Title:
GENETICALLY MODIFIED YEAST OF THE GENUS YARROWIA CAPABLE OF PRODUCING VITAMIN A
Document Type and Number:
WIPO Patent Application WO/2023/044937
Kind Code:
A1
Abstract:
The present invention generally relates to the biotechnology engineering, and specifically to genetically modified yeast, which allow for the production of vitamin A. More specifically, the present invention provides a genetically modified yeast of the genus Yarrowia capable of producing vitamin A. The present invention also provides methods for the production of vitamin A using a genetically modified yeast of the invention.
Inventors:
KONTE TILEN (SI)
KAVSCEK MARTIN (SI)
MARKUS TADEJ (SI)
SVAGELJ MIRJAN (SI)
FUJS STEFAN (SI)
SHI MINGAN (CN)
XIA YUNCHONG (CN)
SUN XIANGYU (CN)
ZUO JING (CN)
SUN JIA (CN)
CAI ZHIGANG (CN)
ZHANG GUOYING (CN)
KAVSCEK MARTIN (SI)
MARKUS TADEJ (SI)
SVAGELJ MIRJAN (SI)
FUJS STEFAN (SI)
SHI MINGAN (CN)
XIA YUNCHONG (CN)
SUN XIANGYU (CN)
ZUO JING (CN)
SUN JIA (CN)
CAI ZHIGANG (CN)
ZHANG GUOYING (CN)
Application Number:
PCT/CN2021/121111
Publication Date:
March 30, 2023
Filing Date:
September 27, 2021
Export Citation:
Assignee:
CHIFENG PHARMACEUTICAL CO LTD (CN)
International Classes:
C12P23/00; C12N9/00; C12N9/02; C12N9/04; C12N9/10; C12N9/20; C12N9/90; C12N15/52
Domestic Patent References:
| WO2021009194A1 | 2021-01-21 | |||
| WO2019057999A1 | 2019-03-28 | |||
| WO2008042338A2 | 2008-04-10 | |||
| WO2020111890A1 | 2020-06-04 |
Foreign References:
| US8846374B2 | 2014-09-30 | |||
| US9297031B2 | 2016-03-29 | |||
| CN111107832A | 2020-05-05 | |||
| CN111108194A | 2020-05-05 | |||
| CN111107834A | 2020-05-05 | |||
| US4683195A | 1987-07-28 |
Other References:
SUN L. ET AL: "Vitamin A Production by Engineered Saccharomyces cerevisiae from Xylose via Two-Phase in Situ Extraction", ACS SYNTHETIC BIOLOGY, vol. 8, no. 9, 20 September 2019 (2019-09-20), Washington DC ,USA, pages 2131 - 2140, XP055883753, ISSN: 2161-5063, Retrieved from the Internet DOI: 10.1021/acssynbio.9b00217
LARROUDE M. ET AL: "A synthetic biology approach to transform Yarrowia lipolytica into a competitive biotechnological producer of [beta]-carotene", BIOTECHNOLOGY AND BIOENGINEERING, vol. 115, no. 2, 3 November 2017 (2017-11-03), pages 464 - 472, XP071153744, ISSN: 0006-3592, DOI: 10.1002/BIT.26473
ZWEYTICK, D.LEITNER, E.KOHLWEIN, S.D. ET AL.: "Contribution of Arelp and Are2p to steryl ester synthesis in the yeast Saccharomyces cerevisiae", EUR J BIOCHEM, vol. 267, no. 4, 2000, pages 1075 - 82, XP055059359, DOI: 10.1046/j.1432-1327.2000.01103.x
SAMBROOK ET AL.: "Molecular Cloning: A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
