE THER, vol. 5, 1998, pages 149 - 159
BUNZ F; DUTRIAUX A; LENGAUER C ET AL.: "Requirement for p53 and p21 to Sustain G2 Arrest After DNA Damage", SCIENCE, vol. 282, 1998, pages 1497 - 1501
ADACHI N; SO S; IIIZUMI S ET AL.: "The human pre-B cell line Nalm-6 is highly proficient in gene targeting by homologous recombination", DNA CELL BIOL., vol. 25, 2006, pages 19 - 24
IIIZUMI, S; NOMURA, Y; SO, S ET AL.: "Simple one-week method to construct gene-targeting vectors: application to production of human knockout cell lines", BIOTECHNIQUES, vol. 41, 2006, pages 311 - 316
ADACHI, N; SO, S; IIIZUMI, S ET AL.: "The human pre-B cell line Nalm-6 is highly proficient in gene targeting by homologous recombination", DNA CELL BIOL., vol. 25, 2006, pages 19 - 24
ADACHI, N; NISHIJIMA, H; SHIBAHARA, K: "Gene targeting using the human Nalm-6 pre-B cell line", BIOSCIENCE TRENDS., vol. 2, 2008, pages 169 - 180
TOYODA, E; KAGAYA, S; COWELL, IG ET AL.: "NK314, a topoisomerase II inhibitor that specifically targets the alpha isoform", J. BIOL. CHEM., vol. 283, 2008, pages 23711 - 23720
IIIZUMI, S; NOMURA, Y; SO, S ET AL.: "Simple one-week method to construct gene- targeting vectors: application to production of human knockout cell lines", BIOTECHNIQUES, vol. 41, 2006, pages 311 - 316
FRIEDRICH, G.; SORIANO P, GENES DEV., vol. 5, 1991, pages 1513 - 1523
IIIZUMI ET AL., NUCLEIC ACIDS RES., vol. 36, no. 19, November 2008 (2008-11-01), pages 6333 - 6342
See also references of EP 2792744A4
Attorney, Agent or Firm:
MAYAMA, Setsuko et al. (JP)
Setsuko Mayama (JP)
Setsuko Mayama (JP)
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Claims: