GENE THERAPY COMPOSITION AND TREATMENT FOR MYH7 -LINKED

CARDIOMYOPATHY

CROSS-REFERENCE TO RELATED APPLICATION(S)

[0001] This application claims the benefit of priority of U.S. Provisional Patent Application Serial No. 62/945,518, filed on December 9, 2019, the disclosure of which is hereby incorporated by reference herein in its entirety.

FIELD OF THE INVENTION

[0002] The present invention relates to the treatment of cardiac diseases (e.g., cardiac myopathies), and, more specifically, to gene therapy methods and pharmaceutical compositions for the treatment of hypertrophic cardiomyopathy.

BACKGROUND OF THE INVENTION

[0003] Despite pharmacologic advances in the treatment of various heart conditions, such as heart failure, mortality, and morbidity remain unacceptably high. Furthermore, certain therapeutic approaches are not suitable for many patients (e.g., ones who have an advanced heart failure condition associated with other co-morbid diseases). Alternative approaches, such as gene therapy and cell therapy, have attracted increased attention due to their potential to be uniquely tailored and efficacious in addressing the root cause pathogenesis of many cardiac diseases.

OBJECTS AND SUMMARY OF THE INVENTION [0004] It is an object of certain embodiments of the present invention to provide methods of delivering therapeutic polynucleotide sequences to cardiomyocytes of a human subject. [0005] It is a further object of certain embodiments of the present invention to utilize gene therapy methods for treating MYH7-linked cardiomyopathy.

[0006] It is a further object of certain embodiments of the present invention to vectorize a polynucleotide sequence encoding for MYH7.

[0007] It is a further object of certain embodiments to split a gene into two or more vectors that are delivered to cardiac tissue of a patient for scenarios in which the gene size exceeds the packaging capacity of the vector.

[0008] The above objects and others are met by the present invention, which in certain embodiments is directed to a method of treating or preventing cardiomyopathy in a human subject. In one aspect, the method comprises delivering a gene therapy drug to cardiac tissue of the human subject. The gene therapy drug comprises a first vector comprising a first portion of a polynucleotide sequence encoding for a therapeutic protein; and a second vector comprising a second portion of the polynucleotide sequence encoding for the therapeutic protein.

[0009] In some embodiments, the first portion and the second portion of the polynucleotide sequence collectively define the entire polynucleotide sequence from its 5’ end to its 3’ end. The first portion may comprise a first continuous sequence starting from the 5’ end and ending upstream from the 3’ end, and the second portion may comprise a second continuous sequence starting downstream from the 5’ end and ending at the 3’ end. In some embodiments, the first continuous sequence comprises a first overlap portion, the second continuous sequence comprises a second overlap portion, the first overlap portion overlaps with the second overlap portion, and the first overlap portion and the second overlap portion are single-stranded and non-complementary to each other.

[0010] In some embodiments, the therapeutic protein comprises a functional MYH7 protein, and wherein the polynucleotide sequence encodes for the functional MYH7 protein. In some embodiments, the first portion of the polynucleotide sequence comprises less than about half of the polynucleotide sequence starting from the 5’ end, and the second portion of the polynucleotide sequence comprises a remainder of the polynucleotide sequence. In some embodiments, the first portion of the polynucleotide sequence comprises more than about half of the polynucleotide sequence starting from the 5’ end, and the second portion of the polynucleotide sequence comprises a remainder of the polynucleotide sequence. In some embodiments, the first portion and the second portion of the polynucleotide sequence collectively define the polynucleotide sequence, the first portion comprises a first continuous sequence starting from the 5’ end and ending upstream from the 3’ end, the second portion comprises a second continuous sequence starting downstream from the 5’ end and ending at 3’ end, and both the first continuous sequence and the second continuous sequence are single- stranded and non-complementary to each other.

[0011] In some embodiments, the first continuous sequence comprises a first overlap portion, the second continuous sequence comprises a second overlap portion, and the first overlap portion overlaps with the second overlap portion. In some embodiments, the first overlap portion and the second overlap portion are each greater than 10 bases and less than 4,800 bases. In some embodiments, the first overlap portion and the second overlap portion encode for intron 20 of the polynucleotide sequence. In some embodiments, the first continuous sequence comprises exons 1 to 27 of the polynucleotide sequence, the second continuous sequence comprises exons 19 to 40 of the polynucleotide sequence, and the first overlap portion and the second overlap portion each comprises exons 19 to 27 of the polynucleotide sequence.

[0012] In some embodiments, the first vector further comprises a cardiac muscle-specific promotor. In some embodiments, the first vector further comprises a chimeric intron. In some embodiments, each of the first vector and the second vector comprises a viral vector. In some embodiments, one or more of the first vector or the second vector comprises one or more adeno- associated viral (AAV) vectors. In some embodiments, one or more of the first vector or the second vector comprises rAAV2/9.

[0013] In another aspect, a viral vector comprises less than an entire sequence of a polynucleotide sequence encoding for a functional MYH7 protein.

[0014] In another aspect, a method of treating or preventing hypertrophic cardiomyopathy in a human subject comprises delivering a gene therapy drug to cardiac tissue of the human subject. The gene therapy drug comprises: a first rAAV2/9 vector comprising a continuous first portion of less than all of a polynucleotide sequence encoding for a functional MYH7 protein starting from the 5’ end and ending upstream from the 3’ end; and a second rAAV2/9 vector comprising a continuous second portion of less than all of the polynucleotide sequence starting downstream from the 5’ end and ending at the 3’ end.

[0015] In another aspect, a method of treating or preventing hypertrophic cardiomyopathy in a human subject comprises delivering a first rAAV2/9 vector comprising a continuous first portion of less than all of a polynucleotide sequence encoding for a functional MYH7 protein starting from the 5’ end and ending upstream from the 3’ end. In some embodiments, the method further comprises delivering a second rAAV2/9 vector comprising a continuous second portion of less than all of the polynucleotide sequence starting downstream from the 5’ end and ending at the 3’ end.

BRIEF DESCRIPTION OF THE DRAWINGS [0016] The above and other features of the present disclosure, their nature, and various advantages will become more apparent upon consideration of the following detailed description, taken in conjunction with the accompanying drawings, in which:

[0017] FIG. 1A is a schematic illustrating two vectors sharing homologous overlapping sequences in accordance with at least one embodiment; [0018] FIG. IB is a vector map illustrating a vector encoding a first portion of an MYH7 coding region in accordance with at least one embodiment;

[0019] FIG. 1C is a vector map illustrating a vector encoding a second portion of the MYH7 coding region in accordance with at least one embodiment;

[0020] FIG. ID is a schematic illustrating the overlap region of vectors encoding for portions of MYH7 in accordance with at least one embodiment;

[0021] FIG. 2A is a schematic illustrating protein splicing based on encoded intein sequences in accordance with at least one embodiment;

[0022] FIG. 2B is a vector map illustrating a vector encoding a first portion of an MYH7 coding region and an N-intein sequence in accordance with at least one embodiment [0023] FIG. 2C is a vector map illustrating a vector encoding a second portion of the MYH7 coding region and a C-intein sequence in accordance with at least one embodiment; and

[0024] FIG. 3 is a schematic illustrating splicing of two nucleic acid coding sequences through concatemerization or homologous recombination in accordance with at least one embodiment.

DEFINITIONS

[0025] As used herein, the singular forms “a,” “an,” and “the” include plural references unless the context clearly indicates otherwise. Thus, for example, reference to “a drug” includes a single drug as well as a mixture of two or more different drugs; and reference to a “viral vector” includes a single viral vector as well as a mixture of two or more different viral vectors, and the like.

[0026] Also as used herein, “about,” when used in connection with a measured quantity, refers to the normal variations in that measured quantity, as expected by one of ordinary skill in the art in making the measurement and exercising a level of care commensurate with the objective of measurement and the precision of the measuring equipment. In certain embodiments, the term “about” includes the recited number ±10%, such that “about 10” would include from 9 to 11.

[0027] Also as used herein, “polynucleotide” has its ordinary and customary meaning in the art and includes any polymeric nucleic acid such as DNA or RNA molecules, as well as chemical derivatives known to those skilled in the art. Polynucleotides include not only those encoding a therapeutic protein, but also include sequences that can be used to decrease the expression of a targeted nucleic acid sequence using techniques known in the art (e.g., antisense, interfering, or small interfering nucleic acids). Polynucleotides can also be used to initiate or increase the expression of a targeted nucleic acid sequence or the production of a targeted protein within cells of the cardiovascular system. Targeted nucleic acids and proteins include, but are not limited to, nucleic acids and proteins normally found in the targeted tissue, derivatives of such naturally occurring nucleic acids or proteins, naturally occurring nucleic acids or proteins not normally found in the targeted tissue, or synthetic nucleic acids or proteins. One or more polynucleotides can be used in combination, administered simultaneously and/or sequentially, to increase and/or decrease one or more targeted nucleic acid sequences or proteins.

[0028] Also as used herein, “exogenous” nucleic acids or genes are those that do not occur in nature in the vector utilized for nucleic acid transfer; e.g., not naturally found in the viral vector, but the term is not intended to exclude nucleic acids encoding a protein or polypeptide that occurs naturally in the patient or host.

[0029] Also as used herein, “cardiac cell” includes any cell of the heart that is involved in maintaining a structure or providing a function of the heart such as a cardiac muscle cell, a cell of the cardiac vasculature, or a cell present in a cardiac valve. Cardiac cells include cardio myocytes (having both normal and abnormal electrical properties), epithelial cells, endothelial cells, fibroblasts, cells of the conducting tissue, cardiac pace making cells, and neurons.

[0030] Also as used herein, “adeno-associated virus” or “AAV” encompasses all subtypes, serotypes and pseudotypes, as well as naturally occurring and recombinant forms. A variety of AAV serotypes and strains are known in the art and are publicly available from sources, such as the ATCC, and academic or commercial sources. Alternatively, sequences from AAV serotypes and strains which are published and/or available from a variety of databases may be synthesized using known techniques.

[0031] Also as used herein, “serotype” refers to an AAV which is identified by and distinguished from other AAVs based on capsid protein reactivity with defined antisera. There are at least twelve known serotypes of human AAV, including AAV1 through AAV12, however additional serotypes continue to be discovered, and use of newly discovered serotypes are contemplated.

[0032] Also as used herein, “pseudotyped” AAV refers to an AAV that contains capsid proteins from one serotype and a viral genome including 5' and 3' inverted terminal repeats (ITRs) of a different or heterologous serotype. A pseudotyped recombinant AAV (rAAV) would be expected to have cell surface binding properties of the capsid serotype and genetic properties consistent with the ITR serotype. A pseudotyped rAAV may comprise AAV capsid proteins, including VP1, VP2, and VP3 capsid proteins, and ITRs from any serotype AAV, including any primate AAV serotype from AAV1 through AAV12, as long as the capsid protein is of a serotype heterologous to the serotype(s) of the ITRs. In a pseudotyped rAAV, the 5' and 3' ITRs may be identical or heterologous. Pseudotyped rAAV are produced using standard techniques described in the art.

[0033] Also as used herein, “chimeric” rAAV vector encompasses an AAV vector comprising heterologous capsid proteins; that is, a rAAV vector may be chimeric with respect to its capsid proteins VP1, VP2, and VP3, such that VP1, VP2, and VP3 are not all of the same serotype AAV. A chimeric AAV as used herein encompasses AAV wherein the capsid proteins VP1, VP2, and VP3 differ in serotypes, including for example but not limited to capsid proteins from AAV1 and AAV2; are mixtures of other parvo virus capsid proteins or comprise other virus proteins or other proteins, such as for example, proteins that target delivery of the AAV to desired cells or tissues. A chimeric rAAV as used herein also encompasses an rAAV comprising chimeric 5' and 3' ITRs.

[0034] Also as used herein, a “pharmaceutically acceptable excipient or carrier” refers to any inert ingredient in a composition that is combined with an active agent in a formulation. A pharmaceutically acceptable excipient can include, but is not limited to, carbohydrates (such as glucose, sucrose, or dextrans), antioxidants (such as ascorbic acid or glutathione), chelating agents, low-molecular weight proteins, high-molecular weight polymers, gel-forming agents, or other stabilizers and additives. Other examples of a pharmaceutically acceptable carrier include wetting agents, emulsifying agents, dispersing agents, or preservatives, which are particularly useful for preventing the growth or action of microorganisms. Various preservatives are well known and include, for example, phenol and ascorbic acid. Examples of carriers, stabilizers or adjuvants can be found in Remington’s Pharmaceutical Sciences, Mack Publishing Company, Philadelphia, Pa., 17th ed. (1985).

[0035] Also as used herein, a “patient” refers to a subject, particularly a human (but could also encompass a non-human), who has presented a clinical manifestation of a particular symptom or symptoms suggesting the need for treatment, who is treated prophylactically for a condition, or who has been diagnosed with a condition to be treated.

[0036] Also as used herein, a “subject” encompasses the definition of the term “patient” and does not exclude individuals who are otherwise healthy.

[0037] Also as used herein, “treatment of’ and “treating” include the administration of a drug with the intent to lessen the severity of or prevent a condition, e.g., heart disease. [0038] Also as used herein, “prevention of’ and “preventing” include the avoidance of the onset of a condition, e.g., heart disease.

[0039] Also as used herein, a “condition” or “conditions” refers to those medical conditions, such as heart disease, that can be treated, mitigated, or prevented by administration to a subject of an effective amount of a drug.

[0040] Also as used herein, an “effective amount” refers to the amount of a drug that is sufficient to produce a beneficial or desired effect at a level that is readily detectable by a method commonly used for detection of such an effect. In some embodiments, such an effect results in a change of at least 10% from the value of a basal level where the drug is not administered. In other embodiments, the change is at least 20%, 50%, 80%, or an even higher percentage from the basal level. As will be described below, the effective amount of a drug may vary from subject to subject, depending on age, general condition of the subject, the severity of the condition being treated, the particular drug administered, and the like. An appropriate “effective” amount in any individual case may be determined by one of ordinary skill in the art by reference to the pertinent texts and literature and/or by using routine experimentation.

[0041] Also as used herein, an “active agent” refers to any material that is intended to produce a therapeutic, prophylactic, or other intended effect, whether or not approved by a government agency for that purpose.

[0042] Recitation of ranges of values herein are merely intended to serve as a shorthand method of referring individually to each separate value falling within the range, unless otherwise indicated herein, and each separate value is incorporated into the specification as if it were individually recited herein. All methods described herein can be performed in any suitable order unless otherwise indicated herein or otherwise clearly contradicted by context. The use of any and all examples, or exemplary language (e.g., “such as”) provided herein, is intended merely to illuminate certain materials and methods and does not pose a limitation on scope. No language in the specification should be construed as indicating any non-claimed element as essential to the practice of the disclosed materials and methods.

DETAILED DESCRIPTION

[0043] Hypertrophic cardiomyopathy (HMC) is the most common inherited cardiovascular disease with a prevalence of 1 in 500 adults, and is characterized by an increased wall thickness of the left ventricle. HMC is a highly complex and heterogenous disease in its clinical variations, ranging from asymptomatic status to heart failure. [0044] HMC is accepted as a disease of the sarcomere, which is responsible for generating the molecular force of cardiomyocyte contraction by converting chemical energy of ATP hydrolysis. Seventy percent of genetic HCM cases carry mutations in 1 of 8 sarcomeric protein genes, mainly MYBPC3 and MYH7 variants, and MYH7 mutations are responsible for approximately 40% of genetic HCM cases. Importantly, childhood cases with severe HCM or a combination of HCM and dilated cardiomyopathy are not uncommon. While therapeutic strategies have been proposed for MYBPC3, no work has been done concerning MYH7 despite the fact that the presence of mutations in this gene is associated with a poor prognosis.

[0045] The present invention relates to an AAV-based approach to treat MYH7-related cardiomyopathies. The MYH7 gene is located on chromosome 14 and encodes a class II myosin expressed in slow, type 1 muscle fibers as well as in the heart muscle. Both cardiac and skeletal muscle disorders can arise from mutations in MYH7, but cardiac disease is more frequent with more than 320 mutations having been identified. MYH7 is a 23 kilobase (kb) long gene, composed of 40 exons forming one transcript of 6087 bases (NCBI Gene ID: 4625), which encodes the 1935 amino acid MYH7 protein (SEQ ID NO: 1). The protein is composed of two regions: a head and a tail. The globular head region, called the motor domain, binds to actin and ATP and is located in the N-terminal portion. The long tail region (also called the ROD domain or the light meromyosin domain-LMM) is located in the C-terminal portion and is essential for the protein dimerization and interaction with other proteins including titin, myosin-binding protein C3, myomesin-1, etc. Mutations accounting for the cardiac or skeletal muscle disorders cluster in different parts of the protein. Most cardiomyopathy related mutations being located in the globular head domain potentially affecting the binding sites for actin, while mutations linked to skeletal myopathy are usually located in the distal regions of the ROD domain.

[0046] At present, for all inherited diseases and heart failure, the only curative treatment is heart transplantation. Cardiac gene therapy with AAV-based vectors holds great promise for the treatment of MYH7-linked HCM. AAV vectors are non-pathogenic, unable to replicate on their own, persist in the host nucleus in an extra-chromosomal form, and can be delivered by intra- myocardial or intracoronary or systemic injections. AAV vectors, which have a limited packaging capacity of approximately 5 kb, have been successfully used for transgenes exceeding 5 kb by splitting the corresponding polynucleotide sequence into 2 components, whereby the 5’ component and the 3’ component overlap significantly, usually for approximately 1000 bases, to allow for cDNA concatemerization after delivery via two AAV vectors. AAV vectors have previously been used to treat HCM using a Mybpc3 -targeted knock-in (KI) mouse model in vivo.

[0047] Certain embodiments of the present disclosure relate to different approaches involving a combination of two or more AAVs in connection with AAV-mediated MYH7 gene expression in cardiomyocytes (e.g., hiPSC-derived cardiomyocytes).

[0048] In one embodiment, a first vector (e.g., a 5’ cassette) comprises a cardiac muscle- specific promoter (such as TNNT2), and a first portion (e.g., approximately half) of a polynucleotide sequence encoding for MYH7. The first vector may also include a chimeric intron to enhance transcription of the first portion of the polynucleotide sequence. Each of the two vectors may be single stranded polynucleotide sequences.

[0049] In another embodiment, the first portion of the polynucleotide sequence has a subportion that overlaps with overlaps with a subportion of the second portion of the polynucleotide sequence. For example, the polynucleotide sequence of the first vector may include a continuous sequence starting at the 5’ end of the MYH7 sequence that includes up to and including intron 23 of the MYH7 polynucleotide sequence. The polynucleotide sequence of the second vector may start from the 5’ end of intron 23 and continue continuously to the 3’ end of the MYH7 polynucleotide sequence. This particular example results in a 183 base overlap of the sequences from the two cassettes, with each being single stranded and non- complementary. In other embodiments, the overlap may be based on a different intron, such as intron 20.

