Title:
A GENETICALLY ENGINEERED BACTERIUM WITH LACZ INACTIVATION AND ITS USE IN PRODUCING HUMAN MILK OLIGOSACCHARIDES
Document Type and Number:
WIPO Patent Application WO/2023/093337
Kind Code:
A1
Abstract:
The present invention discloses a genetically engineered bacteria, which is E. coli integrated with lysogenic λDE3, and lacZ gene is completely inactivated, but does not affect exogenous protein expression of the genetically engineered bacteria. The present invention also discloses a method for culturing the genetically engineered bacteria, and a method for preparing human milk oligosaccharides using the same, and use of the genetically engineered bacteria. The genetically engineered bacteria of the present invention can efficiently produce human milk oligosaccharides, such as 2'-fucosyllactose, and have wide industrial application prospects.
Inventors:
JIAO QI (CN)
LIU YUROU (CN)
TIAN ZHENHUA (CN)
XU HONG (CN)
WANG SHU (CN)
LIU YUROU (CN)
TIAN ZHENHUA (CN)
XU HONG (CN)
WANG SHU (CN)
Application Number:
PCT/CN2022/124632
Publication Date:
June 01, 2023
Filing Date:
October 11, 2022
Export Citation:
Assignee:
SYNAURA BIOTECHNOLOGY SHANGHAI CO LTD (CN)
International Classes:
A23L33/00; C07H1/00; C07H3/06; C12N1/20; C12N9/38; C12P19/12; C12P19/14
Foreign References:
| CN110804577A | 2020-02-18 | |||
| US4963469A | 1990-10-16 | |||
| CN109790559A | 2019-05-21 |
Other References:
ZHANG ANGELA ET AL: "Microbial production of human milk oligosaccharide lactodifucotetraose", METABOLIC ENGINEERING, vol. 66, July 2021 (2021-07-01), AMSTERDAM, NL, pages 12 - 20, XP055914876, ISSN: 1096-7176, DOI: 10.1016/j.ymben.2021.03.014
TEKALIGN DERESSA ET AL: "Structural Integrity of the Antigen Is a Determinant for the Induction of T-Helper Type-1 Immunity in Mice by Gene Gun Vaccines against E.coli Beta-Galactosidase", PLOS ONE, vol. 9, no. 7, 15 July 2014 (2014-07-15), pages e102280, XP055309170, DOI: 10.1371/journal.pone.0102280
JOHN C GEBLER ET AL: "Glu-537, Not Glu-461, Is the Nucleophile in the Active Site of (lacZ) beta-Galactosidase from Escherichia coli", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 267, no. 16, 5 June 1992 (1992-06-05), pages 11126 - 11130, XP055518357, Retrieved from the Internet
JOURNAL OF BIOTECHNOLOGY, vol. 210, 2015, pages 107 - 115
ZHANG ET AL., METABOLIC ENGINEERING, vol. 66, 2021, pages 12 - 2
"GenBank", Database accession no. AAX45030.1
TEKALIGN DERESSA ET AL: "Structural Integrity of the Antigen Is a Determinant for the Induction of T-Helper Type-1 Immunity in Mice by Gene Gun Vaccines against E.coli Beta-Galactosidase", PLOS ONE, vol. 9, no. 7, 15 July 2014 (2014-07-15), pages e102280, XP055309170, DOI: 10.1371/journal.pone.0102280
JOHN C GEBLER ET AL: "Glu-537, Not Glu-461, Is the Nucleophile in the Active Site of (lacZ) beta-Galactosidase from Escherichia coli", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 267, no. 16, 5 June 1992 (1992-06-05), pages 11126 - 11130, XP055518357, Retrieved from the Internet
JOURNAL OF BIOTECHNOLOGY, vol. 210, 2015, pages 107 - 115
ZHANG ET AL., METABOLIC ENGINEERING, vol. 66, 2021, pages 12 - 2
"GenBank", Database accession no. AAX45030.1
Attorney, Agent or Firm:
LIU, SHEN & ASSOCIATES (CN)
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