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Title:
A GENETICALLY ENGINEERED BACTERIUM AND A PREPARATION METHOD AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/103578
Kind Code:
A1
Abstract:
The present invention discloses a genetically engineered bacterium and a preparation method and use thereof. The genetically engineered bacteria contain a gene encoding α-1, 2-fucosyltransferase, and a gene encoding a protein tag is connected to the gene encoding α-1, 2-fucosyltransferase; the protein tag is MBP, SUMO1, SUMO2 or TrxA, the amino acid sequence of the MBP is shown in SEQ ID NO: 2, the amino acid sequence of the SUMO1 is shown in SEQ ID NO: 3, the amino acid sequence of the SUMO2 is shown in SEQ ID NO: 4, the amino acid sequence of the TrxA is shown in SEQ ID NO: 5. Fermentation with the genetically engineered bacteria can greatly increase the yield of 2'-fucosyllactose compared to the genetically engineered bacteria that only expresses α-1, 2-fucosyltransferase exogenously, and the yield can be more than doubled in a preferred case.

Inventors:
WU YAN (CN)
LIU YUROU (CN)
TANG JING (CN)
XU HONG (CN)
ZHAO JIN (CN)
Application Number:
PCT/CN2022/124826
Publication Date:
June 15, 2023
Filing Date:
October 12, 2022
Export Citation:
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Assignee:
SYNAURA BIOTECHNOLOGY SHANGHAI CO LTD (CN)
International Classes:
C12P19/18; C12N9/10
Foreign References:
CN112322565A2021-02-05
CN111808790A2020-10-23
US20170152538A12017-06-01
EP3425052A12019-01-09
CN112322565A2021-02-05
Other References:
LIN LU ET AL: "Combinatorial metabolic engineering of Escherichia coli for de novo production of 2'-fucosyllactose", BIORESOURCE TECHNOLOGY, ELSEVIER, AMSTERDAM, NL, vol. 351, 4 March 2022 (2022-03-04), XP087014275, ISSN: 0960-8524, [retrieved on 20220304], DOI: 10.1016/J.BIORTECH.2022.126949
Attorney, Agent or Firm:
LIU, SHEN & ASSOCIATES (CN)
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