[0050] In another embodiment, the first portion in the first vector corresponds to exons 1 to 27 of the MYH7 polynucleotide sequence, and the second portion in the second vector corresponds to exons 19 to 40 of the MYH7 polynucleotide sequence, thus exhibiting an overlap of 1682 bases.

[0051] It is noted that these embodiments are exemplary, and other overlaps are also contemplated. For example, it is contemplated that other ranges of exons may be spanned by each portion of the MYH7 polynucleotide sequence split across the two vectors. For example, the first vector may contain exons 1 to 35, exons 1 to 34, exons 1 to 33, etc., and similarly the second vector may contain exons 15 to 40, 16 to 40, 17 to 40, etc. Each vector may contain any range of exons provided that the number of bases per portion of the polynucleotide sequence is of a size capable of being packaged into its viral particle (e.g., less than approximately 5 kb for AAV).

[0052] In some embodiments, the first overlap portion and the second overlap portion are each greater than 10 bases and less than 4,800 bases. In some embodiments, the overlap portions may be 10 bases, 4,800 bases, or any integer number therebetween (e.g., 100 bases, 200 bases, etc.). Suitable subranges within 10 to 4,800 bases are also contemplated (e.g., 100 to 4,800, 200 to 4,800, 100 to 4,500, etc.). Moreover, embodiments utilizing more than two vectors are contemplated (e.g., the MYH7 polynucleotide sequence may be split into three separate vectors).

[0053] An “intein” is a segment of a protein capable of excising itself and joining the remaining portions (referred to as “exteins”) with a peptide bond in a process termed protein splicing. Inteins are also referred to as “protein introns.” In some embodiments, the first vector comprises a polynucleotide sequence encoding for a first protein fragment and the second vector comprises a second polynucleotide sequence encoding for a second protein fragment. The first protein fragment comprises an N-terminal MYH7 fragment having an N-intein sequence at its C-terminus, and the second protein fragment comprises a C-terminal MYH7 fragment having a C-intein sequence at its N-terminus. After the polynucleotide sequences are expressed as their respective protein fragments, the N-intein and C-intein recognize each other and self-catalyze a reaction that ligates their respective flanking MYH7 fragments, resulting in a fully-formed and functional MYH7 protein.

[0054] In some embodiments, each cassette is packaged into a suitable AAV. For example, the cassettes may each be packaged into rAAV2/9, which is a particularly efficient serotype for cardiomyocyte transduction.

[0055] Although numerous embodiments herein are described with respect to MYH7 protein, it is to be understood that the expression of additional proteins (e.g., sarcomeric proteins) is contemplated. Exemplary proteins include in addition to MYH7, without limitations, one or more of PKP2, SERCA2, MYBPC3, MYL3, MYL2, ACTC1, TPM1, TNNT2, TNNI3, TTN, FHL1, ALPK3, dystrophin, FKRP, variants thereof, or combinations thereof. The protein or proteins used may also be functional variants of the proteins mentioned herein and may exhibit a significant amino acid sequence identity compared to the original protein. For instance, the amino acid identity may amount to at least about 30%, at least about 35%, at least about 40%, at least about 45%, at least about 50%, at least about 55%, at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, or at least about 99%. In this context, the term “functional variant” means that the variant of the protein is capable of, partially or completely, fulfilling the function of the naturally occurring corresponding protein. Functional variants of a protein may include, for example, proteins that differ from their naturally occurring counterparts by one or more amino acid substitutions, deletions, or additions.

[0056] The amino acid substitutions can be conservative or non-conservative. It is preferred that the substitutions are conservative substitutions, i.e., a substitution of an amino acid residue by an amino acid of similar polarity, which acts as a functional equivalent. Preferably, the amino acid residue used as a substitute is selected from the same group of amino acids as the amino acid residue to be substituted. For example, a hydrophobic residue can be substituted with another hydrophobic residue, or a polar residue can be substituted with another polar residue having the same charge. Functionally homologous amino acids, which may be used for a conservative substitution comprise, for example, non-polar amino acids such as glycine, valine, alanine, isoleucine, leucine, methionine, proline, phenylalanine, and tryptophan. Examples of uncharged polar amino acids comprise serine, threonine, glutamine, asparagine, tyrosine and cysteine. Examples of charged polar (basic) amino acids comprise histidine, arginine, and lysine. Examples of charged polar (acidic) amino acids comprise aspartic acid and glutamic acid.

[0057] Also considered as variants are proteins that differ from their naturally occurring counterparts by one or more (e.g., 2, 3, 4, 5, 10, or 15) additional amino acids. These additional amino acids may be present within the amino acid sequence of the original protein (i.e., as an insertion), or they may be added to one or both termini of the protein. Basically, insertions can take place at any position if the addition of amino acids does not impair the capability of the polypeptide to fulfill the function of the naturally occurring protein in the treated subject. Moreover, variants of proteins also comprise proteins in which, compared to the original polypeptide, one or more amino acids are lacking. Such deletions may affect any amino acid position provided that it does not impair the ability to fulfill the normal function of the protein. [0058] Finally, variants of cardiac sarcomeric proteins (e.g., MYH7) also refer to proteins that differ from the naturally occurring protein by structural modifications, such as modified amino acids. Modified amino acids are amino acids which have been modified either by natural processes, such as processing or post-translational modifications, or by chemical modification processes known in the art. Typical amino acid modifications comprise phosphorylation, glycosylation, acetylation, O-Linked N-acetylglucosamination, glutathionylation, acylation, branching, ADP ribosylation, crosslinking, disulfide bridge formation, formylation, hydroxylation, carboxyl ation, methylation, demethylation, amidation, cyclization, and/or covalent or non-covalent bonding to phosphotidylinositol, flavine derivatives, lipoteichonic acids, fatty acids, or lipids. [0059] The therapeutic polynucleotide sequence encoding the target protein may be administered to the subject to be treated in the form of a gene therapy vector, i.e., a nucleic acid construct which comprises the coding sequence, including the translation and termination codons, next to other sequences required for providing expression of the exogenous nucleic acid such as promoters, kozak sequences, polyA signals and the like.

[0060] For example, the gene therapy vector may be part of a mammalian expression system. Useful mammalian expression systems and expression constructs are commercially available. Also, several mammalian expression systems are distributed by different manufacturers and can be employed in the present invention, such as plasmid- or viral vector based systems, e.g., LENTI-Smart™ (InvivoGen), GenScript™ Expression vectors, pAdVAntage™ (Promega), ViraPower™ Lentiviral, Adenoviral Expression Systems (Invitrogen), and adeno-associated viral expression systems (Cell Biolabs).

[0061] Gene therapy vectors for expressing an exogenous therapeutic polynucleotide sequence of the invention can be, for example, a viral or non-viral expression vector, which is suitable for introducing the exogenous therapeutic polynucleotide sequence into a cell for subsequent expression of the protein encoded by said nucleic acid. The expression vector can be an episomal vector, i.e., one that is capable of self-replicating autonomously within the host cell, or an integrating vector, i.e., one which stably incorporates into the genome of the cell. The expression in the host cell can be constitutive or regulated (e.g., inducible).

[0062] In a certain embodiment, the gene therapy vector is a viral expression vector. Viral vectors for use in the present invention may comprise a viral genome in which a portion of the native sequence has been deleted in order to introduce a heterogeneous polynucleotide without destroying the infectivity of the virus. Due to the specific interaction between virus components and host cell receptors, viral vectors are highly suitable for efficient transfer of genes into target cells. Suitable viral vectors for facilitating gene transfer into a mammalian cell can be derived from different types of viruses, for example, from an AAV, an adenovirus, a retrovirus, a herpes simplex virus, a bovine papilloma virus, a lentivirus, a vaccinia virus, a polyoma virus, a sendai virus, orthomyxovirus, paramyxovirus, papovavirus, picomavirus, pox virus, alphavirus, or any other viral shuttle suitable for gene therapy, variations thereof, and combinations thereof. [0063] “Adenovirus expression vector” or “adenovirus” is meant to include those constructs containing adenovirus sequences sufficient (a) to support packaging of the therapeutic polynucleotide sequence construct, and/or (b) to ultimately express a tissue and/or cell-specific construct that has been cloned therein. In one embodiment of the invention, the expression vector comprises a genetically engineered form of adenovirus. Knowledge of the genetic organization of adenovirus, a 36 kilobase (kb), linear, double-stranded DNA virus, allows substitution of large pieces of adenoviral DNA with foreign sequences up to 7 kb. [0064] Adenovirus growth and manipulation is known to those of skill in the art, and exhibits broad host range in vitro and in vivo. This group of viruses can be obtained in high titers, e.g., 10 ⁹ to 10 ¹¹ plaque-forming units per mL, and they are highly infective. The life cycle of adenovirus does not require integration into the host cell genome. The foreign genes delivered by adenovirus vectors are episomal and, therefore, have low genotoxicity to host cells. No side effects have been reported in studies of vaccination with wild-type adenovirus, demonstrating their safety and/or therapeutic potential as in vivo gene transfer vectors.

[0065] Retroviruses (also referred to as “retroviral vector”) may be chosen as gene delivery vectors due to their ability to integrate their genes into the host genome, transferring a large amount of foreign genetic material, infecting a broad spectrum of species and cell types and for being packaged in special cell-lines.

[0066] The retroviral genome contains three genes, gag, pol, and env that code for capsid proteins, polymerase enzyme, and envelope components, respectively. A sequence found upstream from the gag gene contains a signal for packaging of the genome into virions. Two long terminal repeat (LTR) sequences are present at the 5' and 3' ends of the viral genome. These contain strong promoter and enhancer sequences and are also required for integration in the host cell genome.

[0067] In order to construct a retroviral vector, a nucleic acid encoding a gene of interest is inserted into the viral genome in the place of certain viral sequences to produce a virus that is replication-defective. In order to produce virions, a packaging cell line is constructed containing the gag, pol, and/or env genes but without the LTR and/or packaging components. When a recombinant plasmid containing a cDNA, together with the retroviral LTR and packaging sequences is introduced into this cell line (by calcium phosphate precipitation for example), the packaging sequence allows the RNA transcript of the recombinant plasmid to be packaged into viral particles, which are then secreted into the culture media. The media containing the recombinant retroviruses is then collected, optionally concentrated, and used for gene transfer. Retroviral vectors are able to infect a broad variety of cell types. However, integration and stable expression require the division of host cells.

[0068] The retrovirus can be derived from any of the subfamilies. For example, vectors from Murine Sarcoma Virus, Bovine Leukemia, Virus Rous Sarcoma Virus, Murine Leukemia Virus, Mink-Cell Focus-Inducing Virus, Reticuloendotheliosis Virus, or Avian Leukosis Virus can be used. The skilled person will be able to combine portions derived from different retroviruses, such as LTRs, tRNA binding sites, and packaging signals to provide a recombinant retrovirus. These retroviruses are then normally used for producing transduction competent retroviral vector particles. For this purpose, the vectors are introduced into suitable packaging cell lines. Retroviruses can also be constructed for site-specific integration into the DNA of the host cell by incorporating a chimeric integrase enzyme into the retroviral particle. [0069] Because herpes simplex virus (HSV) is neurotropic, it has generated considerable interest in treating nervous system disorders. Moreover, the ability of HSV to establish latent infections in non-dividing neuronal cells without integrating into the host cell chromosome or otherwise altering the host cell’s metabolism, along with the existence of a promoter that is active during latency makes HSV an attractive vector. And though much attention has focused on the neurotropic applications of HSV, this vector also can be exploited for other tissues given its wide host range.

[0070] Another factor that makes HSV an attractive vector is the size and organization of the genome. Because HSV is large, incorporation of multiple genes or expression cassettes is less problematic than in other smaller viral systems. In addition, the availability of different viral control sequences with varying performance (temporal, strength, etc.) makes it possible to control expression to a greater extent than in other systems. It also is an advantage that the virus has relatively few spliced messages, further easing genetic manipulations.

[0071] HSV also is relatively easy to manipulate and can be grown to high titers. Thus, delivery is less of a problem, both in terms of volumes needed to attain sufficient multiplicity of infection (MOI) and in a lessened need for repeat dosing. Avirulent variants of HSV have been developed and are readily available for use in gene therapy contexts.

[0072] Lentiviruses are complex retroviruses, which, in addition to the common retroviral genes gag, pol, and env, contain other genes with regulatory or structural function. The higher complexity enables the virus to modulate its life cycle, as in the course of latent infection. Some examples of lentivirus include the Human Immunodeficiency Viruses (HIV-1, HIV-2) and the Simian Immunodeficiency Virus (SIV). Lentiviral vectors have been generated by multiply attenuating the HIV virulence genes, for example, the genes env, vif, vpr, vpu, and nef are deleted making the vector biologically safe.

[0073] Lentiviral vectors are plasmid-based or virus-based, and are configured to carry the essential sequences for incorporating foreign nucleic acid, for selection and for transfer of the nucleic acid into a host cell. The gag, pol and env genes of the vectors of interest also are known in the art. Thus, the relevant genes are cloned into the selected vector and then used to transform the target cell of interest. [0074] Vaccinia vims vectors have been used extensively because of the ease of their construction, relatively high levels of expression obtained, wide host range and large capacity for carrying DNA. Vaccinia contains a linear, double-stranded DNA genome of about 186 kb that exhibits a marked “A-T” preference. Inverted terminal repeats of about 10.5 kb flank the genome. The majority of essential genes appear to map within the central region, which is most highly conserved among poxviruses. Estimated open reading frames in vaccinia vims number from 150 to 200 Although both strands are coding, extensive overlap of reading frames is not common.

[0075] At least 25 kb can be inserted into the vaccinia vims genome. Prototypical vaccinia vectors contain transgenes inserted into the viral thymidine kinase gene via homologous recombination. Vectors are selected on the basis of a tk-phenotype. Inclusion of the untranslated leader sequence of encephalomyocarditis vims results in a level of expression that is higher than that of conventional vectors, with the transgenes accumulating at 10% or more of the infected cell’s protein in 24 hours.

[0076] The empty capsids of papovavimses, such as the mouse polyoma vims, have received attention as possible vectors for gene transfer. The use of empty polyoma was first described when polyoma DNA and purified empty capsids were incubated in a cell-free system. The DNA of the new particle was protected from the action of pancreatic DNase. The reconstituted particles were used for transferring a transforming polyoma DNA fragment to rat Fill cells. The empty capsids and reconstituted particles consist of all three of the polyoma capsid antigens VP1, VP2 and VP3.

[0077] AAVs are parvoviruses belonging to the genus Dependovims. They are small, nonenveloped, single-stranded DNA vimses which require a helper vims in order to replicate. Co-infection with a helper vims (e.g., adenovims, herpes vims, or vaccinia vims) is necessary in order to form functionally complete AAV virions. In vitro, in the absence of co-infection with a helper vims, AAV establishes a latent state in which the viral genome exists in an episomal form, but infectious virions are not produced. Subsequent infection by a helper vims “rescues” the genome, allowing it to be replicated and packaged into viral capsids, thereby reconstituting the infectious virion. Recent data indicate that in vivo both wild type AAV and recombinant AAV predominantly exist as large episomal concatemers. In one embodiment, the gene therapy vector used herein is an AAV vector. The AAV vector may be purified, replication incompetent, pseudotyped rAAV particles.

[0078] AAV are not associated with any known human diseases, are generally not considered pathogenic, and do not appear to alter the physiological properties of the host cell upon integration. AAV can infect a wide range of host cells, including non-dividing cells, and can infect cells from different species. In contrast to some vectors, which are quickly cleared or inactivated by both cellular and humoral responses, AAV vectors have been shown to induce persistent transgene expression in various tissues in vivo. The persistence of recombinant AAV-mediated transgenes in non-diving cells in vivo may be attributed to the lack of native AAV viral genes and the vector’s ITR-linked ability to form episomal concatemers.

[0079] AAV is an attractive vector system for use in the cell transduction of the present invention as it has a high frequency of persistence as an episomal concatemer and it can infect non-dividing cells, including cardiomyocytes, thus making it useful for delivery of genes into mammalian cells, for example, in tissue culture and in vivo.

[0080] Typically, rAAV is made by cotransfecting a plasmid containing the gene of interest flanked by the two AAV terminal repeats and/or an expression plasmid containing the wild- type AAV coding sequences without the terminal repeats, for example pIM45. The cells are also infected and/or transfected with adenovirus and/or plasmids carrying the adenovirus genes required for AAV helper function. Stocks of rAAV made in such fashion are contaminated with adenovirus, which must be physically separated from the rAAV particles (for example, by cesium chloride density centrifugation or column chromatography). Alternatively, adenovirus vectors containing the AAV coding regions and/or cell lines containing the AAV coding regions and/or some or all of the adenovirus helper genes could be used. Cell lines carrying the rAAV DNA as an integrated provirus can also be used.

[0081] Multiple serotypes of AAV exist in nature, with at least twelve serotypes (AAV1- AAV12). Despite the high degree of homology, the different serotypes have tropisms for different tissues. Upon transfection, AAV elicits only a minor immune reaction (if any) in the host. Therefore, AAV is highly suited for gene therapy approaches.

[0082] The present disclosure may be directed in some embodiments to a drug comprising an AAV vector that is one or more of AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV10, AAV11, AAV12, ANC AAV, chimeric AAV derived thereof, variations thereof, and combinations thereof, which will be even better suitable for high efficiency transduction in the tissue of interest. In certain embodiments, the gene therapy vector is an AAV serotype 1 vector. In certain embodiments, the gene therapy vector is an AAV serotype 2 vector. In certain embodiments, the gene therapy vector is an AAV serotype 3 vector. In certain embodiments, the gene therapy vector is an AAV serotype 4 vector. In certain embodiments, the gene therapy vector is an AAV serotype 5 vector. In certain embodiments, the gene therapy vector is an AAV serotype 6 vector. In certain embodiments, the gene therapy vector is an AAV serotype 7 vector. In certain embodiments, the gene therapy vector is an AAV serotype 8 vector. In certain embodiments, the gene therapy vector is an AAV serotype 9 vector. In certain embodiments, the gene therapy vector is an AAV serotype 10 vector. In certain embodiments, the gene therapy vector is an AAV serotype 11 vector. In certain embodiments, the gene therapy vector is an AAV serotype 12 vector.

[0083] In some embodiments, the gene therapy vector may be an AAV serotype having one or more capsid proteins disclosed in U.S. Patent Nos. 7,198,951 and 7,906,111, the disclosures of which are hereby incorporated by reference herein in their entireties.

