Title:
GH117A Α-NEOAGAROBIOSE HYDROLASE DERIVED FROM NOVEL AGAR-DEGRADING BACTERIA, AND L-AHG PRODUCTION METHOD USING SAME
Document Type and Number:
WIPO Patent Application WO/2023/080357
Kind Code:
A1
Abstract:
The present invention relates to a GH117A α-neoagarobiose hydrolase (α-NABH) derived from novel agar-degrading bacteria Cellvibrio sp. KY-GH-1 deposited with an accession number KCTC 13629BP, and a method for producing 3,6-anhydro-L-galactose (L-AHG) by using the enzyme. GH117A α-neoagarobiose hydrolase derived from novel gar-degrading bacteria Cellvibrio sp. KY-GH-1, of the present invention, uses neoagarobiose as a substrate so as to be capable of producing L-AHG with high efficiency without being significantly limited by temperature and pH ranges, and thus has the excellent effect of being widely usable in the fermentation, food, pharmaceutical industries, and the like.
Inventors:
KIM YOUNG HO (KR)
Application Number:
PCT/KR2022/004631
Publication Date:
May 11, 2023
Filing Date:
March 31, 2022
Export Citation:
Assignee:
KYUNGPOOK NAT UNIV IND ACADEMIC COOP FOUND (KR)
International Classes:
C12N9/24; C12N1/20; C12N15/70; C12P19/02; C12R1/01
Foreign References:
| JP2013247884A | 2013-12-12 |
Other References:
JANG, W. Y. ET AL.: "A Novel Neoagarobiose-Specific GH117A α-NABH from a Freshwater Agar-Degrading Bacterium Cellvibrio sp. KY-GH-1", KMB 2020 47TH ANNUAL MEETING & INTERNATIONAL SYMPOSIUM, 25 September 2020 (2020-09-25), pages 470 - 486, XP009546611
KWON GI HYUN, KWON MI JUNG, PARK JU EUN, KIM YOUNG HO: "Whole genome sequence of a freshwater agar-degrading bacterium Cellvibrio sp. KY-GH-1", BIOTECHNOLOGY REPORTS, ELSEVIER, vol. 23, 1 September 2019 (2019-09-01), pages e00346, XP093063497, ISSN: 2215-017X, DOI: 10.1016/j.btre.2019.e00346
DATABASE Nucleotide NCBI; 3 October 2019 (2019-10-03), "Cellvibrio sp. KY-GH-1 chromosome, complete genome", XP009545990, Database accession no. CP031728.1
KWON MIJUNG, JANG WON YOUNG, KIM GEON MIN, KIM YOUNG HO: "Characterization and Application of a Recombinant Exolytic GH50A β-Agarase from Cellvibrio sp. KY-GH-1 for Enzymatic Production of Neoagarobiose from Agarose", ACS OMEGA, ACS PUBLICATIONS, US, vol. 5, no. 45, 17 November 2020 (2020-11-17), US , pages 29453 - 29464, XP093063503, ISSN: 2470-1343, DOI: 10.1021/acsomega.0c04390
JANG WON YOUNG, HEE KYOUNG LEE AND YOUNG HO KIM: "Characterization of exolytic GH50A β-agarase and GH117A α-NABH involved in agarose saccharification of Cellvibrio sp. KY-GH-1 and possible application to mass production of NA2 and L-AHG", JOURNAL OF LIFE SCIENCE, vol. 31, no. 3, 1 March 2021 (2021-03-01), pages 356 - 365, XP093063501, DOI: 10.5352/JLS.2021.31.3.356
JANG WON YOUNG; KWON MI JUNG; KIM KI YUN; KIM YOUNG HO: "Enzymatic characterization of a novel recombinant 1,3-α-3,6-anhydro-L-galactosidase specific for neoagarobiose hydrolysis into monosaccharides", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER BERLIN HEIDELBERG, BERLIN/HEIDELBERG, vol. 105, no. 11, 31 May 2021 (2021-05-31), Berlin/Heidelberg, pages 4621 - 4634, XP037479732, ISSN: 0175-7598, DOI: 10.1007/s00253-021-11341-8
KWON GI HYUN, KWON MI JUNG, PARK JU EUN, KIM YOUNG HO: "Whole genome sequence of a freshwater agar-degrading bacterium Cellvibrio sp. KY-GH-1", BIOTECHNOLOGY REPORTS, ELSEVIER, vol. 23, 1 September 2019 (2019-09-01), pages e00346, XP093063497, ISSN: 2215-017X, DOI: 10.1016/j.btre.2019.e00346
DATABASE Nucleotide NCBI; 3 October 2019 (2019-10-03), "Cellvibrio sp. KY-GH-1 chromosome, complete genome", XP009545990, Database accession no. CP031728.1
KWON MIJUNG, JANG WON YOUNG, KIM GEON MIN, KIM YOUNG HO: "Characterization and Application of a Recombinant Exolytic GH50A β-Agarase from Cellvibrio sp. KY-GH-1 for Enzymatic Production of Neoagarobiose from Agarose", ACS OMEGA, ACS PUBLICATIONS, US, vol. 5, no. 45, 17 November 2020 (2020-11-17), US , pages 29453 - 29464, XP093063503, ISSN: 2470-1343, DOI: 10.1021/acsomega.0c04390
JANG WON YOUNG, HEE KYOUNG LEE AND YOUNG HO KIM: "Characterization of exolytic GH50A β-agarase and GH117A α-NABH involved in agarose saccharification of Cellvibrio sp. KY-GH-1 and possible application to mass production of NA2 and L-AHG", JOURNAL OF LIFE SCIENCE, vol. 31, no. 3, 1 March 2021 (2021-03-01), pages 356 - 365, XP093063501, DOI: 10.5352/JLS.2021.31.3.356
JANG WON YOUNG; KWON MI JUNG; KIM KI YUN; KIM YOUNG HO: "Enzymatic characterization of a novel recombinant 1,3-α-3,6-anhydro-L-galactosidase specific for neoagarobiose hydrolysis into monosaccharides", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER BERLIN HEIDELBERG, BERLIN/HEIDELBERG, vol. 105, no. 11, 31 May 2021 (2021-05-31), Berlin/Heidelberg, pages 4621 - 4634, XP037479732, ISSN: 0175-7598, DOI: 10.1007/s00253-021-11341-8
Attorney, Agent or Firm:
SUH, Jang-Chan et al. (KR)
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