Title:
GLUCOSE DEHYDROGENASE
Document Type and Number:
WIPO Patent Application WO/2003/106668
Kind Code:
A1
Abstract:
A glucose dehydrogenase using pyrroloquinoline quinone as a coenzyme, wherein the amino acid residues corresponding to the 349th to 377th residues of water-soluble PQQGDH derived from Acinetobacter calcoaceticus are replaced by other amino acid residues and whose inhibition constant (Ksi) is 200 mM or higher. The thus altered water-soluble PQQGDH is useful in the measuring of glucose in the presence of high-concentration glucose because of low level of substrate inhibition by glucose.
Inventors:
SODE KOJI (JP)
Application Number:
PCT/JP2003/007542
Publication Date:
December 24, 2003
Filing Date:
June 13, 2003
Export Citation:
Assignee:
SODE KOJI (JP)
International Classes:
C12N1/21; C12N9/04; C12N15/53; G01N33/66; (IPC1-7): C12N9/04; C12N15/53; C12N15/63; C12N1/21; C12Q1/32; C12Q1/54
Foreign References:
| JP2001346587A | 2001-12-18 | |||
| EP1167519A1 | 2002-01-02 |
Other References:
HIROSHI HAYADE ET AL.: "CSJ: The Chemical Society of Japan Dai 79 Shunki Nenkai - Koen Yokoshu II", THE CHEMICAL SOCIETY OF JAPAN, 15 March 2001 (2001-03-15), pages 897, XP002972209
OUBRIE A. ET AL.: "Structrure and mechanism of soluble quinoprotein glucose dehydrogenase", EMBO J., vol. 18, no. 19, 1999, pages 5187 - 5194, XP002952297
OUBRIE A. ET AL.: "The 1.7 A crystal structure of the apo from of the soluble quinoprotein glucose dehydrogenase from Acinetobacter calcoaceticus reveals a novel internal conserved sequence repeat", J. MOL. BIOL., vol. 289, no. 2, 1999, pages 319 - 333, XP002952296
OUBRIE A. ET AL.: "Structrure and mechanism of soluble quinoprotein glucose dehydrogenase", EMBO J., vol. 18, no. 19, 1999, pages 5187 - 5194, XP002952297
OUBRIE A. ET AL.: "The 1.7 A crystal structure of the apo from of the soluble quinoprotein glucose dehydrogenase from Acinetobacter calcoaceticus reveals a novel internal conserved sequence repeat", J. MOL. BIOL., vol. 289, no. 2, 1999, pages 319 - 333, XP002952296
Attorney, Agent or Firm:
Tanaka, Reiko (Kasumigaseki Building 36F 2-5, Kasumigaseki
3-chome, Chiyoda-k, Tokyo, JP)
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