GPR40 AGONISTS FOR THE TREATMENT OF TYPE II DIABETES

CROSS-REFERENCE TO RELATED APPLICATIONS

This Application claims priority to United States Provisional Patent Application No. 62/203,969, filed August 12, 2015, which is hereby incorporated by reference in its entirety.

FIELD OF THE INVENTION

The present invention relates to novel compounds which are GPR40 agonists and are useful for the treatment of disorders that are affected by the modulation of the GPR40 receptor. The invention also relates to pharmaceutical compositions comprising one or more of such compounds, to processes to prepare such compounds and compositions, and to the use of such compounds or pharmaceutical compositions for the treatment of various diseases, syndromes and disorders, including Type II diabetes mellitus, obesity, obesity- related disorders, impaired glucose tolerance, insulin resistance, metabolic syndrome, other cardiovascular risk factors such as hypertension and cardiovascular risk factors related to unmanaged cholesterol and/ or lipid levels, osteoporosis, inflammation, and eczema, that are related to GPR.40 modulation.

BACKGROUND OF THE INVENTION

Diabetes is a rapidly expanding, devastating disease that currently affects over 371 million people in the world, with associated healthcare costs exceeding 470 billion dollars in the USA alone. There are two mam types of diabetes. Type 1 diabetes affects -10% of the patients and is characterized by complete insulinopenia due to autoimmune destruction of the insulm-secretmg pancreatic beta cells. Treatment of Type 1 diabetes requires insulin therapy. Type 2 diabetes affects -90% of the patients and is a polygenic syndrome with not only a hereditary component but also a strong environmental influence. It is caused by msulm resistance and defective insulin secretion. In most individuals, the pancreatic beta cell compensates for obesity-associated msulm resistance by expanding its functional mass

I and secretion of insulin. In a subset of -20% of obese subjects, beta cell compensation fails and Type 2 diabetes develops. Two major classes of type 2 diabetes drugs are insulin sensitizers (e.g. metformin, thiazolidinediones) and insulin secretogogues (e.g.

sulfonylureas, glmides, glucagon-like peptide- 1 (GLP-1 )-based drugs). Most of the recently approved drugs belong to the latter category and are based on the GLP-1 mechanism, either by pharmacologically enhancing GLP-1 levels (GLP-1 agonists) or by inhibiting the degradation of endogenous GLP-1 (dipeptidyl-peptidase 4 inhibitors). One advantageous feature of these drugs is that they only stimulate insulin secretion when blood glucose levels are elevated (as opposed to sulfonylureas and glinides), thus minimizing the risk of iatrogeni c hypoglycemia. A decade ago, the discovery of the G- protein-coupled receptor GPR40 as a fatty acid receptor specifically expressed in beta, cells and which stimulates glucose-dependent insulin secretion, sparked interest in the pharmaceutical industry as a potential therapeutic target to enhance insulin secretion in type 2 diabetes, in a manner similar to GLP-1 -based drugs. GPR40, also known as free fatty acid receptor 1 (FFARl), is one of a family of G-protein coupled receptors that, through receptor deorphanization studies, was shown to be endogenously activated by medium- to long-chain saturated and unsaturated fatty acids (~ C _12-2 o) (Brisco, et al., 2003, J, Biol. Chem., vol. 278: pgs 11303-1131 1 ; Itoh, et ai, 2003, Nature, vol. 422, pgs 173- 176; Kotarsky et al, 2003, Biochem. Biophys. Res. Comraun., vol. 301, pgs 406-410). In humans and rodents, although present in brain and enteroendocrine cells, its expression is particularly high in pancreatic beta, cells and enteroendocrine cells in the gut. Operating primarily through Ga _q/ n signaling, GPR40 activation of the beta cell leads to an increase in intracellular calcium levels, which in the presence of glucose, ultimately results in augmented insulin secretion. In enteroendocrine cells, GPR40 activation by fatty acids leads to stimulation of incretin secretion (Edfalk, et al., 2008, Diabetes, vol. 57, pgs 2280- 2287). Thus, in addition to directly promoting GSIS from islet beta cells, GPR40 activation in enteroendocrine cells provides an mdirect means of stimulating GSIS through the actions of released incretins. Because of the glucose dependency of GPR40-mediated effects on insulin secretion, selective activation of this receptor provides a unique potential therapeutic mechanism by which to treat the diabetic state with minimal risk of hypoglycemic incidents. Given the relatively restricted tissue expression pattern of GPR4Q, selective GPR40 receptor agonists may offer the additional advantage of providing an improved safety profile relative to the aforementioned therapeutic agents.

Thus, GPR40 receptor agonists of the present invention may provide a therapeutic benefit for the treatment of diabetes, particularly Type 2 diabetes, as well as diseases, syndromes and disorders, including obesity, obesity-related disorders, impaired glucose tolerance, insulin resistance, metabolic syndrome, other cardiovascular risk factors such as hypertension and cardiovascular risk factors related to unmanaged cholesterol and/ or lipid levels, osteoporosis, inflammation, and eczema.

SUMMARY OF THE INVENTION

present invention is directed to com ounds of Formula (I)

Formula (I)

wherein

X is S or O; provided that when X is O, Y is N;

Y is C(R ³ ) or N;

R ³ is hydrogen or methyl; or, when X is S, R ^' is hydrogen, methyl, or chloro; W is CH or N;

L is Π Μ.. -CI I CH-. or (Cl l <.) i-.-. ;

¾ is selected from the group consisting of phenyl, pyridin-4-yl, thienyi, benzothiophenvl, benzofuranvl, and indolyl; wherein said beiizothiophenyl, benzofuranyl, and indolyl are attached to the core (X)-(Y)-contaming ring via its benzo ring; and wherein R ^! is optionally independently substituted with one or two substituents selected from Ci_ ₄ alkyl, methoxy, fluoro, cyano, di(Ci- ₄ alkyl)amino, or trifluoromethyl;

K ^z is C3_ ₅ cycioalkyl, Cj ^alkyl, or cyano;

R ⁴ is hydrogen or chloro;

G is selected from the group consisting of d^alkyl, C _1-6 alkoxy, phenyl, unsubstituted C3 _-7 cycloalkyl, unsubstituted C3 _-7 cycloalkoxy, unsubstituted C^-cycloalkyi- methoxy, C _2-6 alk-l-en-l-yl, 3,3,3-trifluoropropoxy, 2,2,2-trifluoro-l -methyl-ethyl, (Ci- ₆ alkyl)thien-2-yl, and a substituent selected from gl to g6;

gl g2 g3 g4 g5 g6

or an enantiomer, diastereomer, or pharmaceutically acceptable salt form thereof.

The present invention also provides a pharmaceutical composition comprising, consisting of and/or consisting essentially of a pharmaceutically acceptable earner, a pharmaceutically acceptable excipient, and/or a pharmaceutically acceptable diluent and a compound of Formula (I), or a pharmaceutically acceptable salt form thereof.

Also provided are processes for making a pharmaceutical composition comprising, consisting of, and/or consisting essentially of admixing a compound of Formula (I), and a pharmaceutically acceptable carrier, a pharmaceutically acceptable excipient, and/or a pharmaceutically acceptable diluent.

The present invention further provides methods for treating or ameliorating a disease, syndrome, or condition in a subject, including a mammal and/or human in which the disease, syndrome, or condition is affected by the agonism of the GPR40 receptor, such as Type II diabetes, using a compound of Formula (I).

The present invention also is also directed to the use of any of the compounds described herein in the preparation of a medicament wherein the medicament is prepared for treating a disease or condition that is affected by the agonism of GPR40 receptor, selected from the group consisting of obesity, obesity related disorders, impaired oral glucose tolerance, insulin resistance, Type II diabetes mellitus, metabolic syndrome, metabolic syndrome X, dyslipidemia, elevated LDL, elevated triglycerides, obesity induced inflammation, osteoporosis and obesity related cardiovascular disorders, in a subject in need thereof.

Exemplifying the invention are methods of treating a disorder modulated by GPR40 receptor selected from the group consisting of obesity, obesity related disorders, impaired oral glucose tolerance, insulin resistance, Type II diabetes mellitus, metabolic syndrome, metabolic syndrome X, dyslipidemia, elevated LDL, elevated triglycerides, obesity induced inflammation, osteoporosis and obesity related cardiovascular disorders, comprising administering to a subject in need thereof a therapeutically effective amount of any of the compounds or pharmaceutical compositions described above.

In another embodiment, the present invention is directed to a compound of Formula (I) for use in the treatment of a disorder affected by the agonism of the GPR40 receptor selected from the group consisting of obesity, obesity related disorders, impaired oral glucose tolerance, insulin resistance, Type II diabetes mellitus, metabolic syndrome, metabolic syndrome X, dyslipidemia, elevated LDL, elevated triglycerides, obesity induced inflammation, osteoporosis and obesity related cardiovascular disorders.

In another embodiment, the present invention is directed to a composition comprising a compound of Formula (I) for the treatment of a disorder affected by the agonism of the GPR40 receptor, selected from the group consisting of obesity, obesity related disorders, impaired oral glucose tolerance, insulin resistance, Type II diabetes mellitus, metabolic syndrome, metabolic syndrome X, dyslipidemia, elevated LDL, elevated triglycerides, obesity induced inflammation, osteoporosis and obesity related cardiovascular disorders.

DETAILED DESCRIPTION OF THE INVENTION With reference to substituents, the term "independently" refers to the situation where when more than one substituent is possible, the substituents may be the same or different from each other.

The term "alkyl" whether used alone or as part of a substituent group, refers to straight and branched carbon chains having 1 to 8 carbon atoms. Therefore, designated numbers of carbon atoms (e.g., C _1-8 ) refer independently to the number of carbon atoms in an alkyl moiety or to the alkyl portion of a larger alkyi-containing substituent. In substituent groups with multiple alkyl groups such as,

groups of the dialkyiamino may be the same or different.

The term "alkoxy" refers to an -O-alkyl group, wherein the term "alkyl" is as defined above.

The terms "alkenyl" and "alkynyl" refer to straight and branched carbon chains having 2 to 8 carbon atoms, wherein an alkenyl chain contains at least one double bond and an alkynyl chain contains at least one triple bond.

The term "cycloalkyi" refers to saturated or partially saturated, monocyclic or poly cyclic hydrocarbon rings of 3 to 14 carbon atoms. Examples of such rings include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, and adamantyl.

The term "heterocyclyl" refers to a nonaromatic monocyclic or bicyclic ring system having 3 to 10 ring members that include at least 1 carbon atom and from 1 to 4 heteroatoms independently selected from N, O, and S. Included within the term heterocyclyl is a nonaromatic cyclic ring of 5 to 7 members in which 1 to 2 members are N, or a nonaromatic cyclic ring of 5 to 7 members in which 0, 1 or 2 members are N and up to 2 members are 0 or S and at least one member must be either N, O, or S; wherein, optionally, the ring contains 0 to 1 unsaturated bonds, and, optionally, when the ring is of 6 or 7 members, it contains up to 2 unsaturated bonds. The carbon atom ring members that form a heterocycle ring may be fully saturated or partially saturated. The term

"heterocyclyl" also includes two 5 membered monocyclic heterocycloalkyl groups bridged to form a bicyclic ring. Such groups are not considered to be fully aromatic and are not referred to as heteroaryl groups. When a heterocycle is bicyclic, both rings of the heterocycle are non-aromatic and at least one of the rings contains a heteroatom ring member. Examples of heterocycle groups include, and are not limited to, pyrrolinyl (including 2H-pyrrole, 2-pyrrolinyl or 3-pyrrolmyl), pyrrolidinyl, imidazolinyl, imidazolidinyl, pyrazolinyl, pyrazolidinyl, piperidinyl, morpholinyl, thiomorphoimyl, and piperazmyl. Unless otherwise noted, the heterocycle is attached to its pendant group at any heteroatom or carbon atom that results in a stable structure.

The term "aryi" refers to an unsaturated, aromatic monocyclic or bicyclic ring of 6 to 10 carbon members. Examples of aryl rings include phenyl and naphthalenyi. The term "heteroaiyi" refers to an aromatic monocyclic or bicyclic aromatic ring system having 5 to 10 ring members and which contains carbon atoms and from 1 to 4 heteroatoms independently selected from the group consisting of N, O, and S. Included within the term heteroaiyi are aromatic rings of 5 or 6 members wherein the ring consists of carbon atoms and has at least one heteroatom member. Suitable heteroatoms include nitrogen, oxygen, and sulfur. In the case of 5 membered rings, the heteroaiyi ring preferably contains one member of nitrogen, oxygen or sulfur and, in addition, up to 3 additional nitrogens. In the case of 6 membered rings, the heteroaryl ring preferably contains from 1 to 3 nitrogen atoms. For the case wherein the 6 membered ring has 3 nitrogens, at most 2 nitrogen atoms are adjacent. Examples of heteroaryl groups include fur}'], thienyl, pyrrolyl, oxazolyl, thiazolyl, imidazolyl, pyrazolyl, isoxazolyl, isothiazolyl, oxadiazolyl, triazolyl, thiadiazolyl, pyridinyl, pyridazinyl, pyrimidinyl, pyrazinyl, mdolyl, isoindolyl, benzofuryl, benzothienyl, indazolyl, benzimidazolyl, benzothiazolyl, benzoxazolyl, benzisoxazolyl, benzothiadiazolyl, benzotriazolyl, quinolinyi, isoquinolinyl and quinazolinyl. Unless otherwise noted, the heteroaryl is attached to its pendant group at any heteroatom or carbon atom that results in a stable structure.

The term "halogen" or "halo" refers to fluorine, chlorine, bromine and iodine atoms.

The term "formyl" refers to the group -C(=0)H.

The term "oxo" refers to the group (==()). Whenever the term "alkyl" or "aryi" or either of their prefix roots appear in a name of a substituent (e.g., arylalkyl, alkylamino) the name is to be interpreted as including those limitations given above for "alkyl" and "aryi." Designated numbers of carbon atoms (e.g., Ci-Ce) refer independently to the number of carbon atoms in an alkyl moiety, an aryi moiety, or in the alkyl portion of a larger substituent in which alkyl appears as its prefix root. For alkyl and alkoxy substituents, the designated number of carbon atoms includes all of the independent members included within a given range specified. For example Cj.6 alkyl would include methyl, ethyl, propyl, butyl, pentyi and hexyl individually as well as sub-combinations thereof (e.g., C1-2, C _{1 -3} , C1-4, Ci _-5 , C _2-6 , C _3- 6, C4-6, C5-6, C2-5, etc.).

In general, under standard nomenclature rules used throughout this disclosure, the terminal portion of the designated side chain is described first followed by the adjacent functionality toward the point of attachment. Thus, for example, a "Ci-Ce alkyicarbonyl" substituent refers to a group of the formula:

O

H

^• C— C _r C ₆ alkyl

The substituent "-(¾()-" is oriented such that the oxygen atom is covaiently bound to the W-containing ring.

The term "R" at a stereocenter designates that the stereocenter is purely of the R- configuration as defined in the art; likewise, the term "S" means that the stereocenter is purely of the ^-configuration. As used herein, the terms "*R" or "*S" at a stereocenter are used to designate that the stereocenter is of pure but unknown configuration. As used herein, the term "RS" refers to a stereocenter that exists as a mixture of the R- and S- configurations. Similarly, the terms "*RS" or "*SR" refer to a stereocenter that exists as a mixture of the R- and 5'-configurations and is of unknown configuration relative to another stereocenter within the molecule.

Compounds containing one stereocenter drawn without a stereo bond designation are a mixture of two enantiomers. Compounds containing two stereocenters both drawn without stereo bond designations are a mixture of four diastereomers. Compounds with two stereocenters both labeled "RS" and drawn with stereo bond designations are a two- component mixture with relative stereochemistry as drawn. Compounds with two stereocenters both labeled "*RS" and drawn with stereo bond designations are a two- component mixture with relative stereochemistry unknown. Unlabeled stereocenters drawn without stereo bond designations are a mixture of the R- and ^-configurations. For unlabeled stereocenters drawn with stereo bond designations, the absolute stereochemistry is as depicted.

Thus a compound of the present invention can be an i-enantionier, an R- enantiomer, or a mixture of both an S-enantiomer and an i?-enantiomer.

In one embodiment, the compound of the present invention is an S-enantiorner.

Unless otherwise noted, it is intended that the definition of any substituent or variable at a particular location in a molecule be independent of its definitions elsewhere in that molecule. It is understood that substituents and substitution patterns on the compounds of the present invention can be selected by one of ordinary skill in the art to provide compounds that are chemically stable and that can be readily synthesized by techniques known in the art as well as those methods set forth herein.

The term "subject" refers to an animal, preferably a mammal, most preferably a human, who has been the object of treatment, observation or experiment.

The term "therapeutically effective amount" refers to an amount of an active compound or pharmaceutical agent, including a compound of the present invention, which elicits the biological or medicinal response in a tissue system, animal or human that is being sought by a researcher, veterinarian, medical doctor or other clinician, which includes alleviation or partial alleviation of the symptoms of the disease, syndrome, condition, or disorder being treated.

The term "composition" refers to a product that includes the specified ingredients in therapeutically effective amounts, as well as any product that results, directly, or indirectly, from combinations of the specified ingredients in the specified amounts. The term "GPR40 agonist" is intended to encompass a compound that interacts with GPR40 to substantially increase its catalytic activity, thereby increasing the concentrations of its substrate(s).

The term "GPR40-modulated" is used to refer to the condition of being affected by the modulation of the GPR40 receptor, including but not limited to, the state of being mediated by the GPR40 receptor, for the treatment of a disease or condition such as obesity or Type II diabetes.

The term "agonism of the GPR40 receptor" is used to refer to the state of interaction of a compound of the present invention with said receptor resulting in a substantial increase in intracellular signaling, thereby increasing the consequential biological effects.

As used herein, unless otherwise noted, the term "disorder modulated by the GPR40 receptor" shall mean any disease, disorder or condition characterized in that at least one of its characteristic symptoms is alleviated or eliminated upon treatment with a GPR40 receptor agonist. Suitable examples include, but are not limited to obesity, obesity related disorders, impaired oral glucose tolerance, insulin resistance, Type II diabetes mellitus, metabolic syndrome, metabolic syndrome X, dyslipidemia, elevated LDL, elevated triglycerides, obesity induced inflammation, osteoporosis and obesity related

cardiovascular disorders; preferably, obesity, insulin resistance, Type II diabetes mellitus, dyslipidemia or metabolic syndrome X; more preferably, Type II diabetes mellitus or dyslipidemia.

As used herein unless otherwise noted, the term "obesity related cardiovascular disorders" shall mean any cardiovascular disease, disorder or condition in which obesity or diabetes (preferably, Type Π Diabetes) has a role in the initiation or exacerbation of said disorder or condition. Suitable examples include, but are not limited to, hypertension, atherosclerosis and cardiac fibrosis.

As used herein, unless otherwise noted, the term "affect" or "affected" (when referring to a disease, syndrome, condition or disorder that is affected by agonism of the GPR40 receptor) includes a reduction in the frequency and or severity of one or more symptoms or manifestations of said disease, syndrome, condition or disorder; and / or include the prevention of the development of one or more symptoms or manifestations of said disease, syndrome, condition or disorder or the development of the disease, condition, syndrome or disorder.

The compounds of the instant invention are useful in methods for treating or ameliorating a disease, a syndrome, a condition or a disorder that is affected by the agonism of the GPR Q receptor. Such methods comprise, consist of and/ or consist essentially of administering to a subject, including an animal, a mammal, and a human in need of such treatment, amelioration and / or prevention, a therapeutically effective amount of a compound of Formula (I) or an enantiomer, diastereomer, solvate or pharmaceutically acceptable salt thereof.

In particular, the compounds of Formula (I) or an enantiomer, diastereomer, solvate or pharmaceutically acceptable salt thereof, are useful for treating or ameliorating diseases, syndromes, conditions, or disorders such as obesity and type II diabetes.

More particularly, the compounds of Formula (I) or an enantiomer, diastereomer, solvate or pharmaceutically acceptable salt thereof!, are useful for treating or ameliorating type II diabetes, comprising administering to a subject in need thereof a therapeutically effective amount of a compound of Formula (I) or an enantiomer, diastereomer, solvate or pharmaceutically acceptable salt thereof as herein defined.

Embodiments of the present invention include a compound of Formula (I)

Formula (I)

wherein

a) X is S:

b) X is O and Y is N; L is -CH2O-, (Z) (Ί I Π 1 -. or -(CH ₂ ) ₂ - ;

L is C! I O ;

R ^¾ is selected from the group consisting of phenyl, pyridin-4-yl, thienyl, benzothiophenyl, benzofuranyl, and indolyl; wherein said benzothiophenyi, benzofuranyl, and indolyl are attached to the core (X)-(Y) containing ring via its benzo ring: and wherein R ^! is optionally independently substituted with one or two substituents selected from methyl, methoxy, or fluoro;

R ¹ is selected from the group consisting of phenyl, pyridin-4-yl, and thienyl;

wherein R ^¾ is optionally independently substituted with one or two substituents selected from methyl, methoxy, or fluoro;

R ^¾ is selected from the group consisting of 2-fluoro-5-methoxy-phenyl, 5-fluoro-2- methoxy-pyrid-4-yl, 6-methoxybenzothiophen-4-yl, thien-3-yl, 3- (dimethylamino)phenyl, 2-fluoro-4-methoxy-phenyl, phenyl, 2-fluorophenyl, 2- methoxyphenyl, 3,5-dimethoxyphenyl, lH-indol-4-yl, benzofuran-4-yl, 3- methoxyphenyl, 5-methoxypyrid-3~yl, 2-methoxypyrid-4~yl, and 5-cyano-2-fluoro- phenyl;

R is C3 _-5 cycloalkyl;

R ² is cyclopropyl;

G is selected from the group consisting of Ci-ealkoxy, phenyl, unsubstttuted C _{3- 7} cycloalkyl, unsubstttuted C ₃ - ₇ cycloalkoxy, unsubstttuted C ₃ - ₇ cycloalkyI-methoxy, C ₂ ^alk-l -en-l -yl, 3,3,3-trifluoropropoxy, (C _1-4 alkyl)thien-2-yl, and a substituent selected from gl to g6 ;

gl g2 g3 g4 g5 g6

G is selected from the group consisting of C^alkoxy, unsubstituted C _3-7 cycloalkyl, unsubstituted C _3- ?cycloalkoxy, unsubstituted C _3-7 cycloalkyl-methoxy, C _2- 6alk-l -en- 1 -yl, 5-(C _1-4 alkyl)thien-2-yl, and a substituent selected from gl to gS;

G is selected from the group consisting of C _1-4 alkoxy, unsubstituted C3- cycloalkyl unsubstituted C3. ₇ cycloalkoxy, unsubstituted C3 _-7 cycloalkyl-methoxy, (\ _(l aik- l -en -yl, 5-(C _1-4 alkyl)thien-2-yl and a substituent selected from g2, g4, or g5 ;

G is selected from the group consisting of 2-methyl-propyl, ethoxy, isopropyloxy, 2-methyl-propyloxy, cyclopropyl, cyclobutyloxy, cyclopentyloxy, cyclohexyloxy, cyclopropylmethoxy, 2-methyl-prop-l -en-l -yl, phenyl, 5-(methyl)thien-2-yl, 5-(t- butyl)thien-2-y], 3-(methyl)thien-2-yl, 2,2,2-trifluoro-l -methyl-ethyl, 3,3,3- trifluoropropyloxy, and a substituent selected from gl to g5;

and any combination of embodiments a) through m) above, provided that it is understood that combinations in which different embodiments of the same substituent would be combined are excluded;

or an enantiomer, diastereomer, or pharmaceutically acceptable salt form thereof. An embodiment of the present invention includes a compound of Formula (I)

Formula (I)

wherein

X is S or O; provided that when X is O, Y is N;

Y i s C( - ) or N;

R ³ is hydrogen or methyl ; or when X is S, R ³ is hydrogen, methyl, or chloro;

W is CH or N;

L is -CH2O-, -CM Π Ι-. or -(CH ₂ )2-- ;

R ¹ is selected from the group consisting of phenyl, pyridin-4-yl, and thienyl;

wherein R ^! is optionally independently substituted with one or two substituents selected from methyl, methoxy, or fluoro;

R ² is C3- ₅ cycloalkyl;

R ⁴ is hydrogen or chloro;

G is selected from the group consisting of C^alkoxy, phenyl, unsubstituted C _3- 7cycloalkyl, unsubstituted C3 _-7 cycloalkoxy, unsubstituted C _3- 7cycloalkyl-methoxy, < ^' ·.,·,;· Ik- -en-l-yl, 5-(C _{1 -4} alkyl)thien-2-yl, and a substituent selected from gl to g6 ;

gl g2 g3 g4 g5 g6

or an enantiomer, diastereomer, or pharmaceutically acceptable salt form thereof.