B. PERBAL, A PRACTICAL GUIDE TO MOLECULAR CLONING, 1984
R. I. FRESHNEY: "Culture Of Animal Cells", 1987, ALAN R. LISS, INC.
"Immobilized Cells And Enzymes", 1986, IRL PRESS
SAMBROOK ET AL.: "Molecular cloning: A Laboratory Manual", vol. 154, 155, 1989, COLD SPRING HARBOR LABORATORY
LIANG S.SURYANG K.YONG-SU J: "Vitamin A Production by Engineered Saccharomyces cerevisiae from Xylose via Two-Phase in Situ Extraction", ACS SYNTHETIC BIOLOGY, vol. 8, no. 9, 2019, pages 2131 - 2140
ZHANG, X.-K.WANG, D.-N.CHEN, J ET AL.: "Metabolic engineering of β-carotene biosynthesis in Yarrowia lipolytica", BIOTECHNOL LETT, vol. 42, 2020, pages 945 - 956
LARROUDE, M.CELINSKA, E.BACK, A. ET AL.: "A synthetic biology approach to transform Yarrowia lipolytica into a competitive biotechnological producer of β-carotene", BIOTECHNOLOGY AND BIOENGINEERING, vol. 115, no. 2, 2018, pages 464 - 472, XP071153744, DOI: 10.1002/bit.26473
CHOI, B.H.HWANG, H.J.LEE, J.E. ET AL.: "Microbial Production of Retinyl Palmitate and Its Application as a Cosmeceutical", ANTIOXIDANTS, vol. 9, 2020, pages 1130
BARJA, M. V.RODRIGUEZ-CONCEPCION, M. A: "Simple In Vitro Assay to Measure the Activity of Geranylgeranyl Diphosphate Synthase and Other Short-Chain Prenyltransferases", METHODS MOL BIOL, vol. 2083, 2020, pages 27 - 38
KOSCHMIEDER, J., FEHLING-KASCHEK, M., SCHAUB, P: "Plant-type phytoene desaturase: Functional evaluation of structural implications", PLOS ONE, vol. 12, no. 11, 2017, pages e0187628
BREITENBACH, J.FRASER, P.D.SANDMANN, G: "Carotenoid synthesis and phytoene synthase activity during mating of Blakeslea trispora", PHYTOCHEMISTRY, vol. 76, 2012, pages 40 - 45
YU, Q.SCHAUB, P.GHISLA, S. ET AL.: "The lycopene cyclase CrtY from Pantoea ananatis (formerly Erwinia uredovora) catalyzes an FADred-dependent non-redox reaction", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 285, no. 16, 2010, pages 12109 - 12120
KIM, Y.-S.KIM, N.-H.YEOM, S.-J. ET AL.: "In Vitro Characterization of a Recombinant Blh Protein from an Uncultured Marine Bacterium as a β-Carotene 15,15'-Dioxygenase", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 284, no. 23, 2009, pages 15781 - 15793
BHUTADA, G.KAVSCEK, M.LEDESMA-AMARO, R. ET AL.: "Sugar versus fat: elimination of glycogen storage improves lipid accumulation in Yarrowia lipolytica", FEMS YEAST RES., vol. 17, no. 3, 2017, pages fox020
KNOLL, L. J.JOHNSON, D. R.GORDON, J. I.: "Biochemical studies of three Saccharomyces cerevisiae acyl-CoA synthetases, Faa1 p, Faa2p, and Faa3p", J BIOL CHEM., vol. 269, no. 23, 1994, pages 16348 - 16356, XP002904759
HONG, L.BLACK, P. N.CHOKSHI, A. ET AL.: "High-throughput screening for fatty acid uptake inhibitors in humanized yeast identifies atypical antipsychotic drugs that cause dyslipidemias", JOURNAL OF LIPID RESEARCH, vol. 49, no. 1, 2008, pages 230 - 244
LARROUDE M. ET AL: "A synthetic biology approach to transform Yarrowia lipolytica into a competitive biotechnological producer of [beta]-carotene", BIOTECHNOLOGY AND BIOENGINEERING, vol. 115, no. 2, 3 November 2017 (2017-11-03), pages 464 - 472, XP071153744, ISSN: 0006-3592, DOI: 10.1002/BIT.26473
ZWEYTICK, D.LEITNER, E.KOHLWEIN, S.D. ET AL.: "Contribution of Arelp and Are2p to steryl ester synthesis in the yeast Saccharomyces cerevisiae", EUR J BIOCHEM, vol. 267, no. 4, 2000, pages 1075 - 82, XP055059359, DOI: 10.1046/j.1432-1327.2000.01103.x
SAMBROOK ET AL.: "Molecular Cloning: A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
