Title:
GENE TRANSCRIPTION FRAMEWORK, VECTOR SYSTEM, GENOME SEQUENCE EDITING METHOD AND APPLICATION
Document Type and Number:
WIPO Patent Application WO/2022/156378
Kind Code:
A1
Abstract:
Provided is a gene transcription framework, a vector system, and a genome sequence editing method and application. The gene transcription framework is based on an eukaryotic retrotransposition mechanism and may be mediated by DNA, RNA, or RNP pathways, and comprised are a transcript sequence comprising a target site upstream sequence, a sequence to be inserted, and a target site downstream sequence, one or more Sine (elements), a LINE (element), one or more ORF1p coding sequences, and/or an ORF2p coding sequence. In the gene editing method, insofar as foreign systems or substances are as much as possible not introduced and no double-stranded breaks are generated, transfer into the nucleus or cytoplasm occurs by means of the DNA, RNA or RNP pathways (RNA or RNP is mediated to be transferred from the cytoplasm to the cell nucleus by means of the ORF1p and/or the ORF2p), and a target fragment is inserted into a specified site in the genome or a specified fragment in the genome is deleted or replaced.
Inventors:
PENG SHUANGHONG (CN)
SUI YUNPENG (CN)
SUI YUNPENG (CN)
Application Number:
PCT/CN2021/134710
Publication Date:
July 28, 2022
Filing Date:
December 01, 2021
Export Citation:
Assignee:
PENG SHUANGHONG (CN)
International Classes:
C12N15/85; A61K48/00; A61P25/16; A61P25/28; A61P35/00; A61P35/02; A61P43/00; C12N15/63
Domestic Patent References:
| WO2020047124A1 | 2020-03-05 | |||
| WO2020082076A1 | 2020-04-23 |
Foreign References:
| CN102080101A | 2011-06-01 |
Other References:
SUI YUNPENG, PENG SHUANGHONG: "A Mechanism Leading to Changes in Copy Number Variations Affected by Transcriptional Level Might Be Involved in Evolution, Embryonic Development, Senescence, and Oncogenesis Mediated by Retrotransposons", FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY, vol. 9, 11 February 2021 (2021-02-11), pages 1 - 27, XP055952845, DOI: 10.3389/fcell.2021.618113
ZHENG FEIYANG, KAWABE YOSHINORI, MURAKAMI MAI, TAKAHASHI MAMIKA, YOSHIDA SHOICHIRO, ITO AKIRA, KAMIHIRA MASAMICHI: "Retrotransposon-mediated Gene Transfer for Animal Cells", MATEC WEB OF CONFERENCES, vol. 333, 1 January 2021 (2021-01-01), pages 1 - 5, XP055952848, DOI: 10.1051/matecconf/202133307002
LI HONGYU;ZHENG XIN;LONG MIN;ZHANG TIAN;ZHANG JICHUN;LI JING: "The Impact of Retroposons on Primate Genome Structure", SICHUAN JOURNAL OF ZOOLOGY, vol. 34, no. 2, 28 March 2015 (2015-03-28), pages 315 - 320, XP055952850, ISSN: 1000-7083, DOI: 10.3969/j.issn.1000-7083.2015.02.025
ZHU QIXIA, LI YIXIANG;CHENG CHUNYAN;HU WENJIN;ZHOU LIQIN: "The Regulation of Retrotransposon LINE-1 and Its Function in Tumorigenesis", GUANGXI SCIENCES, vol. 25, no. 3, 15 June 2018 (2018-06-15), pages 268 - 273, XP055952852, DOI: 10.13656/j.cnki.gxkx.20180604.002
ZHENG FEIYANG, KAWABE YOSHINORI, MURAKAMI MAI, TAKAHASHI MAMIKA, YOSHIDA SHOICHIRO, ITO AKIRA, KAMIHIRA MASAMICHI: "Retrotransposon-mediated Gene Transfer for Animal Cells", MATEC WEB OF CONFERENCES, vol. 333, 1 January 2021 (2021-01-01), pages 1 - 5, XP055952848, DOI: 10.1051/matecconf/202133307002
LI HONGYU;ZHENG XIN;LONG MIN;ZHANG TIAN;ZHANG JICHUN;LI JING: "The Impact of Retroposons on Primate Genome Structure", SICHUAN JOURNAL OF ZOOLOGY, vol. 34, no. 2, 28 March 2015 (2015-03-28), pages 315 - 320, XP055952850, ISSN: 1000-7083, DOI: 10.3969/j.issn.1000-7083.2015.02.025
ZHU QIXIA, LI YIXIANG;CHENG CHUNYAN;HU WENJIN;ZHOU LIQIN: "The Regulation of Retrotransposon LINE-1 and Its Function in Tumorigenesis", GUANGXI SCIENCES, vol. 25, no. 3, 15 June 2018 (2018-06-15), pages 268 - 273, XP055952852, DOI: 10.13656/j.cnki.gxkx.20180604.002
Attorney, Agent or Firm:
BEIJING BEIXIN-ZHICHENG INTELLECTUAL PROPERTY AGENT CO., LTD. (CN)
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