An embodiment of the present invention includes a compound of Formula (I)

Formula (I)

wherein

X is O;

Y is N;

W is CH or N;

L is Π ! '()-. -( I I i l l-, or (CM _. - _. b ;

R ¹ is selected from the group consisting of phenyl, pyridin-4-yl, or thienyl; wherein R ¹ is optionally independently substituted with one or two substituents selected from methyl, methoxy, or f!uoro;

R ² is C3- ₅ cycloalkyl;

R ⁴ is hydrogen or chloro;

G is selected from the group consisting of d^alkoxy, unsubstituted Cs.ycycloalky], unsubstituted C3 _-7 cycloalkoxy, unsubstituted C3. ₇ cycloalkyl-meth.oxy, C _2-6 alk- l -en-l -yl, -(C _1-4 alkyl)thien-2-yl, and a substituent selected from gl to g5;

or an enantiomer, diastereomer, or pharmaceutically acceptable salt form thereof.

An embodiment of the present invention includes a compound of Formula (I)

Formula (I)

wherein

X is O;

Y i s N;

W is CH or N;

L is -CH2O-, -CH=CH~, or ~(CH ₂ ) ₂ - ;

R ¹ is selected from the group consisting of phenyl, pyridin-4-yl, and thienyl;

wherein R ¹ is optionally independently substituted with one or two substituents selected from methyl, methoxy, or fluoro;

R is cyclopropyl;

R ⁴ is hydrogen or chloro;

G is selected from the group consisting of unsubstituted C3. ₇ cycloa.lkyl, unsubstituted C3 _-7 cycloalkoxy, unsubstituted C3 _-7 cycloalkyl-methoxy, C _2-6 alk-l-en-l -yl, 5- (C _1- 4alkyl)thien-2-yl and a substituent selected from g2, g4, or g5 ;

g2 g4 g5

or an enantiomer, diastereomer, or pharmaceutically acceptable salt form thereof.

An embodiment of the present invention includes a compound of Formula

Formula (I)

wherein

X is S or O; provided that X is O when Y is N;

Y is C(R ³ ) or N;

R ³ is hydrogen or methyl; or when X is S, R ³ is hydrogen, methyl, or chloro;

W is CH or N;

L is -CH2O-, - ⁽ I I i l l-. or -(CH ₂ )i- ₂ - ;

R ¹ is selected from the group consisting of phenyl, pyridin-4-yl, thienyl, benzothiophenyl, benzofuranyl, and indolyl; wherein said benzothiopheny], benzofuranyl, and indolyl are attached to the core (X)-(Y) containing ring via its benzo ring; and wherein R ^l is optionally independently substituted with one or two substituents selected from Ci_ ₄ alkyl, methoxy, fluoro, cyano, or di(C _1-4 alkyl)amino;

R ^" is C3- ₅ cycloalkyl;

R ⁴ is hydrogen or chloro;

G is selected from the group consisting of 2-methyl-propyl, ethoxy, isopropyloxy. 2-methyl-propyloxy, cyclopropyl, cyclobutyloxy, cyclopentyloxy, cyciohexyloxy, cyclopropylmethoxy, 2-methyl-prop-l-en-l-yl, phenyl, 5-(methyl)thien-2-yl, 5-(t- butyi)thien-2-yi, 3-(methyl)thien-2-yl, 2,2,2-trifluoro-l -methyl-ethyl, 3,3,3- trifluoropropyloxy, and a substituent selected from gl to g5;

enantiomer, diastereomer, or pharmaceutically acceptable salt form thereof. An embodiment of the present invention includes a compound of Formula (I)

Formula (I)

wherein

X is S or O; provided that X is O when Y is N;

Y is C(R ³ ) or N;

R ^J is hydrogen; or when X is S, R ^J is hydrogen, methyl, or chloro;

W is CH or N;

L is (Ί ! ·()-. ( ·.} -CH=CH-, or --(CH ₂ ) ₂ - ;

R is selected from the group consisting of 2-fluoro-5-methoxy-phenyl, 5-fluoro-2- methoxy-pyrid-4-yl, 6-methoxybenzothiophen-4-yl, thien-3-yl, 3-(dimethylamino)phenyl, 2-fluoro-4-methoxy-phenyl, phenyl, 2-fluorophenyl, 2-methoxyphenyl, 3,5- dimethoxyphenyi, lH-indol-4-yl, benzofuran-4-yl, 3-methoxyphenyl, 5-methoxypyrid-3- yl, 2-methoxypyrid-4-yl, and 5-cyano-2-fluoro-phenyl;

R is cyciopropyl;

R ⁴ is hydrogen or chloro;

G is selected from the group consisting of 2-methyl-propyl, cyciopropyl, ethoxy, isopropyloxy, 2-methyl-propyloxy, cyclobutyloxy, cyciopentyloxy, cyclohexyloxy, cyclopropylmethoxy, 2-methyl-prop-l-en-l -yl, phenyl, 3-(methyl)thien-2-yl, 5- (methyl)thien-2-yl, 5-(t-butyl)thien-2-yl, 2,2,2-trifluoro-l -methyl-ethyl, 3,3,3- trifluoropropyloxy, and a substituent selected from gl to g5;

or an enantiomer, diastereomer, or pharmaceutically acceptable salt form thereof.

In one embodiment of the present invention, the compound of Formula (I) enantiomer

Additional embodiments of the present invention include compounds of Formula (I) as herein defined, or an enantiomer, diastereomer, solvate, or a pharmaceutically acceptable salt form thereof, wherein the substituents selected from one or more of the variables defined herein (e.g. X, Y, W, R ^¾ , R ² , R ³ , R ⁴ , and G) are independently selected to be any individual substituent or any subset of substituents from those exemplified in the listing in Table 1 , below.

Table 1.

Cpd X Y W L R ¹ R ² R ⁴ G

2-fluoro-5-methoxy- (35)-3-

1 0 N CH C! )- II

phenyl 8 cyclopropyl

5-fluoro-2-methoxy- (3S)-3-

2 0 N CH -CH ₂ 0- H g5

4-pyridyl cyclopropyl

2-fluoro- 5 -meth oxy- (3S)-3-

3 0 N CH -CH ₂ 0- II

phenyl cvclopropyl g5

5-fluoro-2-methoxy- (35)-3-

4 0 N CH -CH ₂ 0~ H g4

4-pyridyl cyclopropyl

5 0 N CH -CH ₂ 0- 2-fluoro-5-methoxy- (3S)-3- II g2 Cpd X Y W L R ¹ R ² R ⁴ G

phenyl cyclopropy 1

(E)~ 2-fluoro- 5 -methoxy-

6 0 N CH cyclopropyl H cyclopropyl

CH=CH- phenyl

2-fl uor o- 5 -m ethoxy-

7 0 N CH -(CH ₂ ) ₂ - cyclopropy! II cyclopropyl phenyl

5-fluoro-2-methoxy- (3S)-3-

8 0 N CH -CH2O- H g2

4-pyridyl cyclopropyl

2-fluoro- 5 -meth oxy- 5-

9 0 N N CH2O- cyclopropyl

phenyl Cl gl

6-methoxybenzo (35)-3-

10 s CH CH -CH ₂ O- H

thiophen-4-yl cyclopropyl gl

2-fluoro- 5 -methoxy- (35)-3-

11 0 N CH -CH ₂ 0- II 83 phenyl cyclopropyl

5 -fluoro- 2-methoxy- (3S)-3-

12 0 N CH -CH2O- H isobutyl

4-pyridyl cyclopropyl

s 2-fluoro- 5 -methoxy- ^• ; 3.s ;-3-

13 CH CH CH2O- II

phenyl cyclopropyl gl s 2-fluoro- 5 -methoxy- (35)-3-

14 CH CH -CH ₂ O- H cyclopropyl phenyl cyclopropyl

2-fluoro- 5 -methoxy- (35)-3-

15 0 N CH -CH2O- H

phenyl cyclopropyl gl s (3S)-3-

16 CH CH CH ₂ O- thien-3-yl II

cyclopropyl gl

3 -(dimethy lamino) (3S)~3~

17 s CH CH -CH2O- H

phenyl cyclopropyl gl

2-fluoro-4-methoxy- (3S)-3-

18 s CH CH CH2O- II

phenyl cyclopropyl gl s 2-fluoro-5-methoxy- (3S -3-

19 CH CH -CH2O- H trifluoro-1- phenyl cyclopropyl

methyl-ethyl

(35)-3-

20 s CH CH -CH ₂ 0- phenyl II

cyclopropyl gl s (3S)~3~

21 CH CH -CH2O- 2-fluorophenyl H

cyclopropyl g

•; 3.s ;-3"

22 s CH CH CH2O- 2-methoxyphenyl II

cvclopropyl gl

23 s 3,5- (35)-3-

CH CH -CH ₂ O- H

dimethoxypheny 1 cyclopropyl gl s (35)-3-

24 CH CH CH ₂ O- lH-mdol-4-yi II

cyclopropyl gl

(3S)-3-

25 s CH CH CH ₂ O- benzofuran-4-yl II

cyclopropyl gl Cpd X Y W L R ¹ R ² R ⁴ G

(3S)-3-

26 S CH CH -CH ₂ 0- 3-methoxyphenyl II

cyclopropyl gl s 5-fluoro-2-methoxy- (35)-3-

27 CH CH -CH ₂ 0- H

4-pyridyl cyclopropyl gi s (3S)-3-

28 CH CH -CH ₂ 0- 5 -raethoxy pynd- 3 -y 1 II

cyclopropyl gl s C- 2-fluoro-5-methoxy- (35)-3-

29 CH -CH ₂ 0- H trifluoro-1-

(Cl) phenyl cyclopropyl

methvl-ethvl

2-fluoro-5-methoxy- ^• ;3.s;-3-

30 s N CH CH ₂ 0- II

phenyl cyclopropyl gl

(35)-3-

31 s CH CH -CH ₂ 0~ 2-methoxypyrid-4-yl H

cyclopropy] gl

2-fluoro-5-methoxy- (35)-3- 5 -methyl

32 0 N CH C! )- II

phenyl cyclopropyl thien-2-vl

2-fl uoro- 5 -m ethoxy- (3S -3- 3 -methyl

33 0 N CH -CH ₂ 0- H

phenyl cyclopropyl thien-2-yl

2-fluoro- 5 -methoxy- ^• ;3.s;-3-

34 0 N CH CH ₂ 0- II phenyl phenyl cyclopropyl

2-fluoro- 5 -meth oxy- (3S)-3-

35 0 N CH -CH ₂ 0- II isobutyl phenyl cyclopropyl

2-fluoro- 5 -methoxy- (35)-3- 2-methyl

36 0 N CH -CH ₂ 0- H

phenyl cyclopropyl prop-l-enyl

2-fluoro- 5 -meth oxy- (3S)-3-

37 0 N CH -CH ₂ 0- II cyclopropyl phenyl cyclopropyl

s 5-cyano-2-fluoro- (3S)-3-

38 CH CH -CH ₂ 0- H

phenyl cyclopropyl g

2-fluoro- 5 -meth oxy- (3S)-3-

39 0 N CH -CH ₂ 0- II isobutoxy phenyl cvclopropyl

2-fluoro- 5 -methoxy- (35)-3- cyclo

40 0 N CH -CH ₂ 0~ H

phenyl cyclopropyl penlyloxy

2-fluoro- 5 -methoxy- (35)-3- cyclopropyl

41 0 N CH -CH ₂ 0- II

phenyl cyclopropyl methoxy

2-fluoro-5-methoxy- (3S)-3- isopropyl

42 0 N CH -CH ₂ 0- H

phenyl cyclopropyl oxy

2-fluoro- 5 -methoxy- ^• ;3.s;-3- cyclobutyl

43 0 N CH CH ₂ 0- II

phenyl cyclopropyl oxy

2-fluoro- 5 -methoxy- (35)-3- 5-tert-b tyl-

45 0 N CH -CH ₂ 0~ H

phenyl cyclopropyl thien-2-vl

2-fluoro- 5 -methoxy- (35)-3- cyclohexyl

46 0 N CH -CH ₂ 0- II

phenyl cyclopropyl oxy Cpd X Y W L R ¹ R ² R ⁴ G

5-fluoro-2- (3S)-3-

47 0 N CH -CH ₂ 0- II isobutoxy methoxypyri d-4-y 1 cyclopropyl

5-fluoro-2- (3S)-3- cyclohexyl

48 0 N CH -CH ₂ 0- H

methoxypyrid-4-yl cyclopropyl oxy

3,3,3-

2-fluoro- 5 -meth oxy- (3S)-3-

49 0 N CH -CH ₂ 0- II trifluoro phenyl cyclopropyl

propyloxy

2-fluoro-5-methoxy- (3S)-3-

50 0 N CH -CH ₂ 0- H ethoxy phenyl cyclopropyl

5-fluoro-2- (3S)-3-

51 0 N CH CH ₂ 0- II ethoxy methoxypyrid-4-yl cvclopropyl

5-fluoro-2- (35)-3- 2 -methyl

52 0 N CH -CH ₂ 0~ H

methoxypyri d-4-yl cyclopropyl prop-l-enyl

Additional embodiments of the present invention include the following compounds of Formula (I) as herein defined, or an enantiomer, diastereomer, solvate, or a

pharmaceutically acceptable salt form thereof, exemplified in the listing in Table 2.

Table 2.



Formula (I)

is selected from the group consisting of

Cpd 1, (3S -3-cyclopropyl-3-[3-[[5-(2-fluoro-5-methoxy-phenyl)-4-(3,3,4 ,4- tetramethylcyclopentoxy)isoxazol-3-yl]methoxy]phenyl]propano ic acid;

Cpd 2, (3S -3-cyclopropyl-3-[3-[[5-(5-fluoro-2-methoxy-4-pyridyl)-4-(3, 3,5,5- tetramethylcyclohexoxy)isoxazol-3-yl]methoxy]phenyl]propanoi c acid;

Cpd 3, (35)-3-cyclopropyl-3-[3-[[5-(2-fluoro-5-methoxy-phenyl)-4-(3 ,3,5,5- tetramethylcyclohexoxy)isoxazol-3-yl]methoxy]phenyl]propanoi c ac d;

Cpd 4, (35)-3-cyclopropyl-3-[3-[[5-(5-fluoro-2-methoxy-4-pyridyl)-4 -(3,3,4,4- tetramethylcyclopentoxy)isoxazol-3 -y 1 ] methoxy ] phenyl jpropanoic aci d;

Cpd 5, (35)-3-cyclopropyl-3-[3-[[4-[(3,3-dimethylcyclobutyl)methoxy ]-5-(2-fluoro-5- methoxy-phenyl)isoxazol-3-yl]methoxy]phenyl]propanoic acid;

Cpd 6, 3-cyclopropyl-3-[4-[(E)-2-[4-cyclopropyl-5-(2-fluoro-5-metho xy-phenyl)isoxazol- 3-yl]vinyl]phenyl]propanoic acid;

Cpd 7, 3-cyclopropyl-3-[3-[2-[4-c>'clopropyl-5-(2-fluoro-5-metho xy-phenyl)isoxazol-3- y 1 ] ethyl] pheny 1 ] propanoic aci d;

Cpd 8, (3»S)-3-cyclopropyl-3-[3-[[4-[(3,3-dimethylcyclobutyl)metho xy]-5-(5-fluoro-2- methoxy-4-pyridyl)isoxazol-3-yl]methoxy]phenyl]propanoic acid;

Cpd 9, 3-[5-chloro-2-[[4-(5,5-dimethylcyclopenten-l -yl)-5-(2-fluoro-5-methoxy- phenyl)isoxazol-3-yl]methoxy]-4-pyridyl]-3-cyclopropyl-propa noic acid;

Cpd 10, (35)-3-cyclopropyl-3-[3-[[4-(5,5-dimethylcyclopenten-l-yl)-5 -(6- methoxybenzothiophen-4-yl)-3-thienyl]methoxy]phenyl]propanoi c acid;

Cpd 11, (3^)-3-cyclopropyl-3-[3-[[5-(2-fluoro-5-methoxy-phenyl)-4-[( 2,2,3,3- tetramethylcyclopropyl)methoxy]isoxazol-3-yl]methoxy]phenyl] propanoic acid; -3-cyclopropyl-3-[3-[[5-(5-fluoro-2-methoxy-4-pyridyl)-4-iso butyl-isoxazol- 3-yl]methoxy]phenyl]propanoic acid;

Cpd 13, (35)-3-cyclopropyl-3-[3-[[4-(5,5-dimethylcyclopenten-l -yl)-5-(2-fluoro-5- methoxy-phenyl)-3-thienyl]methoxy]phenyl]propanoic acid;

Cpd 14, (35)-3-cyclopropyl-3-[3-[[4-cyclopropyl-5-(2-fluoro-5-methox y-phenyl)-3- thienyl]methoxy]phenyl]propanoic acid;

Cpd 15, (35)-3-cyclopropyl-3-[3-[[4-(5,5-dimethylcyclopenten-l -yl)-5-(2-fluoro-5- methoxy-phenyl)isoxazol-3-yl]methoxy]phenyl]propanoic acid;

Cpd 16, (35)-3-cyclopropyl-3-[3-[[4-(5,5-dimethylcyclopenten-l-yl)-5 -(3-thienyl)-3- thienyl]methoxy]phenyl]propanoic acid;

Cpd 17, (3S)- 3 -cyclopropyl-3 - [3 - [ [5 - [3 -(dimethylamino)phenyl] -4-(5 , 5~

dimethylcyclopenten-l-yl)-3-thienyl]methoxy]phenyl]propanoic acid;

Cpd 18, (35)-3-cyclopropyl-3-[3-[[4-(5,5-dimethylcyclopenten-l -yl)-5-(2-fluoro-4- methoxy-phenyl)-3-thienyl]methoxy]phenyl]propanoic acid;

Cpd 19, (35)-3-cyclopropyl-3-[3-[[5-(2-fluoro-5-methoxy-phenyl)-4-(2 ,2,2-trifluoro-l- methyl-ethyl)-3-thienyl]methoxy]phenyl]propanoic acid;

Cpd 20, (3 _i S ^' )-3-cyclopropyl-3-[3-[[4-(5,5-dimethylcyclopenten-l -yl)-5-phenyl-3- thienyl]methoxy]phenyl]propanoic acid;

Cpd 21, (35)-3-cyclopropyl-3-[3-[[4-(5,5-dimethylcyclopenten-l -yl)-5-(2-fluorophenyl)-3- thienyl]methoxy]phenyl]propanoic acid;

Cpd 22, (3 _i S ^' )-3-cyclopropyl-3-[3-[[4-(5,5-dimethylcyclopenten- 1 -yl)-5-(2- methoxyphenyl)-3-thienyl]methoxy]phenyl]propanoic acid;

Cpd 23, (3 V3-cyclopropyl-3-f3-ff5-(3,5-dimethoxyphenyl)-4-(5,5-dimethy lcyclopenten- 1 -yl)-3-thienyl]methoxy ]phenyl]propanoic acid;

Cpd 24, (3 V3-cyclopropyl-3-[3-[[4-(5,5-dimethylcyclopenten-l-yl)-5-(lH -indol-4-yl)-3- thienyl]methoxy]phenyl]propanoic acid;

Cpd 25, (3 V3-f3-ff5-(benzofuran-4-yl)-4-(5,5-dimethylc>'clopenten-l -yl)-3- thienyl]methoxy]phenyl]-3-cyclopropyl-propanoic acid; -3-cyclopropyl-3-[3-[[4-(5,5-dimethylcyclopenten-l-yl)-5-(3- methoxyphenyl)-3-thienyl]methoxy]phenyl]propanoic acid;

Cpd 27, (3»S)-3-cyclopropyl-3-[3-[[4-(5,5-dimethylcyclopenten-l -yl)-5-(5-fluoro-2- methoxy-4-pyridyl)-3-thienyl]methoxy]phenyl]propanoic acid;

Cpd 28, (35)-3-cyclopropyl-3-[3-[[4-(5,5-dimethylcyclopenten-l-yl)-5 -(5-methoxy-3- pyridyl)-3-thienyl]methoxy]phenyl]propanoic acid;

Cpd 29, (35)-3-[3-[[2-chloro-5-(2-fluoro-5-methoxy-phenyl)-4-(2,2,2- trifluoro-l-methyl- ethyl)-3 -thienyl] methoxy] phenyl] -3 -cyclopropy 1-propanoic acid;

Cpd 30, (35)-3-cyclopropyl-3-[3-[[4"(5,5-dimethyicyclopenten-l -yl)-5-(2-fluoro-5- methoxy-phenyl)isothiazol-3-yl]methoxy]phenyl]propanoic acid;

Cpd 31, (35)-3-cyclopropyl-3-[3-[[4-(5,5-dimethylcyclopenten-l-yl)-5 -(2-methoxy-4- pyridyl)-3-thienyl]methoxy]phenyl]propanoic acid;

Cpd 32, (35)- 3 -cyclopropy 1-3 - [3 - [ [5 ~(2~f luoro-5 -methoxy-pheny l)-4-(5 -methyl-2- thienyl)isoxazol-3-yl]methoxy]phenyl]propanoic acid;

Cpd 33, (35)-3-cyclopropyl-3-[3-[[5-(2-fluoro-5-methoxy-phenyl)-4-(3 -methyl-2- thienyl)isoxazol-3-yl]methoxy]phenyl]propanoic acid;

Cpd 34, (3 _i S ^' )-3-cyclopropyl-3-[3-[[5-(2-fluoro-5-methoxy-phenyl)-4 -phenyl-isoxazol-3- yl]methoxy]phenyl]propanoic acid;

Cpd 35, (3 _i S ^' )-3-cyclopropyl-3-[3-[[5-(2-fluoro-5-methoxy-phenyl)-4 -isobutyl-isoxazol-3- yl]methoxy]phenyl]propanoic acid;

Cpd 36, (3S)-3 -cyclopropy 1-3 - [3 - [ [5 -(2-fluoro-5 -methoxy-ph eny l)-4-(2-methylprop- 1 - enyl)isoxazol-3-yl]methoxy]phenyl]propanoic acid;

Cpd 37, (3 )-3-cyclopropyl-3-[3-[[4-cyclopropyl-5-(2-fluoro-5-methoxy-p henyl)isoxazol 3 -y i ] methoxy ] phenyl] propanoic acid;

Cpd 38, (3 )-3-[3-[[5-(5-cyano-2-fluoro-phenyl)-4-(5,5-dimethylcyclopen ten-l -yl)-3- thienyl]methoxy]phenyl]-3-cyclopropyl-propanoic acid;

Cpd 39, (3 V3-cyclopropyl-3-f3-ff5-(2-fluoro-5-methoxy-phenyl)-4-isobut oxy-isoxazol- 3 -y i ] methoxy ] phenyl] propanoic acid; Cpd 40, (35)-3-[3-[[4-(cyclopentoxy)-5-(2-fluoro-5-methoxy-phenyl)is oxazol-3- yl]methoxy]phenyl]-3-cyclopropyl-propanoic acid;

Cpd 41, (35)-3-cyclopropyl-3-[3-[[4-(cyclopropylmethoxy)-5-(2-fluoro -5-methoxy- phenyl)isoxazol-3-yl]methoxy]phenyl]propanoic acid;

Cpd 42, (3^)-3-cyclopropyl-3-[3-[[5-(2-fluoro-5-methoxy-phenyl)-4-is opropoxy-isoxazol-

3 -yl]methoxy]phenyl]propanoic acid;

Cpd 43, (3^)-3-[3-[[4-(cyclobutoxy)-5-(2-fluoro-5-methoxy-phenyl)iso xazol-3- yl]methoxy]phenyl]-3-cyclopropyl-propanoic acid;

Cpd 45, (35)-3-[3-[[4-(5-tert-butyl-2-thienyl)-5-(2-fluoro-5-methoxy -phenyl)isoxazol-3- yl]methoxy]phenyl]-3-cyclopropyl-propanoic acid;

Cpd 46, (35)-3-[3-[[4-(cyclohexoxy)-5-(2-fluoro-5-methoxy-phenyl)iso xazol-3- yl]methoxy]phenyl]-3-cyclopropyl-propanoic acid;

Cpd 47, (3S)- 3 -cyclopropyl-3 - [3 - [ [5 ~(5~f luoro-2-methoxy-4-pyridyl)-4-isobutoxy - isoxazol-3-yl]methoxy]phenyl]propanoic acid;

Cpd 48, (35)-3-[3-[[4-(cyclohexoxy)-5-(5-fluoro-2-methoxy-4-pyridyl) isoxazol-3- yl]methoxy]phenyl]-3-cyclopropyl-propanoic acid;

Cpd 49, (3 _i S ^' )-3-cyclopropyl-3-[3-[[5-(2-fluoro-5-methoxy-phenyl)-4 -(3,3,3- trifluoropropoxy)isoxazol-3-yl]methoxy]phenyl]propanoic acid;

Cpd 50, (3 _i S ^' )-3-cyclopropyl-3-[3-[[4-ethoxy-5-(2-fluoro-5-methoxy- phenyl)isoxazol-3- yl]methoxy]phenyl]propanoic acid;

Cpd 51, (35)-3-cyclopropyl-3-[3-[[4-ethoxy-5-(5-fluoro-2-methoxy-4-p yridyl)isoxazol-3- yl]methoxy]phenyl]propanoic acid;

and

Cpd 52, (35)-3-cyclopropyl-3-[3-[[5-(5-fluoro-2-methoxy-4-pyridyl)-4 -(2-methylprop-l- enyl)isoxazol-3-yl]methoxy]phenyl]propanoic acid;

or a pharmaceutically acceptable salt form thereof. For use i medicine, salts of a compound of Formula (I) refer to non-toxic

"pharmaceutically acceptable salts." Other salts may, however, be useful in the preparation of a compound of Formula (I) or of their pharmaceutically acceptable salt forms thereof. Suitable pharmaceutically acceptable salts of a compound of Formula (I) include acid addition salts that can, for example, be formed by mixing a solution of the compound with a solution of a pharmaceutically acceptable acid such as, hydrochloric acid, sulfuric acid, fumaric acid, maleic acid, succinic acid, acetic acid, benzoic acid, citric acid, taitaric acid, carbonic acid or phosphoric acid. Furthermore, where the compound of Formula (I) carries an acidic moiety, suitable pharmaceutically acceptable salts thereof may include alkali metal salts such as, sodium or potassium salts; alkaline earth metal salts such as, calcium or magnesium salts; and salts formed with suitable organic ligands such as, quaternary ammonium salts. Thus, representative pharmaceutically acceptable salts include acetate, benzenesulfonate, benzoate, bicarbonate, bisulfate, bitartrate, borate, bromide, calcium edetate, camsylate, carbonate, chloride, clavulanate, citrate,

dihydrochloride, edetate, edisylate, estolate, esylate, fumarate, gluceptate, gluconate, glutamate, glycollylarsanilate, hexylresorcinate, hydrabamine, hydrobromide,

hydrochloride, hydroxynaphthoate, iodide, isothionate, lactate, lactobionate, laurate, malate, maleate, mandelate, mesylate, methylbromide, methylnitrate, methyls ulfate, mucate, napsylate, nitrate, N-methylglucamine ammonium salt, oleate, pamoate

(embonate), palnutate, pantothenate, phosphate/diphosphate, polygalacturonate, salicylate, stearate, sulfate, subacetate, succinate, tannate, tartrate, teoclate, tosylate, triethiodide, and valerate.