B. PERBAL, A PRACTICAL GUIDE TO MOLECULAR CLONING, 1984
R. I. FRESHNEY: "Culture Of Animal Cells", 1987, ALAN R. LISS, INC.
"Immobilized Cells And Enzymes", 1986, IRL PRESS
SAMBROOK ET AL.: "Molecular cloning: A Laboratory Manual", vol. 154, 155, 1989, COLD SPRING HARBOR LABORATORY
LIANG S.SURYANG K.YONG-SU J: "Vitamin A Production by Engineered Saccharomyces cerevisiae from Xylose via Two-Phase in Situ Extraction", ACS SYNTHETIC BIOLOGY, vol. 8, no. 9, 2019, pages 2131 - 2140
ZHANG, X.-K.WANG, D.-N.CHEN, J ET AL.: "Metabolic engineering of β-carotene biosynthesis in Yarrowia lipolytica", BIOTECHNOL LETT, vol. 42, 2020, pages 945 - 956
LARROUDE, M.CELINSKA, E.BACK, A. ET AL.: "A synthetic biology approach to transform Yarrowia lipolytica into a competitive biotechnological producer of β-carotene", BIOTECHNOLOGY AND BIOENGINEERING, vol. 115, no. 2, 2018, pages 464 - 472, XP071153744, DOI: 10.1002/bit.26473
CHOI, B.H.HWANG, H.J.LEE, J.E. ET AL.: "Microbial Production of Retinyl Palmitate and Its Application as a Cosmeceutical", ANTIOXIDANTS, vol. 9, 2020, pages 1130
BARJA, M. V.RODRIGUEZ-CONCEPCION, M. A: "Simple In Vitro Assay to Measure the Activity of Geranylgeranyl Diphosphate Synthase and Other Short-Chain Prenyltransferases", METHODS MOL BIOL, vol. 2083, 2020, pages 27 - 38
KOSCHMIEDER, J., FEHLING-KASCHEK, M., SCHAUB, P: "Plant-type phytoene desaturase: Functional evaluation of structural implications", PLOS ONE, vol. 12, no. 11, 2017, pages e0187628
BREITENBACH, J.FRASER, P.D.SANDMANN, G: "Carotenoid synthesis and phytoene synthase activity during mating of Blakeslea trispora", PHYTOCHEMISTRY, vol. 76, 2012, pages 40 - 45
YU, Q.SCHAUB, P.GHISLA, S. ET AL.: "The lycopene cyclase CrtY from Pantoea ananatis (formerly Erwinia uredovora) catalyzes an FADred-dependent non-redox reaction", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 285, no. 16, 2010, pages 12109 - 12120
KIM, Y.-S.KIM, N.-H.YEOM, S.-J. ET AL.: "In Vitro Characterization of a Recombinant Blh Protein from an Uncultured Marine Bacterium as a β-Carotene 15,15'-Dioxygenase", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 284, no. 23, 2009, pages 15781 - 15793
BHUTADA, G.KAVSCEK, M.LEDESMA-AMARO, R. ET AL.: "Sugar versus fat: elimination of glycogen storage improves lipid accumulation in Yarrowia lipolytica", FEMS YEAST RES., vol. 17, no. 3, 2017, pages fox020
KNOLL, L. J.JOHNSON, D. R.GORDON, J. I.: "Biochemical studies of three Saccharomyces cerevisiae acyl-CoA synthetases, Faa1 p, Faa2p, and Faa3p", J BIOL CHEM., vol. 269, no. 23, 1994, pages 16348 - 16356, XP002904759
HONG, L.BLACK, P. N.CHOKSHI, A. ET AL.: "High-throughput screening for fatty acid uptake inhibitors in humanized yeast identifies atypical antipsychotic drugs that cause dyslipidemias", JOURNAL OF LIPID RESEARCH, vol. 49, no. 1, 2008, pages 230 - 244
Attorney, Agent or Firm:
KANGXIN PARTNERS, P.C. (CN)
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