[0084] In some embodiments, the gene therapy vector is an AAV serotype 9 vector. One or more capsid proteins of the AAV serotype 9 vector may be selected from amino acid sequences of at least one of SEQ ID NO: 2, SEQ ID NO: 3, or portions thereof (e.g., amino acids 138 to 736 or amino acids 203 to 736 of either of SEQ ID NO: 2 or SEQ ID NO: 3). [0085] One or more of the capsid proteins may be encoded by, for example, the nucleic acid sequences of SEQ ID NO: 4, SEQ ID NO: 5, or portions thereof (such as nucleotides 411 to 2211 or nucleotides 609 to 2211 of SEQ ID NO: 5).

[0086] A suitable dose of AAV for humans may be in the range of about 1x10 ⁸ vg/kg to about 3x 10 ¹⁴ vg/kg, about 1x10 ⁸ vg/kg, about 1x10 ⁹ vg/kg, about 1x10 ¹⁰ vg/kg, about 1x10 ¹¹ vg/kg, about 1x10 ¹² vg/kg, about 1x10 ¹³ vg/kg, or about 1x10 ¹⁴ vg/kg. The total amount of viral particles or DRP is, is about, is at least, is at least about, is not more than, or is not more than about, 5x10 ¹⁵ vg/kg, 4x10 ¹⁵ vg/kg, 3x10 ¹⁵ vg/kg, 2x10 ¹⁵ vg/kg, 1x10 ¹⁵ vg/kg, 9x10 ¹⁴ vg/kg, 8x10 ¹⁴ vg/kg, 7x10 ¹⁴ vg/kg, 6x10 ¹⁴ vg/kg, 5x10 ¹⁴ vg/kg, 4x10 ¹⁴ vg/kg, 3x10 ¹⁴ vg/kg, 2x10 ¹⁴ vg/kg, 1x10 ¹⁴ vg/kg, 9x10 ¹³ vg/kg, 8x10 ¹³ vg/kg, 7x10 ¹³ vg/kg, 6x10 ¹³ vg/kg,

5x10 ¹³ vg/kg, 4x10 ¹³ vg/kg, 3x10 ¹³ vg/kg, 2x10 ¹³ vg/kg, 1x10 ¹³ vg/kg, 9x10 ¹² vg/kg,

8x10 ¹² vg/kg, 7x10 ¹² vg/kg, 6x10 ¹² vg/kg, 5x10 ¹² vg/kg, 4x10 ¹² vg/kg, 3x10 ¹² vg/kg,

2x10 ¹² vg/kg, 1x10 ¹² vg/kg, 9x10 ¹¹ vg/kg, 8x10 ¹¹ vg/kg, 7x10 ¹¹ vg/kg, 6x10 ¹¹ vg/kg,

5x10 ¹¹ vg/kg, 4x10 ¹¹ vg/kg, 3x10 ¹¹ vg/kg, 2x10 ¹¹ vg/kg, 1x10 ¹¹ vg/kg, 9x10 ¹⁰ vg/kg,

8x10 ¹⁰ vg/kg, 7x10 ¹⁰ vg/kg, 6x10 ¹⁰ vg/kg, 5x10 ¹⁰ vg/kg, 4x10 ¹⁰ vg/kg, 3x10 ¹⁰ vg/kg,

2x10 ¹⁰ vg/kg, 1x10 ¹⁰ vg/kg, 9x10 ⁹ vg/kg, 8x10 ⁹ vg/kg, 7x10 ⁹ vg/kg, 6x10 ⁹ vg/kg, 5x10 ⁹ vg/kg, 4x10 ⁹ vg/kg, 3x10 ⁹ vg/kg, 2x10 ⁹ vg/kg, 1x10 ⁹ vg/kg, 9x10 ⁸ vg/kg, 8x10 ⁸ vg/kg, 7x10 ⁸ vg/kg, 6x10 ⁸ vg/kg, 5x10 ⁸ vg/kg, 4x10 ⁸ vg/kg, 3x10 ⁸ vg/kg, 2x10 ⁸ vg/kg, or 1x10 ⁸ vg/kg, or falls within a range defined by any two of these values. The above listed dosages being in vg/kg heart tissue units.

[0087] Apart from viral vectors, non-viral expression constructs may also be used for introducing a gene encoding a target protein or a functioning variant or fragment thereof into a cell of a patient. Non-viral expression vectors which permit the in vivo expression of protein in the target cell include, for example, a plasmid, a modified RNA, a cDNA, antisense oligomers, DNA-lipid complexes, nanoparticles, exosomes, any other non-viral shuttle suitable for gene therapy, variations thereof, and a combination thereof.

[0088] Apart from viral vectors and non-viral expression vectors, nuclease systems may also be used, in conjunction with a vector and/or an electroporation system, to enter into a cell of a patient and introduce therein a gene encoding a target protein or a functioning variant or fragment thereof. Exemplary nuclease systems may include, without limitations, a clustered regularly interspaced short palindromic repeats (CRISPR), a DNA cutting enzyme (e.g., Cas9), meganucleases, TALENs, zinc finger nucleases, any other nuclease system suitable for gene therapy, variations thereof, and a combination thereof. For instance, in one embodiment, one viral vector (e.g., AAV) may be used for a nuclease (e.g., CRISPR) and another viral vector (e.g., AAV) may be used for a DNA cutting enzyme (e.g., Cas9) to introduce both (the nuclease and the DNA cutting enzyme) into a target cell.

[0089] Other vector delivery systems which can be employed to deliver a therapeutic polynucleotide sequence encoding a therapeutic gene into cells are receptor-mediated delivery vehicles. These take advantage of the selective uptake of macromolecules by receptor-mediated endocytosis in almost all eukaryotic cells. Because of the cell type-specific distribution of various receptors, the delivery can be highly specific. Receptor-mediated gene targeting vehicles may include two components: a cell receptor-specific ligand and a DNA-binding agent.

[0090] Suitable methods for the transfer of non-viral vectors into target cells are, for example, the lipofection method, the calcium-phosphate co-precipitation method, the DEAE- dextran method and direct DNA introduction methods using micro-glass tubes, ultrasound, electroporation, and the like. Prior to the introduction of the vector, the cardiac muscle cells may be treated with a permeabilization agent, such as phosphatidylcholine, streptolysins, sodium caprate, decanoylcarnitine, tartaric acid, lysolecithin, Triton X-100, and the like. Exosomes may also be used to transfer naked DNA or AAV-encapsidated DNA.

[0091] A gene therapy vector of the invention may comprise a promoter that is functionally linked to the nucleic acid sequence encoding to the target protein. The promoter sequence must be compact and ensure a strong expression. Preferably, the promoter provides for an expression of the target protein in the myocardium of the patient that has been treated with the gene therapy vector. In some embodiment, the gene therapy vector comprises a cardiac-specific promoter that is operably linked to the nucleic acid sequence encoding the target protein. As used herein, a “cardiac-specific promoter” refers to a promoter whose activity in cardiac cells is at least 2- fold higher than in any other non-cardiac cell type. Preferably, a cardiac-specific promoter suitable for being used in the vector of the invention has an activity in cardiac cells which is at least 5-fold, at least 10-fold, at least 15-fold, at least 20-fold, at least 25-fold, or at least 50-fold higher compared to its activity in a non-cardiac cell type.

[0092] The cardiac-specific promoter may be a selected human promoter, or a promoter comprising a functionally equivalent sequence having at least about 80%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, or at least about 99% sequence identity to the selected human promoter. An exemplary non-limiting promoter that may be used is a cardiac troponin T promoter (TNNT2). Other non-limiting examples of promoters include the alpha myosin heavy chain promoter, the myosin light chain 2v promoter, the alpha myosin heavy chain promoter, the alpha-cardiac actin promoter, the alpha- tropomyosin promoter, the cardiac troponin C promoter, the cardiac troponin I promoter, the cardiac myosin-binding protein C promoter, and the sarco/endoplasmic reticulum Ca ²⁺ -ATPase (SERCA) promoter (e.g., isoform 2 of this promoter (SERCA2)).

[0093] The vectors useful in the present invention may have varying transduction efficiencies. As a result, the viral or non-viral vector transduces more than, equal to, or at least about 10%, about 20%, about 30%, about 40%, about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90%, about 95%, about 99%, or 100% of the cells of the targeted vascular territory. More than one vector (viral or non-viral, or combinations thereof) can be used simultaneously or in sequence. This can be used to transfer more than one polynucleotide, and/or target more than one type of cell. Where multiple vectors or multiple agents are used, more than one transduction/transfection efficiency can result. [0094] Pharmaceutical compositions that contain gene therapy vectors may be prepared either as liquid solutions or suspensions. The pharmaceutical composition of the invention can include commonly used pharmaceutically acceptable excipients, such as diluents and carriers. In particular, the composition comprises a pharmaceutically acceptable carrier, e.g., water, saline, Ringer’s solution, or dextrose solution. In addition to the carrier, the pharmaceutical composition may also contain emulsifying agents, pH buffering agents, stabilizers, dyes and the like.

[0095] In certain embodiments, a pharmaceutical composition will comprise a therapeutically effective gene dose, which is a dose that is capable of preventing or treating cardiomyopathy in a subject, without being toxic to the subject. Prevention or treatment of cardiomyopathy may be assessed as a change in a phenotypic characteristic associated with cardiomyopathy with such change being effective to prevent or treat cardiomyopathy. Thus, a therapeutically effective gene dose is typically one that, when administered in a physiologically tolerable composition, is sufficient to improve or prevent the pathogenic heart phenotype in the treated subject.

[0096] In certain embodiments, gene therapy vectors may be transduced into a subject through several different methods, including intravenous delivery, intraarterial delivery, or intraperitoneal delivery. In some embodiments, a gene therapy vector may be administered directly to heart tissue, for example, by intracoronary administration. In some embodiments, tissue transduction of the myocardium may be achieved by catheter-mediated intramyocardial delivery, which may be used to transfer vector-free cDNA coupled to or uncoupled to transduction-enhancing carriers into myocardium.

[0097] In certain embodiments, the drug will comprise a therapeutically effective gene dose. A therapeutically effective gene dose is one that is capable of preventing or treating a particular heart condition in a patient, without being toxic to the patient.

[0098] Heart conditions that may be treated by the methods disclosed herein may include, without limitations, one or more of a genetically determined heart disease (e.g., genetically determined cardiomyopathy), arrhythmic heart disease, heart failure, ischemia, arrhythmia, myocardial infarction, congestive heart failure, transplant rejection, abnormal heart contractility, non-ischemic cardiomyopathy, mitral valve regurgitation, aortic stenosis or regurgitation, abnormal Ca ²⁺ metabolism, congenital heart disease, primary or secondary cardiac tumors, and combinations thereof.

ILLUSTRATIVE PROPHETIC EXAMPLES [0099] The following example is set forth to assist in understanding the disclosure and should not, of course, be construed as specifically limiting the embodiments described and claimed herein. Such variations of the embodiments, including the substitution of all equivalents now known or later developed, which would be within the purview of those skilled in the art, and changes in formulation or minor changes in experimental design, are to be considered to fall within the scope of the embodiments incorporated herein.

[0100] In general, in vitro transduction efficiency may be assessed by qPCR, and quantification of concatamerization splicing events may be analyzed using specific primers and probes overlapping exonic junctions. Example 1: Homologous overlapping sequences

[0101] In this example, the transgene is split into two AAV vectors sharing homologous overlapping sequences, such that the reconstitution of MYH7 relies on homologous recombination. The overlap length can be adjusted, as discussed throughout this disclosure.

In these example constructs, as illustrated in FIGS. 1 A-1D, the size between the two ITR sequences is 4712 bases for the first AAV vector (FIG. IB) and 4351 bases for the second AAV vector (FIG. 1C). The length of the overlap is 1055 bases (FIG. ID).

[0102] SEQ ID NO: 6 corresponds to the first AAV vector of FIG. IB having a Zeomycin resistance (ZeoR) gene removed, and SEQ ID NO: 7 corresponds to the second AAV vector of FIG. 1C.

Example 2: Inteins

[0103] In this example, protein splicing occurs based on encoded intein sequences. The splicing event is an autocatalytic process where the intein excises itself from the primary/precursor protein and then catalyzes the joining of the broken ends forming two protein products: the mature protein and the intein itself.

[0104] In these example constructs, as illustrated in FIGS. 2A-2C, the first AAV vector encodes the first N-terminal 946 amino acids (FIG. 2B, not to scale) and the second AAV vector encodes the C-terminal amino acids 947-1935 (FIG. 2C, not to scale), though it is contemplated that other combinations of sequence ranges are possible. The size between the two ITR sequences is 4923 bases for the first AAV vector (FIG. 2B, SEQ ID NO: 8) and 4819 bases for the second AAV vector (FIG. 2C, SEQ ID NO: 9). MYH7 expression is under the control of the TNNT2 promoter. Flag, ZeoR, and Blasticidin are used to select the transduced cells.

Example 3: Hybrid homologous recombination and RNA splicing

[0105] This example combines two approaches: homologous recombination and RNA splicing. A highly recombinogenic exogenous sequence is used to trigger the homologous recombination. This sequence is spliced out after transcription because it will be recognized as an intron in the pre-mRNA. This sequence was placed between exons 20 and 21, though other possible insertion locations may exist and are contemplated. This sequence is derived from the alkaline phosphatase gene (SEAP).

[0106] In these example constructs, as illustrated in FIG. 3, the first AAV vector (SEQ ID NO: 10) contains the TNNT2 promoter driving the transcription of the first 20 exons of MYH7. An optimized sequenced is generally used to improve protein translation in the other examples, but in this example sequences of exons 20 and 21 are not optimized. Exon 20 is followed by the first 40 bases of endogenous intron 20, followed by the first 272 bases of the SEAP gene. The selection gene, chimeric intron, and flag are also present to improve transcription/translation efficacy and imaging.

[0107] The second AAV vector (SEQ ID NO: 11) starts with the same 272 bases from the SEAP (for HR), followed by the last 40 bases of endogenous intron 20 and the whole non- optimized exon 21 of MYH7, followed by the sequence coding optimized exons 22-40 of MYH7.

[0108] In the foregoing description, numerous specific details are set forth, such as specific materials, dimensions, processes parameters, etc., to provide a thorough understanding of the present invention. The particular features, structures, materials, or characteristics may be combined in any suitable manner in one or more embodiments. The words “example” or “exemplary” are used herein to mean serving as an example, instance, or illustration. Any aspect or design described herein as “example” or “exemplary” is not necessarily to be construed as preferred or advantageous over other aspects or designs. Rather, use of the words “example” or “exemplary” is simply intended to present concepts in a concrete fashion. As used in this application, the term “or” is intended to mean an inclusive “or” rather than an exclusive “or”. That is, unless specified otherwise, or clear from context, “X includes A or B” is intended to mean any of the natural inclusive permutations. That is, if X includes A; X includes B; or X includes both A and B, then “X includes A or B” is satisfied under any of the foregoing instances. Reference throughout this specification to “an embodiment”, “certain embodiments”, or “one embodiment” means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one embodiment. Thus, the appearances of the phrase “an embodiment”, “certain embodiments”, or “one embodiment” in various places throughout this specification are not necessarily all referring to the same embodiment.

[0109] The present invention has been described with reference to specific exemplary embodiments thereof. The specification and drawings are, accordingly, to be regarded in an illustrative rather than a restrictive sense. Various modifications of the invention in addition to those shown and described herein will become apparent to those skilled in the art and are intended to fall within the scope of the appended claims. [0110] SEQ ID NO: 1 below is an amino acid sequence encoding for MYH7:

MGD SEMAVF GAAAPYLRKSEKERLEAQTRPFDLKKD VF VPDDKQEF VKAKIV SREG

GK VT AETEY GKT VT VKEDQ VMQQNPPKFDKIEDMAMLTFLHEP AVL YNLKDRY GS

WMIYT YSGLF C VTVNPYKWLP VYTPEVVAAYRGKKRSEAPPtllF SISDNAY QYMLT

DRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKDQSPGKGTLEDQIIQAN PA

LEAF GNAKTVRNDN S SRF GKFIRIHFGATGKL AS ADIETYLLEKSRVIF QLKAERDYHI

FYQILSNKKPELLDMLLITNNPYDYAFISQGETTVASIDDAEELMATDNAFDVLGFT S

EEKN SMYKLTGAIMHF GNMKFKLKQREEQ AEPDGTEEADKS AYLMGLN S ADLLKG

LCHPRVK V GNE Y VTKGQN VQQ VI Y AT GAL AK A VYERMFNWM VTRIN ATLETKQPR

QYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTF ID

F GMDLQ ACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLFDNHLGKS ANF QKPRNI

KGKPE AHF SLIH Y AGIVD YNIIGWLQKNKDPLNET V V GL Y QK S SLKLL S TLF ANY AG

AD APIEKGKGKAKKGS SF QTV S ALHRENLNKLMTNLRSTHPHF VRCIIPNETKSPGV

MDNPLVMHQLRCNGVLEGIRICRKGFPNRIL Y GDFRQRYRILNP AAIPEGQFID SRKG

AEKLL S SLDIDHN Q YKF GHTK VFFK AGLLGLLEEMRDERL SRIITRIQ AQ SRGVL ARM

EYKKLLERRDSLLVIQWNIRAFMGVKNWPWMKLYFKIKPLLKSAEREKEMASMKE

EFTRLKEALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLADAEERCDQLIK

NKIQLEAKVKEMNERLEDEEEMNAELTAKKRKLEDECSELKRDIDDLELTLAKVEK

EKHATENKVKNLTEEMAGLDEIIAKLTKEKKALQEAHQQALDDLQAEEDKVNTLTK

AKVKLEQQVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKQQLD

ERLKKKDFELNALNARIEDEQALGSQLQKKLKELQARIEELEEELEAERTARAKVEK

LRSDL SRELEEI SERLEE AGGAT S VQIEMNfCKRE AEF QKMRRDLEE ATLQHE AT AAA

LRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEK

MCRTLEDQMNEHRSKAEETQRSVNDLTSQRAKLQTENGELSRQLDEKEALISQLTR

GKLT YTQQLEDLKRQLEEEVKAKNAL AHALQ S ARHDCDLLREQ YEEETEAK AELQR

VLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQEAEEAVEAVNAKCSSLE

KTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESSQ

KEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGSSGKTIHELEK VR

KQLEAEKMELQSALEEAEASLEHEEGKILRAQLEFNQIKAEIERKLAEKDEEMEQAK

RNHLRVVDSLQTSLDAETRSRNEALRVKKKMEGDLNEMEIQLSHANRMAAEAQKQ

VKSLQSLLKDTQIQLDDAVRANDDLKENIAIVERRNNLLQAELEELRAVVEQTERSR

KL AEQELIET SERV QLLHS QNT SLIN QKKKMD ADL S QLQTE VEE A V QECRN AEEK AK

KAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQ LQKLEARVRELENELEAEQKRNAESVKGMRKSERRIKELTYQTEEDRKNLLRLQDL VDKLQLK VK A YKRQ AEE AEEQ ANTNL SKFRK V QHELDE AEERADIAE S Q VNKLR A KSRDIGTKGLNEE

[0111] SEQ ID NO: 2 below is an amino acid sequence encoding for an AAV serotype 9 capsid protein.