Representative acids and bases that may be used in the preparation of

pharmaceutically acceptable salts include acids including acetic acid, 2,2-dichloroacetic acid, acylated ammo acids, adipic acid, alginic acid, ascorbic acid, L-aspartic acid, benzenesulfomc acid, benzoic acid, 4-acetamidobenzoic acid, (-t-)-camphoric acid, camphorsulfonic acid, (+)-(! S)-camphor-10-sulfonic acid, capric acid, caproic acid, caprylic acid, cmnamic acid, citric acid, cyclamic acid, dodecylsulfuric acid, ethane- 1,2- disulfonic acid, ethanesuifonic acid, 2-hydroxy-ethanesulfonic acid, formic acid, fumaric acid, gaiactaric acid, gentisic acid, glucoheptonic acid, D-gluconic acid, D-glucoronic acid, L-glutamic acid, a-oxo-glutaric acid, glycolic acid, hippuric acid, hydrobromic acid, hydrochloric acid, (+)-L-lactic acid, (±)-DL-lactic acid, lactobionic acid, maleic acid, (-)- L-malic acid, malonic acid, (±)-DL-mandelic acid, methanesulfonic acid, naphthalene-2- sulfonic acid, naphthalene-l ,5-disulfonic acid, 1 -hydroxy-2-naphthoic acid, nicotinic acid, nitric acid, oleic acid, orotic acid, oxalic acid, palmitic acid, pamoic acid, phosphoric acid, L-pyroglutamic acid, salicylic acid, 4-amino-salicylic acid, sebaic acid, stearic acid, succinic acid, sulfuric acid, tannic acid, (+)-L-tartaric acid, thiocyanic acid, p- toluenesulfonic acid and undecylenic acid; and bases including ammonia, L-argimne, benethamine, benzathine, calcium hydroxide, choline, deanoi, diethanolamine,

diethylamine, 2-(diethylamino)-ethanol, ethanolamine, ethylenediamine, N-methyl- glucamine, hydrabamine, lH-imidazole, L-lysine, magnesium hydroxide, 4-(2- hydroxyethyl)-morpholine, piperazme, potassium hydroxide, 1 -(2-hydroxyethyl)- pyiTolidine, sodium hydroxide, triethanolamme, tromethamine, and zinc hydroxide.

Embodiments of the present invention include prodrugs of a compound of Formula (I). In general, such prodrugs will be functional derivatives of the compounds that are readily convertible in vivo into the required compound. Thus, in the methods of treating or preventing embodiments of the present invention, the term "administering" encompasses the treatment or prevention of the various diseases, conditions, syndromes and disorders described with the compound specifically disclosed or with a compound that may not be specifically disclosed, but which converts to the specified compound in vivo after administration to a patient. Conventional procedures for the selection and preparation of suitable prodrug derivatives are described, for example, in "Design of Prodrugs", ed. H. Bundgaard, Elsevier, 1985.

Where the compounds according to embodiments of this invention have at least one chiral center, they may accordingly exist as enantiomers. Where the compounds possess two or more chiral centers, they may additionally exist as diastereomers. it is to be understood that all such isomers and mixtures thereof are encompassed within the scope of the present invention. Furthermore, some of the crystalline forms for the compounds may exist as polymorphs and as such are intended to be included in the present invention. In addition, some of the compounds may form solvates with water (i.e., hydrates) or common organic solvents, and such solvates are also intended to be encompassed within the scope of this invention. The skilled artisan will understand that the term compound as used herein, is meant to include a solvated compound of Formula (I).

Where the processes for the preparation of the compounds according to certain embodiments of the invention give rise to mixture of stereoisomers, these isomers may be separated by conventional techniques such as, preparative chromatography. The compounds may be prepared in racemic form, or individual enantiomers may be prepared either by enantiospecific synthesis or by resolution. The compounds may, for example, be resolved into their component enantiomers by standard techniques such as, the formation of diastereomeric pairs by salt formation with an optically active acid such as,

(-)-di-p-toluoyl-d-tartaric acid and/or (+)-di-p-toluoyl-l-†artaric acid followed by fractional crystallization and regeneration of the free base. The compounds may also be resolved by formation of diastereomeric esters or amides, followed by chromatographic separation and removal of the chiral auxiliary. Alternatively, the compounds may be resolved using a chiral HPLC column.

One embodiment of the present invention is directed to a composition, including a pharmaceutical composition, comprising, consisting of, and/or consisting essentially of the (+)-enantiomer of a compound of Formula (I) wherein said composition is substantially free from the (-)-isomer of said compound. In the present context, substantially free means less than about 25 %, preferably less than about 10 %, more preferably less than about 5 %, even more preferably less than about 2 % and even more preferably less than about 1 % of the (-)-isomer calculated as

, . (mass (+) - enantiomer)

% ( + ) - enantiomer =

(mass (+) - enantiomer) + (mass(-) - enantiomer)

Another embodiment of the present invention is a composition, including a pharmaceutical composition, comprising, consisting of, and consisting essentially of the (-)-enantiomer of a compound of Formula (I) wherein said composition is substantially free from the (+)-isomer of said compound. In the present context, substantially free from means less than about 25 %, preferably less than about 10 %, more preferably less than about 5 %, even more preferably less than about 2 % and even more preferably less than about 1 % of the (+)-isomer calculated as

(mass (-) - enantiomer)

% (-) - enantiomer = x 100

(mass (+) - enantiomer ) + (mass(~) - enantiomer)

During any of the processes for preparation of the compounds of the various embodiments of the present invention, it may be necessary and/or desirable to protect sensitive or reactive groups on any of the molecules concerned. This may be achieved by means of conventional protecting groups such as those described in Protective Groups in Organic Chemistry, Second Edition, J.F.W. McOmie, Plenum Press, 1973; T.W. Greene & P.G.M, Wuts, Protective Groups in Organic Synthesis, John Wiley & Sons, 1991 ; and T.W. Greene & P.G.M. Wuts, Protective Groups in Organic Synthesis, Third Edition, John Wiley & Sons, 1999. The protecting groups may be removed at a convenient subsequent stage using methods known from the art.

Even though the compounds of embodiments of the present invention (including their pharmaceutically acceptable salts and pharmaceutically acceptable solvates) can be administered alone, they will generally be administered in admixture with a

pharmaceutically acceptable carrier, a pharmaceutically acceptable excipient and/ or a pharmaceutically acceptable diluent selected with regard to the intended route of administration and standard pharmaceutical or veterinary practice. Thus, particular embodiments of the present invention are directed to pharmaceutical and veterinary compositions comprising one or more compounds of Formula (I) and at least one pharmaceutically acceptable carrier, pharmaceutically acceptable excipient, and/or pharmaceutically acceptable diluent. By way of example, in the pharmaceutical compositions of embodiments of the present invention, the compounds of Formula (I) may be admixed with any suitable binder(s), lubrieant(s), suspending agent(s), coating agent(s), solubilizing agent(s), and combinations thereof.

Solid oral dosage forms, such as tablets or capsules, containing the compounds of the present invention may be administered in at least one dosage form at a time, as appropriate. It is also possible to administer the compounds in sustained release formulations.

Additional oral forms in which the present inventive compounds may be administered include elixirs, solutions, syrups, and suspensions; each optionally containing flavoring agents and coloring agents.

Alternatively, compounds of Formula (I) can be administered by inhalation (intratracheal or intranasal) or in the form of a suppository or pessary, or they may be applied topically in the form of a lotion, solution, cream, ointment or dusting powder. For example, they can be incorporated into a cream comprising, consisting of, and/or consisting essentially of an aqueous emulsion of polyethylene glycols or liquid paraffin. They can also be incorporated, at a concentration of between about 1 % and about 10 % by weight of the cream, into an ointment comprising, consisting of, and/or consisting essentially of a wax or soft paraffin base together with any stabilizers and preservatives as may be required. An alternative means of administration includes transdermal administration by using a skin or transdermal patch.

The pharmaceutical compositions of the present invention (as well as the compounds of the present invention alone) can also be injected parenterally, for example, mtracavernosally, intravenously, intramuscularly, subcutaneously, mtradermally, or intrathecally. In this case, the compositions will also include at least one of a suitable carrier, a suitable excipient, and a suitable diluent.

For parenteral administration, the pharmaceutical compositions of the present invention are best used in the form of a sterile aqueous solution that may contain other substances, for example, enough salts and monosaccharides to make the solution isotonic with blood.

For buccal or sublingual administration, the pharmaceutical compositions of the present invention may be administered in the form of tablets or lozenges, which can be formulated in a conventional manner.

By way of further example, pharmaceutical compositions containing at least one of the compounds of Formula (I) as the active ingredient can be prepared by mixing the compound(s) with a pharmaceutically acceptable carrier, a pharmaceutically acceptable diluent, and/or a pharmaceutically acceptable excipient according to conventional pharmaceutical compounding techniques. The carrier, excipient, and diluent may take a wide variety of forms depending upon the desired route of administration (e.g., oral, parenteral, etc.). Thus, for liquid oral preparations such as, suspensions, syrups, elixirs and solutions, suitable carriers, excipients and diluents include water, glycols, oils, alcohols, flavoring agents, preservatives, stabilizers, coloring agents and the like; for solid oral preparations such as, powders, capsules, and tablets, suitable carriers, excipients and diluents include starches, sugars, diluents, granulating agents, lubricants, binders, disintegrating agents and the like. Solid oral preparations also may be optionally coated with substances such as, sugars, or be enterically coated so as to modulate the major site of absorption and disintegration. For parenteral administration, the carrier, excipient and diluent will usually include sterile water, and other ingredients may be added to increase solubility and preservation of the composition, injectable suspensions or solutions may also be prepared utilizing aqueous carriers along with appropriate additives such as, solubilizers and preservatives.

A therapeutically effective amount of a compound of Formula (I) or a

pharmaceutical composition thereof includes a dose range from about 0.1 mg to about 3000 mg, or any particular amount or range therein, in particular from about 1 mg to about 1000 mg, or any particular amount or range therein, or, more particularly, from about 10 mg to about 500 mg, or any particular amount or range therein, of active ingredient in a regimen of about 1 to about 4 times per day for an average (70 kg) human; although, it is apparent to one skilled in the art that the therapeutically effective amount for a compound of Formula (I) will vary as will the diseases, syndromes, conditions, and disorders being treated.

For oral administration, a pharmaceutical composition is preferably provided in the form of tablets containing about 1.0, about 10, about 50, about 100, about 150, about 200, about 250, and about 500 milligrams of a compound of Formula (I).

Advantageously, a compound of Formula (I) may be administered in a single daily dose, or the total daily dosage may be administered in divided doses of two, three and four times daily.

Optimal dosages of a compound of Formula (I) to be administered may be readily determined and will vary with the particular compound used, the mode of administration, the strength of the preparation and the advancement of the disease, syndrome, condition or disorder. In addition, factors associated with the particular subject being treated, including subject gender, age, weight, diet and time of administration, will result in the need to adjust the dose to achieve an appropriate therapeutic level and desired therapeutic effect. The above dosages are thus exemplary of the average case. There can be, of course, individual instances wherein higher or lower dosage ranges are merited, and such are within the scope of this invention.

Compounds of Formula (I) may be administered in any of the foregoing

compositions and dosage regimens or by means of those compositions and dosage regimens established in the art whenever use of a compound of Formula (I) is required for a subject in need thereof.

As GPR40 receptor agonists, the compounds of Formula (I) are useful in methods for treating or preventing a disease, a syndrome, a condition or a disorder in a subject, including an animal, a mammal and a human in which the disease, the syndrome, the condition or the disorder is affected by the modulation, including agonism, of the GPR40 receptor. Such methods comprise, consist of and/or consist essentially of administering to a subject, including an animal, a mammal, and a human, in need of such treatment or prevention, a therapeutically effective amount of a compound, salt or solvate of Formula (I) ^. In another embodiment, the present invention is directed to a compound of Formula (I) for use in the treatment of a disorder affected by the agomsm of GPR40 receptor selected from the group consisting of obesity, obesity related disorders, impaired oral glucose tolerance, insulin resistance, Type II diabetes mellitus, metabolic syndrome, metabolic syndrome X, dvslipidemia, elevated LDL, elevated triglycerides, obesity induced inflammation, osteoporosis and obesity related cardiovascular disorders:

preferably, obesity, insulin resistance, Type II diabetes mellitus, dvslipidemia or metabolic syndrome X; more preferably, Type II diabetes mellitus or dvslipidemia.

GENERAL SYNTHETIC METHODS

Representative compounds of the present invention can be synthesized in accordance with the general synthetic methods described below and illustrated in the schemes and examples that follow. Since the schemes are an illustration, the invention should not be construed as being limited by the chemical reactions and conditions described in the schemes and examples. The various starting materials used in the schemes and examples are commercially available or may be prepared by methods well within the skill of persons versed in the art. The variables are as defined herein.

Abbreviations used in the instant specification, particularly the schemes and examples, are as follows:

ACN acetonitrile

AcOH glacial acetic acid

ADDP 1 , 1 '-(azodicarbony l)-dipiperidine

A1BN azobisisobutyroniiriie

aq. aqueous

B -.Pin . bis(pmaeolato)diboiOf!

Bn or Bzl benzyl

ΒΓΝΑΡ 2,2 ^! ~bis(dipheiiylphosphiiio)- 1 , Γ- liyl Boc tert-butyloxycarbonyl

cone. concentrated

dba dibenzylideneacetone

DBU l,8-diazabicyclo[5.4.0]undec-7-ene

DCE 1 ,2-dichloroethane

DCM dichloromethane

DEAD diethylazodicarboxylate

DIBALH or DIBAL diisobutylaluminum hydride

DIPEA or DIEA diisopropyl-ethyl amine

DMA dimethylaniline

DMAP 4-dimethy laniinopy ridin e

DME dimethoxyethane or dimethyl ether

DMF N,N-dimethylformamide

DMSO dimethylsulfoxide

DPPA diphenylphosphoryl azide

dppf 1 , 1 '-bis(diphenylphosphino)ferrocene dppp 1 ,3-bis(diphenylphosphino)propane

EA ethyl acetate

ESI electrospray ionization

EtOAc or EA ethyl acetate

EtOH ethanol

GCMS gas chromatography mass spectrometry h or hr(s) hour or hours

HPLC high performance liquid chromatography

IBX 2-iodoxy benzoic acid

LAH lithium aluminum hydride

LDA lithium diisopropylamide

LHMDS lithium bis(trimethylsilyl)amide

MEK methyl ethyl ketone MeOH methanol

MHz megahertz

mm minute or minutes

mass spectrometry

methanesulfonyl

NBS iV-bromosuccinimide

NMR nuclear magnetic resonance

PPTS pyridinium p-toluenesulfonate

RP reverse-phase

rt or RT room temperature

Rt retention time

RuPhos bis(2',6'-diisopropoxybiphenyl)

cyclohexylphosphine

sec second or seconds

SPhos 2-dicyclohexylphospWno-2',6'-dimethoxybiphenyl

TBAF tetrabutylammonium fluoride

TBDMS t-but ldimethylsilyl

TBP trihutyiphosphate

TEA or Et ₃ I triethylamine

TFA trifluoroacetic acid

THF tetrahydrofuran

TLC thin layer chromatography

IMS tetramethylsilane

Tf trifluoromethanesulfonyl

Ts 4-toluenesul f ony i

XPhos 2-dicyclohexylphosphino-2', 4', 6'- triisopropyibiphenyl

General Schemes Compounds of formula (I) may be prepared according to the process as described in the Scheme A. below.

heme A

x>2

R ⁵ CH=CHC0 ₂ R ^{A R "M} _» R ⁵ CH-CH ₂ C0 ₂ R

A2

A compound of formula A2 may be prepared as shown in Scheme A. An acrylic ester of formula Al, wherein R ⁵ is 3-hydroxyphenyl, may be used as a substrate for a conjugate addition reaction with a compound of formula R ² -M, wherein M is a metal, to obtain a compound of formula A2. The starting acrylate of formula Al is either commercially available or may be prepared according to the methods described in the scientific literature. A compound of formula R ² -M may be (a) a boronic acid to form a compound of formula R ² -B(OH)2; (b) a suitably selected boronic ester such as

pinacolatoboryl, neopentylglycolatoboryl, and the like; (c) a suitably selected copper reagent or (d) a suitably selected Grignard reagent. One of ordinary skill in the art will understand that a catalyst, optionally in the presence of a ligand, may be required with the use of certain available reagents. In addition, with the use of certain other

organometallic reagents, such as a Grignard reagent or a cuprate, the free hydroxyl group may need to be protected with an appropriate hydroxyl protecting group, which may be removed at a later stage in the synthetic sequence. A preferred method for this transformation includes treatment of a compound of formula Al with a compound of R -M wherein M is a boronic acid; in the presence of an R_h catalyst; with a suitable ligand such as BINAP. When optically pure BINAP is employed, an enantiomerically enriched compound of formula A2 may be prepared. A compound of formula A2 may be subjected to chiral separation to obtain an optically pure enantiomer. When a compound of formula A3 is employed in place of the aery late starting material of formula Al, it is necessary to esterify the resultant carboxylic acid of formula A4 to obtain the desired compound of formula A2. One of ordinary skill in the art will recognize that in certain instances, substituent R ³ may be interchanged with substituent R ² . Scheme B illustrates a method for the preparation of certain compounds of formula

(la) of the present invention wherein X is O, Y is N.

Scheme J8

B6 B7 B8 (la)

The ring closure of an R'-substituted dioxobutanoate of formula B2 with NH ₂ OH is the key step in the construction of the desired isoxazole core structure of the present invention. A solution of a compound of formula B2 may be stirred with a source of NH ₂ OH such as its hydrochlonde salt; in a suitable solvent such as EtOH; under reflux conditions; to effect the cyclo-condensation reaction to obtain a compound of formula B3. The required dioxobutanoate of formula B2 may be obtained by the alkylation of the enolate derived from a ketone of a compound of formula Bl with a reagent such as diethyl oxalate and the like. For example, a ketone of formula Bl may be treated with a base such as NaH, LDA, LiHMDS, LiTMP, or the like; at a temperature ranging from about -78 °C to about room temperature; in suitable solvent such as THF, Et ₂ 0, or DME; to generate the corresponding metal-enoiate which may then be reacted with diethyl oxalate. The method of choice for this transformation involves treatment of a ketone of formula B2 with NaH; in DMF solvent; at about room temperature, followed by interception of the resulting sodium enoiate with diethyl oxalate. For ketones of formula Bl that are not commercially available, one of ordinary skill in the art may use conventional, known methods described in the scientific literature. The ester group of a compound of formula B3 may be reduced to its corresponding primary alcohol with a suitable reducing agent such as LAH, DIB ALH, B ₂ ¾, and the like; in a suitable solvent such as DCM, DCE, THF or diethyl ether; at a temperature ranging from about -78 °C to about 50 °C; to obtain a compound of formula B4. A preferred method for this reduction includes the treatment of a compound of formula B3 with a reducing agent such as DIBALH; in THF; at a temperature of about -78 °C. The compound of formula B4 may then be halogenated to obtain a compound of formula B5 wherein X ¹ is a halogen, preferably bromo. A preferred method for carrying out this transformation includes, but is not limited to, the bromination of a compound of formula B4 with a brominating agent such as NBS or the like; in a solvent such as DMF or the like; at a temperature ranging from about room temperature to about 60 °C. The compound of formula B7 may be obtained from the coupling of a compound of formula B5 with a compound of formula A2. This transformation may be achieved by first converting the hydroxymethyl group of a compound of formula B5 to a suitable leaving group (L ) such as a halide, tosylate, mesylate, and the like, to obtain a compound of formula B6, followed by reaction with a compound of formula A2. A preferred method for this transformation includes the reaction of a compound of formula B5 with a halogenating agent such as SOCl? or the like; in a solvent such as DCM or DCE; to obtain a compound of formula B6 wherein L ^s is chloro. A compound of formula B6 may then be reacted with a compound of formula A2 in the presence of a suitable base such as Na ₂ C0 ₃ , K ₂ C0 ₃ or Cs ₂ C0 ₃ ; in a suitable solvent such as THF, DMF or DMSO; at a suitable temperature. Alternatively, a compound of formula B6 may be directly coupled with a compound of formula A2 under Mitsunobu reaction conditions to obtain a compound of formula B7. A preferred coupling method may includes, but is not limited to, the treatment of a mixture of a compound of formula B6 and a compound of formula A2 with a phosphine source such as PPI1 ₃ or the like; in the presence of a coupling agent such as DEAD or the like; in a suitable solvent such as THF, DCM, or the like; at a suitable temperature ranging from about 0 °C to about room temperature.

A compound of formula B7 may be reacted with a suitably substituted

compound of formula G-M ¹ , wherein G is a suitably selected group, under suitable coupling conditions, to yield the corresponding compound of formula B8. A compound of formula G-M ¹ may be (a) a boronic acid to form a compound of formula G-B(OH)2; (b) a suitably selected boronic ester such as pmaeolatoboryl, neopentylglycolatoboryl, and the like; (c) a suitably selected trialkylstannyl such as tri(w-butyl)tin, and the like; (d) a suitably selected trialkylsilyl such as triallylsilyl, and the like; or (e) a suitably selected aryldialkylsilyl such as 2-(hydroxymethyl)phenyl-dimethylsilyl, and the like; or (f) a suitably selected organo zinc reagent such as G-ZeX wherein X is a halide such as chloro, bromo, or iodo.

