Title:
GENOTYPING METHOD OF TYPE OR SUBTYPE OF PATHOGEN SUCH AS VIRUS, BACTERIUM, AND PARASITE
Document Type and Number:
WIPO Patent Application WO/2017/051834
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a primer set for PCR and LAMP to determine the type or subtype of a pathogen such as a virus, bacterium, or parasite, especially the subtype of an influenza virus, a method for determining the type or subtype using the primer set, and a kit for implementing the method. The present invention is a gene (RNA or DNA) that serves as a basis for classification of the type or subtype of a pathogen such as a virus, bacterium, or parasite, wherein all genes that belong to a type or subtype are divided into several small groups on the basis of the similarity of the base sequence, and the primers are designed so that the genes belonging to each small group can be detected by the smallest number of primers possible. The present invention is also a method for determining the type or subtype of a pathogen such as a virus, bacterium, or parasite that is the detection target by using primers prepared as described above.
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Inventors:
TSUKAMOTO KENJI (JP)
MURAKAMI HIRONOBU (JP)
MURAKAMI HIRONOBU (JP)
Application Number:
PCT/JP2016/077917
Publication Date:
March 30, 2017
Filing Date:
September 22, 2016
Export Citation:
Assignee:
SCHOOL CORP AZABU VETERINARY MEDICINE EDUCATIONAL INST (JP)
International Classes:
C12N15/09; C12M1/00; C12M1/34; C12Q1/04; C12Q1/68; C12Q1/70
Foreign References:
| JP2010252659A | 2010-11-11 |
Other References:
TSUKAMOTO K. ET AL.: "SYBR Green-Based Real- Time Reverse Transcription-PCR for Typing and Subtyping of All Hemagglutinin and Neuraminidase Genes of Avian Influenza Virus and Comparison to Standard Serological Subtyping Tests", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 50, no. 1, 26 October 2011 (2011-10-26), pages 37 - 45, XP055372677
QIU B. F. ET AL.: "A reverse transcription-PCR for subtyping of neuraminidase of avian influenza viruses", JOURNAL OF VIROLOGICAL METHODS, vol. 155, no. 2, 2009, pages 193 - 198, XP025837657
KENJI TSUKAMOTO ET AL.: "Jinju Kyotsu Kansensho no Yokusei no Tameno Gijutsu Kaihatsu Dai 4 Hen Jinju Kyotsu Kansensho no Kan'i'Jinsoku Shindan Gijutsu no Kaihatsu (1) Tori Influenza no Jinsoku Shindanho no Kaihatsu", THE MINISTRY OF AGRICULTURE, FORESTRY AND FISHERIES OF JAPAN NORIN SUISAN GIJUTSU KAIGI JIMUKYOKU KENKYU SEIKA, vol. 469, 2009, pages 163 - 169
KENJI TSUKAMOTO ET AL.: "KoByogensei Tori Influenza Ryuko Tori Influenza no Tameno Real Time PCR", JOURNAL OF VETERINARY MEDICINE, vol. 65, no. 11, 2012, pages 907 - 914
YUHEI KOYAMA ET AL.: "Population Primer o Riyo shita, H5 Agata Tori Influenza Virus no Morateki Kenshutsu", DAI 159 KAI JAPANESE SOCIETY OF VETERIANY SCIENCE GAKUJUTSU SHUKAI KOEN YOSHISHU, 30 August 2016 (2016-08-30), pages 372
TSUKAMOTO K. ET AL.: "Broad Detection of Diverse H5 and H7 Hemagglutinin Genes of Avian Influenza Viruses by Real-Time Reverse Transcription-PCR Using Primer and Probe Sets Containing Mixed Bases", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 48, no. 11, November 2010 (2010-11-01), pages 4275 - 4278, XP055372685
QIU B. F. ET AL.: "A reverse transcription-PCR for subtyping of neuraminidase of avian influenza viruses", JOURNAL OF VIROLOGICAL METHODS, vol. 155, no. 2, 2009, pages 193 - 198, XP025837657
KENJI TSUKAMOTO ET AL.: "Jinju Kyotsu Kansensho no Yokusei no Tameno Gijutsu Kaihatsu Dai 4 Hen Jinju Kyotsu Kansensho no Kan'i'Jinsoku Shindan Gijutsu no Kaihatsu (1) Tori Influenza no Jinsoku Shindanho no Kaihatsu", THE MINISTRY OF AGRICULTURE, FORESTRY AND FISHERIES OF JAPAN NORIN SUISAN GIJUTSU KAIGI JIMUKYOKU KENKYU SEIKA, vol. 469, 2009, pages 163 - 169
KENJI TSUKAMOTO ET AL.: "KoByogensei Tori Influenza Ryuko Tori Influenza no Tameno Real Time PCR", JOURNAL OF VETERINARY MEDICINE, vol. 65, no. 11, 2012, pages 907 - 914
YUHEI KOYAMA ET AL.: "Population Primer o Riyo shita, H5 Agata Tori Influenza Virus no Morateki Kenshutsu", DAI 159 KAI JAPANESE SOCIETY OF VETERIANY SCIENCE GAKUJUTSU SHUKAI KOEN YOSHISHU, 30 August 2016 (2016-08-30), pages 372
TSUKAMOTO K. ET AL.: "Broad Detection of Diverse H5 and H7 Hemagglutinin Genes of Avian Influenza Viruses by Real-Time Reverse Transcription-PCR Using Primer and Probe Sets Containing Mixed Bases", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 48, no. 11, November 2010 (2010-11-01), pages 4275 - 4278, XP055372685
Attorney, Agent or Firm:
OKUHARA Koji et al. (JP)
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