Title:
GOLD NANOPARTICLE-SELEX BASED SCREENING METHOD FOR TARGET-SPECIFIC APTAMERS
Document Type and Number:
WIPO Patent Application WO/2021/029633
Kind Code:
A1
Abstract:
Screening of DNA/RNA aptamers that recognize target molecules (including biological molecules such as nucleic acids, lipids, sugars, proteins, and peptides, as well as hormones, low-molecular weight chemicals, toxins, ions, etc.) involves the systematic evolution of ligand exponential enrichment (SELEX). In general, the process of immobilizing target molecules on the surface of beads or a substrate is essentially required to perform SELEX. Also, since it is impossible to perform positive/negative monitoring that enables observation on whether an aptamer library is successfully binding with a target material in each round of the SELEX process, whether the SELEX process is properly performed is confirmed indirectly by analyzing the aptamers screened through multiple such rounds. In order to form a SELEX technique that is more convenient and simpler while addressing the aforesaid issues of existing techniques, the present invention proposes a novel SELEX technique using gold nanoparticles
Inventors:
KIM YOUNG PIL (KR)
LEE EUN SONG (KR)
KIM TAE WUK (KR)
LEE EUN SONG (KR)
KIM TAE WUK (KR)
Application Number:
PCT/KR2020/010534
Publication Date:
February 18, 2021
Filing Date:
August 10, 2020
Export Citation:
Assignee:
IUCF HYU (KR)
International Classes:
C12N15/10; C12N15/115
Foreign References:
| KR20180004503A | 2018-01-12 |
Other References:
LEE EUN-HEE; LEE SUNG KU; KIM MIN JUNG; LEE SEUNG-WOO: "Simple and rapid detection of bisphenol A using a gold nanoparticle-based colorimetric aptasensor", FOOD CHEMISTRY, vol. 287, 30 July 2019 (2019-07-30), pages 205 - 213, XP085624930, ISSN: 0308-8146, DOI: 10.1016/j.foodchem.2019.02.079
LEE EUN-SONG, KIM GAE BAIK, RYU SU-HYUN, KIM HYEON, YOO HYE HYUN, YOON MOON YOUNG, LEE JIN-WON, GYE MYUNG CHAN, KIM YOUNG-PIL: "Fluorescing aptamer-gold nanosensors for enhanced sensitivity to bisphenol A", SENSORS AND ACTUATORS B : CHEMICAL, vol. 260, 1 May 2018 (2018-05-01), pages 371 - 379, XP055781223, ISSN: 0925-4005, DOI: 10.1016/j.snb.2018.01.018
MINJOUNG JO; AHN JI-YOUNG; LEE JOOHYUNG; LEE SERAM; HONG SUN WOO; YOO JAE-WOOK; KANG JEEHYE; DUA POOJA; LEE DONG-KI; HONG SEUNGHUN: "Development of Single-Stranded DNA Aptamers for Specific Bisphenol A Detection", OLIGONUCLEOTIDES, vol. 21, no. 2, 1 April 2011 (2011-04-01), pages 85 - 91, XP055054675, ISSN: 1545-4576, DOI: 10.1089/oli.2010.0267
CHANG CHIA-CHEN; CHEN CHIE-PEIN; WU TZU-HENG; YANG CHING-HSU; LIN CHII-WANN; CHEN CHEN-YU: "Gold Nanoparticle-Based Colorimetric Strategies for Chemical and Biological Sensing Applications", NANOMATERIALS, vol. 9, no. 6, 861, 6 June 2019 (2019-06-06), pages 1 - 24, XP055777140, DOI: 10.3390/nano9060861
MICHAEL BLIND; MICHAEL BLANK: "Aptamer Selection Technology and Recent Advances", MOLECULAR THERAPY: NUCLEIC ACIDS, vol. 4, e233, 1 January 2015 (2015-01-01), pages 1 - 7, XP055342651, ISSN: 2162-2531, DOI: 10.1038/mtna.2014.74
ZHANG YANG, LAI BO, JUHAS MARIO: "Recent Advances in Aptamer Discovery and Applications", MOLECULES, vol. 24, no. 5, 941, 7 March 2019 (2019-03-07), pages 1 - 22, XP055781229, DOI: 10.3390/molecules24050941
LEE EUN-SONG, KIM GAE BAIK, RYU SU-HYUN, KIM HYEON, YOO HYE HYUN, YOON MOON YOUNG, LEE JIN-WON, GYE MYUNG CHAN, KIM YOUNG-PIL: "Fluorescing aptamer-gold nanosensors for enhanced sensitivity to bisphenol A", SENSORS AND ACTUATORS B : CHEMICAL, vol. 260, 1 May 2018 (2018-05-01), pages 371 - 379, XP055781223, ISSN: 0925-4005, DOI: 10.1016/j.snb.2018.01.018
MINJOUNG JO; AHN JI-YOUNG; LEE JOOHYUNG; LEE SERAM; HONG SUN WOO; YOO JAE-WOOK; KANG JEEHYE; DUA POOJA; LEE DONG-KI; HONG SEUNGHUN: "Development of Single-Stranded DNA Aptamers for Specific Bisphenol A Detection", OLIGONUCLEOTIDES, vol. 21, no. 2, 1 April 2011 (2011-04-01), pages 85 - 91, XP055054675, ISSN: 1545-4576, DOI: 10.1089/oli.2010.0267
CHANG CHIA-CHEN; CHEN CHIE-PEIN; WU TZU-HENG; YANG CHING-HSU; LIN CHII-WANN; CHEN CHEN-YU: "Gold Nanoparticle-Based Colorimetric Strategies for Chemical and Biological Sensing Applications", NANOMATERIALS, vol. 9, no. 6, 861, 6 June 2019 (2019-06-06), pages 1 - 24, XP055777140, DOI: 10.3390/nano9060861
MICHAEL BLIND; MICHAEL BLANK: "Aptamer Selection Technology and Recent Advances", MOLECULAR THERAPY: NUCLEIC ACIDS, vol. 4, e233, 1 January 2015 (2015-01-01), pages 1 - 7, XP055342651, ISSN: 2162-2531, DOI: 10.1038/mtna.2014.74
ZHANG YANG, LAI BO, JUHAS MARIO: "Recent Advances in Aptamer Discovery and Applications", MOLECULES, vol. 24, no. 5, 941, 7 March 2019 (2019-03-07), pages 1 - 22, XP055781229, DOI: 10.3390/molecules24050941
Attorney, Agent or Firm:
IPS PATENT FIRM (KR)
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