Title:
GENETICALLY ENGINEERED BACTERIUM FOR CO-PRODUCTION OF 3-HYDROXYPROPIONIC ACID AND 1,3-PROPANEDIOL, AND CONSTRUCTION METHOD THEREFOR AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/159745
Kind Code:
A1
Abstract:
The present application relates to the technical field of bioengineering, and provides a genetically engineered bacterium for co-production of 3-hydroxypropionic acid and 1,3-propanediol, and a construction method therefor and an application thereof. Escherichia coli W3110 (DE3) is used as a starting strain, a genetically engineered bacterium for co-production of 3-hydroxypropionic acid and 1,3-propanediol is constructed by utilizing the technical means of genetic engineering, and the fermentation process of the constructed genetically engineered bacterium is optimized, such that efficient co-production of 3-hydroxypropionic acid and 1,3-propanediol is realized.
Inventors:
QI XIANGHUI (CN)
ZHANG YUFEI (CN)
YUAN JUNHUA (CN)
DOU YUAN (CN)
ZHAO MEI (CN)
ZHAI BIDE (CN)
ZHANG YUFEI (CN)
YUAN JUNHUA (CN)
DOU YUAN (CN)
ZHAO MEI (CN)
ZHAI BIDE (CN)
Application Number:
PCT/CN2022/089426
Publication Date:
August 31, 2023
Filing Date:
April 27, 2022
Export Citation:
Assignee:
UNIV JIANGSU (CN)
International Classes:
C12P7/18; C12N1/21; C12P7/42; C12R1/19
Foreign References:
| CN112210524A | 2021-01-12 | |||
| CN112625994A | 2021-04-09 | |||
| CN105647845A | 2016-06-08 | |||
| CN112226397A | 2021-01-15 | |||
| CN105567622A | 2016-05-11 | |||
| US20110117617A1 | 2011-05-19 |
Other References:
HUN SU CHU; YOUNG SOO KIM; CHAN MU LEE; JU HEE LEE; WON SEOK JUNG; JIN‐HO AHN; SEUNG HOON SONG; IN SUK CHOI; KWANG MYUNG CHO: "Metabolic engineering of 3‐hydroxypropionic acid biosynthesis in Escherichia coli", BIOTECHNOLOGY AND BIOENGINEERING, JOHN WILEY, HOBOKEN, USA, vol. 112, no. 2, 13 October 2014 (2014-10-13), Hoboken, USA, pages 356 - 364, XP071155356, ISSN: 0006-3592, DOI: 10.1002/bit.25444
CHEN ZHEN, LIU DEHUA: "Toward glycerol biorefinery: metabolic engineering for the production of biofuels and chemicals from glycerol", BIOTECHNOLOGY FOR BIOFUELS, vol. 9, no. 1, 1 December 2016 (2016-12-01), pages 205, XP093086989, DOI: 10.1186/s13068-016-0625-8
VIDRA ALADÁR, NÉMETH ÁRON: "Bio-based 3-hydroxypropionic Acid: A Review", PERIODICA POLYTECHNICA. CHEMICAL ENGINEERING, BUDAPEST, HU, vol. 62, no. 2, 21 September 2017 (2017-09-21), HU , pages 156, XP093086991, ISSN: 0324-5853, DOI: 10.3311/PPch.10861
ZHANG YUFEI, ZABED HOSSAIN M., YUN JUNHUA, ZHANG GUOYAN, WANG YANG, QI XIANGHUI: "Notable Improvement of 3-Hydroxypropionic Acid and 1,3-Propanediol Coproduction Using Modular Coculture Engineering and Pathway Rebalancing", ACS SUSTAINABLE CHEMISTRY & ENGINEERING, AMERICAN CHEMICAL SOCIETY, US, vol. 9, no. 12, 29 March 2021 (2021-03-29), US , pages 4625 - 4637, XP093086993, ISSN: 2168-0485, DOI: 10.1021/acssuschemeng.1c00229
MIAOMIAO YANG; JUNHUA YUN; HUANHUAN ZHANG; GUOYAN ZHANG; HOSSAIN ZABED; XIANGHUI QI: "Bottlenecks and Modification Strategies of 1,3-propanediol Biosynthesis from Glycerol", CHINESE JOURNAL OF BIOTECHNOLOGY, ZHONGGUO KEXUEYUAN WEISHENGWU YANJIUSUO, CHINESE ACADEMY OF SCIENCES, INSTITUTE OF MICROBIOLOGY, CN, vol. 34, no. 7, 25 July 2018 (2018-07-25), CN , pages 1069 - 1080, XP009548291, ISSN: 1000-3061, DOI: 10.13345/j.cjb.170516
CHEN ZHEN, LIU DEHUA: "Toward glycerol biorefinery: metabolic engineering for the production of biofuels and chemicals from glycerol", BIOTECHNOLOGY FOR BIOFUELS, vol. 9, no. 1, 1 December 2016 (2016-12-01), pages 205, XP093086989, DOI: 10.1186/s13068-016-0625-8
VIDRA ALADÁR, NÉMETH ÁRON: "Bio-based 3-hydroxypropionic Acid: A Review", PERIODICA POLYTECHNICA. CHEMICAL ENGINEERING, BUDAPEST, HU, vol. 62, no. 2, 21 September 2017 (2017-09-21), HU , pages 156, XP093086991, ISSN: 0324-5853, DOI: 10.3311/PPch.10861
ZHANG YUFEI, ZABED HOSSAIN M., YUN JUNHUA, ZHANG GUOYAN, WANG YANG, QI XIANGHUI: "Notable Improvement of 3-Hydroxypropionic Acid and 1,3-Propanediol Coproduction Using Modular Coculture Engineering and Pathway Rebalancing", ACS SUSTAINABLE CHEMISTRY & ENGINEERING, AMERICAN CHEMICAL SOCIETY, US, vol. 9, no. 12, 29 March 2021 (2021-03-29), US , pages 4625 - 4637, XP093086993, ISSN: 2168-0485, DOI: 10.1021/acssuschemeng.1c00229
MIAOMIAO YANG; JUNHUA YUN; HUANHUAN ZHANG; GUOYAN ZHANG; HOSSAIN ZABED; XIANGHUI QI: "Bottlenecks and Modification Strategies of 1,3-propanediol Biosynthesis from Glycerol", CHINESE JOURNAL OF BIOTECHNOLOGY, ZHONGGUO KEXUEYUAN WEISHENGWU YANJIUSUO, CHINESE ACADEMY OF SCIENCES, INSTITUTE OF MICROBIOLOGY, CN, vol. 34, no. 7, 25 July 2018 (2018-07-25), CN , pages 1069 - 1080, XP009548291, ISSN: 1000-3061, DOI: 10.13345/j.cjb.170516
Attorney, Agent or Firm:
NANJING DOYES INTELLECTUAL PROPERTY CO., LTD. (CN)
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