MAADGYLPDWLEDNLSEGIREWWDLKPGAPKPKANQQKQDDGRGLVLPGYKYLG PFNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLRYNHADAEFQERLQEDTS F GGNLGRA VF Q AKKRVLEPLGL VEEGAKT APGKKRP VEQ SPQEPD S S S GIGK S GQQP AKKRLNF GQTGD SES VPDPQPLGEPPEAP SGLGPNTM ASGGGAPMADNNEGADGVG NS S GNWHCD ST WLGDRVITT S TRTW ALPT YNNHL YKQI SN GT S GGS TNDNT YF GY S TPW GYFDFNRFHCHF SPRD W QRLINNNW GFRPKRLNFKLFNIQ VKE VTTNEGTKTI A NNLTSTVQVFTDSEYQLPYVLGSAHQGCLPPFPADVFMVPQYGYLTLNNGSQALGR S SF Y CLEYFP SQMLRTGNNF QF S YTFED VPFHS S YAHSQ SLDRLMNPLIDQ YL YYL VR TQTTGTGGTQTLAFSQAGPSSMANQARNWVPGPCYRQQRVSTTTNQNNNSNFAWT GAAKFKLN GRD SLMNPGVAMASHKDDEDRFFPS SGVLIFGKQGAGNDGVD YSQVLI TDEEEIKATNPVATEEYGAVAINNQAANTQAQTGLVHNQGVIPGMVWQNRDVYLQ GPIWAKIPHTDGNFHPSPLMGGFGLKHPPPQILIKNTPVPADPPLTFNQAKLNSFITQY S T GQ V S VEIEWEL QKEN SKRWNPEIQ YT SN Y YK S TN VDF A VNTEG V Y SEPRPIGTR Y LTRNL

[0112] SEQ ID NO: 3 below is a further amino acid sequence encoding for an AAV serotype 9 capsid protein:

[0113] MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQHQDNARGLVLP GYKYLGPGNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLKYNHADAEFQE RLKEDTSF GGNLGRAVF Q AKKRLLEPLGLVEEAAKT APGKKRP VEQ SPQEPD S S AGI GKSGAQPAKKRLNFGQTGDTESVPDPQPIGEPPAAPSGVGSLTMASGGGAPVADNN EGADGV GS S S GNWHCD S Q WLGDR VITT S TRT W ALPT YNNHL YKQI SNST S GGS SND N A YF GY S TPW GYFDFNRFHCHF SPRD W QRLINNNW GFRPKRLNFKLFNIQ VKE VTD NN GVKTI ANNLT S TV Q VF TD SD Y QLP YVLGS AHEGCLPPFP AD VFMIPQ Y GYLTLND GSQ AVGRS SF Y CLEYFP SQMLRT GNNF QF S YEFENVPFHS S YAHSQ SLDRLMNPLID QYLYYLSKTINGSGQNQQTLKFSVAGPSNMAVQGRNYIPGPSYRQQRVSTTVTQNN NSEFAWPGASSWALNGRNSLMNPGPAMASHKEGEDRFFPLSGSLIFGKQGTGRDNV DADKVMITNEEEIKTTNPVATESYGQVATNHQSAQAQAQTGWVQNQGILPGMVWQ DRD VYLQGPIWAKIPHTDGNFHP SPLMGGF GMKHPPPQILIKNTP VP ADPPT AFNKD KLN SFITQYST GQ V S VEIEWELQKEN SKRWNPEIQ YT SN YYK SNNVEF A VNTEGV Y S EPRPIGTRYLTRNL

[0114] SEQ ID NO: 4 below is a nucleic acid sequence encoding for an AAV serotype capsid protein:

ATGGCTGCCGATGGTTATCTTCCAGATTGGCTCGAGGACAACCTCTCTGAGGGCA

TTCGCGAGTGGTGGGACCTGAAACCTGGAGCCCCGAAACCCAAAGCCAACCAGC

AAAAGCAGGACGACGGCCGGGGTCTGGTGCTTCCTGGCTACAAGTACCTCGGAC

CCTTCAACGGACTCGACAAGGGGGAGCCCGTCAACGCGGCGGACGCAGCGGCCC

TCGAGCACGACAAGGCCTACGACCAGCAGCTCAAAGCGGGTGACAATCCGTACC

TGCGGTATAACCACGCCGACGCCGAGTTTCAGGAGCGTCTGCAAGAAGATACGT

CTTTTGGGGGCAACCTCGGGCGAGCAGTCTTCCAGGCCAAGAAGCGGGTTCTCG

AACCTCTCGGTCTGGTTGAGGAAGGCGCTAAGACGGCTCCTGGAAAGAAGAGAC

CGGTAGAGCAGTCACCCCAAGAACCAGACTCATCCTCGGGCATCGGCAAATCAG

GCCAGCAGCCCGCTAAAAAGAGACTCAATTTTGGTCAGACTGGCGACTCAGAGT

CAGTCCCCGACCCACAACCTCTCGGAGAACCTCCAGAAGCCCCCTCAGGTCTGG

GACCTAATACAATGGCTTCAGGCGGTGGCGCTCCAATGGCAGACAATAACGAAG

GCGCCGACGGAGTGGGTAATTCCTCGGGAAATTGGCATTGCGATTCCACATGGCT

GGGGGACAGAGTCATCACCACCAGCACCCGAACCTGGGCATTGCCCACCTACAA

CAACCACCTCTACAAGCAAATCTCCAATGGAACATCGGGAGGAAGCACCAACGA

CAACACCTACTTTGGCTACAGCACCCCCTGGGGGTATTTTGACTTCAACAGATTC

CACTGCCACTTCTCACCACGTGACTGGCAGCGACTCATCAACAACAACTGGGGAT

TCCGGCCAAAGAGACTCAACTTCAAGCTGTTCAACATCCAGGTCAAGGAGGTTA

CGACGAACGAAGGCACCAAGACCATCGCCAATAACCTTACCAGCACCGTCCAGG

TCTTTACGGACTCGGAGTACCAGCTACCGTACGTCCTAGGCTCTGCCCACCAAGG

ATGCCTGCCACCGTTTCCTGCAGACGTCTTCATGGTTCCTCAGTACGGCTACCTG

ACGCTCAACAATGGAAGTCAAGCGTTAGGACGTTCTTCTTTCTACTGTCTGGAAT

ACTTCCCTTCTCAGATGCTGAGAACCGGCAACAACTTTCAGTTCAGCTACACTTT

CGAGGACGTGCCTTTCCACAGCAGCTACGCACACAGCCAGAGTCTAGATCGACT

GATGAACCCCCTCATCGACCAGTACCTATACTACCTGGTCAGAACACAGACAACT

GGAACTGGGGGAACTC AAACTTT GGC ATT C AGCC AAGC AGGCCCT AGCTC AAT G

GCCAATCAGGCTAGAAACTGGGTACCCGGGCCTTGCTACCGTCAGCAGCGCGTC

TCCACAACCACCAACCAAAATAACAACAGCAACTTTGCGTGGACGGGAGCTGCT

AAATTCAAGCTGAACGGGAGAGACTCGCTAATGAATCCTGGCGTGGCTATGGCA

TCGCACAAAGACGACGAGGACCGCTTCTTTCCATCAAGTGGCGTTCTCATATTTG

GC AAGC AAGGAGCCGGGAACGATGGAGTCGACTAC AGCC AGGTGCTGATTACAG

ATGAGGAAGAAATTAAAGCCACCAACCCTGTAGCCACAGAGGAATACGGAGCA GTGGCCATCAACAACCAGGCCGCTAACACGCAGGCGCAAACTGGACTTGTGCAT

AACCAGGGAGTTATTCCTGGTATGGTCTGGCAGAACCGGGACGTGTACCTGCAG

GGCCCTATTTGGGCTAAAATACCTCACACAGATGGCAACTTTCACCCGTCTCCTC

TGATGGGTGGATTTGGACTGAAACACCCACCTCCACAGATTCTAATTAAAAATAC

ACCAGTGCCGGCAGATCCTCCTCTTACCTTCAATCAAGCCAAGCTGAACTCTTTC

ATCACGCAGTACAGCACGGGACAAGTCAGCGTGGAAATCGAGTGGGAGCTGCAG

AAAGAAAACAGCAAGCGCTGGAATCCAGAGATCCAGTATACTTCAAACTACTAC

AAATCTACAAATGTGGACTTTGCTGTCAATACCGAAGGTGTTTACTCTGAGCCTC

GCCCCATTGGTACTCGTTACCTCACCCGTAATTTG

[0115] SEQ ID NO: 5 below is a further nucleic acid sequence encoding for an AAV serotype capsid protein:

ATGGCTGCCGATGGTTATCTTCCAGATTGGCTCGAGGACAACCTTAGTGAAGGAA

TTCGCGAGTGGTGGGCTTTGAAACCTGGAGCCCCTCAACCCAAGGCAAATCAAC

AACATCAAGACAACGCTCGAGGTCTTGTGCTTCCGGGTTACAAATACCTTGGACC

CGGCAACGGACTCGACAAGGGGGAGCCGGTCAACGCAGCAGACGCGGCGGCCC

TCGAGCACGACAAGGCCTACGACCAGCAGCTCAAGGCCGGAGACAACCCGTACC

TCAAGTACAACCACGCCGACGCCGAGTTCCAGGAGCGGCTCAAAGAAGATACGT

CTTTTGGGGGCAACCTCGGGCGAGCAGTCTTCCAGGCCAAAAAGAGGCTTCTTG

AACCTCTTGGTCTGGTTGAGGAAGCGGCTAAGACGGCTCCTGGAAAGAAGAGGC

CTGTAGAGCAGTCTCCTCAGGAACCGGACTCCTCCGCGGGTATTGGCAAATCGG

GTGCACAGCCCGCTAAAAAGAGACTCAATTTCGGTCAGACTGGCGACACAGAGT

CAGTCCCAGACCCTCAACCAATCGGAGAACCTCCCGCAGCCCCCTCAGGTGTGG

GATCTCTTACAATGGCTTCAGGTGGTGGCGCACCAGTGGCAGACAATAACGAAG

GTGCCGATGGAGTGGGTAGTTCCTCGGGAAATTGGCATTGCGATTCCCAATGGCT

GGGGGACAGAGTCATCACCACCAGCACCCGAACCTGGGCCCTGCCCACCTACAA

CAATCACCTCTACAAGCAAATCTCCAACAGCACATCTGGAGGATCTTCAAATGAC

AACGCCTACTTCGGCTACAGCACCCCCTGGGGGTATTTTGACTTCAACAGATTCC

ACTGCCACTTCTCACCACGTGACTGGCAGCGACTCATCAACAACAACTGGGGATT

CCGGCCTAAGCGACTCAACTTCAAGCTCTTCAACATTCAGGTCAAAGAGGTTACG

GACAACAATGGAGTCAAGACCATCGCCAATAACCTTACCAGCACGGTCCAGGTC

TTCACGGACTCAGACTATCAGCTCCCGTACGTGCTCGGGTCGGCTCACGAGGGCT

GCCTCCCGCCGTTCCCAGCGGACGTTTTCATGATTCCTCAGTACGGGTATCTGAC

GCTTAATGATGGAAGCCAGGCCGTGGGTCGTTCGTCCTTTTACTGCCTGGAATAT

TTCCCGTCGCAAATGCTAAGAACGGGTAACAACTTCCAGTTCAGCTACGAGTTTG

AGAACGTACCTTTCCATAGCAGCTACGCTCACAGCCAAAGCCTGGACCGACTAA

TGAATCCACTCATCGACCAATACTTGTACTATCTCTCAAAGACTATTAACGGTTC

T GGAC AGA AT C A AC A A AC GCT A A A ATT C AGT GT GGCC GGACC C AGC A AC AT GGC

TGTCCAGGGAAGAAACTACATACCTGGACCCAGCTACCGACAACAACGTGTCTC

AACCACTGTGACTCAAAACAACAACAGCGAATTTGCTTGGCCTGGAGCTTCTTCT

TGGGCTCTCAATGGACGTAATAGCTTGATGAATCCTGGACCTGCTATGGCCAGCC

ACAAAGAAGGAGAGGACCGTTTCTTTCCTTTGTCTGGATCTTTAATTTTTGGCAA

ACAAGGAACTGGAAGAGACAACGTGGATGCGGACAAAGTCATGATAACCAACG

AAGAAGAAATTAAAACTACTAACCCGGTAGCAACGGAGTCCTATGGACAAGTGG CCACAAACCACCAGAGTGCCCAAGCACAGGCGCAGACCGGCTGGGTTCAAAACC

AAGGAAT ACTTCCGGGT AT GGTTTGGC AGGAC AGAGATGT GT ACCTGC AAGGAC

CCATTTGGGCCAAAATTCCTCACACGGACGGCAACTTTCACCCTTCTCCGCTGAT

GGGAGGGTTTGGAATGAAGCACCCGCCTCCTCAGATCCTCATCAAAAACACACC

TGTACCTGCGGATCCTCCAACGGCCTTCAACAAGGACAAGCTGAACTCTTTCATC

ACCCAGTATTCTACTGGCCAAGTCAGCGTGGAGATCGAGTGGGAGCTGCAGAAG

GAAAACAGCAAGCGCTGGAACCCGGAGATCCAGTACACTTCCAACTATTACAAG

TCTAATAATGTTGAATTTGCTGTTAATACTGAAGGTGTATATAGTGAACCCCGCC

CCATTGGCACCAGATACCTGACTCGTAATCTGTAA

[0116] SEQ ID NO: 6 below is an AAV vector encoding a first portion of MYH7 that includes a homologous overlapping sequence:

GGC ACTGGGC AGGT A AGT ATC A AGGTT AC A AG AC AGGTTT A AGGAGAC C A AT AG

AAACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTG

GTCTTACTGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAAT

TACAGCTCTTAAGGCTAGAGTACTTAATACGACTCACTATAGGCTAGCGGTACCG

GTCGCCACCATGGACTACAAAGACCATGACGGTGATTATAAAGATCATGACATC

GATT AC AAGGAT GACGAT GAC A AGCTTGGT ACCGAGCTCGGATCC ATGGGAGAT

TCTGAAATGGCCGTCTTCGGCGCTGCCGCACCATATCTCCGCAAAAGTGAAAAG

GAGCGCCTGGAGGCCCAAACCAGACCCTTCGATCTGAAAAAGGATGTGTTTGTC

CCGGATGACAAGCAAGAATTTGTAAAAGCAAAGATCGTCTCTCGGGAGGGCGGT

AAAGTGACTGCTGAAACAGAATACGGCAAAACCGTCACTGTCAAAGAAGATCAG

GTAATGCAACAAAATCCTCCGAAATTTGATAAAATCGAAGACATGGCCATGTTG

ACGTTTTTGCATGAGCCAGCTGTTTTGTATAATCTGAAGGATCGGTACGGATCCT

GGATGATTTACACATACTCAGGCTTGTTCTGCGTGACCGTTAATCCCTATAAGTG

GCTGCCTGTCTACACGCCGGAGGTTGTTGCAGCATATAGGGGGAAAAAGAGGTC

CGAAGCCCCTCCCCACATCTTCTCCATTTCTGACAACGCCTACCAGTATATGCTG

ACTGACAGGGAGAACCAAAGCATTCTTATTACCGGAGAGTCTGGGGCGGGCAAG

ACGGTAAATACAAAACGCGTGATACAGTACTTTGCAGTTATTGCTGCGATTGGCG

AC AGGAGT A AAAAGGAT C AGAGCCCCGGC A AGGGT ACTCTCGAAGAT C AAATT A

TCCAGGCTAATCCCGCCCTCGAAGCGTTCGGGAATGCCAAAACGGTCCGCAATG

ATAACAGTAGCAGATTTGGGAAGTTTATCCGCATCCATTTCGGAGCGACTGGCAA

ACTCGCCTCAGCAGACATCGAGACATACCTGTTGGAAAAGTCACGAGTGATATTT

CAGCTCAAAGCCGAGCGGGACTATCATATCTTTTACCAGATACTTTCTAACAAAA

AACCAGAGCTTCTTGACATGCTTCTGATCACTAACAATCCGTATGACTATGCATT

CATCAGTCAGGGAGAGACCACGGTTGCGTCAATAGACGATGCTGAGGAGCTGAT

GGCCACAGACAATGCCTTCGATGTGCTCGGATTTACTAGCGAGGAGAAGAACAG

CATGTACAAGCTTACCGGTGCTATCATGCACTTCGGCAATATGAAATTTAAACTC

AAGCAGCGGGAAGAACAGGCGGAGCCGGACGGAACTGAAGAGGCTGATAAAAG

TGCCTACCTCATGGGGCTTAACTCTGCCGACTTGCTTAAAGGATTGTGTCATCCA

CGGGT A A AGGT AGGT A AT GA AT AT GT A ACT A AGGGT C A A A AT GT GCA AC A AGT C

ATATACGCGACTGGGGCTCTGGCAAAAGCCGTTTACGAGAGAATGTTTAACTGG

ATGGTCACACGAATTAATGCCACTCTGGAGACAAAACAACCCCGGCAGTACTTT

ATAGGTGTGCTGGATATCGCAGGTTTCGAAATTTTCGATTTCAACTCTTTCGAGC AACTTTGTATCAATTTCACCAACGAGAAGCTCCAACAATTTTTTAACCATCATAT

GTTCGTTCTCGAGCAAGAGGAATACAAAAAAGAGGGCATCGAATGGACTTTCAT

CGATTTCGGTATGGATCTTCAGGCTTGTATAGATCTCATCGAGAAACCGATGGGG

ATTATGAGTATTCTGGAGGAGGAATGTATGTTCCCGAAAGCCACTGACATGACAT

TTAAGGCCAAGCTGTTCGATAATCACTTGGGGAAGTCCGCCAATTTCCAGAAACC

TAGGAACATAAAAGGTAAGCCGGAGGCGCACTTCTCTCTTATCCATTACGCGGG

AATCGTCGATTATAACATTATTGGCTGGCTTCAAAAGAACAAGGATCCGCTTAAC

GAAACAGTGGTCGGCCTTTATCAGAAAAGCTCACTGAAACTTCTTAGTACGCTCT

TTGCTAATTACGCTGGTGCTGACGCTCCAATCGAGAAAGGCAAGGGAAAAGCTA

AGAAAGGCAGTAGCTTTCAAACAGTCTCTGCCCTGCACAGAGAGAATCTCAACA

AGCTCATGACCAATCTGCGGAGCACACATCCACATTTTGTCAGGTGCATAATCCC

TAATGAGACGAAAAGTCCAGGCGTAATGGACAATCCGCTGGTTATGCACCAGCT

C AGATGT AAT GGAGT ATT GGAAGGT AT ACGC AT AT GT AGAAAAGGCTTCCC AAA

CAGGATACTGTATGGTGACTTCCGACAACGCTATCGAATACTGAACCCCGCAGCC

ATTCCCGAGGGACAATTCATAGACAGCCGCAAGGGAGCGGAAAAGCTTCTTTCC

TCCCTCGACATAGACCATAACCAGTACAAATTTGGGCACACCAAAGTATTTTTCA

AAGCGGGCCTTCTTGGACTGTTGGAAGAGATGCGCGATGAAAGGCTGTCAAGGA

TAATAACCAGGATTCAAGCGCAATCTCGGGGAGTGCTCGCGAGGATGGAGTACA

AGAAATTGTT GGAACGGAGAGATTCCTTGCTT GT GATT C AGT GGAAT AT ACGAGC

TTTCATGGGAGTAAAAAACTGGCCTTGGATGAAACTCTACTTTAAGATAAAGCCA

CTGCTGAAGTCCGCAGAGAGGGAGAAGGAGATGGCATCAATGAAGGAGGAATT

T ACGCGCTT GAAAGAGGCTCTCGAGAAGTCCGAAGCT AGACGGAAGGAATTGGA

AGAGA AG AT GGT AT C TC TTTT GC AGGAGA AGA AT GAT C TT C AGTTGC A AGT AC A

AGC GGA AC A AGAC A ATCTC GCC GACGC AGAGGA A AGGT GT G ATC AGTT GAT C A A

AAATAAAATCCAACTGGAAGCCAAGGTGAAAGAAATGAATGAGCGCTTGGAGG

ACGAAGAAGAGATGAATGCAGAGTTGACTGCGAAGAAGCGGAAGCTCGAAGAC

GAATGCAGCGAGCTCAAGCGCGACATTGACGACCTGGAATTGACCCTTGCCAAA

GTGGAAAAGGAGAAACATGCAACCGAGAATAAGGTAAAGAATCTGACAGAAGA

GATGGCGGGACTTGACGAGATTATCGCGAAGCTTACAAAGGAGAAGAAGGCCCT

TCAGGAAGCGCACCAACAGGCCCTTGACGATCTTCAGGCCGAGGAAGACAAAGT

GAACACATTGACTAAAGCTAAAGTGAAGCTTGAGCAACAGGTCGATGACGAATT

GCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTAAAGCAA

GTAAAACCTCTACAAATGTGGTAAAATCCGATAAGGGACTAGAGCATGGCTACG

TAGATAAGTAGCATGGCGGGTTAATCATTAACTACAAGGAACCCCTAGTGATGG AGTTGGCCACTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGGGCGACCAAA

GGTCGCCCGACGCCCGGGCTTTGCCCGGGCGGCCTCAGTGAGCGAGCGAGCGCG

CCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGC

GC AGCCTGAAT GGCGAAT GGAATTCC AGACGATT GAGCGT C AA AATGT AGGT AT

TTCCATGAGCGTTTTTCCGTTGCAATGGCTGGCGGTAATATTGTTCTGGATATTAC

CAGCAAGGCCGATAGTTTGAGTTCTTCTACTCAGGCAAGTGATGTTATTACTAAT

CAAAGAAGTATTGCGACAACGGTTAATTTGCGTGATGGACAGACTCTTTTACTCG

GTGGCCTCACTGATTATAAAAACACTTCTCAGGATTCTGGCGTACCGTTCCTGTC

TAAAATCCCTTTAATCGGCCTCCTGTTTAGCTCCCGCTCTGATTCTAACGAGGAA

AGCACGTTATACGTGCTCGTCAAAGCAACCATAGTACGCGCCCTGTAGCGGCGC

ATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAG

CGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCG

GCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCT

TTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGC

CATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAAT

AGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTT

TGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATT

TAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTCTACAATTTAAATAT

TTGCTTATACAATCTTCCTGTTTTTGGGGCTTTTCTGATTATCAACCGGGGTACAT

ATGATTGACATGCTAGTTTTACGATTACCGTTCATCGATTCTCTTGTTTGCTCCAG

ACTCTCAGGCAATGACCTGATAGCCTTTGTAGAGACCTCTCAAAAATAGCTACCC

TCTCCGGCATGAATTTATCAGCTAGAACGGTTGAATATCATATTGATGGTGATTT

GACTGTCTCCGGCCTTTCTCACCCGTTTGAATCTTTACCTACACATTACTCAGGCA

TTGCATTTAAAATATATGAGGGTTCTAAAAATTTTTATCCTTGCGTTGAAATAAA

GGCTTCTCCCGCAAAAGTATTACAGGGTCATAATGTTTTTGGTACAACCGATTTA

GCTTTATGCTCTGAGGCTTTATTGCTTAATTTTGCTAATTCTTTGCCTTGCCTGTAT

GATTTATTGGATGTTGGAATCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGC

GGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCAT

AGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTT

GTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCAT

GTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGT

GATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAG

GTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATA

CATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAAT ATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTT

TTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAA

AAGAT GCTGAAGATC AGTT GGGT GC ACGAGT GGGTT AC ATCGAACTGGATCTC A

ACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAG

CACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAA

GAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCAC

C AGT C AC AGA A A AGC AT C TT ACGGAT GGC AT GAC AGT A AG AG A ATT AT GC AGT G

CTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGG

AGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCG

CCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGA

CACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGA

ACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAA

AGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGAT

AAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA

GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACT

ATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCAT

TGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCA

TTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAA

ATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCA

AAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAA

AAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTT

TTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGT

GTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTC

GCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTA

CCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAA

CGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGA

GATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGG

CGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAG

CTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCT

GACTT GAGCGTCGATTTTTGT GAT GCTCGT C AGGGGGGCGGAGCCT ATGGA AAA

ACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCAC

ATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGA

GTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAG

CGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCC GATTCATTAATGCAGCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGC

CCGGGCGTCGGGCGACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCA

GAGAGGGAGTGGCCAACTCCATCACTAGGGGTTCCTTGTAGTTAATGATTAACCC

GCCATGCTACTTATCTACGTAGCCATGCTCTAGATGTCATGGAGAAGACCCACCT

TGCAGATGTCCTCACTGGGGCTGGCAGAGCCGGCAACCTGCCTAAGGCTGCTCA

GTCCATTAGGAGCCAGTAGCCTGGAAGATGTCTTTACCCCCAGCATCAGTTCAAG

TGGAGCAGCACATAACTCTTGCCCTCTGCCTTCCAAGATTCTGGTGCTGAGACTT

ATGGAGTGTCTTGGAGGTTGCCTTCTGCCCCCCAACCCTGCTCCCAGCTGGCCCT

CCCAGGCCTGGGTTGCTGGCCTCTGCTTTATCAGGATTCTCAAGAGGGACAGCTG

GTTTATGTTGCATGACTGTTCCCTGCATATCTGCTCTGGTTTTAAATAGCTTATCT

GAGCAGCTGGAGGACCACATGGGCTTATATGGCGTGGGGTACATGTTCCTGTAG

CCTTGTCCCTGGCACCTGCCAAAATAGCAGCCAACACCCCCCACCCCCACCGCCA

TCCCCCTGCCCCACCCGTCCCCTGTCGCACATTCCTCCCTCCGCAGGGCTGGCTCA

CCAGGCCCCAGCCCACATGCCTGCTTAAAGCCCTCTCCATCCTCTGCCTCACCCA

GTCCCCGCTGAGACTGAGCAGACGCCTCCAGAGCTCGGATCCTGAGAACTTCAG

GGT G AGT C T AT GGG AC

[0117] SEQ ID NO: 7 below is an AAV vector encoding a second portion of MYH7 that includes a homologous overlapping sequence:

[0118] GGCCGCTTCCCTTTAGTGAGGGTTAATGCTTCGAGCAGACATGATAAG

AT AC ATTGATGAGTTT GGAC AAACC AC AACT AGAAT GC AGT GAAAAAAAT GCTT

TATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATA

AACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGAT

GTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCCGATA

AGGGACTAGAGCATGGCTACGTAGATAAGTAGCATGGCGGGTTAATCATTAACT

ACAAGGAACCCCTAGTGATGGAGTTGGCCACTCCCTCTCTGCGCGCTCGCTCGCT

CACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGGGCGGC

CTCAGTGAGCGAGCGAGCGCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCG

ATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGAATTCCAGACGATT

GAGCGTCAAAATGTAGGTATTTCCATGAGCGTTTTTCCGTTGCAATGGCTGGCGG

TAATATTGTTCTGGATATTACCAGCAAGGCCGATAGTTTGAGTTCTTCTACTCAG

GCAAGTGATGTTATTACTAATCAAAGAAGTATTGCGACAACGGTTAATTTGCGTG

ATGGACAGACTCTTTTACTCGGTGGCCTCACTGATTATAAAAACACTTCTCAGGA

TTCTGGCGTACCGTTCCTGTCTAAAATCCCTTTAATCGGCCTCCTGTTTAGCTCCC

GCTCTGATTCTAACGAGGAAAGCACGTTATACGTGCTCGTCAAAGCAACCATAGT

ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCG

TGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCC

TTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTT

AGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGT

GATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGT

TGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAA

CCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATT

GGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATT

AACGTCTACAATTTAAATATTTGCTTATACAATCTTCCTGTTTTTGGGGCTTTTCT

GATT AT C AACCGGGGT AC AT AT GATT GAC AT GCT AGTTTT ACGATT ACCGTTC AT

CGATTCTCTTGTTTGCTCCAGACTCTCAGGCAATGACCTGATAGCCTTTGTAGAG

ACCTCTCAAAAATAGCTACCCTCTCCGGCATGAATTTATCAGCTAGAACGGTTGA

ATATCATATTGATGGTGATTTGACTGTCTCCGGCCTTTCTCACCCGTTTGAATCTT

TACCTACACATTACTCAGGCATTGCATTTAAAATATATGAGGGTTCTAAAAATTT

TTATCCTTGCGTTGAAATAAAGGCTTCTCCCGCAAAAGTATTACAGGGTCATAAT

_{GTTTTTGGTACAACCGATTTAGCTTTATGCTCTGAGGCTTTATTGCTTAATTTTG C} TAATTCTTTGCCTTGCCTGTATGATTTATTGGATGTTGGAATCGCCTGATGCGGTA

TTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTA

CAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGC

TGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTG

ACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCG

CGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAAT

AAT GGTTTCTT AGACGT C AGGT GGC ACTTTTCGGGGAAAT GT GCGCGGA ACCCCT

ATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACC

CTGAT AAAT GCTTC A AT AAT ATTGAA AAAGGAAGAGT AT GAGT ATT C AAC ATTTC

CGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCA

G A A AC GC T GGT G A A AGT A A A AG AT GC T G A AG AT C AGTT GGGT GC AC GAGT GGGT

TACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAG

AACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCC

CGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAAT

GACTT GGTT GAGT ACTC ACC AGT C AC AGAAAAGC ATCTT ACGGAT GGC AT GAC A

GTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAAC

TTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACA

T GGGGGAT CAT GT A ACTC GCC TT GAT C GTT GGGA AC CGGAGC T GA AT GA AGC C A

TACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGC

GCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGA

CTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCT

GGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCA

TTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGAC

GGGGAGT C AGGC A AC T AT GGAT GA ACGA A AT AGAC AGATCGCTGAGAT AGGT GC

CTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAG

ATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGA

TAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGAC

CCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCT

GCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCA

AGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCA

AATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAG

CACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGG

CGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGC

GCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAAC GACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCT

TCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAG

GAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTG

TCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGG

GCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTT

TGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAA

CCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAG

CGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCC

TCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGCGCGCTCGCTCGCTCACTG

AGGCCGCCCGGGCAAAGCCCGGGCGTCGGGCGACCTTTGGTCGCCCGGCCTCAG

TGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCCATCACTAGGGGTTCCT

TGTAGTTAATGATTAACCCGCCATGCTACTTATCTACGTAGCCATGCTCTAGAGC

GTAATGGACAATCCGCTGGTTATGCACCAGCTCAGATGTAATGGAGTATTGGAA

GGTATACGCATATGTAGAAAAGGCTTCCCAAACAGGATACTGTATGGTGACTTCC

GACAACGCTATCGAATACTGAACCCCGCAGCCATTCCCGAGGGACAATTCATAG

ACAGCCGCAAGGGAGCGGAAAAGCTTCTTTCCTCCCTCGACATAGACCATAACC

AGTACAAATTTGGGCACACCAAAGTATTTTTCAAAGCGGGCCTTCTTGGACTGTT

GGA AGAG AT GC GCGAT GA A AGGCTGT C A AGGAT A AT A ACC AGG ATT C A AGC GC

AATCTCGGGGAGT GCTCGCGAGGATGGAGT AC AAGAAATT GTT GGAACGGAGAG

ATTCCTTGCTTGTGATTCAGTGGAATATACGAGCTTTCATGGGAGTAAAAAACTG

GCCTT GGAT GAAACTCT ACTTT AAGAT AAAGCC ACTGCTGAAGTCCGC AGAGAG

GG AG A AGG AG AT GGC AT C A AT GA AGGAGG A ATTT AC GCGC TT GA A AGAGGCTC T

CGAGAAGTCCGAAGCTAGACGGAAGGAATTGGAAGAGAAGATGGTATCTCTTTT

GCAGGAGAAGAATGATCTTCAGTTGCAAGTACAAGCGGAACAAGACAATCTCGC

CGACGC AGAGGA A AGGT GT GAT C AGTT GATC A A A A AT A A A ATCC A AC T GGA AGC

CAAGGTGAAAGAAATGAATGAGCGCTTGGAGGACGAAGAAGAGATGAATGCAG

AGTTGACTGCGAAGAAGCGGAAGCTCGAAGACGAATGCAGCGAGCTCAAGCGC

GACATTGACGACCTGGAATTGACCCTTGCCAAAGTGGAAAAGGAGAAACATGCA

ACCGAGAATAAGGTAAAGAATCTGACAGAAGAGATGGCGGGACTTGACGAGAT

T AT C GCGA AGC TT AC A A AGGAGA AGA AGGC CCTTC AGGA AGC GC AC C A AC AGGC

CCTTGACGATCTTCAGGCCGAGGAAGACAAAGTGAACACATTGACTAAAGCTAA

AGT GAAGCTTGAGC AAC AGGTCGAT GACCTGGAAGGCTC ACTTGAGC AAGAGAA

GAAAGTGCGCATGGATCTCGAGCGCGCTAAACGCAAGCTCGAGGGAGATTTGAA

GCTGAC T C A AGAGTC A ATT AT GGACC TT GA A A AC GAC A AGC A AC AGTT GGAT GA GAG AC TT A AG A AG A AGG AC TTCGA AC T C A ACGC AC TT A AT GC C AGGATCGA AG A