For example, a compound of formula G-M ¹ , wherein M ¹ is preferably -B(OH) ₂ or a boronic ester, may be reacted with a compound of formula B8 under Suzuki coupling conditions, more particularly in the presence of a suitably selected palladium catalyst such as palladium (IT) acetate, palladium (II) chloride, &«"(acetomtrile)- dichloro-paliadium(II), allylpalladium (II) chloride dirner, tm(dibenzylidineacetone) dipalladium (0) f Fdndbii )-. ). 2-(di-tert-butylphosphino)biphenyl,

buty lphenylphosphine)-palladium (II), [ 1 , 1 ' -i»/5 ^, -(diphenylphosphino)-ferrocene] - palladium (II) dichloride dichloromethane adduct ((dppf)PdCl ₂ .DCM),

reiraAis(triphenylphosphine) palladium(O) (Ρά(ΡΡη ₃ ) ₄ ), (l ,V-bis(di-tert- butylphosphmo)ferrocene palladium (II) chloride, and the like; optionally in the presence of a suitably selected ligand such as triphenylphosphine, tri-otolylphosphine, tri(t<?rt-butyl)-phosphine, tricy ciohexylphosphine, 1 , 1 '-bis(diphenylphosphino)- ferrocene, 2-(dicyclohexylphosphino)-2',4',6'-tri-i-propyl-l, l '-biphenyl, S-Phos, Ru- Phos, to[2-(diphenyl-phosphino)phenyl] ether, 2-dicyclohexylphosphino-2',6'- dimethoxybiphenyl, tris(2-furyl)phosphine, 1 -butyl-3-methylimidazolium

hexafluorophosphate, and the like; in the presence of a suitably selected inorganic base such as cesium carbonate, potassium carbonate, sodium carbonate, cesium fluoride, potassium fluoride, tetrabutylammonium fluoride, potassium feri-butoxide, sodium tert- butoxide, sodium hydroxide, sodium bicarbonate, potassium phosphate, or preferably sodium carbonate; in a suitably selected solvent such as ethanol, THF, DMF, toluene, benzene, DME, H ₂ 0, 1 ,4-dioxane, and the like, or a combination thereof; at a temperature in the range of from about room temperature to about 180 °C.

In the final step, the ester functionality of a compound of formula B8 may undergo a conventional saponification to obtain a compound of formula (la). One of ordinary skill in the art will recognize that there are a variety of reagents and reaction conditions available for this conversion. A preferred method for this transformation includes treatment of a compound of formula B7 with an aqueous base such as NaOH, LiOH, and the like; in a mixed solvent such as THF/MeOH, and the like; at about room temperature.

It is also understood that it is possible to alter the sequence of some of the synthetic transformations described above without affecting the final outcome of the synthesis.

Scheme C illustrates a method for the preparation of certain compounds of Formula (Ila) of the present invention where in XB is O, YB is N.

Scheme C

(Ila)

The construction of the desired isoxazole structure may be achieved by a 1 ,3- dipolar cycloaddition reaction of an in situ generated nitrile oxide (derived from the chloro oxime of a compound of formula C3) and a propiolate dipolarophile C5. The aldehyde of a formula CI wherein R ^1B is a suitably substituted aryl or a heteroaryl ring, may be first converted to its corresponding aldoxime C2 under standard conditions, which may then be converted to a chloro oxime of formula C3 by treatment with a chlorinating agent such as NCS, at about 0 °C to about room temperature. The chloro oxime of formula C3 may be treated with a base such as EtsN in a solvent such as DCM; in the presence of a dipolarophile such as ethyl propiolate, C5; to obtain a cycloaddition product of formula C4. One of ordinary skill in the art will recognize that there are alternative synthetic methods available for the preparation of an isoxazole of formula C4. A compound of formula C4 may be converted to a compound of formula (Ila) following the methodologies described in Scheme B for the synthesis of a compound of formula (I) via a compound of formula B3.

Scheme D illustrates a method for the preparation of certain compounds of formula (Ilia) of the present invention wherein Y _c is CH, Z _c is N.

Scheme D

The cyanoglyoxylate oxime of formula Dl may be reduced to an amino- cyanoacetate of formula D2 using one of a variety of available reagents and conditions, for example: (1) H ₂ /Pd, H ₂ /Pt, or Al amalgam in an alcoholic solvent such as MeOH or EtOH; and (2) Na ₂ S ₂ 0 ₄ or Na ₂ S ₂ 0 ₃ as a reducing agent. A preferred method for this transformation includes treatment of a compound of formula Dl with Na ₂ S ₂ 0 ₄ ; in aqueous NaHC0 ₃ ; at about room temperature. The amino-cyanoacetate of formula D2 may then be reacted with an orthoester, such as ethyl orthoformate, to generate an intermediate such as D3 in-situ. This intermediate may react with an aniline of formula R ^1C -NH ₂ to obtain a compound of formula D4. Alternati vely, the aniline of formula R ^1C -NH ₂ may be converted to an N-aryl formimidate of formula R ^l N=CHOEt, for example, which may then be reacted with a compound of formula D2 to obtain a compound of formula D4.

A compound of formula D4 may be converted to a compound of formula D5 wherein X ² is a halogen. A preferred method for this transformation includes, but is not limited to, the non-aqueous diazotization of the amino group of a compound of formula D4 using a reagent such as tert-but l nitrite or isoaniyl nitrite, followed by the interception of the resulting diazonium salt with an appropriate halogen source such as I ₂ / CH ₂ I ₂ , Cu ₂ Br ₂ , Cu ₂ Cl ₂ , and the like, to give a compound of formula D5 wherein X ² is iodo, bromo or chloro respectively. It is also understood that this transformation may be effected using standard aqueous Sandmeyer conditions.

A compound of formula 1 ⁾ 5 may be reacted with a suitably substituted compound of formula Gc-M ¹ , wherein Gc is a suitably selected group, under suitable coupling conditions, to yield the corresponding compound of formula D6 using the methods described for the preparation of a compound of formula B8 from a compound of formula J87 (Scheme B).

The ester group of a compound of formula 1 ⁾ 6 may then be reduced to its corresponding hydroxymethyl group to obtain a compound of formula D7. One of ordinary skill in the art will recognize that there are a variety of reagents and reaction conditions available for this transformation. A preferred set of reaction conditions for this conversion includes treatment of a compound of formula D6 in a suitable solvent such as diethyl ether, THF or DCM; with a hydride source such as LAH or DIBAL-H, or the like; at a temperature at about -78 °C to about room temperature.

The alcohol of formula D7 may be coupled with a compound of formula A2 under standard Mitsunobu conditions to obtain a compound of formula D9. A preferred method for this transformation includes, but is not limited to, the coupling of a

compound of formula D7 with a compound of formula A2; using PPh ₃ and a coupling agent such as DEAD; in a suitable aprotic solvent such as THF; at a suitable temperature ranging from about 0 °C to about room temperature. Alternatively, the hydroxymethyl functionality of a compound of formula D7 may be converted to a leaving group such as a chloride to obtain a compound of formula D8, which may then be reacted with a compound of formula A2 in the presence of a suitable base such as Na ₂ C0 ₃ , K ₂ C0 ₃ , or Cs ₂ C0 ₃ ; in a suitable solvent such as THF, DMF or DMSO; at a suitable temperature. A preferred method for this transformation includes, but is not limited to, treatment of a compound of formula D7 with SOCl ₂ ; in DCM solvent; at room temperature; to obtain the corresponding benzy l chloride of formula D8. The compound of formula D8 may then be reacted with a compound of formula A2 in the presence of Cs ₂ C0 ₃ as a base; in a solvent such as DMF; at room temperature; to yield a compound of formula D9.

In the final step, the ester functionality of a compound of formula D9 may undergo a conventional saponification to obtain a compound of formula (Ilia). One of ordinary skill in the art will recognize that there are a variety of reagents and reaction conditions available for this conversion. A preferred method for this transformation includes treatment of a compound of formula D9 with an aqueous base such as NaOH, LiOH, and the like; in a mixed solvent such as THF MeOH, and the like; at about room temperature.

Scheme E illustrates a method for the preparation of certain compounds of formula (l f the present invention where in X is S, Y is N.

E1 E2 E3 E5 (lb)

The key step in this synthetic sequence is the 1,3-dipoiar cycloaddition of m-situ generated nitrilesulfide of formula E3 with an appropriate dipolarophiie of formula E4 to obtain a compound of formula E5. The amide of formula El is either commercially available or can be prepared according to the methods described in the scientific literature. The amide El can be reacted with ClCOSCl in an inert solvent such as THF, DCE , benzene, toluene, dioxane and the like at about 50-200 °C to obtain a compound of formula E2. The preferred method for this transformation includes the reaction of the amide El with ClCOSCl in toluene at 100 °C for about 12 h. The nitrilesulfide intermediate E3 can be generated and trapped in-situ with a dipolarophiie by either the thermal or microwave assisted decarboxylation of the l,3,4-oxathiazol-2-one E2 in the presence of a compound of formula F4. The preferred method for this transformation includes the heating of a mixture of a compound of formula E2 and E4 where in ^E is a alkyl group in an inert solvent such as THF, DCE, benzene, toluene, dioxane and the like, preferably 1,3- dichlorobenzene at about 150 °C for about 5 h. Compound E5 can be converted to a compound of formula (lb) following the methodologies described in Scheme B for the synthesis of compounds of formula (la) from a compound of formula B3,

Scheme F illustrates a method for the preparation of certain compounds of formula (Illb) of the present invention where in Yc and Zc are each N and Lc is an ether.

The key step in this synthetic sequence is a 1,3 -dipolar cycloaddition of an azide R ^' -N ₃ of (Fl) with an appropriate β-ketoester of formula F2, in the presence of an organic base, to obtain a compound of formula F3, The azide of formula Fl and β-ketoester of formula F2 are either commercially available or may be prepared according to the methods described in the scientific literature. For instance, a compound of formula Fl may be reacted with a compound of formula F2 wherein Gc is Ci-eaikyl, or preferably isopropyl, and R ⁶ is ethyl; in a polar solvent such as DMSO; in the presence of an organic base such as Et ₂ NH, Et ₃ N, pyrrolidine, and the like; at about room temperature to about 150 °C, preferably at 80 °C; to obtain a compound of formula F3 wherein Gc is Cj.ealkyl, or preferably isopropyl, and R ⁶ is ethyl. The ester group of a compound of formula F3 may then be reduced to its corresponding alcohol as described hereinbelow. The alcohol may be converted to a compound of formula (Illb) either by the direct coupling with a compound of formula A2 or via a compound of formula F5 wherein L ² is a leaving group, preferably chloro, using the methods as previously described in Scheme B. The resulting product may be saponified to obtain a compound of formula (Hlb).

An alternate route for the preparation of a compound of formula (Illb) is shown in Route B. This route utilizes the cy cloaddition of an azide of formula Fl with a nitrile of formula F6, such as ethylcyanoacetate, to yield a compound of formula F7. The compound of formula F7 may be reduced with suitable reducing agent such as DIBAL-H; in a suitable solvent such as DCM, diethyl ether, THF, and the like; to obtain a compound of formula F8. The ammo functionality of a compound of formula F8 may be converted to a suitable functional group such as a halide, preferably chloro, bromo, or iodo, for the introduction of substituent Gc. For example, a compound of formula F8 may be diazotized with an appropriate reagent such as fert-BuN0 ₂ ; in the presence of a reagent such as CuBr ₂ ; to obtain a compound of formula F9 wherein X ³ is bromo. It is understood that these two steps may be reversed without affecting the final outcome of the two steps. As previously described, the compound of formula F9 may be carried through the synthesis to yield a compound of formula (Illb) using an adaptation to the methods described in Scheme B for the conversion of a compound of formula B5 to B7 to a compound of formula (la). Alternatively, a compound of formula F9 may be converted to a compound of formula Fll and earned through to furnish a compound of formula (Hlb), as described in the transformation of a compound of formula B5 to a compound of formula (la), with or without the protection of the hydroxyl function of the compound of formula F9.

Scheme G illustrates another method for the preparation of certain compounds of form la (lb) of the present invention where in X is S and Y is N.

Accordingly, a suitably substituted amide compound of formula Gl, a commercially available compound or compound prepared from a corresponding acid through known methods, may be reacted with a commercially available reagent of formula G2, in an organic solvent such as toluene, xylene, chlorobenzene and the like, at a temperature in the range from 100°C to about 160°C, to yield the corresponding compound of formula G3.

The compound of formula G3 may be reacted with a suitably substituted compound of formula G4, a commercially available compound or compound prepared by known methods, in an organic solvent such as toluene, chlorobenzene, 1,3-dichlorobenzene, and the like, at a temperature in the range from 150°C to about 200°C, to yield the

corresponding compound of formula G5.

The compound of formula GS may be reacted with a commercially available reducing agent such as LAH, DIBAL, borane/THF complex and the like, in an organic solvent such as THF, ether, dioxane and the like, at a temperature in the range from about -20 °C to about room temperature, to yield the corresponding compound of formula G6 wherein B is hydrogen.

The compound of formula G6 wherein B is hydrogen may be reacted with a suitably substituted compound of formula G7, a compound prepared by known methods, with a commercially available coupling agent such as DEAD/PI1 ₃ P, ADDP/TBP, and the like, in an organic solvent such as toluene, THF, dioxane and the like, at a temperature in the range from 0 °C to about 80 °C, to yield the corresponding compound of formula G8.

Alternatively, the compound of formula G6, wherein B is hydrogen, may be reacted with a commercially available reagent such as TsCl, MsCl, CCI ₄ /PI1 ₃ P, NBS/Pt P and the like, either in the presence or in the absence of an organic base such as TEA, DIPEA and the like, in an organic solvent such as ΤΉΕ, DCM, ether, and the like, at a temperature in the range of from 0 °C to room temperature, to yield the corresponding compound of formula G6, wherein -OB is a suitable leaving group such as OTs, OMs, CI, Br, and the like, which is then reacted with a suitably substituted compound of formula G7, a compound prepared by known methods, in the presence of an inorganic base such as CS2CO3, K2CO3, NaH and the like, in an organic solvent such as THF, acetone, DMF and the like, at a temperature in the range from room temperature to 100°C, to yield the corresponding compound of formula G8.

The compound of formula G8 may be reacted with a commercially available inorganic base such as LiOH, NaOH, KOFI and the like in a mixed solvent of THF, MeOH and water and the like, at a temperature in the range from 0°C to about 50°C, to yield the corresponding compound of formula (lb).

Compounds of formula (Ic) wherein X is S and Y is C( ') may alternatively be prepared according to the process outlined in Scheme H, below.

Scheme H

Accordingly, a commercially available or a compound prepared by known methods of formula HI may be reacted with a commercially available trifluoromethanesulfonic anhydride, in the presence of an organic base such as pyridine, TEA, DIPEA and the like, in an organic solvent such as DCM, 1,2-diehloroethane, THF and the like, at a temperature in the range of from 0 °C to room temperature, to yield the corresponding compound of formula H2.

The suitably substituted compound of formula H2 may be reacted with a suitably substituted compound of formula H3, a commercially available compound or compound prepared by known methods, in the presence of an inorganic base such as Na ₂ C0 ₃ , K ₂ C0 ₃ , K ₃ PO ₄ , and the like, in the presence of a suitably selected an Pd containing reagent such Pd(OAc) ₂ , Pd(Ph ₃ P) ₄ , Pd(Ph ₃ P) ₂ Cl ₂ , and the like, in the presence of a suitably selected ligand such as Ph ₃ P, BINAP, dppf, and the like, in a mixture of a suitably selected organic solvent such as toluene, ethanol, ,4-dioxane, and the like, and water, to yield the corresponding compound of formula H3.

The suitably substituted compound of formula H3 may be reacted an inorganic reducing reagent such as borane, LAH, DIBAL and the like, in an organic solvent such as THF, dioxane, ether and the like, at a temperature in the range from -20°C to room temperature, to yield the corresponding compound of formula H4 wherein B is hydrogen. The compound of formula H4 wherem B is hydrogen may be reacted with a suitably substituted compound of formula G7, a compound prepared by known methods, with a commercially available coupling agents such as DEAD/PI1 ₃ P, ADDP/TBP and the like, in an organic solvent such as toluene, THF, dioxane and the like, at a temperature in the range from 0 °C to about 80 °C, to yield the corresponding compound of formula 15.

Alternatively, the compound of formula H4, wherein B is hydrogen, may be reacted with a commercially available reagent such as TsCl, MsCl, CCI ₄ /PI1 ₃ P, NBS/Pt P and the like, either in the presence or in the absence of an organic base such as TEA, DIPEA and the like, in an organic solvent such as THF, DCM, diethyl ether and the like, at a temperature in the range from 0 °C to room temperature, to yield the corresponding compound of formula H4, wherein OB is a suitably leaving group such as OTs, OMs or CI, Br, and the like, which is then reacted with a suitably substituted compound of formula G7, a compound prepared by known methods, in the presence of an inorganic base such as CS ₂ CO ₃ , K ₂ CO ₃ , NaH and the like, in an organic solvent such as THF, acetone, DMF and the like, at a temperature in the range from room temperature to 100°C, to yield the corresponding compound of formula H5.

The compound of formula H5 may be reacted with a commercially available inorganic base such as Li OH, NaOH, KOH and the like in a mixed solvent of THF, MeOH and water and the like, at a temperature in the range from 0°C to about 50°C, to yield the corresponding compound of formula (Ic).

Compounds of Formula (I), (II) or (III) wherein L, L _b , or L _c , respectively, are either -CH=CH- or -(CH ₂ ) ₂ - , may be prepared according to the process outlined in Scheme I, below.

(I), (II) or (III) (I), (II) or (III) A compound of formula II where in Cy represents one of the 5 -member ed heterocyles described in schemes B-H, can be converted to the compound of formula 12 where in X is a halide, preferably Br. A preferred synthetic method for this conversion includes, but is not limited to, the treatment of the compound II with PBr-, in DCM at about 25 °C for 2 h. The halide 12 can then be converted to the Wittig reagent 13 by known methods. The Wittig reagent of formula 13 can be employed in an olefination reaction with the aldehyde of formula 16 using standard Wittig olefination protocol. For example, a compound of formula 13 may be reacted with 16 in the presence of a suitable base such as NaH, in a suitable solvent such as THF, at a suitable temperature in the range of from about 0 °C to 30 °C. It is understood that there are many other ways to convert the compound of formula 12 to 14. For example the compound 12 can be converted to Horner-Emmons reagent which can then be employed in an olefination reaction with the aldehyde 16 to obtain the compound of formula 14. Compound 16 may be prepared from compound A2. The preferred method of this transformation includes the conversion of the phenolic function of the A2 to corresponding inflate followed by the Pd catalyzed hydroformylation reaction with high pressure CO gas, preferably around 5 arm, under reductive conditions in the presence of an organic base such as triethyl amine and a reducing agent such as triethylsilane in a solvent such as DMF at a about 80 °C. The ethenyl functionality in compounds of formula 14 may be reduced using an appropriate reducing agent, such as NiCl ₂ / NaBI-I ₄ or H ₂ Pd/C, in a solvent such as ethyl acetate, MeOH, EtOH, to obtain a compound of formula 15. Preferred reaction conditions for this transformation include the hydrogenation of the compound of formula 14 in MeOH at room temperature over 10% Pd/ ^' C at about 3 atm of H ₂ pressure. In the final step, the ester functionality of a compound of formula 14 or 15 may undergo a conventional saponification to obtain a compound of formula (I), (II) or (III). One of ordinary skill in the art will recognize that there are a variety' of reagents and reaction conditions available for this conversion. A preferred method for this transformation includes treatment of a compound of formula 14 or 15 with an aqueous base such as NaOH, LiOH, and the like; in a mixed solvent such as

THF/MeOH, and the like; at about room temperature.

Specific Examples

Example 1

( ₁ S)-3-Cyclopropyl-3-(3-((5-(5,5-dimethylcyclopent-l-en- l-yl)-l-(2-fluoro-5- methoxyphenyl)-lH-pyrazol-4-yl)methoxy)phenyl)propanoic acid (Cpd 66)

To a mixture of 2-fluoro-5-methoxyphenylboronic acid (2.00 g, 11.8 mmol) and di-t-butyl azodicarboxylate (2.85 g, 12.4 mmol) in MeOH was added Cu(OAc) ₂ (214 mg, 1.18 mmol ) under argon. The mixture was stirred at 45 °C for 6 h and concentrated. The residue obtained was dissolved in DCM (100 mL) and washed with water, satd. NaHC0 ₃ , and brine. The organic layer was dried over Na ₂ S0 ₄ , filtered and concentrated. The crude residue was purified by column chromatography on silica gel (0-30% EtO Ac/heptane). The resulting yellow oil was dissolved in 1,4-dioxane (5 mL) and treated with 4M HC1 in 1,4-dioxane (18 mL, 64 mmol). The resulting mixture was stirred at 50 °C for 2 h, and at RT for 4 h. The precipitate formed was collected by filtration, washed with 1,4-dioxane and ether, and dried under high vacuum to obtain compound la as a white solid. 1H-NMR (400 MHz, OMSO-d ₆ ) δ (ppm): 10.21 (br. s., 2H), 8.28 (br. s., 1H), 7.13 (dd, J = 11.1, 8.6 Hz, 1H), 6.77 (dd, J= 7.1, 3.0 Hz, 1H), 6.50 (dt, ./ 9.0, 3.1 Hz, IH), 3.73 (s, 3H).

Ethyl 5-amino-l-(2-fluoro- -methoxyphenyl)-lH-pyrazole-4-carboxylate, lb

To a stirring solution of compound la (1.54 g, 8.00 mmol) and triethylamine (1.11 mL, 8.00 mmol) in EtOH (15 mL) was added ethyl (ethoxymethylene)cyanoacetate (1.49 g, 8.79 mmol) portion wise. The reaction was stirred at rt for 16 h. Water (100 mL) was added, and the mixture was extracted with EtO Ac (2 x 100 mL). The combined organic layers were washed with brine, dried over Na ₂ S0 ₄ , filtered and concentrated. The crude material was purified by column chromatography on silica gel (0-50% EtO Ac/heptane). The resulting solid was suspended in AcOH (5 mL) and water (1.6 mL) and stirred at 100 °C for 1 h. The reaction mixture was allowed to cool to RT and poured over ice. The resulting precipitate was extracted with EtO Ac (2 x 100 mL). The combined organic layers were washed with brine, dried over Na ₂ S0 ₄ , filtered and concentrated to give compound lb as a brown oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for C ₁₃ H ₁₄ FN ₃ 0 ₃ : 280.1 (M+H); found: 280.1, C. Ethyl 5-bromo-l-(2-fluoro-5-methoxyphenyl)-lH-pyrazole-4-carboxyla te, lc

To a solution of compound lb (133 mg, 0.476 mmol) and CuBr ₂ (128 mg, 0.571 mmol) in acetonitriie (2 mL), cooled in an ice-water bath, was added t- butyl nitrite (68.0 mL, 0.571 mmol) dropwise under argon. The reaction mixture was warmed to rt over 2 h, and stirred at rt overnight. EtOAc (50 mL) was then added and the organic layer was washed with water, IN HC1, and brine. After drying over Na ₂ S0 ₄ , the organic layer was filtered and concentrated. The resulting crude material was purified by flash chromatography (0-20 % EtO Ac/heptane). Compound lc was obtained as a white solid. Mass Spectrum (LCMS, ESI pos.): Calcd. for CnHnBrFNaOs: 343.0 (M+H); found: 343.0.

D. Ethyl 5-(5,5-dimethylcyclopent-l-en-l-yl)-l-(2-fluoro-5-methoxyphe nyl)-lH- pyrazoie-4-carboxyiate, I

A mixture of compound lc (104 mg, 0.303 mmol), 2-(5,5-dimethylcyclopent-l-en-l-yl)- 4,4,5, 5-tetramethyl-l,3,2-dioxaborolane (101 mg, 0.455 mmol) and K ₃ P0 ₄ (257 mg, 1.21 mmol) in 1,4-dioxane (2 mL) and water (0.5 mL) was purged with argon.

Dichloro(diphenylphosphinoferrocene)palladium (25 mg, 0.034 mmol) was then added and the mixture was stirred at 80 °C for 24 h. Upon cooling, EtO Ac (50 mL) was added. The organic layer was washed with water (50 mL) and brine (50 mL), dried over Na ₂ S0 ₄ , filtered and concentrated. The resulting crude material was purified by flash

chromatography (0-20 % EtO Ac/heptane). Compound Id was obtained as a white solid.

Mass Spectrum (LCMS, ESI pos.): Calcd. for ( :,! ! .. -I- VO ;: 359.2 (M+H); found: 359.2. E. (5-(5, 5-Dimethylcy clopent- 1 -en- 1 -y 1)- 1 -f 2-fluoro-5-methoxypheny 1)- lH-pyrazol- 4-yl)methanol, le

A solution of compound Id (91 .0 mg, 0.254 mmol) in toluene (4 mL) was cooled in a dry ice-acetone bath (-78 °C). DIBAL-H (1 M solution in toluene, 1 .52 mL, 1.52 rnmol) was added dropwise while under argon. The mixture was warmed to rt and stirred for 30 mm. The mixture was cooled to -78 °C again, and disodium tartrate (1M, 20 ml.) solution was added slowly to quench the reaction. EtOAc (20 mL) was added, and the mixture was stirred at rt for 1 h until the layers were separated. The organic layer was washed with brine, dried over Na ₂ S0 ₄ , filtered, and concentrated to obtain Compound le as a colorless oil, which was used in the next step without further purification. Mass Spectrum (LCMS, ESI pos.): Calcd. for ( ^' _: , _; Η , ; ί· ^' \ .()·■: 317.2 (M+H); found: 317.2.

F. (S)-Ethyl 3-cyclopropyl-3-(3-hydroxyphenyl)propanoate, If-S

a. Ethyl 3-cyclopropylacrylate, lf-A

To a solution of cyclopropanecarbaldehyde (20.4 g, 285 mmol) in THF (230 mL) at 0 °C under argon was added ethyl 2-(triphenylphosphoranylidene)acetate (107 g, 292 mmol) portion wise. The mixture was stirred at it for 16 h before removal of the solvent. The crude solid material obtained was suspended in heptane, sonicated and filtered. The filtrate was concentrated and purified by distillation under reduced pressure to afford compound Ig as a colorless oil. 1H-NMR (400 MHz, CDCI ₃ ) δ (ppm): 6.42 (dd, J= 15.7, 10.1 Hz, I I I ). 5.89 (d, ./ = 15.7 Hz, IH), 4.17 (q, J ------- 7, 1 Hz, 2H), 1 .51 - 1.67 (m, 1 1 1). 1.29 (t, ./ 7.1 Hz, 3H), 0,90 - 1.02 (m, 2H), 0.59 - 0.72 (m, 2H). b. Ethyl 3-cyclopropyl- -(3-hydroxyphenyl)propanoate, lf-B

To a mixture of chloro(l,5-cyclooctadiene)rhodium (I) dimer (1.79 g, 3.63 mmol) in 1,4- dioxane (50 mL) under argon was added 1 N aq. NaOH (109 mL, 109 mmol), (3- hydroxyphenyl)boronic acid (20.0 g, 145 mmol) and a solution of compound lf-A (10.2 g, 72.5 mmol) in 1,4-dioxane (50 mL) at it . The mixture obtained was stirred at 50 °C for 16 h. The reaction mixture was allowed to cool to RT, then poured into 1 : 1

EtQAc/i¾0 ( 00 mL), and acidified with 2 N HC1 (aq.) until the pH of the aqueous layer was ~4. The aqueous layer was extracted with EtO Ac (2x 50 mL). The combined organic layers were dried (Na ₂ S0 ₄ ) and filtered. After the removal of the solvents, the crude material was purified by flash chromatography (0-30% EtO Ac/heptane) to give racemic compound lf-B as a yellow oil. 'H-NMR (400 MHz, CDCI ₃ ) δ (ppm): 7.16 (t, J

------ 7.8 Hz, 1H), 6.80 (d, J ------ 8.1 Hz, IH), 6.65 - 6.75 (m, 2H), 4.87 (s, I I I). 3.99 - 4.14 (m, 2H), 2.63 - 2.80 (m, 2H), 2.26 - 2.37 (in, IH), 1.17 (t, J ------ 7.1 Hz, 3H), 0.93 - 1.08 (m,

IH), 0.51 - 0.63 (m, I H), 0.37 - 0.49 (m, 1 1 1 ). 0.27 (dq, J = 9.5, 4.9 Hz, I H), 0.15 (dq. ./ 9.5, 4.9 Hz, IH). c. (<S)-Ethyl 3-cyclopropyl-3-(3-hydroxyphenyl)propanoate (lf-S) and

(i?)-Ethyl 3-cyclopropyl-3-(3-hydroxyphenyl)propanoate (lf-R)

(lf-S) (lf-R)

The racemic mixture of compound lf-B (29.4 g) was purified by chiral SFC (Stationary phase: Chiraplpak AD 5μιη 250*30mm ), Mobile phase: 90% C0 ₂ , 10% i-PrOH) , yielding {.S ^' )-eth> 1 3-cyclopropyl-3-(3-hydroxyphenyl)propanoate (lf-S) (12.9 g, [ ] _D ²⁰ = + 35.7 c 0.5 CHCI3) and (i?)-ethyl 3-cyclopropyl-3-(3-hydroxyphenyl)propanoate (lf-R)

(13.5 g).

G. (»S)-Ethyl 3 -cyclopropyl-3 -(3 -((5 -(5, 5 -dimethylcy clopent- 1 -en~ 1 -yl)- 1 -

(2-fiuQiO-5-nieth oxyphenyl)-lH-pyrazol-4-yl)methoxy)phenyl)propanoate, Ig

To a solution of compound le (40.0 nig, 0.126 mmol), compound lf-S (44.4 mg, 0.190 mmol) and PPh. ₃ (66.3 mg, 0.253 mmol) in THF (2 mL) was added a solution of di-t-butyl azodicarboxylate (58.2 mg, 0.253 mmol) in THF (1 mL) dropwise under argon. The resulting mixture was stirred at rt for 16 h and concentrated. The residue obtained was purified by flash chromatography (0-30 % EtOAc/heptane). Compound Ig was obtained as a colorless oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for C32H37FN2O4: 533.3 (M+H); found: 533.3.

H. (5)-3-Cyclopropyl-3-(3-((5-(5,5-dimethylcyclopent-l -en- 1 -yl)- -(2-fluoro-5- methoxyphenyl)-lH-pyrazol-4-yl)methoxy)phenyl)propanoic acid, Cpd 66

To a solution of compound Ig (55.0 mg, 0.103 mmol) in THF (3 mL) and EtOH (3 mL) was added IN NaOH (3 mL, 3 mmol) solution. The reaction mixture was stirred at rt for 16 h, and then water (20 mL) was added. The pH of the mixture was adjusted to pH~4 using citric acid (2M solution). The milky solution was extracted w th DCM (2 x 20 mL). The combined organic layers were washed with brine, dried (Na ₂ S0 ₄ ), filtered and concentrated. The crude material obtained was purified by flash chromatography (0-70% EtO Ac/heptane). Compound 66 was obtained as a white solid. 'H-NMR (400 MHz,

CDC1 ₃ ) δ (ppm): 7.87 (s, IH), 7.20 - 7.28 (m, 1H), 7.08 is. ./ 9. Hz, 1H), 6.81 - 6.94 (m, 51 1 ). 5.96 (s, i l l ). 4.83 (s, 2H), 3.76 (s, 3H), 2.71 - 2.83 (m, 2H), 2.32 - 2.43 (m, 3H), 1.69 (t, J = 7.1 Hz, 2H), 0.95 - 1.09 (m, I I I ). 0.76 (s, 61 1 ). 0.57 ( id. J = 8.6, 4.0 Hz, ! ! ! }. 0.38 - 0.49 (m, ! ! i ). 0.29 (dq, ./ 9.5, 4.7 Hz, I I I ). 0.16 (dq, ./ 9.6, 4.9 Hz, i l l ). Mass

Spectrum (LCMS, ESI pos.): Calcd. for C30H33FN2O4: 505.2 (M+H); found: 505.3.

Example 2

(5)-3-Cyclopropyl-3-(3-((l -(2-fluoro-5-methoxyphenyl)-5-(2-methylprop-l-en-l-yl)-lH- pyrazol-4-yl)methoxy)phenyl)propanoic acid (Cpd 89)

A. (5-Bromo-l- -fluoro-5-methoxyphenyl)-lH-pyrazol-4-yl)methanol, 2a

The title compound was obtained using the method described in Example , Step E, from compound lc (350 mg, 1.02 mmol. Example 1, Step C). Compoiund 2a was isolated as a colorless oil. Mass Spectrum (LCMS, ESI pos.): Calcd, for CnH ₁₀ BrFN ₂ O ₂ : 301. 1 found: 301.0, 5-Bromo-4-(chloromethyl)-l -(2-fluoro-5-methoxyphenyl)-lH-pyrazole, 2b

To a solution of compound 2a (291 mg, 0.966 mmol) in DCM (10 raL) cooled in an ice- water bath was added thionyl chloride (0.140 mL, 1.93 mmol) drop wise. The mixture was stirred at 0 °C for 2 h. DCM (50 mL) was added, and the organic layer was washed with water, satd. NaHC0 ₃ , and brine. The organic layer was dried over Na ₂ S0 ₄ , filtered and concentrated to yield compound 2b as a colorless oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for d jH ₉ BrClFN ₂ G: 319,5 (M); found: 319,0. C. (S)-Methyl 3-(3-((5-bromo-l-(2-fluoro-5-methoxyphenyl)-lH-pyrazol-4-yl)

methoxy)phenyl -3-cyclopropylpropanoate, 2c

To the solution of compound 2b (300 mg, 0.939 mmol) and ( -methyl 3-cyclopropyl-3- (3-hydroxyphenyl)propanoate (248 mg, 1.13 mmol, prepared using the similar methods as described in Example 1, step F) in ACN (15 mL) was added Cs ₂ C0 ₃ (459 nig, 1.41 mmol). The mixture was stirred at rt for 16 h. EtOAc (50 mL) was added, and the organic layer was washed with water and brine, and dried over Na ₂ S0 ₄ , filtered and concentrated. The crude material was purified by flash chromatography (0-30% EtO Ac/heptane), Compound 2c was obtained as a colorless oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for

C ₂₄ H ₂₄ BrFN ₂ 0 ₄ : 503.3 (M); found: 503.3.

D. ( ₁ S)-Methyl3-cyclopropyl-3-(3-((l -(2-fluoro-5-methoxyphenyl)-5-(2-methylprop-l- en-1 -yl)-lH- pyrazol-4-yl)methoxy)phenyl)propanoate, 2d

A mixture of compound 2c (23.0 mg, 0.0457 mmol), 2,2-dimethylethenylboronic acid pinacol ester (12.5 mg, 0.0685 mmol), and K ₃ PO4 (38.8 mg, 0.183 mmol) in 1,4-dioxane (0.5 mL) and water (0.1 mL) was purged with argon.

Dichioro(diphenylphosphmoferroceiie)palladiuni (3.74 mg, 0.00457 mmol) was then added. The resulting mixture was stirred at 80 °C for 4 h. The reaction mixture was allowed to cool to RT and treated with EtOAc (50 mL). The organic layer was washed with water and brine, then dried over Na ₂ S0 ₄ , filtered and concentrated. The crude material was purified by column chromatography on silica gel (0-30% EtO Ac/heptane). Compound 2d was obtained as a colorless oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for C28H31F 2O4: 479.5 (M+H); found: 479.2,

E. (<S)-3-Cyclopropyl-3-(3-((l-(2-fluoro-5-methoxyphenyl)-5- (2-methylprop-l -en-l- yl)- lH-pyrazol-4-yl)methoxy)phenyl)propanoic acid, Cpd 89

Using the method described in Example 1, Step Η, the title compound 89 was obtained from compound 2d as a white solid (16 mg, 97 % yield). 1H-NMR (400 MHz, CDCI ₃ ) δ (ppm): 7.82 (s, I B), 7,20 - 7.25 (m, I B), 7,08 (t, 1 9,3 Hz, 1 H), 6.87 - 6.97 (m, 2B), 6,80 - 6.87 (m, 3H), 5.84 (s, 1H), 4.86 (s, 2H), 3.79 (s, 3H), 2,69 - 2.84 (m, 2H), 2.29 - 2.41 (m, I I I ). 1.80 (s, 3H), 1.57 (s, 3H), 0.96 - 1 ,09 (m, i l l ). 0.58 (tt, ./ 8.7, 4,7 Hz, ΓΗ), 0.37 - 0.48 (m, lH), 0.29 (dq, 1 9.5, 4.9 Hz, l i s ). 0.16 (dq, ,/ 9, 5, 4.9 Hz, i l l ). Mass

Spectrum (LCMS, ESI pos.): Calcd, for C27H29FN2O4: 465.5 (M+H); found: 465,3.

Following the procedures described in Example 2 above, and substituting suitably selected and substituted reagents, starting materials and conditions as would be readily apparent to those skilled in the art, the following representative compounds of the present invention were prepared. Cpc!

No, Characterization

S)-3-Cyclopropyl-3-(3-((5-cyclopropyl-l -(2-fluoro-5- methoxyphenyl)-lH-pyrazol-4-yl)methoxy)phenyl)propanoic acid

Ή-NMR (400 MHz, CDCI ₃ ) δ (ppm): 7.73 (s, 1H), 7.22 - 7.29 (m, 1H), 7.13 (t, J = 9.3 Hz, i l l). 6.90 - 7.01 (m, 2H), 6.84 -

69 6.90 (m, 3H), 4.98 (s, 2H), 3.81 (s, 3H), 2.70 - 2.85 (m, 2H), 2,31 - 2,42 (111, H i ). 1 ,74 - 1.83 (m, I I I ). 0.97 - 1.09 (m, 1H), 0.70 - 0.78 (m, 2H), 0.53 - 0.63 (m, 3H), 0.39 - 0,49 (m, IH), 0,30 (dq, J= 9.4. 4.8 Hz, IH), 0, 17 (dq. ./ 9.6. 4,9 Hz, I I I). Mass Spectrum (LCMS, ESI pos.) Calcd. For C26H27FN2O4: 451.5 ( M i l); found: 451.3,

( ^* )-3-(3-((5-Bromo-l-(2-fluoro-5-methox>'phenyl)-lH-p yrazol- 4-yl)methoxy)phenyl)-3 -cyclopropylpropanoic acid

1H-NMR (400 MHz, CDCI ₃ ) δ (ppm): 7.87 (s, IH), 7.22 ·· 7.29 (m, IH), 7.17 (t ./ 9.1 Hz, IH), 6.97 - 7,03 (m, IH), 6.95 (dd, ./ 5,6, 3.0 Hz, I H), 6,88 (s, 2H), 6.86 (s, IH), 4.96 (s, 2H),

90

3,81 (s, 3H), 2.69 - 2,86 (m, 2H), 2.30 - 2,42 (m, IH), 0.97 - 1.10 (m, IH), 0.58 (tt, J = 8.7, 4.7 Hz, IH), 0.39 - 0.49 (m, 1 1 1 ). 0.29 (dq, J 9.5, 4.7 Hz, IH), 0.17 (dq, ./ 9.7, 5.0 Hz IH). Mass Spectrum (LCMS, ESI pos.): Calcd. for C ₂ 3H ₂₂ BrFN ₂ 0 ₄ : 489.1 (M+H); found: 489.1.

Cpc!

No, Characterization

(5)-3-Cyclopropyl-3-(3-((l -(2-fluoro-5-methoxyphenyl)-5-(5- methylthiophen-2-yl)-lH-pyrazol-4- yl)methoxy)phenyl)propanoic acid

Ή-NMR (400 MHz, CDC1 ₃ ) δ (ppm): 7.87 (s, 1H), 7.20 - 7.25 (m, IH), 7.01 ·· 7.08 (m, IH), 6.90 ·· 6.99 (m, 2H), 6.84 - 6.89

94 (tti, 3! I 6.83 (d, ./ 3.5 Hz, IH), 6.64 (d, J= 2.5 Hz, IH), 4.98 (s, 2H), 3,77 (s, 3H), 2,70 - 2.84 (m, 2H), 2.41 (s, 3H), 2.31 - 2.39 (m, IH), 0,96 - 1.09 (m. IH), 0,53 - 0.64 (m, IH), 0.43 (tt, J= 8.7, 4,7 Hz, IH), 0.29 (dq, J= 9,6, 4.9 Hz, IH), 0.16 (dq, J = 9.7, 4.9 Hz, IH). Mass Spectrum. (LCMS, ESI pos.) Calcd. For C28H27FN2O4S: 507.6 (M+H); found: 507.3,

( ^, )-3-Cyclopropyl-3-(3-((5-(2,4-difluorophenyl)-l-(2-flu oro-5- methoxyphenyl)-lH-pyrazol-4-yl)methoxy)phenyl)propanoic acid

¾-NMR (400 MHz, CDCI ₃ ) δ (ppm): 7.94 (s, 111), 7.13 - 7.28 (m, 2H), 6.88 ·· 7.00 (m, 2H), 6.79 - 6.88 (m, 4H), 6.71 - 6.79

95

(m, 2H), 4.88 (s, 2H), 3.76 (s, 3H), 2.67 - 2.82 (m, 2H), 2.25 - 2.37 (m, IH), 0.92 - 1.05 (m, IH), 0.51 - 0.62 (m, IH), 0.36 - 0.47 (m, IH), 0.22 - 0.32 (m, IH), 0.13 (dq, J = 9.5, 4.9 Hz, IH). Mass Spectrum (LCMS, ESI pos.) Calcd. For C29H25F3N2O4: 523.5 (M+H); found: 523.3.

Cpc!

No, Characterization

(5)-3-Cyclopropyl-3-(3-((5-(3,5-dimethylphenyl)-l-(2-fluoro- 5- methoxyphenyl)-lH-pyrazol-4-yl)methoxy)phenyl)propanoic acid

Ή-NMR (400 MHz, CDCi ₃ ) δ (ppm): 7.91 (s, 1H), 7.21 (t, ./ 7.8 Hz, 1H), 6.90 - 7.01 (m, 3H), 6.76 - 6.88 (m, 6H), 4.90 (s,

96 2H), 3.73 (s, 3H), 2.66 - 2.82 (m, 2H), 2.26 - 2.38 (m, 1H), 2.18 (s, 6H), 0,92 - 1.06 ( m. i l l). 0.49 - 0.63 (m, I I I). 0.36 - 0,47 (nr. I I I). 0.28 (dq, J = 9.4, 4.8 Hz, I I I). 0.14 (dq, J = 9.6, 4.9 Hz, IH). Mass Spectrum (LCMS, ESI pos.) Calcd, For ( · , ! ! : i f \;.() : 515.6 (M+H); found: 515.0.

Example 3

(35)-3-Cyclopropyl-3-(3-((5-(5,5-dimethylcyclopent-l-enyl)-l -(2-fluoro-5- methoxyphenyl)- -imidazol-4-yl)methoxy)phenyl)propanoic acid (Cpd 87)

A. Ethyl 2-amino-2-cyanoacetate, 3a

To a mixture of ethyl cyano(hydroxyimino)formate (36.0 g, 253 mmol) in water (960 was added satd. sodium bicarbonate solution (240 mL) followed by addition of Na ₂ S ₂ (44.0 g, 253 mmol). After 1 h, the stirred reaction mixture was treated with another equivalent of Na ₂ S ₂ 0 ₄ (44.0 g, 253 mmol), and this was repeated two more times every 2 h. The reaction mixture was stirred for 6 h at RT and extracted with DCM (3 x 500 mL). The separated organic layers were combined and dried over Na ₂ S0 ₄ and concentrated under vacuum to give compound 3a as a yellow oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for C,H _S \■().·.: 129.1 (M+H); found: 129.1.

B. Ethyl 5-amino-l-(2-fluor -5-methoxyphenyl)-lH-imidazole-4-carboxylate, 3b

A solution of compound 3a (4.5 g, 35 mmol) and (diethoxymethoxy)ethane (6.3 g, 42 mmol) in Q¾CN (50 mL) was stirred at 80 °C for 1 h. The reaction mixture was allowed to cool to RT and treated with 2-fluoro-5-methoxyaniline (3.0 g, 21 mmol). The resulting solution was stirred overnight at RT and concentrated. The residue obtained was purified by flash chromatography (0-25% EtO Ac/petroleum ether) on silica gel to obtain compound 3b as a light yellow solid. Mass Spectrum (LCMS, ESI pos.): Calcd. for C H^FN _B O- _J : 280.1 (M+H); found: 280.0.

C. Ethyl l-(2-fluoro-5-meth xyphenyl)-5-iodo-lH-imidazole-4-carboxylate, 3e

A solution of compound 3b (500 mg, 1.79 mmol) in CFLL (3 mL) was treated with 3- methylbut l nitrite (2.097 g, 17.90 mmol) at 0 to 5 °C during 30 min under N ₂ . The resulting solution was stirred for 45 min at 100 °C in a sealed tube. The reaction mixture was allowed to cool to RT and treated with 10 mL Na ₂ S0 ₃ (satd., aq). The resulting solution was extracted with DCM (3 x 10 mL). The organic layers were combined and concentrated. The residue obtained was purified by flash chromatography on silica gel (0- 40% EtO Ac/petroleum ether) to obtain the compound 3c as a red solid. Mass Spectr um (LCMS, ESI pos.): Calcd. for C _s .·Η .;.ΙΊ >;. 391.0 (M+H); found: 391.0.

D. Ethyl 5-(5,5-dimethylcyclopent-l-enyl)-l-(2-fluoro-5-methoxyphenyl )

-lH-imidazole-4-carboxylate, 3d

A mixture of compound 3c (230 mg, 0.59 mmol), 2-(5,5-dimethylcyclopent-l-en-l-yl)- 4,4,5, 5-tetramethyl-l,3,2-dioxaborolane (262 mg, 1.18 mmol), Pd(dppf)Cl ₂ (24 mg, 0.03 mmol) and CS2CO3 (577 mg, 1.77 mmol), in dioxane (2 mL) and water (0.5 niL) was stirred overnight at 95 °C under N ₂ . The reaction mixture was allowed to cool to RT and treated with NH ₄ CI (5 mL, said., aq). The resulting solution was extracted with EtO Ac (3 x 10 mL). The organic layers were combined and concentrated. The residue obtained was purified by flash chromatography (0-40% EtO Ac/petroleum ether) on silica gel to obtain compound 3d as a light yellow oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for C20H23FN2O3: 359.2 (M+H); found: 359.1.

E. (5-(5,5-Dimethylcyclopent-l-enyl)-l -(2-fluoro-5-methoxyphenyl)-lH-imidazol-4- yl)methanol, 3e

To a solution of compound 3d (110 mg, 0.31 mmol) in THF (5 mL) was added LAH (23 mg, 0.61 mmol) at 0 °C. The resulting solution was stirred for 30 mm at 0 °C and then treated with Na ₂ S0 ₄ ^' l 0H ₂ O (2 g). The solids were removed by filtration and the filtrate was concentrated. The residue obtained was purified by flash chromatography (0-50% EtO Ac/petroleum ether) on silica gel to obtain compound 3e as a light yellow oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for C ,xl l . , l ^; N .O; _. : 317.2 (M I I ); found: 317.1.

F. 4-(Chloromethyl)-5-(5,5-dimethylcyclopent-l -enyl)-l-(2-fluoro-5- methoxyphenyl)- 1H- imidazole, 3f

3f

A solution of compound 3e (100 mg, 0.320 mmol) in DCM (2 mL) and DMF (0.05 mL) was treated with thionyl chloride (75 mg, 0.64 mmol). The resulting solution was stirred for 1 h at RT and quenched with water (5 mL). The mixture was extracted with DCM (3 x 10 mL). The organic layers were combined and concentrated to obtain compound 3f as a vellow solid.

Methyl (35)-3-cyclopropyl-3-(3-((5-(5,5-dimethylcyclopent-l -enyl)-l- (2-fluoro-5-methoxyphenyl)-lH-imidazol-4-yl)methoxy)phenyl)p ropanoate, 3;

A mixture of compound 3f (106 mg, 0.320 mmol), methyl (3<S)-3-cyclopropyl-3-(3- hydroxyphenyl)propanoate (73.0 mg, 0.330 mmol, commercially available, Synnovator Inc., Catalog # PB05716) and Cs ₂ C0 ₃ (207 mg, 0.640 mmol) in CH ₃ CN (3 mL) was stirred overnight at 50 °C. The reaction mixture was allowed to cool to RT and treated with water (10 mL). The resulting solution was extracted with EtO Ac (3 x 10 mL). The organic layers were combined and concentrated. The residue obtained was purified by flash chromatography (0-20% EtO Ac/petroleum ether) on silica gel to give compound 3g as a light yellow oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for C31H35FN2O4: 519.3 (M+H); found: 519.3.