TGAACAAGCTCTCGGGTCTCAGCTCCAAAAGAAGCTTAAGGAACTCCAGGCACG

CATAGAAGAACTCGAAGAAGAGTTGGAAGCCGAGAGAACGGCGAGAGCTAAAG

TGGAAAAACTCCGAAGCGATTTGTCCAGAGAGCTGGAAGAGATTTCCGAGCGGC

TTGAGGAGGCGGGAGGCGCGACGAGCGTGCAGATCGAAATGAATAAGAAGAGA

GAAGCTGAATTTCAAAAAATGCGCCGAGACCTTGAAGAAGCAACACTCCAGCAC

GAAGCCACAGCAGCTGCGCTTCGGAAGAAGCATGCTGATTCTGTCGCAGAGCTG

GGGGAGC AGATCGAC AATCTCC AGCGAGT C AAAC AAAAACTGGAAAAGGAGAA

ATCTGAGTTTAAATTGGAGCTTGACGACGTCACGTCTAACATGGAGCAGATAATA

AAGGCAAAAGCAAACCTTGAGAAAATGTGCCGCACTCTTGAGGACCAGATGAAC

GAGCATCGCAGTAAGGCGGAAGAGACTCAGAGATCTGTTAACGACTTGACCAGT

CAACGAGCTAAACTTCAGACAGAAAATGGAGAACTCTCCCGACAACTGGACGAA

AAGGAGGCCCTGATCAGCCAGTTGACTAGGGGTAAACTCACCTACACACAGCAG

CTTGAGGACTTGAAGCGCCAACTCGAAGAGGAGGTGAAAGCTAAAAACGCACTG

GCACATGCGCTGCAATCCGCAAGACATGACTGCGACCTCCTGCGGGAACAGTAC

GAAGAAGAAACTGAGGCGAAAGCTGAGCTCCAACGGGTACTTTCTAAGGCAAAT

TCTGAAGTTGCACAGTGGCGAACTAAGTACGAAACCGATGCGATCCAACGCACG

GAGGAGTTGGAGGAAGCTAAAAAGAAGCTCGCACAGCGACTGCAGGAAGCCGA

AGA AGC CGTTGAGGC AGTT A AT GCA A A AT GC AGT AGT C TC GAGA AG AC T A A AC A

TAGACTCCAGAACGAAATAGAGGATCTGATGGTGGATGTTGAACGCTCCAATGC

TGCTGCGGCAGCCCTCGACAAAAAACAGCGGAATTTCGATAAGATTCTTGCGGA

AT GGA AGC AG A A AT AC GA AGAGTCTC AGAGT GA ACTGGAGAGC T C CCA A A AGG

AGGCCCGAAGTCTGTCTACCGAATTGTTTAAACTCAAGAACGCGTACGAAGAAT

CTCTGGAAC ACTT GGAAACCTTT AAAAGAGAGAAC AAAAACCTGC AAGAGGA AA

TAAGTGACCTTACCGAGCAACTGGGGAGCTCCGGGAAAACCATCCACGAATTGG

AAAAAGTCAGGAAACAGTTGGAGGCAGAGAAAATGGAGCTCCAATCCGCCCTG

GAGGAAGCGGAAGCATCTCTGGAACACGAGGAGGGCAAGATTCTGAGGGCCCA

ACTGGAATTT AACC AGATC AAGGC AGAGAT AGAACGAAAACTCGCGGAGAAGG

ACGAAGAGATGGAGCAGGCGAAACGGAATCATCTGCGGGTTGTAGACTCCCTGC

AAACCTCCCTCGATGCCGAAACCAGAAGCCGGAATGAGGCCCTTCGGGTGAAAA

AAAAAATGGAGGGCGACTTGAACGAAATGGAAATTCAACTTTCTCACGCCAACC

GCATGGCGGCCGAGGCGCAGAAACAGGTAAAATCTCTCCAGTCTCTCCTCAAAG

AT AC AC AAATCC A ACTCGACGATGCTGT AAGGGC AAAC GAT GATTT GAAAGAGA

ATATAGCAATCGTCGAGCGCCGCAATAATCTGTTGCAAGCAGAGCTTGAAGAAC TGCGCGCGGTCGTAGAACAGACCGAACGCAGTAGAAAGTTGGCTGAGCAGGAAC

TTATTGAGACTTCCGAGCGCGTTCAGCTTCTGCATTCCCAGAACACCTCTCTGATT

A AT C AGA AGA A A A AGAT GGAC GC AGATCTGT C TC AGTT GC AGACGGA AGT GGA A

GAAGCTGTTCAAGAGTGCCGGAACGCGGAGGAAAAAGCTAAAAAGGCGATAAC

T GATGC AGCGAT GAT GGCTGAGGAACTGAAGAAGGAGC AAGAC ACCTCCGCCC A

TTTGGAGCGAATGAAGAAGAATATGGAACAAACTATTAAGGATCTCCAGCACCG

GCTTGATGAGGCTGAACAGATCGCCTTGAAAGGGGGGAAAAAGCAATTGCAGAA

ATTGGAAGCCCGAGTAAGGGAACTGGAGAATGAGTTGGAAGCTGAGCAAAAGC

GGA AC GC T G AGT C T GT G A AGGG A AT GAG A A AG AGT G A AC GG AGG AT A A A AG A A

CTCACATATCAAACGGAAGAGGACCGGAAAAACTTGCTCCGCCTCCAAGACCTC

GTTGACAAGCTTCAATTGAAAGTCAAGGCTTATAAAAGACAAGCTGAAGAAGCT

GAAGAACAAGCGAACACCAATTTGTCCAAATTTCGGAAAGTACAGCATGAGCTC

GAC GAGGCTGAGGAGC GGGCTGAC AT AGC T GAGTCC C AGGT C A AT A A AC T GC GA

GCGA AAAGC AGAGAC ATT GGC ACC AAAGGTTT GAAT GAAGAGACCGGT GAGGG

CAGAGGAAGTCTTCTAACATGCGGTGACGTGGAGGAGAATCCCGGCCCTTCCGG

TATGAAAACCTTCAACATCTCTCAGCAGGATCTGGAGCTGGTGGAGGTCGCCACT

GAGAAGATCACCATGCTCTATGAGGACAACAAGCACCATGTCGGGGCGGCCATC

AGGACCAAGACTGGGGAGATCATCTCTGCTGTCCACATTGAAGCCTACATTGGC

AGGGTCACTGTCTGTGCTGAAGCCATTGCCATTGGGTCTGCTGTGAGCAACGGGC

AGAAGGACTTTGACACCATTGTGGCTGTCAGGCACCCCTACTCTGATGAGGTGGA

CAGATCCATCAGGGTGGTCAGCCCCTGTGGCATGTGTAGAGAGCTGATCTCTGAC

TATGCTCCTGACTGCTTTGTGCTCATTGAGATGAATGGCAAGCTGGTCAAAACCA

CCATTGAGGAACTCATCCCCCTCAAGTACACCAGGAACTAATAAGC

[0119] SEQ ID NO: 8 below is an AAV vector encoding a first portion of MYH7 and an N-intein sequence:

GGC ACTGGGC AGGT A AGT ATC A AGGTT AC A AG AC AGGTTT A AGGAGAC C A AT AG

AAACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTG

GTCTTACTGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAAT

TACAGCTCTTAAGGCTAGAGTACTTAATACGACTCACTATAGGCTAGCGGTACCG

GTCGCCACCATGATGGCCAAACTCACTTCTGCAGTCCCAGTCCTCACAGCAAGGG

ATGTTGCAGGGGCTGTAGAGTTCTGGACTGACAGATTAGGATTCTCCAGAGACTT

TGTTGAAGATGATTTTGCTGGTGTTGTCAGAGATGATGTCACCCTCTTCATCTCAG

CAGTTCAGGACCAAGTTGTCCCTGACAACACCCTTGCTTGGGTCTGGGTCAGAGG

CCTAGATGAGCTTTATGCAGAATGGTCAGAAGTAGTCAGCACAAATTTCAGGGA

TGCCTCTGGCCCAGCCATGACAGAAATTGGTGAACAACCTTGGGGAAGGGAATT

TGCCCTCAGAGACCCTGCTGGAAATTGTGTCCATTTTGTAGCTGAGGAACAGGAC

GGATCTGGAGCAACAAACTTCTCACTACTCAAACAAGCAGGTGACGTGGAGGAG

AATCCCGGGCCTGACTACAAAGACCATGACGGTGATTATAAAGATCATGACATC

GATTACAAGGATGACGATGACAAGCTTGAGCTCGGATCCATGGGAGATTCTGAA

ATGGCCGTCTTCGGCGCTGCCGCACCATATCTCCGCAAAAGTGAAAAGGAGCGC

CTGGAGGCCCAAACCAGACCCTTCGATCTGAAAAAGGATGTGTTTGTCCCGGAT

G AC A AGC A AG A AT T T GT A A A AGC A A AG AT C GT C TC TC GGG AGGGC GGT A A AGT G

ACTGCTGAAACAGAATACGGCAAAACCGTCACTGTCAAAGAAGATCAGGTAATG

CAACAAAATCCTCCGAAATTTGATAAAATCGAAGACATGGCCATGTTGACGTTTT

T GC AT GAGCC AGCTGTTTT GT AT AATCTGAAGGATCGGT ACGGATCCTGGAT GAT

TTACACATACTCAGGCTTGTTCTGCGTGACCGTTAATCCCTATAAGTGGCTGCCT

GTCTACACGCCGGAGGTTGTTGCAGCATATAGGGGGAAAAAGAGGTCCGAAGCC

CCTCCCCACATCTTCTCCATTTCTGACAACGCCTACCAGTATATGCTGACTGACA

GGGAGA ACC AA AGC ATTCTT ATT ACCGGAGAGTCTGGGGCGGGC AAGACGGT AA

ATACAAAACGCGTGATACAGTACTTTGCAGTTATTGCTGCGATTGGCGACAGGA

GTAAAAAGGATCAGAGCCCCGGCAAGGGTACTCTCGAAGATCAAATTATCCAGG

CTAATCCCGCCCTCGAAGCGTTCGGGAATGCCAAAACGGTCCGCAATGATAACA

GTAGCAGATTTGGGAAGTTTATCCGCATCCATTTCGGAGCGACTGGCAAACTCGC

CTCAGCAGACATCGAGACATACCTGTTGGAAAAGTCACGAGTGATATTTCAGCTC

AAAGCCGAGCGGGACTATCATATCTTTTACCAGATACTTTCTAACAAAAAACCAG

AGCTTCTTGACATGCTTCTGATCACTAACAATCCGTATGACTATGCATTCATCAGT

CAGGGAGAGACCACGGTTGCGTCAATAGACGATGCTGAGGAGCTGATGGCCACA GACAATGCCTTCGATGTGCTCGGATTTACTAGCGAGGAGAAGAACAGCATGTAC

AAGCTTACCGGTGCTATCATGCACTTCGGCAATATGAAATTTAAACTCAAGCAGC

GGG A AG A AC AGGC GG AGC C GG AC GG A AC T G A AG AGGC T GAT A A A AGT GC C T AC

CTCATGGGGCTTAACTCTGCCGACTTGCTTAAAGGATTGTGTCATCCACGGGTAA

AGGT AGGT A AT GA AT AT GT A ACT A AGGGT C A A A AT GT GCA AC A AGT CAT AT AC G

CGACTGGGGCTCTGGCAAAAGCCGTTTACGAGAGAATGTTTAACTGGATGGTCA

CACGAATTAATGCCACTCTGGAGACAAAACAACCCCGGCAGTACTTTATAGGTG

TGCTGGATATCGCAGGTTTCGAAATTTTCGATTTCAACTCTTTCGAGCAACTTTGT

ATCAATTTCACCAACGAGAAGCTCCAACAATTTTTTAACCATCATATGTTCGTTCT

CGAGCAAGAGGAATACAAAAAAGAGGGCATCGAATGGACTTTCATCGATTTCGG

TATGGATCTTCAGGCTTGTATAGATCTCATCGAGAAACCGATGGGGATTATGAGT

ATTCTGGAGGAGGAATGTATGTTCCCGAAAGCCACTGACATGACATTTAAGGCC

AAGCTGTTCGATAATCACTTGGGGAAGTCCGCCAATTTCCAGAAACCTAGGAAC

ATAAAAGGTAAGCCGGAGGCGCACTTCTCTCTTATCCATTACGCGGGAATCGTCG

ATTATAACATTATTGGCTGGCTTCAAAAGAACAAGGATCCGCTTAACGAAACAG

TGGTCGGCCTTTATCAGAAAAGCTCACTGAAACTTCTTAGTACGCTCTTTGCTAA

TTACGCTGGTGCTGACGCTCCAATCGAGAAAGGCAAGGGAAAAGCTAAGAAAGG

CAGTAGCTTTCAAACAGTCTCTGCCCTGCACAGAGAGAATCTCAACAAGCTCATG

ACCAATCTGCGGAGCACACATCCACATTTTGTCAGGTGCATAATCCCTAATGAGA

CGAAAAGTCCAGGCGTAATGGACAATCCGCTGGTTATGCACCAGCTCAGATGTA

AT GGAGT ATTGGAAGGT AT ACGC AT AT GT AGAAAAGGCTTCCC A AAC AGGAT AC

TGTATGGTGACTTCCGACAACGCTATCGAATACTGAACCCCGCAGCCATTCCCGA

GGGACAATTCATAGACAGCCGCAAGGGAGCGGAAAAGCTTCTTTCCTCCCTCGA

CATAGACCATAACCAGTACAAATTTGGGCACACCAAAGTATTTTTCAAAGCGGG

CCTTCTTGGACTGTTGGAAGAGATGCGCGATGAAAGGCTGTCAAGGATAATAAC

C AGGATT C AAGCGC AATCTCGGGGAGT GCTCGCGAGGATGGAGT AC AAGAAATT

GTTGGAACGGAGAGATTCCTTGCTTGTGATTCAGTGGAATATACGAGCTTTCATG

GGAGT AAAAAACTGGCCTTGGATGAAACTCTACTTTAAGATAAAGCCACTGCTG

AAGTCCGCAGAGAGGGAGAAGGAGATGGCATCAATGAAGGAGGAATTTACGCG

CTTGAAAGAGGCTCTCGAGAAGTCCGAAGCTAGACGGAAGGAATTGGAAGAGA

AGATGGTATCTCTTTTGCAGGAGAAGAATGATCTTCAGTTGCAAGTACAAGCGGA

ACAAGACAATCTCGCCGACGCAGAGGAAAGGTGTGATCAGTTGATCAAAAATAA

A AT C C A AC T GG A AGC C A AGGT G A A AG A A AT G A AT G AGC GC TT GG AGG AC G AAG

AAGAGATGAAT GC AGAGTT GACTGCGA AGAAGCGGAAGCTCGAAGACGAAT GC CTGTCCTACGAGACCGAAATCCTGACCGTGGAGTATGGGCTGCTGCCCATCGGCA

AGATTGTGGAGAAGCGGATTGAATGCACCGTGTATAGCGTGGACAACAACGGCA

ACATCTACACCCAGCCCGTGGCTCAGTGGCACGACAGGGGCGAGCAGGAGGTGT

TTGAGTATTGTCTGGAGGACGGCAGCCTGATTAGAGCCACCAAAGACCACAAGT

T C ATGACCGT GGACGGGC AGAT GCTGCCC ATT GACGAGATTTTT GAGCGGGAAC

TTGACCTGATGCGGGTGGACAACCTGCCCAACTGAACGCGTGCGGCCGCTTCCCT

TTAGTGAGGGTTAATGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGA

CAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATG

CTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAA

TTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTAAAGC

AAGTAAAACCTCTACAAATGTGGTAAAATCCGATAAGGGACTAGAGCATGGCTA

CGTAGATAAGTAGCATGGCGGGTTAATCATTAACTACAAGGAACCCCTAGTGAT

GGAGTTGGCCACTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGGGCGACCA

AAGGTCGCCCGACGCCCGGGCTTTGCCCGGGCGGCCTCAGTGAGCGAGCGAGCG

CGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTT

GCGC AGCCTGAAT GGCGAAT GGAATTCC AGACGATT GAGCGT C AAAAT GT AGGT

ATTTCCATGAGCGTTTTTCCGTTGCAATGGCTGGCGGTAATATTGTTCTGGATATT

ACCAGCAAGGCCGATAGTTTGAGTTCTTCTACTCAGGCAAGTGATGTTATTACTA

ATCAAAGAAGTATTGCGACAACGGTTAATTTGCGTGATGGACAGACTCTTTTACT

CGGTGGCCTCACTGATTATAAAAACACTTCTCAGGATTCTGGCGTACCGTTCCTG

TCTAAAATCCCTTTAATCGGCCTCCTGTTTAGCTCCCGCTCTGATTCTAACGAGGA

AAGCACGTTATACGTGCTCGTCAAAGCAACCATAGTACGCGCCCTGTAGCGGCG

CATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCA

GCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCC

GGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTG

CTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGG

GCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTA

ATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTC

TTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTG

ATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTCTACAATTTAAA

TATTTGCTTATACAATCTTCCTGTTTTTGGGGCTTTTCTGATTATCAACCGGGGTA

CATATGATTGACATGCTAGTTTTACGATTACCGTTCATCGATTCTCTTGTTTGCTC

CAGACTCTCAGGCAATGACCTGATAGCCTTTGTAGAGACCTCTCAAAAATAGCTA

CCCTCTCCGGCATGAATTTATCAGCTAGAACGGTTGAATATCATATTGATGGTGA TTTGACTGTCTCCGGCCTTTCTCACCCGTTTGAATCTTTACCTACACATTACTCAG GCATTGCATTTAAAATATATGAGGGTTCTAAAAATTTTTATCCTTGCGTTGAAAT AAAGGCTTCTCCCGC AAAAGT ATT AC AGGGT CAT AAT GTTTTTGGT AC AACCGAT TTAGCTTTATGCTCTGAGGCTTTATTGCTTAATTTTGCTAATTCTTTGCCTTGCCTG TATGATTTATTGGATGTTGGAATCGCCTGATGCGGTATTTTCTCCTTACGCATCTG TGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCG CATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGG CTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTG CATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCT CGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGT CAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAA ATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAAT AATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCC _{CTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAA G} TAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATC TCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGAT GAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGG CAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACT C ACC AGT C AC AGAAAAGC ATCTT ACGGAT GGC AT GAC AGT AAGAGAATT ATGC A GTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGAT CGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAAC TCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCG TGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGG CGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGAT AAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTG AT AAATCTGGAGCCGGT GAGCGTGGGTCTCGCGGT ATC ATT GC AGC ACTGGGGC CAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAA C T AT GG AT G A AC G A A AT AG AC AG AT C GC T GAG AT AGGT GC C TC AC T GAT T A AGC ATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTT CATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCA AAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGAT CAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAA AAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCT TTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTA GTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACC

TCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCT

TACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTG

AACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACT

GAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAA

GGC GGAC AGGT ATCCGGT A AGC GGC AGGGT C GGA AC AGG AGAGCGC AC GAGGG

AGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCT

CTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA

AACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCA

CATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG

AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGA

GCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGC

CGATTCATTAATGCAGCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAG

CCCGGGCGTCGGGCGACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGC

AGAGAGGGAGTGGCCAACTCCATCACTAGGGGTTCCTTGTAGTTAATGATTAACC

CGCCATGCTACTTATCTACGTAGCCATGCTCTAGATGTCATGGAGAAGACCCACC

TTGCAGATGTCCTCACTGGGGCTGGCAGAGCCGGCAACCTGCCTAAGGCTGCTCA

GTCCATTAGGAGCCAGTAGCCTGGAAGATGTCTTTACCCCCAGCATCAGTTCAAG

TGGAGCAGCACATAACTCTTGCCCTCTGCCTTCCAAGATTCTGGTGCTGAGACTT

ATGGAGTGTCTTGGAGGTTGCCTTCTGCCCCCCAACCCTGCTCCCAGCTGGCCCT

CCCAGGCCTGGGTTGCTGGCCTCTGCTTTATCAGGATTCTCAAGAGGGACAGCTG

GTTTATGTTGCATGACTGTTCCCTGCATATCTGCTCTGGTTTTAAATAGCTTATCT

GAGC AGCTGGAGGACC AC AT GGGCTT AT AT GGCGT GGGGT AC AT GTTCCTGT AG

CCTTGTCCCTGGCACCTGCCAAAATAGCAGCCAACACCCCCCACCCCCACCGCCA

TCCCCCTGCCCCACCCGTCCCCTGTCGCACATTCCTCCCTCCGCAGGGCTGGCTCA

CCAGGCCCCAGCCCACATGCCTGCTTAAAGCCCTCTCCATCCTCTGCCTCACCCA

GTCCCCGCTGAGACTGAGCAGACGCCTCCAGAGCTCGGATCCTGAGAACTTCAG

GGT G AGT C T AT GGG AC [0120] SEQ ID NO: 9 below is an AAV vector encoding a second portion of MYH7 and a C-intein sequence:

GGC ACTGGGC AGGT A AGT ATC A AGGTT AC A AG AC AGGTTT A AGGAGAC C A AT AG

AAACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTG

GTCTTACTGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAAT

TACAGCTCTTAAGGCTAGAGTACTTAATACGACTCACTATAGGCTAGCGGTACCG

GTCGCCACCATGATGATAAAGATTGCCACCAGAAAGTATCTGGGCAAGCAGAAC

GTGTATGACATCGGCGTGGAGAGAGACCACAACTTCGCCCTGAAGAACGGCTTC

ATCGCCAGCAACTGCAGCGAGCTCAAGCGCGACATTGACGACCTGGAATTGACC

CTTGC C A A AGT GGA A A AGGAGA A AC AT GC A AC CGAGA AT A AGGT A A AG A AT C T

GACAGAAGAGATGGCGGGACTTGACGAGATTATCGCGAAGCTTACAAAGGAGA

AGAAGGCCCTTCAGGAAGCGCACCAACAGGCCCTTGACGATCTTCAGGCCGAGG

A AG AC A A AGT GA AC AC ATTGAC T A A AGCT A A AGT GA AGC TT GAGC A AC AGGT C G

ATGACCTGGAAGGCTCACTTGAGCAAGAGAAGAAAGTGCGCATGGATCTCGAGC

GCGCTAAACGCAAGCTCGAGGGAGATTTGAAGCTGACTCAAGAGTCAATTATGG

ACC TT GA A A AC GAC A AGC A AC AGTT GGAT GAG AG AC TT A AGA AGA AGG ACTTCG

AACTCAACGCACTTAATGCCAGGATCGAAGATGAACAAGCTCTCGGGTCTCAGC

TCCAAAAGAAGCTTAAGGAACTCCAGGCACGCATAGAAGAACTCGAAGAAGAG

TTGGAAGCCGAGAGAACGGCGAGAGCTAAAGTGGAAAAACTCCGAAGCGATTT

GTCCAGAGAGCTGGAAGAGATTTCCGAGCGGCTTGAGGAGGCGGGAGGCGCGA

CGAGCGTGCAGATCGAAATGAATAAGAAGAGAGAAGCTGAATTTCAAAAAATG

CGCCGAGACCTTGAAGAAGCAACACTCCAGCACGAAGCCACAGCAGCTGCGCTT

CGGAAGAAGCATGCTGATTCTGTCGCAGAGCTGGGGGAGCAGATCGACAATCTC

C AGCGAGT C AAAC AAAAACTGGAAAAGGAGAAATCTGAGTTT AAATT GGAGCTT

GAC GAC GT C ACGTCT AAC AT GGAGC AGAT AAT AAAGGC AAAAGC AAACCTTGAG

AAAAT GTGCCGC ACTCTT GAGGACC AGAT GAACGAGC ATCGC AGT AAGGCGGAA

GAGACTCAGAGATCTGTTAACGACTTGACCAGTCAACGAGCTAAACTTCAGACA

GAAAATGGAGAACTCTCCCGACAACTGGACGAAAAGGAGGCCCTGATCAGCCAG

TTGACTAGGGGTAAACTCACCTACACACAGCAGCTTGAGGACTTGAAGCGCCAA

CTCGAAGAGGAGGTGAAAGCT AAAAACGC ACTGGC AC ATGCGCTGC AATCCGC A

AGACATGACTGCGACCTCCTGCGGGAACAGTACGAAGAAGAAACTGAGGCGAA

AGCTGAGCTCCAACGGGTACTTTCTAAGGCAAATTCTGAAGTTGCACAGTGGCG

AACTAAGTACGAAACCGATGCGATCCAACGCACGGAGGAGTTGGAGGAAGCTA

AAAAGAAGCTCGCACAGCGACTGCAGGAAGCCGAAGAAGCCGTTGAGGCAGTT AATGCAAAATGCAGTAGTCTCGAGAAGACTAAACATAGACTCCAGAACGAAATA

GAGGATCTGATGGTGGATGTTGAACGCTCCAATGCTGCTGCGGCAGCCCTCGAC

A A A A A AC AGCGG A ATTTC GAT A AGATTCTTGC GGA AT GGA AGC AGA A AT AC GA A

GAGTCTCAGAGTGAACTGGAGAGCTCCCAAAAGGAGGCCCGAAGTCTGTCTACC

GAATTGTTTAAACTCAAGAACGCGTACGAAGAATCTCTGGAACACTTGGAAACC

TTTAAAAGAGAGAACAAAAACCTGCAAGAGGAAATAAGTGACCTTACCGAGCA

ACTGGGGAGC T C CGGGA A A AC C ATCC AC GA ATT GGA A A A AGT C AGGA A AC AGTT

GGAGGCAGAGAAAATGGAGCTCCAATCCGCCCTGGAGGAAGCGGAAGCATCTCT

GGAACACGAGGAGGGCAAGATTCTGAGGGCCCAACTGGAATTTAACCAGATCAA

GGC AGAGAT AGAACGA AAACTCGCGGAGAAGGACGAAGAGAT GGAGC AGGCGA

AACGGAATCATCTGCGGGTTGTAGACTCCCTGCAAACCTCCCTCGATGCCGAAAC

CAGAAGCCGGAATGAGGCCCTTCGGGTGAAAAAAAAAATGGAGGGCGACTTGA

ACGAAATGGAAATTCAACTTTCTCACGCCAACCGCATGGCGGCCGAGGCGCAGA

AACAGGTAAAATCTCTCCAGTCTCTCCTCAAAGATACACAAATCCAACTCGACGA

TGCTGTAAGGGCAAACGATGATTTGAAAGAGAATATAGCAATCGTCGAGCGCCG

CAATAATCTGTTGCAAGCAGAGCTTGAAGAACTGCGCGCGGTCGTAGAACAGAC

CGAACGCAGTAGAAAGTTGGCTGAGCAGGAACTTATTGAGACTTCCGAGCGCGT

TCAGCTTCTGCATTCCCAGAACACCTCTCTGATTAATCAGAAGAAAAAGATGGAC

GC AG ATCTGT C TC AGTT GC AGAC GGA AGT GGA AGA AGCTGTT C A AG AGT GCC GG

AACGCGGAGGAAAAAGCTAAAAAGGCGATAACTGATGCAGCGATGATGGCTGA

GGAACTGAAGAAGGAGCAAGACACCTCCGCCCATTTGGAGCGAATGAAGAAGA

ATATGGAACAAACTATTAAGGATCTCCAGCACCGGCTTGATGAGGCTGAACAGA

TCGCCTTGAAAGGGGGGAAAAAGCAATTGCAGAAATTGGAAGCCCGAGTAAGG

GAACTGGAGAATGAGTTGGAAGCTGAGCAAAAGCGGAACGCTGAGTCTGTGAA

GGG A AT GAG A A AG AGT G A AC GG AGG AT A A A AG A AC TCACATAT C A A AC GGA AG

AGGACCGGAAAAACTTGCTCCGCCTCCAAGACCTCGTTGACAAGCTTCAATTGA

A AGT C A AGGC TT AT A A A AGAC A AGC T GA AGA AGCTGA AGA AC A AGCGA AC AC C

AATTTGTCCAAATTTCGGAAAGTACAGCATGAGCTCGACGAGGCTGAGGAGCGG

GCTGAC AT AGCTGAGTCCC AGGT C AAT AAACTGCGAGCGAAAAGC AGAGAC ATT

GGCACCAAAGGTTTGAATGAAGAGACCGGTGAGGGCAGAGGAAGTCTTCTAACA

TGCGGTGACGTGGAGGAGAATCCCGGCCCTTCCGGTATGAAAACCTTCAACATCT

CTCAGCAGGATCTGGAGCTGGTGGAGGTCGCCACTGAGAAGATCACCATGCTCT

AT GAGGAC A AC A AGC ACC AT GT C GGGGCGGCC AT C AGG AC C A AGAC T GGGGAG

ATCATCTCTGCTGTCCACATTGAAGCCTACATTGGCAGGGTCACTGTCTGTGCTG AAGCCATTGCCATTGGGTCTGCTGTGAGCAACGGGCAGAAGGACTTTGACACCA

TTGTGGCTGTCAGGCACCCCTACTCTGATGAGGTGGACAGATCCATCAGGGTGGT

CAGCCCCTGTGGCATGTGTAGAGAGCTGATCTCTGACTATGCTCCTGACTGCTTT

GT GCTC ATT GAG AT GAATGGC AAGCTGGT C AAAACC ACC ATT GAGGAACTC AT C

CCCCTCAAGTACACCAGGAACTAATAAGCGGCCGCTTCCCTTTAGTGAGGGTTAA

TGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTA

GAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTT

GTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTA

TGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTA

C A A AT GT GGT A A A AT C C GAT A AGGG AC T AG AGC AT GGC T AC GT AG AT A AGT AGC

ATGGCGGGTTAATCATTAACTACAAGGAACCCCTAGTGATGGAGTTGGCCACTCC

CTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGC

CCGGGCTTTGCCCGGGCGGCCTCAGTGAGCGAGCGAGCGCGCCAGCTGGCGTAA

TAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGG

CGAATGGAATTCCAGACGATTGAGCGTCAAAATGTAGGTATTTCCATGAGCGTTT

TTCCGTT GC AAT GGCTGGCGGT AAT ATT GTTCTGGAT ATT ACC AGC AAGGCCGAT

AGTTTGAGTTCTTCTACTCAGGCAAGTGATGTTATTACTAATCAAAGAAGTATTG

CGACAACGGTTAATTTGCGTGATGGACAGACTCTTTTACTCGGTGGCCTCACTGA

TTATAAAAACACTTCTCAGGATTCTGGCGTACCGTTCCTGTCTAAAATCCCTTTAA

TCGGCCTCCTGTTTAGCTCCCGCTCTGATTCTAACGAGGAAAGCACGTTATACGT

GCTCGTCAAAGCAACCATAGTACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGG

GTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGC

TCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGC

TCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGAC

CCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAG

ACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTT

CCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGG

ATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTA

ACGCGAATTTTAACAAAATATTAACGTCTACAATTTAAATATTTGCTTATACAAT

CTTCCTGTTTTTGGGGCTTTTCTGATTATCAACCGGGGTACATATGATTGACATGC

TAGTTTTACGATTACCGTTCATCGATTCTCTTGTTTGCTCCAGACTCTCAGGCAAT

GACCTGATAGCCTTTGTAGAGACCTCTCAAAAATAGCTACCCTCTCCGGCATGAA

TTTATCAGCTAGAACGGTTGAATATCATATTGATGGTGATTTGACTGTCTCCGGC

CTTTCTCACCCGTTTGAATCTTTACCTACACATTACTCAGGCATTGCATTTAAAAT ATATGAGGGTTCTAAAAATTTTTATCCTTGCGTTGAAATAAAGGCTTCTCCCGCA

AAAGTATTACAGGGTCATAATGTTTTTGGTACAACCGATTTAGCTTTATGCTCTG

AGGCTTTATTGCTTAATTTTGCTAATTCTTTGCCTTGCCTGTATGATTTATTGGATG

TTGGAATCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACC

GCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCC

CCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCA

TCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTT

CACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTT

ATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGG

GGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTA

TCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAA

GAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTT

GCCTTCCTGTTTTT GCTC ACCC AGA AACGCTGGT GAAAGT AAAAGAT GCTGA AGA

T C AGTTGGGT GC ACGAGTGGGTT AC ATCGAACTGGATCTC AAC AGCGGT AAGAT

CCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTT

CTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTC

GCC GC AT AC AC T ATTC T C AGA AT GAC TT GGTTGAGT ACTC AC C AGT C AC AG A A A A

GCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCAT

GAGT GAT AAC ACTGCGGCC AACTT ACTTCTGAC AACGATCGGAGGACCGAAGGA

GCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGG

GAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCT

GTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAG

CTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCAC

TTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGG

TGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCC

CGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAAT

AGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAC

CAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAG

GATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAG

TTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAG

ATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACC

AGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACT

GGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAG

GCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCT GTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCA

AGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGC

ACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGT

GAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCC

GGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAA

ACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGA

TTTTT GTGAT GCTCGT C AGGGGGGCGGAGCCT AT GGAAAAACGCC AGC AACGCG

GCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCG

TTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCG

CTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAA

GAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCA

GCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCGGGC

GACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGC

CAACTCCATCACTAGGGGTTCCTTGTAGTTAATGATTAACCCGCCATGCTACTTA

TCTACGTAGCCATGCTCTAGATGTCATGGAGAAGACCCACCTTGCAGATGTCCTC

ACTGGGGCTGGCAGAGCCGGCAACCTGCCTAAGGCTGCTCAGTCCATTAGGAGC

CAGTAGCCTGGAAGATGTCTTTACCCCCAGCATCAGTTCAAGTGGAGCAGCACAT

AACTCTTGCCCTCTGCCTTCCAAGATTCTGGTGCTGAGACTTATGGAGTGTCTTGG

AGGTTGCCTTCTGCCCCCCAACCCTGCTCCCAGCTGGCCCTCCCAGGCCTGGGTT

GCTGGCCTCTGCTTTATCAGGATTCTCAAGAGGGACAGCTGGTTTATGTTGCATG

ACTGTTCCCTGCATATCTGCTCTGGTTTTAAATAGCTTATCTGAGCAGCTGGAGG

ACCACATGGGCTTATATGGCGTGGGGTACATGTTCCTGTAGCCTTGTCCCTGGCA

CCTGCCAAAATAGCAGCCAACACCCCCCACCCCCACCGCCATCCCCCTGCCCCAC

CCGTCCCCTGTCGCACATTCCTCCCTCCGCAGGGCTGGCTCACCAGGCCCCAGCC

CACATGCCTGCTTAAAGCCCTCTCCATCCTCTGCCTCACCCAGTCCCCGCTGAGA

CTGAGCAGACGCCTCCAGAGCTCGGATCCTGAGAACTTCAGGGTGAGTCTATGG

GAC [0121] SEQ ID NO: 10 below is an AAV vector encoding a first portion of MHY7 and including a recombinogenic exogenous sequence:

GGC ACTGGGC AGGT A AGT ATC A AGGTT AC A AG AC AGGTTT A AGGAGAC C A AT AG

AAACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTG

GTCTTACTGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAAT

TACAGCTCTTAAGGCTAGAGTACTTAATACGACTCACTATAGGCTAGCGGTACCG

GTCGCCACCATGGACTACAAAGACCATGACGGTGATTATAAAGATCATGACATC

GATT AC AAGGAT GACGATGAC AAGCTTGAGCTCGGATCC ATGGGAGATTCTGAA

ATGGCCGTCTTCGGCGCTGCCGCACCATATCTCCGCAAAAGTGAAAAGGAGCGC

CTGGAGGCCCAAACCAGACCCTTCGATCTGAAAAAGGATGTGTTTGTCCCGGAT

G AC A AGC A AG A AT T T GT A A A AGC A A AG AT C GT C TC TC GGG AGGGC GGT A A AGT G

ACTGCTGA A AC AGA AT AC GGC A A A AC CGT C ACTGT C A A AG A AG AT C AGGT A AT G

CAACAAAATCCTCCGAAATTTGATAAAATCGAAGACATGGCCATGTTGACGTTTT

TGCATGAGCCAGCTGTTTTGTATAATCTGAAGGATCGGTACGGATCCTGGATGAT

TTACACATACTCAGGCTTGTTCTGCGTGACCGTTAATCCCTATAAGTGGCTGCCT

GTCTACACGCCGGAGGTTGTTGCAGCATATAGGGGGAAAAAGAGGTCCGAAGCC

CCTCCCCACATCTTCTCCATTTCTGACAACGCCTACCAGTATATGCTGACTGACA

GGGAGA ACC AA AGC ATTCTT ATT ACCGGAGAGTCTGGGGCGGGC AAGACGGT AA

ATACAAAACGCGTGATACAGTACTTTGCAGTTATTGCTGCGATTGGCGACAGGA

GTAAAAAGGATCAGAGCCCCGGCAAGGGTACTCTCGAAGATCAAATTATCCAGG

CTAATCCCGCCCTCGAAGCGTTCGGGAATGCCAAAACGGTCCGCAATGATAACA

GTAGCAGATTTGGGAAGTTTATCCGCATCCATTTCGGAGCGACTGGCAAACTCGC

CTCAGCAGACATCGAGACATACCTGTTGGAAAAGTCACGAGTGATATTTCAGCTC

AAAGCCGAGCGGGACTATCATATCTTTTACCAGATACTTTCTAACAAAAAACCAG

AGCTTCTTGACATGCTTCTGATCACTAACAATCCGTATGACTATGCATTCATCAGT

CAGGGAGAGACCACGGTTGCGTCAATAGACGATGCTGAGGAGCTGATGGCCACA

GACAATGCCTTCGATGTGCTCGGATTTACTAGCGAGGAGAAGAACAGCATGTAC

AAGCTTACCGGTGCTATCATGCACTTCGGCAATATGAAATTTAAACTCAAGCAGC

GGG A AG A AC AGGC GG AGC C GG AC GG A AC T G A AG AGGC T GAT A A A AGT GC C T AC

CTCATGGGGCTTAACTCTGCCGACTTGCTTAAAGGATTGTGTCATCCACGGGTAA

AGGT AGGT A AT GA AT AT GT A AC T A AGGGTC A A A AT GT GCA AC A AGT CAT AT AC G

CGACTGGGGCTCTGGCAAAAGCCGTTTACGAGAGAATGTTTAACTGGATGGTCA

CACGAATTAATGCCACTCTGGAGACAAAACAACCCCGGCAGTACTTTATAGGTG

TGCTGGATATCGCAGGTTTCGAAATTTTCGATTTCAACTCTTTCGAGCAACTTTGT ATCAATTTCACCAACGAGAAGCTCCAACAATTTTTTAACCATCATATGTTCGTTCT

CGAGCAAGAGGAATACAAAAAAGAGGGCATCGAATGGACTTTCATCGATTTCGG

TATGGATCTTCAGGCTTGTATAGATCTCATCGAGAAACCGATGGGGATTATGAGT

ATTCTGGAGGAGGAATGTATGTTCCCGAAAGCCACTGACATGACATTTAAGGCC

AAGCTGTTCGATAATCACTTGGGGAAGTCCGCCAATTTCCAGAAACCTAGGAAC

ATAAAAGGTAAGCCGGAGGCGCACTTCTCTCTTATCCATTACGCGGGAATCGTCG

ATTATAACATTATTGGCTGGCTTCAAAAGAACAAGGATCCGCTTAACGAAACAG

TGGTCGGCCTTTATCAGAAAAGCTCACTGAAACTTCTTAGTACGCTCTTTGCTAA

TTACGCTGGTGCTGACGCTCCAATCGAGAAAGGCAAGGGAAAAGCTAAGAAAGG

CAGTAGCTTTCAAACAGTCTCTGCCCTGCACAGAGAGAATCTCAACAAGCTCATG

ACCAATCTGCGGAGCACACATCCACATTTTGTCAGGTGCATAATCCCTAATGAGA

CGAAAAGTCCAGGCGTAATGGACAATCCGCTGGTTATGCACCAGCTCAGATGTA

ATGGAGT ATT GGAAGGT AT ACGC AT AT GT AGAAAAGGCTTCCC A AAC AGGAT AC

TGTATGGTGACTTCCGACAACGGTATCGCATCCTGAACCCAGCGGCCATCCCTGA

GGGAC AGTT C ATTGAT AGC AGGAAGGGGGC AGAGA AGCTGCTC AGCTCCCTGGA

CATTGATCACAACCAGTACAAGTTTGGCCACACCAAGGTGAGTAAAGGAGACTA

ATTAATTAAAGGAAGACATCTCTGTGATCCTAGGTGGAGGCCGAAAGTACATGTT

TCGC AT GGGAACCCC AGACCCTGAGT ACCC AGAT GACT AC AGCC AAGGT GGGAC

C AGGCTGGACGGGAAGA ATCTGGT GC AGGAAT GGCTGGCGAAGCGCC AGGGT G

CCCGGTATGTGTGGAACCGCACTGAGCTCATGCAGGCTTCCCTGGACCCGTCTGT

GACCCATCTCATGGGTCTCTTTGAGCCTGGAGACATGAAATACGAGATCCACCGA

GACTCCACACTGGACCCCTCCCTGAATTGCATTCATTTTATGTTTCAGGTTCAGGG

GGAGAT GT GGGAGGTTTTTT A A AGC A AGT A A A AC CTC T AC A A AT GT GGT A A A AT

CCGATAAGGGACTAGAGCATGGCTACGTAGATAAGTAGCATGGCGGGTTAATCA

TTAACTACAAGGAACCCCTAGTGATGGAGTTGGCCACTCCCTCTCTGCGCGCTCG

CTCGCTCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCG

GGCGGCCTCAGTGAGCGAGCGAGCGCGCCAGCTGGCGTAATAGCGAAGAGGCCC

GCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGAATTCCA

GACGATTGAGCGTCAAAATGTAGGTATTTCCATGAGCGTTTTTCCGTTGCAATGG

CTGGCGGTAATATTGTTCTGGATATTACCAGCAAGGCCGATAGTTTGAGTTCTTC

TACTCAGGCAAGTGATGTTATTACTAATCAAAGAAGTATTGCGACAACGGTTAAT

TTGCGTGATGGACAGACTCTTTTACTCGGTGGCCTCACTGATTATAAAAACACTT

CTCAGGATTCTGGCGTACCGTTCCTGTCTAAAATCCCTTTAATCGGCCTCCTGTTT

AGCTCCCGCTCTGATTCTAACGAGGAAAGCACGTTATACGTGCTCGTCAAAGCAA CCATAGTACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACG

CGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTT

CCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGC

TCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGA

TTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCT

TTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAA

CACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCG

GCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACA

AAATATTAACGTCTACAATTTAAATATTTGCTTATACAATCTTCCTGTTTTTGGGG

CTTTTCTGATTATCAACCGGGGTACATATGATTGACATGCTAGTTTTACGATTACC

GTTCATCGATTCTCTTGTTTGCTCCAGACTCTCAGGCAATGACCTGATAGCCTTTG

TAGAGACCTCTCAAAAATAGCTACCCTCTCCGGCATGAATTTATCAGCTAGAACG

GTTGAATATCATATTGATGGTGATTTGACTGTCTCCGGCCTTTCTCACCCGTTTGA

ATCTTTACCTACACATTACTCAGGCATTGCATTTAAAATATATGAGGGTTCTAAA

AATTTTTATCCTTGCGTTGAAATAAAGGCTTCTCCCGCAAAAGTATTACAGGGTC

ATAATGTTTTTGGTACAACCGATTTAGCTTTATGCTCTGAGGCTTTATTGCTTAAT

TTTGCTAATTCTTTGCCTTGCCTGTATGATTTATTGGATGTTGGAATCGCCTGATG

CGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCT

CAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACA

CCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAG

CTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAA

ACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATG

ATAATAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAA

CCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAA

TAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAA

CATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCT

C AC CC AGA A ACGC T GGT GA A AGT A A A AG AT GC T GA AGATC AGTT GGGT GC AC GA

GTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCC

CCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGT

ATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCT

CAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGC

ATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCG

GCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGC

ACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATG AAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAA

CGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATT

AATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCT

TCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGC

GGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCT

ACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAG

ATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATA

TACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATC

CTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGC

GTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGC

GTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGC

CGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCA

GATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAAC

TCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGC

CAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGAT

AAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAG

CGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCC

ACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGG

AACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAG

TCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAG

GGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGG

CCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGG

ATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGAC

CGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAAC

CGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGCGCGCTCGCTCGCTC

ACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCGGGCGACCTTTGGTCGCCCGGCC

TCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCCATCACTAGGGGT

TCCTTGTAGTTAATGATTAACCCGCCATGCTACTTATCTACGTAGCCATGCTCTAG

ATGTCATGGAGAAGACCCACCTTGCAGATGTCCTCACTGGGGCTGGCAGAGCCG

GCAACCTGCCTAAGGCTGCTCAGTCCATTAGGAGCCAGTAGCCTGGAAGATGTCT

TTACCCCCAGCATCAGTTCAAGTGGAGCAGCACATAACTCTTGCCCTCTGCCTTC

CAAGATTCTGGTGCTGAGACTTATGGAGTGTCTTGGAGGTTGCCTTCTGCCCCCC

AACCCTGCTCCCAGCTGGCCCTCCCAGGCCTGGGTTGCTGGCCTCTGCTTTATCA

GGATTCTCAAGAGGGACAGCTGGTTTATGTTGCATGACTGTTCCCTGCATATCTG CTCTGGTTTTAAATAGCTTATCTGAGCAGCTGGAGGACCACATGGGCTTATATGG

CGTGGGGTACATGTTCCTGTAGCCTTGTCCCTGGCACCTGCCAAAATAGCAGCCA

ACACCCCCCACCCCCACCGCCATCCCCCTGCCCCACCCGTCCCCTGTCGCACATT

CCTCCCTCCGCAGGGCTGGCTCACCAGGCCCCAGCCCACATGCCTGCTTAAAGCC

CTCTCCATCCTCTGCCTCACCCAGTCCCCGCTGAGACTGAGCAGACGCCTCCAGA

GCTCGGATCCTGAGA AC TT C AGGGT GAGT C T AT GGGAC

[0122] SEQ ID NO: 11 below is an AAV vector encoding a second portion of MHY7 and including a recombinogenic exogenous sequence:

GGCCGCTTCCCTTTAGTGAGGGTTAATGCTTCGAGCAGACATGATAAGATACATT

GAT GAGTTT GGAC AA ACC AC AACT AGAAT GC AGTGAAA AAAATGCTTT ATTTGT

GAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAG

TTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGA

GGTTTTTTAAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCCGATAAGGGACT

AGAGCATGGCTACGTAGATAAGTAGCATGGCGGGTTAATCATTAACTACAAGGA

ACCCCTAGTGATGGAGTTGGCCACTCCCTCTCTGCGCGCTCGCTCGCTCACTGAG

GCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGGGCGGCCTCAGTG

AGCGAGCGAGCGCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCC

TTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGAATTCCAGACGATTGAGCGT

CAAAATGTAGGTATTTCCATGAGCGTTTTTCCGTTGCAATGGCTGGCGGTAATAT

TGTTCTGGATATTACCAGCAAGGCCGATAGTTTGAGTTCTTCTACTCAGGCAAGT

GAT GTT ATT ACT A AT C A A AGA AGT ATTGC GAC A AC GGTT A ATTT GCGT GAT GGAC

AGACTCTTTTACTCGGTGGCCTCACTGATTATAAAAACACTTCTCAGGATTCTGG

CGTACCGTTCCTGTCTAAAATCCCTTTAATCGGCCTCCTGTTTAGCTCCCGCTCTG

ATTCTAACGAGGAAAGCACGTTATACGTGCTCGTCAAAGCAACCATAGTACGCG

CCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACC

GCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCT

CGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGG

TTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATG

GTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGA

GTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCT

ATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTT

AAAAAAT GAGCTGATTT AAC AAAAATTT AACGCGAATTTT AAC A AAAT ATT AAC

GTCTACAATTTAAATATTTGCTTATACAATCTTCCTGTTTTTGGGGCTTTTCTGATT

ATCAACCGGGGTACATATGATTGACATGCTAGTTTTACGATTACCGTTCATCGAT

TCTCTTGTTTGCTCCAGACTCTCAGGCAATGACCTGATAGCCTTTGTAGAGACCTC

TCAAAAATAGCTACCCTCTCCGGCATGAATTTATCAGCTAGAACGGTTGAATATC

ATATTGATGGTGATTTGACTGTCTCCGGCCTTTCTCACCCGTTTGAATCTTTACCT

ACACATTACTCAGGCATTGCATTTAAAATATATGAGGGTTCTAAAAATTTTTATC

CTTGCGTTGAAATAAAGGCTTCTCCCGCAAAAGTATTACAGGGTCATAATGTTTT

TGGTACAACCGATTTAGCTTTATGCTCTGAGGCTTTATTGCTTAATTTTGCTAATT CTTTGCCTTGCCTGTATGATTTATTGGATGTTGGAATCGCCTGATGCGGTATTTTC

TCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAAT

CTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGAC

GCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCG

TCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAG

ACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATG

GTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTT

GTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGA

T A A AT GC TT C A AT A AT ATT GA A A A AGGA AGAGT AT GAGT ATT C A AC ATTTC CGT G

TCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAA

CGCTGGT GAAAGT AAAAGAT GCTGAAGAT C AGTTGGGTGC ACGAGT GGGTT AC A

TCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAAC

GTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGT

ATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGAC

TTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTA

AGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTA

CTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATG

GGGGAT CAT GT A ACTCGCC TT GAT C GTT GGGA AC CGGAGC T GA AT GA AGC CAT A

CCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGC

AAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACT

GGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTG

GCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCAT

TGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACG

GGG AGT C AGGC A AC TAT GG AT G A AC G A A AT AG AC AG AT C GC T GAG AT AGGT GC C

TCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGA

TTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGAT

AATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACC

CCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTG

CTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAA

GAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAA

ATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGC

ACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGC

GATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCG

CAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACG ACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTT

CCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGG

AGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGT

CGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGG

CGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTT

GCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAAC

CGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGC

GCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCT

CTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGCGCGCTCGCTCGCTCACTGA

GGCCGCCCGGGCAAAGCCCGGGCGTCGGGCGACCTTTGGTCGCCCGGCCTCAGT

GAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCCATCACTAGGGGTTCCTT

GTAGTTAATGATTAACCCGCCATGCTACTTATCTACGTAGCCATGCTCTAGAGTG

ATCCTAGGTGGAGGCCGAAAGTACATGTTTCGCATGGGAACCCCAGACCCTGAG

T ACCC AGATGACT AC AGCC AAGGT GGGACC AGGCTGGACGGGAAGAATCTGGT G

CAGGAATGGCTGGCGAAGCGCCAGGGTGCCCGGTATGTGTGGAACCGCACTGAG

CTCATGCAGGCTTCCCTGGACCCGTCTGTGACCCATCTCATGGGTCTCTTTGAGCC

TGGAGACATGAAATACGAGATCCACCGAGACTCCACACTGGACCCCTCCCTACC

CCTCCCTAGTCATGGCCAACACACACCTTGCCTGCAGGTGTTCTTCAAGGCCGGG

CTGCTGGGGCTGCTGGAGGAAATGAGGGACGAGAGGCTGAGCCGCATCATCACG

CGTATCCAGGCCCAGTCCCGAGGTGTGCTCGCCAGAATGGAGTACAAAAAGCTG

CTGGAACGTAGAGATTCCTTGCTTGTGATTCAGTGGAATATACGAGCTTTCATGG

GAGTAAAAAACTGGCCTTGGATGAAACTCTACTTTAAGATAAAGCCACTGCTGA

AGTCCGCAGAGAGGGAGAAGGAGATGGCATCAATGAAGGAGGAATTTACGCGC

TTGAAAGAGGCTCTCGAGAAGTCCGAAGCTAGACGGAAGGAATTGGAAGAGAA

GATGGTATCTCTTTTGCAGGAGAAGAATGATCTTCAGTTGCAAGTACAAGCGGAA

C AAGAC AATCTCGCCGACGC AGAGGAA AGGTGT GAT C AGTTGATC A AAAAT AAA

ATCCAACTGGAAGCCAAGGTGAAAGAAATGAATGAGCGCTTGGAGGACGAAGA

AG AG AT GA AT GC AGAGTT GACTGCGA AGA AGCGGA AGC T C GA AGAC GA AT GCA

GCGAGCTCAAGCGCGACATTGACGACCTGGAATTGACCCTTGCCAAAGTGGAAA

AGG AG A A AC AT GC A AC C GAG A AT AAGGT A A AG A AT C T G AC AG A AG AG AT GGC G

GGACTTGACGAGATTATCGCGAAGCTTACAAAGGAGAAGAAGGCCCTTCAGGAA

GCGCACCAACAGGCCCTTGACGATCTTCAGGCCGAGG AAGAC AAAGTGAACACA

TTGACTAAAGCTAAAGTGAAGCTTGAGCAACAGGTCGATGACCTGGAAGGCTCA

CTTGAGCAAGAGAAGAAAGTGCGCATGGATCTCGAGCGCGCTAAACGCAAGCTC GAGGGAGATTTGAAGCTGACTCAAGAGTCAATTATGGACCTTGAAAACGACAAG

C AAC AGTT GGAT GAGAGACTT A AGAAGAAGGACTTCGAACTC AACGC ACTT AAT

GCCAGGATCGAAGATGAACAAGCTCTCGGGTCTCAGCTCCAAAAGAAGCTTAAG

GAACTCCAGGCACGCATAGAAGAACTCGAAGAAGAGTTGGAAGCCGAGAGAAC

GGCGAGAGCTAAAGTGGAAAAACTCCGAAGCGATTTGTCCAGAGAGCTGGAAG

AGATTTCCGAGCGGCTTGAGGAGGCGGGAGGCGCGACGAGCGTGCAGATCGAA

ATGAATAAGAAGAGAGAAGCTGAATTTCAAAAAATGCGCCGAGACCTTGAAGA

AGCAACACTCCAGCACGAAGCCACAGCAGCTGCGCTTCGGAAGAAGCATGCTGA

TTCTGTCGC AGAGCTGGGGGAGC AGATCGAC AATCTCC AGCGAGT C AAAC AAAA

ACTGGAAAAGGAGAAATCTGAGTTTAAATTGGAGCTTGACGACGTCACGTCTAA

CAT GGAGC AGAT AAT AAAGGC A AAAGC AAACCTT GAGAAAAT GT GCCGC ACTCT

TGAGGACCAGATGAACGAGCATCGCAGTAAGGCGGAAGAGACTCAGAGATCTGT

TAACGACTTGACCAGTCAACGAGCTAAACTTCAGACAGAAAATGGAGAACTCTC

C C G AC A AC T GG AC G A A A AGG AGGC C C T GAT C AGC C AGT T G AC T AGGGGT AAAC T

CACCTACACACAGCAGCTTGAGGACTTGAAGCGCCAACTCGAAGAGGAGGTGAA

AGCTAAAAACGCACTGGCACATGCGCTGCAATCCGCAAGACATGACTGCGACCT

C C T GC GGG A AC AGT AC G A AG A AG A A AC T G AGGC G A A AGC T G AGC T C C A AC GGG

TACTTTCTAAGGCAAATTCTGAAGTTGCACAGTGGCGAACTAAGTACGAAACCG

ATGCGATCCAACGCACGGAGGAGTTGGAGGAAGCTAAAAAGAAGCTCGCACAG

CGACTGC AGG AAGCCGAAGAAGCCGTTGAGGC AGTT AATGCAAAATGCAGT AGT

CTCGAGAAGACTAAACATAGACTCCAGAACGAAATAGAGGATCTGATGGTGGAT

GTTGAACGCTCCAATGCTGCTGCGGCAGCCCTCGACAAAAAACAGCGGAATTTC

GAT AAGATTCTTGCGGAAT GGAAGC AGAAAT ACGAAGAGTCTC AGAGT GAACTG

GAGAGCTCCCAAAAGGAGGCCCGAAGTCTGTCTACCGAATTGTTTAAACTCAAG

AACGCGTACGAAGAATCTCTGGAACACTTGGAAACCTTTAAAAGAGAGAACAAA

AACCTGCAAGAGGAAATAAGTGACCTTACCGAGCAACTGGGGAGCTCCGGGAAA

ACCATCCACGAATTGGAAAAAGTCAGGAAACAGTTGGAGGCAGAGAAAATGGA

GCTCCAATCCGCCCTGGAGGAAGCGGAAGCATCTCTGGAACACGAGGAGGGCAA

GATTCTGAGGGCCCAACTGGAATTTAACCAGATCAAGGCAGAGATAGAACGAAA

AC T C GC GG AG A AGG AC G A AG AG AT GGAGC AGGC G A A AC GG A AT C ATC T GC GGG

TTGTAGACTCCCTGCAAACCTCCCTCGATGCCGAAACCAGAAGCCGGAATGAGG

CCCTTCGGGTGAAAAAAAAAATGGAGGGCGACTTGAACGAAATGGAAATTCAAC

TTTCTCACGCCAACCGCATGGCGGCCGAGGCGCAGAAACAGGTAAAATCTCTCC

AGTCTCTCCTCAAAGATACACAAATCCAACTCGACGATGCTGTAAGGGCAAACG ATGATTTGAAAGAGAATATAGCAATCGTCGAGCGCCGCAATAATCTGTTGCAAG CAGAGCTTGAAGAACTGCGCGCGGTCGTAGAACAGACCGAACGCAGTAGAAAGT TGGCTGAGCAGGAACTTATTGAGACTTCCGAGCGCGTTCAGCTTCTGCATTCCCA GAAC ACCTCTCTGATT AAT C AGAAGAA AAAGATGGACGC AGATCTGTCTC AGTT GC AGACGGAAGT GGAAGAAGCTGTT C AAGAGT GCCGGAACGCGGAGGAAA AAG C T A A A A AGGC GAT A AC T GAT GC AGC GAT GAT GGC T G AGG A AC T G A AG A AGG AG CAAGACACCTCCGCCCATTTGGAGCGAATGAAGAAGAATATGGAACAAACTATT AAGGATCTCC AGC ACCGGCTT GAT GAGGCTGAAC AGATCGCCTT GAAAGGGGGG AAAAAGCAATTGCAGAAATTGGAAGCCCGAGTAAGGGAACTGGAGAATGAGTT GG A AGC T G AGC A A A AGC GG A AC GC T G AGT C T GT G A AGGG A AT G AG A A AG AGT G A AC GGAGGAT A A A AG A AC T C AC AT AT C A A ACGGA AG AGGACC GGA A A A AC TTG CTCCGCCTCCAAGACCTCGTTGACAAGCTTCAATTGAAAGTCAAGGCTTATAAAA GACAAGCTGAAGAAGCTGAAGAACAAGCGAACACCAATTTGTCCAAATTTCGGA AAGTACAGCATGAGCTCGACGAGGCTGAGGAGCGGGCTGACATAGCTGAGTCCC AGGT C AAT AAACTGCGAGCGAAAAGC AGAGAC ATT GGC ACC AAAGGTTTGAAT G AAGAGGAGGGC AGAGGAAGTCTTCT AAC AT GCGGT GACGT GGAGGAGAATCCC GGCCCT ATGA AAACCTT C AAC ATCTCTC AGC AGGATCTGGAGCTGGT GGAGGT C GCCACTGAGAAGATCACCATGCTCTATGAGGACAACAAGCACCATGTCGGGGCG GCCATCAGGACCAAGACTGGGGAGATCATCTCTGCTGTCCACATTGAAGCCTAC ATTGGCAGGGTCACTGTCTGTGCTGAAGCCATTGCCATTGGGTCTGCTGTGAGCA ACGGGCAGAAGGACTTTGACACCATTGTGGCTGTCAGGCACCCCTACTCTGATGA GGT GGAC AG AT C CAT C AGGGT GGT C AGCC CC T GT GGC AT GT GT AGAGAGCTGAT CTCTGACTATGCTCCTGACTGCTTTGTGCTCATTGAGATGAATGGCAAGCTGGTC AAAACCACCATTGAGGAACTCATCCCCCTCAAGTACACCAGGAACTAATAAGC