H. (3^)-3-Cyclopropyl-3-(3-((5-(5,5-dimethylcyclopent-l-enyl)-l -(2-fluoro-5- methoxyphenyl)-lH-imidazol-4-yl)methoxy)phenyl)propanoic acid, Cpd 87

A mixture of compound 3g (90 mg, 0.17 mmol) and LiOH ^' H ₂ 0 (73 mg, 1.7 mmol) in THF (2 mL), water (1 mL) and methanol (0.5 mL) was stirred overnight at RT. The resulting mixture was concentrated to remove organic solvents. The resulting solution was diluted with H ₂ O (5 mL). The pH of the solution was adjusted to 6 with IN HQ. The solids formed were collected by filtration to give compound 87 white solid. ¹ H-NMR (400 MHz, CDCI ₃ ) δ (ppm): 7.91 (s, H i ). 7.34 - 7,39 (m, i l l). 7.19 (t, J= 7.6 Hz, 1 1 1). 7.04 - 7.07 (m, 2H), 6.82 - 6.89 (m, 3H), 6.00 (s, H i ). 4.79 (s, 2H), 2.59 - 2.68 (m, 21 1). 2.25 - 2.33 (m, 3H), 1.60 (t, ./ 6.8 Hz, 21 1). 0.98 - 1.01 (m, i l l). 0.65 (s, 6H), 0.49 - 0.51 (111, 1H), 0.22 - 0,33 (m, 2H), 0.12 - 0, 15 (m, 1H). Mass Spectrum (LCMS, ESI pos.): Calcd. for C3C.H33FN2O4: 505,2 (M+H); found: 505, 1.

Following the procedures described in Example 3 above, and substituting suitably selected and substituted reagents, starting materials and conditions as would be readily apparent to those skilled in the art, the following representative compound of the present invention was prepared.

Characterization

Cpd

No. Characterization

(5)-3-Cyclopropyl-3-(3-((l-(2-fluoro-5-methoxyphenyl)-5-(2- methylprop- 1 -en- 1 -y ])- lH-imidazol-4- yl)methoxy)phenyl)propanoi c acid

Ή-NM (300 MHz, OM$Q-d ₆ ) δ (ppm): 11.52 - 12.33 (brs, Hi).8.12 (s, 1H), 7,48 - 7.43 (m. III).7,07 - 7.22 (m.3H),

91 6.82 - 6.88 (m, 3H), 5,79 (s, III).4,84 (s, 2H), 3.78 (s, 3H), 2.62 - 2,66 (m, 2H), 2.24 - 2.29 (m, ill).1.77 is.311).1.56 (s, 311).0.98 - 1,03 (in, ill).0.47 - 0.51 (m, ill).0.22 - 0.34 (m, 2H), 0,10 -0.15 (m, 1H),

Mass Spectrum (LCMS, ESI pos.): Calcd. for C27H29FN2O4:

465.2 (M+H); found: 465.2,

Example 4

(35)-3-Cyclopropyl-3-(3-((4-cyclopropyl-5-(2-fluoro-5-methox yphenyl)isoxazol-3- 37)

A mixture of 2-bromo-l-fluoro-4-methoxybenzene (5.0 g, 24 mmol),

(ethenyloxy)methanol (9.0 g, 122 mmol), Pd(OAc) ₂ (110 mg, 0,49 mmol), DPPP (480 m 1.16 mmol) and potassium carbonate (10 g, 72 mmoi) in water (50 mL) was stirred overnight at 90 °C under N ₂ . The reaction mixture was allowed to cool to RT and then concentrated HCl (10 ml.) was added. The resulting mixture was stirred for 30 rain, and then was extracted with EtOAc (3 x 50 mL). The organic layers were combined and washed with H ₂ 0 (2 x 100 mL), dried over Na ₂ S0 ₄ and concentrated. The residue obtained was purified by flash column chromatography (0-10% EtO Ac/petroleum ether) on silica gel to obtain compound 5a as a colorless oil. Ή-NMR (300 MHz, DMSO-</ ₆ ) δ (ppm): 7.18 - 7.33 (m, 3H), 3.79 (s, 3H), 2.58 (d, ./ 4.5 Hz, 3H). Mass Spectrum

(GCMS, EI): Calcd. for C ₉ H ₉ F0 ₂ : 168.1 (M); found: 168.2.

B. Ethyl 4-(2-fluoro-5-methoxyphenyl)-2,4-dioxobutanoate, 4b

4b

A mixture of l-(2-fluoro-5-methoxyphenyl)ethan-l -one (4a) (2.6 g, 15 mmol), diethyl oxalate (2.7 g, 18 mmol) and sodium hydride (60% in mineral oil, 1 .2 g, 30 mmol) in DMF (5 mL) was stirred overnight at RT. The reaction was then quenched by the addition of said. NH ₄ C1 (aq., 5 mL). The resulting solution was extracted with EtO Ac (3 x 50 mL). The organic layers were combined and washed with H ₂ 0 (2 x 10 mL), dried over Na ₂ S0 ₄ and concentrated. The residue obtained was purified by flash column chromatography (EtO Ac/petroleum ether 1 :6) on silica gel to obtain compound 4b as a white solid. Mass Spectrum (LCMS, ESI pos.): Calcd. for CV.i ! _Γ ,!·Χ),: 269.1 (M+H); found: 269.1.

C. Ethyl 5-(2-fluoro-5-methoxyphenyl)isoxazole-3-carboxylate, 4e

A solution of compound 4b (2, 5 g, 9.3 mmol) and NH ₂ OH.HCl (836 mg, 12.1 mmol) in ethanol (15 mL) was heated at reflux for 2 h. The reaction mixture was allowed to cool to RT and treated with satd. NH ₄ CI (aq., 20 mL). The resulting solution was extracted with EtOAc (3 x 30 mL). The organic layers were combined and washed with H ₂ 0 (2 x 50 mL), dried over Na ₂ S0 ₄ and concentrated. The residue obtained was purified by flash column chromatography (EtO Ac/petroleum ether 1 : 15) on silica gel to obtain compound 4c as a white solid. Mass Spectrum (LCMS, ESI pos.): Calcd. for Ci 3H ₁₂ FN0 ₄ : 266.1 (M+H); found: 266.1.

(5-(2-Fluoro-5-methoxyphenyl)isoxazol-3-yl)methanol, 4d

To a solution of compound 4e (2.2 g, 8.3 mmol) in THF (20 mL) was added DIBAL-H (1 M in hexane, 41 mL, 41 mmol) dropwise with stirring at -30 °C. The resulting solution was stirred for 1 h at RT. The reaction was then quenched by the addition of satd. sodium potassium tartrate solution (aq., 30 mL). The resulting solution was extracted with EtO Ac (3 x 50 mL). The combined organic layers were washed with H ₂ 0 (2 x 70 mL), dried (Na ₂ S0 ) and concentrated. The residue obtained was purified by flash column chromatography (0-10% EtOAc/DCM) on silica gel to obtain compound 4d as a yellow solid. Ή- MR (300 MHz, DMSO-tfe) δ (ppm): 7.34 - 7.40 (in, 211), 7.09 - 7.15 (m, 1 H), 6.88 (d, J = 3.3 Hz, I I I ). 5.59 (t, J = 6.3 Hz, lH), 4.60 (d, J = 6.3 Hz, 2H), 3.84 (s, 3H).

E. (4-Bromo-5-(2-fluoro-5-methoxyphenyl)isoxazol-3-yl)methanol, 4e

A solution of compound 4d (1.07 g, 4.79 mmol) and NBS (1.02 g, 5.73 mmoi) in DMF (10 mL) was stirred overnight at 60 °C. The reaction mixture was allowed to cool to RT and treated with satd. NH ₄ CI (aq. 20 mL). The resulting solution was extracted with EtOAc (3 x 20 mL). The combined organic layers were washed with H ₂ 0 (2 x 30 mL), dried over Na ₂ S0 ₄ and concentrated. The residue obtained was purified by Flash-Prep-HPLC using CH ₃ CN and water as the mobile phase to give compound 4e as a yellow solid. Mass Spectrum (LCMS, ESI pos.): Calcd. for CnH ₉ BrFN0 ₃ : 302.0 (M I I); found: 302.0.

F. 4-Bromo-3-(chloromethyl -5-(2-fluoro-5-methoxyphenyl)isoxazole, 4f

To a solution of compound 4e (710 mg, 2.35 mmol) in DCM (5 mL) and DMF (0.2 mL) was added thionyl chloride (1.32 g, 7.02 mmol) dropwise. The resulting solution was stirred for 1 h at RT. The reaction was then quenched by the addition of satd. NH4CI (aq., 5 mL). The resulting solution was extracted with EtOAc (3 x 5 mL). The combined organic layers were washed with H ₂ 0 (2 x 10 mL), dried over Na ₂ S0 ₄ and concentrated to give compound 4f as a yellow oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for

Cii¾BrClFN0 ₂ : 319,9 (M+H); found: 319.8.

G. (3«S)-Methyl 3-(3-((4-bromo-5-(2-fluoro-5-methoxyphenyl)isoxazol-3-yl)

methoxy)pheny -3-cyclopropylpropanoate, 4g

A mixture of 4-bromo-3-(chloromethyl)-5-(2-fluoro-5-methoxyphenyl)isoxazo le (cpd 4f, 360 mg, 1.12 mmol), methyl (3 _i )-3-cyc3opropyl-3-(3-hydroxyphenyl)propanoate (271 mg. 1.23 mmol) and CS2CO3 (731 mg, 2.24 mmol) in DMF (2 mL) was stirred for 1 h at 80 °C. The reaction mixture was allowed to cool to RT and treated with satd. NH ₄ CI (aq., 5 ml.). The resulting solution was extracted with EtOAc (3 x 5 mL). The combined organic layers were washed with H ₂ 0 (2 x 10 mL), dried over Na ₂ S0 and concentrated. The residue obtained was purified by flash column chromatography (0-10% EtO Ac/petroleum ether) on silica gel to obtain compound 4g as a yellow oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for C ₂₄ H ₂₃ BrFN0 ₅ : 504.1 (M+H); found: 504.1, 506.1 .

H. (35)-Methyl 3-cyclopropyl-3-(3-((4-cyclopropyl-5-(2-fluoro-5- methoxyphenyl)isoxazol-3-yl)methoxy)phenyl)propanoate, 4h

A mixture of compound 4g (200 mg, 0.40 mmol), cyclopropylboronic acid (36 mg, 0.42 mmol ), PdCl ₂ dppf (30 mg, 0.04 mmol) and Cs ₂ C0 ₃ (260 mg, 0.80 mmol) in dioxane (4 mL) and water (1 mL) was stirred at 90 °C for 2 h under N ₂ in a sealed tube. The reaction mixture was allowed to cool to RT and treated with satd. NH ₄ C1 (aq., 5 mL). The resulting solution was extracted with EtO Ac (3 x 10 mL). The organic layers were combined and washed with water (30 mL) and concentrated. The residue obtained was purified by flash column chromatography (0-10% EtO Ac/petroleum ether) on silica gel to obtain compound 4h as a yellow liquid. Mass Spectrum (LCMS, ESI pos.): Calcd. for CH I .*l ^; \0<: 466.2 (M+H); found: 466.2.

I. (3S)-3-Cyclopropyl-3-(3-((4-cyclopropyl-5-(2-fluoro-5-methox yphenyl)isoxazol-3- yi)methoxy)phenyl)propanoic acid, Cpd 37

The compound 37 was prepared from compound 4h following the procedure described in Example 1, Step H. The solid obtained was purified by Prep-HPLC (eluting with water and CH ₃ CN) gave compound 37 as a white solid. Ίί-NMR (300 MHz, DMSO-d ₆ ) δ (ppm): 7.33 - 7.39 (m, 1H), 7.15 - 7.25 (m, 3H), 6.88 - 6.96 (m, 3H), 5.21 (s, 2H), 3.81 (s, 3H), 2.51 - 2,60 (m, 2H), 2.29 - 2.38 (m, IH), 1.74 - 1.77 (m, IH), 0.96 - 0.99 (m, IH), 0.74 ·· 0.80 (m, 2H), 0.44 ·· 0.48 (m, IH), 0.31 - 0.39 (m, 2H), 0.20 - 0.30 (m, 2H), 0.05 - 0.12 (m, IH). Mass Spectrum (LCMS, ESI pos.): Calcd. for C\,j I ,,i \C) 452.2 (M+H); found: 452.1.

Following the procedures described in Example 4 above, and substituting suitably selected and substituted reagents, starting materials and conditions as would be readily apparent to those skilled in the art, the following representative compounds of the present invention were prepared.

Cpd No. Characterizatioil

( _i S ^' )-3-Cyclopropyl-3-(3-((5-(2-fluoro-5-methoxyphenyl)-4- (3 -methylthi ophen-2-y 1) isoxazol -3 - yl)methoxy)phenyl)propanoic acid

¹ H-NMR. (400 MHz, OM$Q-d ₆ ) δ (pprn): 12.00 (brs, 1H), 7,56 - 7,58 (m, IH), 7.31 - 7,33 (m, 1H), 7.11 - 7, 1 8 (m, 2H),

33 6.77 - 6.98 (ra, 5H), 5.09 (s, 2H), 3,65 (s, 3H), 2,61 - 2.67 (m, 2H), 2.23 - 2.50 (m, IH), 1.85 (s, 3H), 0.97 - 0.98 (m, ! ! ! }. 0.31 - 0,48 (m, IH), 0.21 - 0,29 (in. 2H), 0.00 - 0.12 (m, 2H).

Mass Spectrum (LCMS, ESI pos. ): Calcd. for C; _S 1 I .„I ^; \0<S. 508.2 (M+H); found: 508.0.

(5)-3-Cyclopropyl-3-(3-((5-(2-fluoro-5-methoxyphenyl)-4- phenylisoxazol-3-yl)methoxy)phenyl)propanoic acid

' ! I-N R (300 MHz, OMSO-d ₆ ) δ (pprn): 7,29 - 7.39 (m, 5H), 7.10 - 7.26 (m, 3H), 7.03 - 7.09 (m, IH), 6.79 - 6.87 (m,

34 3H), 5, 17 (s, 2H), 3.69 (s, 3H), 2.55 - 2,59 (nr. 2H), 2.23 - 2,32 (rn, IH), 0.94 - 0.97 (rn, IH), 0,46 - 0.48 (m, I H), 0.21 - 0.30 (m, 2H), 0,07 - 0, 1 1 (m, I H),

Mass Spectrum (LCMS, ESI pos,): Calcd, for C _; i k.FNO 488, 2 (M+H); found: 488,3.

Cpd No. Characterizatioil

(3 _i .S ¹ )-3-Cyclopropyl-3-(3-((5-(2-fluoro-5-methoxyphenyl)-4- (2-methylprop-l-enyl)isoxazol-3- yl)methoxy)phenyl)propanoic acid

i f-NMR (300 MHz, DMSO-d ₆ ) δ (ppm): 7,30 - 7,37 (m, IH), 7.1 1 - 7.22 (m, 3H), 6.82 - 6.88 (m, 3H), 5.99 (s, i ll),

36

5.1 1 (s, 2H), 3.77 (s, 3H), 2.43 - 2,45 (m, 2H ), 2,29 - 2.35(m, 111), l,80(s, 3H), 1.30(s, 3H), 0.90 - 1.05 (m, IH), 0.44 - 0.54 (m, IH), 0.21 - 0.40 (m, 2H), 0.02 - 0.18(m, IH). Mass Spectrum (LCMS, ESI pos.): Calcd. for C ₂₇ H ₂₈ FNOs: 466.2 (M+H); found: 466.2.

(3S)-3-(3-((4-(5-teri-butylthiophen-2-yl)-5-(2-fluoro-5- methoxyphenyl)isoxazol-3-yl)methoxy)phenyl)-3- cyclopropylpropanoic acid

1H-NMR (300 MHz. DMSO-d ₆ ) δ (ppm): 7,32 - 7.36 (m, 2H), 7.1 1 - 7.29 (m, 2H), 7.04 - 7.10 (m, I H), 6,84 - 6.96 (m,

45

3H), 6.79 (s, IH), 5.19 (s, 2H), 3.72 (s, 3H), 2.58 - 2,60 (m, 2H), 2.26 - 2.28 (m, IH), 1.19 (s, 9H), 0.98 (s, I H), 0.40 - 0.48 (m, I H), 0,23 - 0.27 (m, 2H), 0.1 1 (s, IH). Mass Spectrum (LCMS, ESI pos,): Calcd. for C ₃₁ H ₃₂ FNO ₅ S: 550.2 (M-i-H); found: 550.2.

Example 5

(36)-3-Cyclopropyl-3-(3-((5-(5,5-dimethylcyclopent-l -enyl)-l-(2-fluoro-5- methoxyphenyl)-lH- -triazol-4-yl)methoxy)phenyl)propanoic acid (Cpd 86)

A. 2-Azido~l~fiuoro~4~methoxybenzene, 5a

To a mixture of 2-fluoro-5-methoxyaniline (5.0 g, 35 rnmol), sulfuric acid (7.5 mL), trifluoroacetic acid (37.5 mL) and water (45 mL) was added NaN0 ₂ (3.7 g, 53 mmol) portionwise at 0 °C. The mixture was stirred for 30 min at 0 °C and NaN ₃ (4.6 g, 71 mmol) was added. The resulting solution was stirred overnight at RT and extracted with EtOAc (2 x 200 mL). The combined organic layers were dried over Na ₂ S0 ₄ and concentrated. The residue obtained was purified by column chromatography on silica gel (EtO Ac/petroleum ether, 1 : 100) to give compound 5a as a light yellow oil. ¹ H- vtR (300 MHz. DMSO-d ₆ ) δ (ppm): 7,23 (dd, ./,· = 9,0 Hz, ,/· = 10.8 Hz, i l l). 6.72 - 6.80 (m, 21 1). 3.76 (s, 3H). Mass Spectrum (GCMS, EI): Calcd. for C^FNsO: 167,0 (M); found; 167.2.

B. Ethyl 5-amino-l -(2-fluor -5-methoxyphenyl)-l H-l,2,3-triazole-4-carboxylate, 5b

To a solution of EtONa (4.2 g, 62 mmol) in ethanol (30 mL) was added ethyl 2- cyanoacetate (6.5 g, 57 mmol) at 0 °C. The resulting mixture was stirred for 30 min and treated with compound 5a (4.8 g, 29 mmol). The resulting solution was stirred for 3 h at RT and quenched with water (100 mL). The mixture was extracted with EtOAc (2 x 100 mL). The combined organic layers were dried (Na ₂ S0 ₄ ) and concentrated. The residue obtained was purified by column chromatography on silica gel (EtO Ac/petroleum ether, 1 : 1) to give compound 5b as a white solid. Mass Spectrum (LCMS, ESI pos.): Calcd. for (Ϊ.! ! I ;!- \ 0-.: 281.1 (M i l ); found: 281.1.

C. (5-Amino-l-(2-fluoro-5-methoxyphenyl)-lH-l,2,3-triazol-4-yl) methanol, 5e

To a solution of compound 5b (4.0 g, 14 mmol) in THF (60 mL) was added DIBAL-H (1 M in hexane, 71 mL, 71 mmol) at -70 °C. The resulting solution was stirred for 2 h at -70 °C. The reaction was then quenched by satd. sodium potassium tartrate solution (aq., 100 mL). The resulting solution was extracted with EtO Ac (2 x 100 mL). The combined organic layers were dried (Na ₂ S0 ₄ ) and concentrated. The residue obtained was purified by column chromatography on silica gel (EtO Ac/petroleum ether, 100: 1) to give compound 5c as a colorless oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for C] _. oHuFN ₄ 0 ₂ : 239.1 (M+H); found: 239.1.

D. (5-Bromo-l -(2-fluoro-5-methoxyphenyl)-l H-l ,2,3-triazol-4-yl)methanol, 5d

To a stirred mixture of CuBr ₂ (5.6 g, 25 mmol) and t-BuONO (7.8 g, 76 mmol) in C¾CN (50 mL) under N ₂ was added compound 5c (3.0 g, 13 mmol) in CJ¾CN (10 mL) dropwise. The resulting solution was stirred for 2 h at 0 °C and treated with satd. NH ₄ CI (aq. 100 mL). The mixture was extracted with EtO Ac (2 x 100 mL). The combined organic layers were dried (Na ₂ S0 ₄ ) and concentrated. The residue obtained was purified by column chromatography on silica gel (EtO Ac/petroleum ether, 1 : 3) to give compound 5d as a colorless oil. Mass Spectrum (LC MS. ESI pos.):Calcd. for C ₁₀ H ₉ B1-FN ₃ O ₂ : 302.0 (M i l); found: 301.9.

E. 5-Bromo-4-(chloromethyl -l-(2-fluoro-5-methoxyphenyl)-lH-l,2,3-triazole, 5e

To a solution of compound 5d (600 mg, 1.98 mmol) in DCM (6 mL) and DMF (1 drop) was added thionyl chloride (703 mg, 5.96 mmol) dropwise at 0 °C. The resulting solution was stirred for 1 h at RT and concentrated to give compound 5e as a colorless oil.

(35)-Ethyl 3-(3-((5-bromo-l-(2-fluoro-5-methoxyphenyl)-lH-l,2,3-triazol -4- methoxy)phenyl)-3-cyclopropylpropanoate, 5f

A mixture of compound 5e (170 mg, 0.530 mmol), Cs ₂ C0 ₃ (434 mg, 1.33 mmol) and ethyl (35)-3-cyclopropyl-3-(3-hydroxyphenyl)propanoate (130 mg, 0.550 mmol, as prepared in Example 1 , Step F) in DMF (4 ml.) was stirred overnight at 60 °C, The reaction mixture was allowed to cool to RT and treated with water (10 mL). The resulting mixture was extracted with EtO Ac (2 x 10 mL). The combined organic layers were dried (Na ₂ S0 ₄ ) and concentrated. The residue obtained was purified by column chromatography on silica gel (EtO Ac/petroleum ether, 1 :3) to give compound 5f as a colorless oil. Mass Spectrum (LCMS, ESI pos.): Calcd. ior ^BrFN -.(),· 518, 1 (M+H); found: 518.1. (35')-Eihyl 3-cyclopropyl-3-(3-((5-(5,5-dimethylcyclopent-l-enyl)-l-(2-f l ^" methoxyphenyl)- -l,2,3-triazol-4-yl)methox>')phenyl)propanoate, 5g

A mixture of compound 5f (120 mg, 0.23 mmol), 2-(5,5-dimethylcyclopent-l-en-l -yl)- 4,4,5, 5-tetramethyl-l ,3,2-dioxaborolane (77 mg, 0.35 mmol), sodium carbonate (61 mg, 0.58 mmol), Pd(dppf)Cl ₂ (8.4 mg, 0.01 mmol) in dioxane (4 mL) and water (1 mL) was stirred overnight at 60 °C. The reaction mixture was allowed to cool to RT and treated with water (10 mL). The resulting solution was extracted with EtOAc (2 x 10 mL). The combined organic layers were dried (Na ₂ SO.}) and concentrated. The residue obtained was purified by column chromatography on silica gel with EtO Ac/petroleum ether, 1 :3) to give compound 5g as a colorless oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for

C31H36FN3O4: 534.3 (M+H); found: 534,4. H. (3 _i S ^' )-3Cyclopropyl-3-(3-((5-(5,5-dimethylcyclopent-l-enyl) -l -(2-fluoro-5- methoxyphenyl)-! H-l,2,3-triazol-4-yl)methoxy)phenyl)propanoic acid, Cpd 86

Compound 86 was prepared from compound 5g following the procedure described in

Example 3, Step 1 1. ' i l-NMR (300 MHz, DMSO-d ₆ ) δ (ppm): 7.41 - 7,50 (m, IH), 7.18 -

7,31 (m, 3H), 6.75 - 6,98 (m, 3H), 6.08 (s, IH), 5.02 (s, 2H), 3.77 (s, 3H), 2.60 - 2.70 (m, 2H), 2,22 - 2.41 (m, 3H), 1.61 - 1.72 (m, 2H), 0,92 - 1.09 (m, IH), 0,65 - 0,80 (s, 6H), 0,41

- 0.59 (m, IH), 0.21 - 0.36 (in, 2H), 0,04 - 0.13 (m, IH), Mass Spectrum (LCMS, ESI pos.):Calcd. for C29H32FN3O4: 506,2 (M+H); found: 506, 1.

Following the procedures described in Example 5 above, and substituting suitably selected and substituted reagents, starting materials and conditions as would be readily apparent to those skilled in the art, the following representative compounds of the present invention were prepared. Cpd No. Characterization

(35)-3-Cyclopropyl-3-(3-((l -(2-fluoro-5-methoxyphenyl)-5-(2- methylprop- 1 -enyl)- 1H- 1 ,2,3 -triazol-4-yl)methoxy)phenyl) propanoic acid

1H-NMR (300 MHz, DMSO-de) δ (ppm): 1 1.85 - 12.21 (s, 1 H), 7.41 - 7,51 (t, I B), 7. 15 - 7,31 (m, 3H), 6.78 - 7.01 (m, 3H),

84 5.88 - 5.92 (s, I II), 5.00 - 5, 1 1 (s, 2H), 4.78 - 4.88 (s, 3H), 2,61 - 2,75 (m, 2H), 2.20 - 2.32 (m. Ill), 1.80 - 1.85 (s, 311), 1.50 - 1.56 (s, 3H), 0.95 - 1.11 (m, 1H), 0.42 - 0.58 (m, 111), 0.22 - 0.38 (m, 211), 0.10 - 0.1 8 (m, III).

Mass Spectrum (LCMS, ESI pos.): Calcd. for (\j h.;i ^; \ ~,().j: 466.2 (M+H); found: 466.0.

(3S)-3-Cyclopropyl-3-(3-((5-cyclopropyl-l-(2-fluoro-5- methoxy phenyl) - 1H- 1 ,2,3 -triazol-4- yl)methoxy)phenyl)propanoic acid acid

1H-NMR (300 MHz, DMSO-de) δ (ppm): 7.52 - 7,61 (t, IH),

7.31 - 7,35 (in, IH), 7.20 - 7.28 (rn, 2H), 6.85 - 7.01 (m, 3H),

85 5.12 - 7.19 (s, 2H), 4.78 - 4.84 (s, 311), 2,58 - 2.71 (m, 211),

2.22 - 2,33 (in, IH), 1 .71 - 1.82 (rn, IH), 0.91 - 1.05 (m, I H),

0.80 - 0.88 (m, 2H), 0,69 - 0, 72 (m, 2H), 0,40 - 0.52 (t, IH),

0.20 -0.31 (m, 211), 0.1 1 -0.19 (t, 111).

Mass Spectrum (LCMS, ESI pos.): Calcd. for -! ί ,,i ^; \ ~.C) ₄ :

451.2 (M+H); found: 452.0.

Cpd No. Characterization

(35)-3-Cyclopropyl-3-(3-((l -(2-fluoro-5-methoxyphenyl)-5-(5- methylthiophen-2-yl)-l.H-l,2,3-triazol-4-yl)methoxy)phenyl) propanoic acid

1H-NMR (300 MHz, DMSO-d ₆ ) δ (ppm): 7,26 - 7.27 (ra, 1 H), 7.1 1 - 7.16 (m, I H), 6,92 - 7.12 (ra, 5H), 6.87 - 6.89 (d, 1 2.7

88 Hz, 1H), 6.69 (s, 1H), 5.24 (s, 2H), 3.81 (s, 3H), 2.78 - 2,85 (m, 2H), 2.31 - 2.42 (m, 4H), 1.01 - 1.11 (in. IH), 0.55 - 0.65 (m, IH), 0.40 - 0.49 (m, IH), 0.25 - 0.33 (m, IH), 0.14 - 0.22 (m, 1H).

Mass Spectrum (LCMS, ESI pos.): Calcd. for (Vf hj- ^' N -.OjS: 509.1 (M+H); found: 509.1.

(3S)-3-(3-((5-(5-ieri-Butylthiophen-2-yl)-l-(2-fluoro-5- methoxy phenyl)- IH -1 ,2, 3 -triazol-4 -yl)methoxy)phenyl)-3 - cy clopropy lpropanoi c acid

¾-N R (300 MHz, D SO-de) δ (ppm): 7.33 - 7.45 (rn, 3H), 7.19 - 7,24 (m, 2H), 6.86 - 6.95 (rn, 3H), 6.71 (s, IH), 5.19 (s,

92

2H), 2.79 (s, 3H), 2.62 - 2,65 (m, 2H), 2.24 - 2.27 (m, IH), 1.20 (s, 9H), 0.96 - 1.00 (m, IH), 0.47 - 0,50(m, I H), 0,21 - 0.32 (m, 2H), 0.04 - 0, 1 1 (m, IH).

Mass Spectrum (LCMS, ESI pos.): Calcd. for C ₃ 0H32 N3O4S: 551.7 (M+H); found: 551.7.

Example 6

(35)-3-Cyclopropyl-3-(3-((5-(2-fluoro-5-methoxyphenyl)-4-iso butylisoxazol-3

yl)methoxy)phenyl)propanoic acid (Cpd 35)

A. 4-Bromo-3-(((tert-bu1yldimethylsilyl)oxy)methyl)-5-(2-fluoro -5-methoxyphenyl) isoxazole, 6a

To a solution of [4-bromo-5-(2-fluoro-5-methoxyphenyl)-l ,2-oxazol-3-yl]methanol (2 g, 6.6 mmol, as prepared in Example 4, Step E) in DCM (10 ttiL) was added DMAP (71 rng, 0.58 mmol) and triethylamine (1.34 g, 13.3 mmol), followed by TBSCl (1.33 g, 8.87 mmol). The resulting solution was stirred overnight at RT. The reaction was then quenched by satd. NH4CI (aq. 10 mL). The resulting solution was extracted with EtOAc (3 x 10 mL). The organic layers were combined, washed with H ₂ 0 (2 x 1 5 ml.), and concentrated. The residue obtained was purified on by column chromatography on silica gel, eluting with petroleum ether, to give compound 6a as a yellow liquid. Mass Spectrum (LCMS, ESI pos.): Calcd. for CY-! BiT Ni^Si. 416.1 ( M · ! ! }; found: 416.0.

B. 3-((tert-Bu1yldimethylsilyloxy)methyl)-5-(2-fluoro-5-methoxy phenyl)-4-(2- methylprop-1 -enyl)isoxazole, 6b 6b

A mixture of compound 6a (250 nig, 0.60 mmol), 4,4,5, 5-tetramethyl-2-(2-methylprop-l- en-l-yl)-l,3,2-dioxaborolane (164 mg, 0.90 mmol), Pd(dppf)Cl ₂ (44 mg, 0.06 mmol) and CS2CO3 (489 mg, 1.50 mmol) in dioxane (2 niL) and water (0.5 mL) was stirred for 3 h at 95 °C under N ₂ . The reaction mixture was allowed to cool to RT and treated with water (15 mL). The resulting solution was extracted with EtOAc (3 10 mL). The organic layers were combined, dried over anhydrous sodium sulfate and concentrated. The residue obtained was purified by column chromatography on silica gel (EtO Ac/petroleum ether, 1 : 100-1 :20) to give compound 6b as a colorless oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for C21H ₃₀ FNO ₃ S1: 392.2 (M+H); found: 392.2.

C. 3-((tert-Butyldimethylsilyloxy)methyl)-5-(2-fluoro-5-methoxy phenyl)-4- isobutylisoxazole, 6c

Compound 6b (150 mg, 0.38 mmol) was hydrogenated over 10% Pd/C (150 mg) for 5 h under 1 atm H ₂ at RT. The reaction mixture was filtered through a pad of diatomaceous earth and the filtrate was concentrated. The residue obtained was purified by column chromatography on silica gel (EtO Ac/petroleum ether, 1 : 100-1 :20) to give compound 6e as a colorless oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for C21H32FNO3S1: 394.2 (M+H); found: 394.1. D. (5-(2-Fluoro-5-methoxyphenyl)-4-isobutylisoxazol-3-yl)methan ol, 6c!

To a solution of compound 6c (120 mg, 0.30 mmol) in THF (5 mL) was added TBAF (1M in THF, 0.82 mL). The resulting solution was stirred for 1 h at RT. The reaction was then poured into water (10 mL). The resulting solution was extracted with EtOAc (3 x 10 mL). The organic layers were combined, dried over anhydrous sodium sulfate and concentrated. The residue obtained was purified by column chromatography on silica gel

(EtO Ac/petroleum ether, 1 : 100-1 : 10) to give compound 6d as a colorless oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for C _{i 5} H _ls FN0 ₃ : 280.1 (M+H); found: 280.0.

E. 3-(Chloromethyl)-5-(2-fluor -5-methoxyphenyl)-4-isobutylisoxazole, 6e

To a stirring solution of compound 6d (90 mg, 0.32 mmol) in DCM (5 mL), and DMF (0.2 mL) was added thionyl chloride (115 mg, 0.98 mmol) dropwise at 0 °C. The resulting solution was stirred for 1 h at 0 °C. The reaction was then quenched with water (10 mL). The resulting solution was extracted with DCM (3 x 10 mL). The organic layers were combined, dried over anhydrous sodium sulfate and concentrated to give compound 6e as a colorless oil. Mass Spectrum (LCMS, ESI pos.): Calcd, for C , d I , -(ΊΓ Κ 298.1 (M+H); found: 298.1. F. (35')-Eihyl 3-cyclopropyl-3-(3-ff5-f2-fluoro-5-methoxyphenyl) -4-isobutylisoxaz -3-yl)methoxy)phenyl)propanoate, 6f

A mixture of compound 6e (85 mg, 0.29 mmol), compound If (75 mg, 0.32 mmol) and Cs ₂ CO; (168 mg, 0.52 mmol) in CH ₃ CN (5 mL) was stirred overnight at 50 °C. The reaction mixture was allowed to cool to RT and treated with water (10 mL). The resulting solution was extracted with EtOAc (3 x 10 mL). The organic layers were combined, dried over anhydrous sodium sulfate and concentrated. The residue obtained was purified by column chromatography on silica gel (EtO Ac/petroleum ether, 1 : 00- 1 : 10) to give compound 6f as a colorless oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for

C29H34FNO5: 496.2 (M+H); found: 496.3.

G. (3S)-3-Cyclopropyl-3-(3-((5-(2-fluoro-5-methoxyphenyl)-4-iso butylisoxazol-3- yl)methoxy)phenyl)propanoic acid, Cpd 35

Compound 35 was prepared from compound 6f following the procedure described in Example 3, Step 1 1. Ή-NMR (300 MHz, DMSO-d ₆ ) δ (ppm): 7.37 (t, J ------- 9.6 Hz, I I I ).

7.12 - 7.25 (m, 3H), 6,87 - 6.94 (m, 3H), 5,20 (s, 2FI), 3,80 (s, 3H), 2.51 - 2.57 (m, 2H), 2.42 - 2,49 (m, 2H), 2.28 - 2.31 (m, IH), 1 .72 - 1.75 (m, IH), 0.90 - 1.05 (m, 1 H), 0.74 (s, 3H), 0.71 (s, 3H), 0.44 - 0.54 (m, IH), 0.21 - 0.40 (m, 2H), 0.02 - 0.18(m, IH). Mass Spectrum (LCMS, ESI pos.): Calcd. for C27H ₃ 0FNO5: 468.2 ( M 1 1 ); found: 468.2,

Example 7

(35)-3-Cyclopropyl-3-(3-((4-(5,5-dimethylcyclopent-l-enyl)-3 -(2-fluoro-5- methoxyphenyl)isoxazol-5-yl)methoxy)phenyl)propanoic acid (Cpd 62)

A. 2-Fluoro-5-methoxybenzaldehyde, 7a

To a stirring solution of 2-bromo-l -fluoro-4-methoxybenzene (6.5 g, 32 mmol) in THF (40 mL) was added n-BuLi (2.5M in THF, 15.2 mL) dropwise at -78 °C under N ₂ . The resulting mixture was stirred for 30 min and treated with DIVIF (3.7 mL, 48 mmol) dropwise with stirring at -78 °C. The resulting solution was stirred for 1 h at RT. The reaction was then quenched by satd. NH4CI solution (aq. 30 mL). The resulting solution was extracted with EtOAc (3 x 30 mL). The organic layers were combined, dried over anhydrous sodium sulfate and concentrated to give compound 7a as a yellow oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for C _s H ₇ F0 ₂ : 155.0 (M+H); found: 155.1.

B. (iT)-2-Fluoro-5-methoxybenzaidehyde oxime, 7b

A mixture of compound 7a (6.0 g, 39 mmol), KOAc (7.6 g, 78 mmoi) and NH ₂ OH.HCl (4.0 g, 58 mmol) in ethanol (70 mL) was stirred for 2 h at 80 °C. The reaction mixture was allowed to cool to RT and treated with water (100 mL). The resulting solution was extracted with EtOAc (3 x 60 mL). The organic layers were combined, dried over anhydrous sodium sulfate and concentrated. The residue obtained was purified by column chromatography on silica gel (DCM/methanol, 100: 1) to give compound 7b as a light yellow solid. Mass Spectrum (LCMS, ESI pos. ): Calcd. for C ₈ i¾FN() ₂ : 170.0 (M i l ) found: 170.1. C. 2-Fluoro-N-hydroxy-5-methoxybenzimidoyl chloride, 7c

7c

To a stirring solution of compound 7b (5.0 g, 30 mmol) in DMF (20 mL) was added dropwise a solution of NCS (4.3 g, 32 mmoi) in DMF (10 mL) at 0 °C. The resulting solution was stirred for 2 h at RT and treated with water (100 mL). The mixture was extracted with EtOAc (3 x 50 mL). The organic layers were combined and washed with water (3 x 100 mL). The organic layer was dried over anhy drous sodium sulfate and concentrated to give compound 7c as a yellow oil. Mass Spectrum (LCMS, ESI pos. ): Calcd. for C _s l K ll ^' NC 204.0 (M+H); found: 204.0. D. Methyl 3-(2-fluoro-5-methoxyphenyl)isoxazole-5-carboxylate, 7d

A mixture of compound 7c (4.5 g, 22 mmol) and methyl prop-2-ynoate (2.2 g, 26 mmol) in toluene (70 mL) was treated with TEA (2.7 g, 26 mmol) dropwise with stirring at 0 °C. The resulting solution was stirred for 1 h at RT. The reaction was then quenched by water (100 mL). The resulting solution was extracted with EtOAc (3 x 50 mL). The organic layers were combined, dried over anhydrous sodium sulfate and concentrated. The residue obtained was purified by column chromatography on silica gel (EtO Ac/petroleum ether, 1 : 100-1 :20) to give compound 7d as a light yellow solid. Mass Spectrum (LCMS, ESI pos. ): Calcd. for Ci ₂ H ₁₀ FNO ₄ : 252.1 (M+H); found: 252.0. E. (3-(2-Fluoro-5-methoxyphenyl)isoxazol-5-yl)methanol, 7e

To a solution of compound 7d (1.5 g, 6.0 mmol) in THF (60 mL) was added LiAlH ₄ (272 mg, 7.17 mmol) in portions at 0 °C. The resulting solution was stirred for 20 mm at 0 °C. The reaction was then quenched by satd. sodium potassium tartrate solution (aq., 30 mL) and stirred vigorously for 30 min. The resulting solution was extracted with EtO Ac (3 x 50 mL). The organic layers were combined, dried over anhydrous sodium sulfate and concentrated. The residue obtained was purified by column chromatography on silica gel (EtO Ac/petroleum ether, 1 : 100-1 : 10) to give compound 7e as a yellow oil Mass Spectrum (LCMS, ESI pos.): Calcd. for i ^' _: , ! 1 _; „Γ\Ό·.· 224, 1 (M+H); found: 224,0.

F, (4-Bromo-3-(2-fluoro-5-methoxyphenyl)isoxazol-5-yl)methanol, 7f

A solution of compound 7e (950 mg, 4.26 mmol), NBS (1.14 g, 6.39 mmol), and PPTS (46 mg, 0.18 mmol) in DCM (20 mL) was stirred overnight at RT. The reaction was then quenched by water (20 mL). The resulting solution was extracted with DCM (3 x 15 mL). The organic layers were combined, dried over anhydrous sodium sulfate and concentrated. The residue obtained was purified by column chromatography on silica gel

(EtO Ac/petroleum ether, 1 : 100-1 : 10) to give compound 7f as a colorless oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for CnHgBrFNC : 302.0 (M+H); found: 301.9.

G. 4-Bromo-5-(chloromethyl)- -(2-fluoro-5-methoxyphenyl)isoxazole, 7g

To a stirring solution of compound 7f (300 mg, 0.990 mmol) in DCM (20 mL) and DMF (1 mL) was added thionyl chlonde (238 mg, 2.0 mmol) dropwise at 0 °C. The resulting solution was stirred for 1 h at RT. The reaction was then quenched by satd. NH ₄ CI (aq. 10 mL). The resulting solution was extracted with dichloromethane (3 x 10 mL). The combined organic layers were washed with H ₂ 0 (2 x 10 mL), dried over anhydrous sodium sulfate and concentrated to give compound 7g as a yellow oil. Mass Spectrum (LCMS, ESI pos.); Calcd. for CiiH ₈ BrClFN0 ₂ : 319.9 (M+H); found: 319.9.

H. (35)-3-Cyclopropyl-3-(3-((4-(5,5-dimethylcyclopent-l-enyl)-3 -(2-fluoro-5- methoxyphenyl)isoxazol-5-yl)methoxy)phenyl)propanoic acid, Cpd 62

The title compound 62 was prepared from compound 7g and methyl (35)-3-cyclopropyl-3- (3-hydroxyphenyl)propanoate (using an analogous procedure to that described in Example

I, Step F) and then using the methods described in Example 5, Steps F-H . Tl-NMR (300 MHz, DMSO-de) δ (ppm): 7.21 - 7.32 (m, 2H), 7.02 - 7.05 (m, 2H), 6.88 - 6.94 (m, 3H), 5.85 (s, 1H), 5.11 (s, 2H), 3.75 (s, 3H), 2,63 - 2.68 (m, 2H), 227 - 2.35 (m, 3H), 1.67 (t, J = 6.9 Hz, 2H), 0.90 - 1.05 (m, 1H), 0.67 (s, 6H), 0.44 - 0.54 (in, 1H), 0.21 - 0.40 (m, 211), 0.02 - 0.18(m, 1H). Mass Spectrum (LCMS, ESI pos.): Calcd. for C3oH ₃₂ FN0 ₅ : 504.2 (M H); found: 504.2.

Example 8

(35)-3-Cyclopropyl-3-(3-((l-(2-fluoro-5-methoxyphenyl)-5-iso butyl-lH-l,2,3-triazol-4- yl)methoxy)phenyl)propanoic acid (Cpd 83)

A. Ethyl 5-methyl-3-oxohexanoate, 8a

To a stirring solution of 4-methylpentan-2-one (5.0 g, 50 mmol) in THE (50 mL) was added sodium hydride (60 % in mineral oil, 4.0 g, 100 mmol) portionwise at 0 °C. After 20 mm, diethyl carbonate (8.0 g, 68 mmol) was added and the mixture was stirred for 2 h at 80 °C. The reaction mixture was allowed to cool to RT and treated with satd. NH ₄ CI (aq. 100 mL). The resulting mixture was extracted with EtOAc (2 x 100 mL). The organic layers were combined and concentrated under reduced pressure. The residue obtained was purified by column chromatography on silica gel (EtO Ac/petroleum ether, 1 : 100) to give compound 8a as a light yellow oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for C ₉ H ₁₆ O ₃ : 173.1 (M i l); found: 173, 1.

B. Ethyl l~(2-fluoro~5-methoxyphenyl)~5~isobutyl-lH~l,2,3 riazole-4-carboxylate, 8b

A solution of compound 8a (200 mg, 1.16 mmol), 2-azido-l-fluoro-4-methoxybenzene (210 mg, 1.26 mmol, as prepared in Example 5, Step A) and pyrrolidine (17 mg, 0.24 mmol) in DMSO (1 mL) was stirred overnight at 80 °C. The reaction mixture was allowed to cool to RT and treated with water (4 mL). The resulting solution was extracted with EtOAc (2 x 4 mL). The organic layers were combined and concentrated. The residue obtained was purified by column chromatography on silica gel (EtO Ac/petroleum ether, 1 :3) to give compound 8b as a colorless oil Mass Spectrum (LCMS, ESI pos.):Calcd. for C _! ,,! ! _. , ::F\ :()-,: 322.1 (M+H); found: 322, 1.

C. (l-(2-Fluoro-5-methoxyphenyl)-5-isobutyl-lH-l ,2,3-triazol-4-yl)methanol, 8c

To a solution of compound 8c (180 mg, 0.56 mmol) in THE (5 mL) was added DIBAL-H (1M in hexane, 2, 8 mL) at -30 °C. The mixture was stirred for 2 h at -30 °C. The reaction was then quenched by satd. sodium potassium tartrate solution (aq. 50 mL) and stirred vigorously for 30 min. The resulting solution was extracted with EtOAc (2 x 50 mL). The organic layers were combined and concentrated. The residue obtained was purified by column chromatography on silica gel (EtO Ac/petroleum ether, 1 : 1) to give compound 8c as a colorless oil. Mass Spectrum (LCMS, ESI pos.):Calcd. for C _M H _{I S} FN _S O _? .: 280.1 (M+H); found: 280. 1.

D. 4-(Chloromethyl)-l-(2-fluoro-5-methoxyphenyl)-5-isobutyl-lH- l,2,3-triazole, 8d

To a stirring solution of compound 8c (1 10 mg, 0.39 mmol) in DCM (5 mL) and DMF (0.1 mL) was added thionyl chloride (140 mg. 1.2 mmol) at 0 °C. The resulting solution was stirred for 1 h at 0 °C and concentrated to give compound 8d as a colorless oil.

E. (35)-Ethyl 3-cyclopropyl-3-(3-((l-(2-fluoro-5-methoxyphenyl)

-5-isobutyl-lH-l -triazol-4-yl)methoxy)phenyl)propanoate, 8e

A mixture of compound If (113 mg, 0.48 mmol), compound 8d (130 mg, 0.44 mmol) and Cs ₂ C0 (356 mg, 1.09 mmol) in C¾CN (8 mL) was stirred overnight at RT. The resulting mixture was concentrated. The residue was treated with water (20 mL) and extracted with EtOAc (2 x 20 mL). The organic layers were combined and concentrated. The residue obtained was purified by column chromatography on silica gel (EtO Ac/petroleum ether, 1 :5) to give compound 8e as a colorless oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for C28H34FN3O4: 496.3 (M+H); found: 496.3,

F. (35)-3-Cyclopropyl-3-(3-((l -(2-fluoro-5-methoxyphenyl)-5-isobutyl-lH-l ,2,3-tri azol-4-yl)methoxy)phenyl)propanoic acid, Cpd 83

Compound 83 was prepared from compound 8e following the procedure described in Example 3, Step I I Ί ΐ-W!R (400 MHz, DMSO-d ₆ ) δ (ppm): 7.51 (t, J ------- 9,2 Hz, 1 1 1).

7.18 - 7,32 (m, 3H), 6.85 - 6,92 (m, 3H), 5.16 (s, 2H), 3.81 (s, 3H), 2.59 - 2.61 (m, 2H), 2.31 - 2,48 (m, 3H), 1,60 - 1 .63 (m, ! ! !}. 0,95 - 0.96 (m, I I I). 0.72 (d, J ------ 6.4Hz, 6H), 0.43 - 0.45 (m, lH), 0.23 - 0.29 (in, 2H), 0.08- 0.09 (m, III). Mass Spectrum (LCMS, ESI pos.): Calcd. for C\j hJ- ^' VOj: 468.2 (M 1 1 ); found: 468.1.

Following the procedures described in Example 8 above, and substituting suitably selected and substituted reagents, starting materials and conditions as would be readily apparent to those skilled in the art, the following representative compound of the present invention was prepared.

Example 9

(35)-3-Cyclopropyl-3-(3-((4-(5,5-dimethylcyclopent-l-enyl)-5 -(2-fluoro-5- methoxyphenyl)isothiazoi-3-yl)methoxy)phenyl)propanoic acid (Cpd 30)

A. 4-Bromo-3-methylisothiazol-5-amine, 9a

To a solution of 3-inethyl-l ,2-thiazol-5-amine (2.00 g, 17.5 mniol) and DIEA (3.07 g, 23.7 mmol) in ACN (50 mL) was added NBS (2.35 g, 13.2 mmol). The resulting solution was stirred for 20 min at RT and treated wirh said, sodium bicarbonate (aq. 50 mL). The resulting solution was extracted with EtOAc (100 mL). The organic layer was concentrated and the residue obtained was purified by column chromatography on silica gel

(EtO Ac/petroleum ether, 1 :9) to give compound 9a as a brown solid. Mass spectrum (LCMS, ESI pos): Calcd. for C I UBrVS: 192.9 (M+H); found: 192.9. B. 4,5-Dibromo-3-methylisothiaz 9b

A mixture of CuBr ₂ (0.58 g, 2.6 mmol) and t-BuONO (0.48 g, 4.7 mmol) in ACN (20 mL) was stirred for 20 min at 0 °C. A solution of compound 9a (0.50 g, 2.6 mmol) in ACN (5 mL) was then added dropwise. The resulting solution was stirred for an additional 2 h at RT, treated with satd. NH ₄ CI solution (aq. 10 mL) and extracted with EtO Ac (30 mL).

The organic layer was concentrated and the residue obtained was purified on silica gel with EtO Ac/petroleum ether (1 : 100) to give compound 9b as a light yellow solid. 1H-NMR (300 MHz, CDCI ₃ ) δ (ppm): 2.56 (s, 3H). Mass spectrum (GCMS, EI): Calcd. for C ₄ H ₃ Br ₂ NS: 254.8 (M); found: 254.4, 256.4, 258,4. l-Bromo-5-(2-fluoro-5-methoxyphenyl)-3-methylisothiazole, 9c

A mixture of (2-fluoro-5-methoxyphenyl)boronic acid (345 mg, 2.03 mmol), Pd(dppf)Cl ₂ (79 mg, 0.96 mmol), sodium carbonate (509 mg, 4.80 mmol) and compound 9b (480 mg, 1.87 mmol) in 1,4-dioxane (2 mL) and water (0.5 mL) was stirred for 8 h at 60 °C under N ₂ . The reaction mixture was allowed to cool to RT, treated with satd. NH4CI solution (aq. 10 mL) and extracted with EtOAc (30 mL). The organic layer was concentrated. The residue obtained was purified by column chromatography on silica gel (EtOAe/petroleum ether, 1 : 100) to give compound 9c as a white solid. Mass spectrum (LCMS, ESI pos): Calcd. for C _n H ₉ BrFNQS: 302.0 (M H i. found: 301.9.

D. 4-Bromo-3-(brornomethyl -5-(2-fluoro-5-methoxyphenyl)isothiazole, 9d

A solution of NBS (213 mg, 1.20 mmol), AIBN (33.4 mg, 0.200 mmol), and compound 1 lc (300 mg, 0.990 mmol) in CCI4 (6 ml.) was stirred overnight at 80 °C under N ₂ . The reaction mixture was allowed to cool to RT and treated with satd. sodium bicarbonate (aq., 20 mL). The resulting solution was extracted with EtOAc (3 x 30 mL). The organic layers were combined and concentrated. The residue obtained was purified by column chromatography on silica gel (EtO Ac/petroleum ether, 1 : 100) to give compound 9d as a light yellow solid. Mass spectrum (LCMS, ESI pos): Calcd. for C _n ¾Br ₂ FNOS: 380.9 (M+H); found: 379.8, 381,8, 383.8.

E. (3«S)-Methyl 3-(3-((4-bromo-5-(2-fluoro-5-methoxyphenyl)isothiazol-3-yl)

methoxyjphenyl -3-cyclopropylpropanoate, 9e

A mixture of compound 9d (220 mg, 0.580 mmol), methyl (3 ^* )-3-cyclopropyl-3-(3- hydroxyphenyl)propanoate (120 mg, 0.54 mmol) and CS2CQ3 (352 mg, 1.08 mmol) in ACN (15 mL) was stirred for 6 h at 60 °C. The reaction mixture was allowed to cool to RT and then quenched by said. NH ₄ CI (aq., 10 mL). The resulting solution was extracted with EtO Ac (3 x 15 mL). The organic layers were combined and concentrated. The residue obtained was purified by column chromatography on silica gel (EtO Ac/petroleum ether, 95:5) to give compound 9e as a light yellow oil. Mass spectrum (LCMS, ESI pos): Calcd. for C ₂₄ I½BrFNQ ₄ S: 520.1 (M+H); found: 519.9.

F. (3«S)-Methyl 3-cyclopropyl-3-(3-((4-(5,5-dimethylcyclopent-l-enyl)

-5-(2-fluoro-5-methoxyphenyl)isothiazol-3-yl)methoxy)phenyl) propanoate, 9f

A mixture of Cs ₂ C0 ₃ (11.8 mg, 0.040 mmol), Pd(dppf)Cl ₂ (263 mg, 0.360 mmoi), compound lie (150 mg, 0.290 mmol), and 2-(5,5-dimethylcyclopent-l -en-l-yl)-4,4,5,5- tetramethyl-l,3,2-dioxaborolane (73.5 mg, 0.330 mmoi) in dioxane (2 mL) and water (1 mL) was stirred for 4 h at 90 °C under N ₂ . The mixture was allowed to cool to RT, treated with satd. NH ₄ CI (aq. , 10 mL). The resulting solution was extracted with EtO Ac (2 x 30 mL). The combined organic layers were concentrated and the residue obtained was purified by column chromatography on silica gel (EtO Ac/petroleum ether, 90: 10) to give compound 9f as a light yellow oil. Mass spectrum (LCMS, ESI pos): Calcd. for

C31H34FNO4S: 536.2 (M+H); found: 536.1.

G. (35)-3-Cyclopropyl-3-(3-((4-(5,5-dimethylcyclopent-l-enyl)-5 -(2-fluoro-5- methoxyphenyi)isothiazol-3~yi)methoxy)phenyl) ropanoic acid, Cpd 30

Compound 30 was prepared from compound 9f following the procedure described in Example 3, Step H. ^l H-NMR (300 MHz, DMSO-d ₆ ) δ (ppm): 7.12 - 7.33(m, 4H), 6.87 ·· 6.89 (m, 3H), 5.98 (s, 1H), 5.04 (s, 2H), 3.85 (s, 1H), 2.65 - 2.70 (m, 2H), 2.28 - 2.36 (m, 3H), 1.63 - 1.69 (m, 2H), 0.90-0.98 (m, IH), 0.49 ·· 0.86 (m, 7H), 0.24 - 0.26 (m, 2H), 0.01 - 0.12 ( m. 1 1 1 ). Mass Spectrum (LCMS, ESI pos.): Calcd. for C ₃ 0H32FNO4S: 522.2 (M+H); found: 522.1.

Example 10

(35)-3-(3-((5-(5-Cyano-2-fluorophenyl)-4-(5,5-dimethylcyclop ent-l-enyl)thiophen-3- yl)methoxy)phenyl)-3-cyclopropylpropanoic acid (Cpd 38)

-Bromothiophene-3-carboxylic acid, 10a

To a stirred solution of n-BuLi (2.5M in THE, 90.8 mL, 227 mmol) in THE (500 mL) at -78 °C was added 3,4-dibromothiophene (50.0 g, 207 mmol) dropwise under N ₂ . The resulting mixture was stirred for 30 min at -78 °C and treated with C0 ₂ (s) (250 g) and stirred for another 1 h at -78 °C. The reaction was then quenched by the addition of 1M sodium hydroxide solution (aq., 300 mL). After the mixture reached RT, the pH of the aqueous solution layer was adjusted to ~ 3 using 1 N hydrogen chloride solution. The solids formed were collected by filtration and dried to give compound 12a as a white solid. Mass Spectrum (LCMS, ESI neg.): Calcd. for C ₅ H ₃ Br0 ₂ S: 204.9 (M-H); found: 205.0. B. 4-Bromo-5-iodothiophene-3-carboxylic acid, 10b

A solution of compound 10a (36.0 g, 174 mmol) in AcOH (850 mL) was stirred for 30 min at 90 °C. To this was added a solution of sulfuric acid H ₂ S0 ₄ (3.5 mL) and H10 ₃ (7.55 g) in water (25 mL) at 90 °C. The reaction mixture was then treated with I ₂ (17.6 g, 69.3 mmol) in several hatches, stirred for an additional 3 h at 90 °C and then at RT overnight. The reaction mixture was cooled in an ice bath and the solids formed were collected by filtration and washed with hexane (3 x 100 mL) to give compound 12b as a white solid. Mass Spectrum (LCMS, ESI neg.): Calcd. for Of Hir!O^S: 330.8 (M-H); found: 330.8,

332.8.

C. (4-Bromo-5-iodothiophen-3-yl)methanol, 10c

To a stirred solution of compound 10b (12 g, 36 mmol) in THF (200 mL) was added a solution of BH ₃ (1 M in THE, 72 mL) dropwise at -30 °C under N ₂ . The resulting solution was stirred for 30 min at -30 °C and an additional 2 h at RT. The reaction was then cooled in an ice-water bath and quenched by slow addition of methanol (200 mL) followed by water (200 mL). The resulting solution was extracted with EtO Ac (300 mL). The organic layer was concentrated. The resulting residue was purified by flash chromatography (EtO Ac/petroleum ether 3:7) on silica gel to yield compound 12c as a white solid). Mass Spectrum (LCMS, ESI pos.): Calcd. for C ₅ H ₄ BrIOS: 300.8 (M-OH); found: 301.0,

3-Bromo-4-(chloromethyl)-2-iodothiophene,

no Compound 10c! was prepared from compound 10c following a similar procedure as described in Example 5, Step E. 1H-NMR (300 MHz, CDC1 ₃ ) δ (ppm): 7.57 (s, 1H), 4. (s, 2H).

E. (5)-Methyl 3-(3-((4-bromo-5-iodothiophen-3-yl)methoxy)phenyl)-3-cyclo

propylpropanoate, lOe

The title compound was prepared from compound lOd and methyl (3<S)-3-cyclopropyl-3- (3-hydroxyphenyl)propanoate following a similar procedure to that described in Example 5, Step !·. Mass Spectrum (LCMS, ESI pos.): Calcd. for CigH _¾8 BrI0 ₃ S: 520.9 (M+H); found: 520.8.

F. (3<S)-Methyl 3-(3-((4-bromo-5-(5-cyano-2-fluorophenyl)thiophen-3- yl)methoxy)phenyl)-3-cyclopropylpropano lOf

A mixture of compound lOe (120 mg, 0.23 mmol), (5-cyano-2-fluorophenyl)boronic acid (45 mg, 0.27 mmol), XPhos (9 mg, 0.01 mmol), K ₃ PO4 (97 mg, 0.46 mmol) m THF (0.46 mL) was stirred for 3 h at 50 °C under N ₂ . The reaction mixture was allowed to cool to RT and treated with H ₂ 0 (10 mL). The resulting mixture was extracted with EtOAc (2 x 10 mL). The organic layers were combined, dried over anhydrous sodium sulfate and concentrated. The resulting residue was purified by flash chromatography (0-30 % EtO Ac/petroleum ether) on sihca gel to obtain compound 1 Of as a colorless oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for C25H21B1-FNQ ₃ S: 514.0 (M+H); found: 513.9.

G. (35)-Methyl 3-(3-((5-(5-cyano-2-fluorophenyl)-4-(5,5-dimethylcyclopent-l - enyl)thiophen-3- l)methoxy)phenyl)-3-cyclopropylpropanoate, lOg

The title compound was prepared from compound l Of and 2-(5,5-dimethylcyclopent-l-en- 1 -yl)-4,4,5,5-tetramethyl-l ,3,2-dioxaborolane following a procedure similar to that described in Example 5, Step G. Mass Spectrum (LCMS, ESI pos.): Calcd. for

C32H32FNO3S: 530.2 (M-i-H); found: 530.0

H. (3S)-3-(3-((5-(5-Cyano-2-fluorophenyl)-4-(5,5-dimethylcyclop ent-l-enyl)thiophen-

3-yl)methoxy)phenyl)-3-cyclopropylpropanoic acid, Cpd 99

Compound 38 was prepared from compound lOg following a procedure similar to the experimental described in Example 1, Step H. ^X H-NMR (300 MHz, DMSO-d ₆ ) δ (ppm): 1 1.76 (br, 1H), 7.74 - 7.76 (m, 2H), 7.62 (s, 1 1 1 ). 7.32 (t, ,/ 9.0 Hz, 1H), 6.95 - 7.00 (m, 1H), 6.57 - 6.64 (m, 3H), 5.67 (s, 1H), 4.65 (s, 2H), 2.37 - 2.42 (m, 2H), 2.05 - 2.07 (in, 3H), 1.31 ·· 1.48 (m, 2H), 0.70 - 0.85 (m, IH), 0.00 ·· 0.65 (m, 10H). Mass Spectrum (LCMS, ESI neg.): Calcd. for C ₃₁ H ₃₀ FNO ₃ S: 514.2 (M-H), found: 514.1 .

Following the procedures described in Example 10, and substituting suitably selected reagents, starting materials and conditions as would be readily apparent to those skilled in the art, the following representative compound of the present invention were prepared. Cpd No. Characterization

(3iS ¹ )-3-Cyclopropyl-3-(3-((4-(5,5-dimethylcyclopent-l-enyl )-5- (2-fluoro-5-methoxyphenyl)thiophen-3-yl)methoxy)phenyl) propanoic acid

¹ H-NMR: (300 MHz, CD ₃ OD) δ (ppm) : 7.14 - 7,20 (rn, 1H), 7.02 - 7.06 (m, 2H), 6.79 - 7.00 (m, 5H), 5.42 (s, 1H), 5.25 (s,

13 2H), 3.77 (s, 3H), 2.50 - 2.70 (m, 2H), 2.30 - 2.45 (m, IH), 2.20 - 2.30 (m, 2H), 1.74 (t, J= 6.9 Hz, 2H), 1.03 (s, 6H), 0.90 - 1.00 (m, IH), 0.50 - 0.60 (m, IH), 0.25 - 0.40 (m, 2H), 0.00 - 0.15 (m, IH).

Mass Spectrum (LCMS, ESI pos.): Calcd. for C31H32FO4S: 519.2 (M+H); found: 519.1,

(5)-3-Cyclopropyl-3-(3-((3-(5,5-dimethylcyclopent-l-enyl)-2, 3'- bithiophen-4-yl)methoxy)phenyl)propanoic acid

1H-NMR(400 MHz, DMSO-de) δ (ppm): 7.48 - 7.52(m, 3H), 7.18 - 7.24 (m, 2H), 6.79 - 6.84 (m, 3H), 5.79 - 5.81 (m, IH),

16 4.87 (s, 2H), 2.59 - 2.64 (m, 3H), 2.35 - 2.39 (m, 3H), 1.69 - 1.74 (m, 2H), 1.05 -1.08 (m, IH), 0.72 (s, 6H), 0.00 - 0.60 (m, 4H).

Mass Spectrum (LCMS, ESI pos.): Calcd. for (^! I-^O-.S-.: 478.2 (M+H); found: 478.2.

Cpd No. Characterization

(S)-3-cyclopropyl-3-(3-((5-(3-(dimethylamino)phenyl)-4-(5,5- dimethy lcyclopent- 1 -en- 1 -yl)thi ophen-3 - yl)methoxy)phenyl)propanoic acid

¹ H-NM (400 MHz, DMSO-i ₆ ) δ (ppm): 7.50 (s, IH), 7.13 - 7.22 (m, 2H), 6.92 (s, I H), 6.78 - 6.85 (m, 4H), 6.67 - 6.71 (m,

17 1 H), 5.83 (s, IH), 4.88 (s, 2H), 2.88 (s, 6H), 2.50 - 2,62 (m, 2H), 2.36 - 2.49 n. 3H), 1.68 (t, ./ 6.9 Hz, 2H), 0.95 - 1.03 (m, IH), 0.68 (s, 6H), 0.49 - 053 (m, IH), 0.32 - 0.36 (m, IH), 0.22 - 0.26 (m, IH), 0.08 - 0.12 (m, IH).

Mass Spectrum (LCMS, ESI pos. ): Calcd. for C ₃₂ H ₃₇ NO ₃ S: 516.2 (M+H); found: 516.1

(S)-3-cyclopropyl-3-(3-((4-(5,5-dimethylcyclopent- -en- 1 -yl)-5- (2-fluoro-4-methoxyphenyl)thiophen-3- yl)methoxy)phenyl)propanoic acid

1H-NMR (400 MHz, OM$Q-d ₆ ): 6 (ppm): 7,69 (s, I H), 7.34 (t, J = 8.7 Hz, IH), 7.15 (t, J = 7.8 Hz, IH), 6.74 - 6.92 (m, 5H), 5.78

18

(s, IH), 4.85 (s, 21 1 ). 3.79 (s, 3H), 2.27 - 2.50 (111, 5H), 1 ,55 - 1.65 (m, 2H), 0.53 - 0.93 (m, 7H), 0.35 - 0.50 (111, H), 0,20 - 0.32 (m, 2H), 0,00 - 0, 5 (m, IH).

Mass Spectrum (LCMS, ESI pos,): Calcd, for C ₃₁ H ₃₃ FO ₄ S: 519.2 (M+H); found: 519.1 ,

Cpd No. Characterization

( _i S ^' )-3-Cyclopropyl-3-(3-((4-(5,5-dimethylcyclopent-l-enyl )-5-(3- methoxyphenyl)thiophen-3-yl)methoxy)phenyl)propanoic acid 1H-NMR (300 MHz. DMSO-de) δ (ppm): 7.40 (s, 1 H), 7.01 - 7.07 (m, 2H), 6,85 - 6.86 (m, 2H), 6.57 - 6.66 (m, 4H), 5.66 (s,

26 I H), 4.62 (s, 2H), 3.51 (s, 3H), 2.64 - 2,66 (m, 2Ή), 2.27 - 2.45 (m, 3B), 1.42 (s, 2H), 0,81 - 0.97 (m, 4H), 0.15 - 0.80 (m, 6H), 0.10 (s, 1H).

Mass Spectrum (LCMS, ESI pos.): Calcd. for C30H33NO4S: 503.2 (M+H); found: 504.0.

(»S -3-Cyclopropyl-3-(3-((4-(5,5-dimethylcyclopent-l-enyl)-5-(5- methoxypyridin-3-yl)thiophen-3-yl)methoxy)phenyl)propanoic acid

1H-NMR (300 MHz, DMSO-d ₆ ) δ (ppm): 1 1.8 - 12.1 (m, I I I). 8.21 - 8,34 (dd, 2H), 7,70 - 7.75 (s, 1 H), 7.45 - 5.49 (m, 1 H),

28 7.18 - 7.29 (t, 1H), 6.78 - 6.91 (m, 3H), 5.92 - 5.97 (s, I B), 4,88 - 4,93 is. 2H), 3.81 - 3.88 (s, 3H), 2.61 - 2.69 (m, 2H), 2.31 - 2.42 (in, 2H), 2.20 - 2.30 (m, l H), 1.60 - 1 ,75 (s, 1 H), 0.75 - 1 .05 (m, 5H), 0, 14 - 0.52 (m, 5H), 0.08 - 0, 1 1 (m, 1H). Mass Spectrum (LCMS, ESI pos,): Calcd, for C30H33NO4S: 504.2 (M+H); found: 504.0.

Cpd No. Characterization

(S)-3-cyclopropyl-3-(3-((4-(5,5-dimethylcyclopent-l-en-l-yl) -5- (2-methoxypyridin-4-yl)thiophen-3- yl)methoxy)phenyl)propanoic acid

1H-NMR (400 MHz. DMSO-i¾): δ (ppm): 8.1 6 (d, ./ = 5.4 Hz,

1H), 7.23 ( i. J 7.8 Hz, 1H), 7.09 - 7.13 i n) . 2H), 6.87 - 6.96 (m, 4H),

31

5.60 (t, J = 2.1 Hz, 1 H), 5.31 (s, 2H), 3.87 (s, 3H), 2.65 - 2.69 (m, 2H), 2.26 - 2.35 (m, 3H), 1 .80 (t, J = 6.9 Hz, 2H), 0.99 - 1.03 (m, 7H), 0.50 - 0.52 (m, 1 H), 0.24 - 0,34 (m, 2H), 0.12 - 0.15 (m, 1H).

Mass Spectrum (LCMS, ESI pos.): Calcd. for C ₃ H ₃₃ NO ₄ S:

502.2 (M-H); found: 502,0.

Example 1 1

(35)-3-Cyclopropyl-3-(3-((4-(5,5-dimethylcyclopent-l-enyl)-5 -(5-fluoro-2- methoxypyridin-4-yl)thiophen-3-yl)methoxy)phenyl)propanoic acid (Cpd 27)

A. Methyl 4-bromo-5-iodothiophene-3-carboxylate, 11a

To a stirring solution of compound 10b (2.75 g, 8.26 mmol) in methanol (100 mL) was added thionyl chloride (1 .97 g, 16.6 mmol), and the mixture was stirred for 2 h at 60 °C. The reaction was allowed to cool to RT and concentrated. The residue was dissolved in EtOAc (50 mL) and washed with satd. sodium bicarbonate (aq., 3 xlO mL). The organic layer was separated, dried over sodium sulfate and concentrated to give compound 11a as white solid. 1H-NMR (400 MHz, CDCI ₃ ) δ (ppm): 8.29 (s, i l l ). 3.89 (s, 3 H i. B. Methyl 4-bromo-5-(5-fluoro-2-methoxypyridin-4-yl)thiophene-3-carbox ylate, lib

A mixture of compound 1.1. a (600 mg, 1.73 mmol), (5-fluoro-2-methoxypyridin-4-yl) boronic acid (360 mg, 2.1 1 mmol), XPhos amraobiphenyl palladium chloride precatalyst (64 mg, 0.08 mmol) and 2M K ₃ PO ₄ (aq., 1.7 mL, 3.4 mmol) in THF (6 ml.) was stirred overnight at 40 °C under N ₂ . The resulting mixture was allowed to cool to RT and concentrated. The residue obtained was purified by column chromatography on silica gel (EtO Ac/petroleum ether, 1:20) to give compound lib as a colorless oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for C , .| l.,Br! ^; NO-.S: 345.9 (M+H); found: 345.9,

C. (35)-3-Cyclopropyl-3~(3-((4-(5,5-dimethylcyclopent-l-enyl)-5 -(5-fluoro-2- methoxypyridin-4-yl)thiophen-3-yl)methoxy)phenyl)propanoic acid, Cpd 27 Compound 27 was prepared from compound l i b and 2-(5,5-dimethykyclopent-l -en-l-y])- 4,4,5,5-tetramethyl-l,3,2-dioxaborolane following the procedure described in Example 3, Steps D-H. 'H- MR (300 MHz, D SO-de) δ (ppm): 8.22 (s, I H), 7,89 (s, IH), 7, 17 (t, J = 7.8 Hz, I H), 6,94 (d, J ------- 4.8 Hz, IH), 6,77 - 6.85 (m, 3H), 5.88 (s, IH), 4.87 (s, 2H),

3.84 (s, 3H), 2.56 - 2.58 (m, 2H), 2,28 - 2.36 (m, 3H), 1 ,62 - 1.66 (m, 2H), 0.38 - 1 ,00 (m, 8H), 0.14 - 0.32 (m, 2H), 0,07 - 0.10 (m, I H), Mass Spectrum (LCMS, ESI pos.): Calcd. for C30H32FNO4S: 522.2 (M+H); found: 522.3.

Example 12

(36)-3-Cyclopropyl-3-(3-((4-(5,5-dimethylcyclopent-l -enyl)-5-(6- methoxybenzo[b]thioph -4-yl)thiophen-3-yl)methoxy)phenyl)propanoic acid (Cpd 10)

-Bromo-6-methoxybenzo[b]thiophene-2-carboxylic acid, 12a

A solution of methyl 4-bromo-6-methoxy-l-benzothiophene-2-carboxylate (22 g, 73 mmol) and sodium hydroxide (29 g, 73 mmoi) in water (122 mL) and THF (400 mL) was stirred for 12 h at RT. The pH of the solution was adjusted to 4-5 with IN hydrogen chloride solution. The resulting solution was extracted with EtOAc (3 x 200 mL). The organic layers were combined, dried over anhydrous sodium sulfate and concentrated under reduced pressure. The crude product obtained was recrystaliized from hexane to obtain compound 12a as a yellow solid. 1H-NMR (300 MHz, DMSO-d ₆ ) δ (ppm): 7.86 (s, 1H), 7.70 (s, 1H), 7.38 (s, H I). 3.86 (s, 3H).

-Bromo-6-methoxybenzo[b]thiophene, 12b

A solution of compound 12a (4.0 g, 14 mmol) and DBU (12 g, 79 mmol) in DMA (20 mL) was heated at 200 °C in a microwave reactor for 40 min. The reaction mixture was treated with water (300 mL) and the pH value of the solution was adjusted to 6-7 with IN hydrogen chloride solution. The resulting mixture was extracted with EtOAc (3 x 200 mL). The organic layers were combined, dried over anhydrous sodium sulfate and concentrated. The residue was purified by column chromatography on silica gel

(EtO Ac/petroleum ether, 1 : 100-1 :30) to give compound 12b as a yellow oil. Mass Spectrum (GCMS, EI): Caicd. for C ₉ H ₇ BrOS: 241.9 (M): found: 242.0, 244.0.

C. 2-(6-Methoxybenzo[b]thiop ethyl-l,3,2-dioxaborolane, 12c

A mixture of compound 12b (600 mg, 2.47 mmol), B ₂ Pin ₂ (940 mg, 3.70 mmol),

Pd(dppf)Cl ₂ (90 mg, 0.12 mmol) and KOAc (726 mg, 7.40 mmol) in DMSO (5 mL) was stirred overnight at 80 °C under N ₂ . The reaction mixture was allowed to cool to RT, treated with water (20 mL) and extracted with EtO Ac (3 x 20 mL). The organic layers were combined, dried over anhydrous sodium sulfate and concentrated. The residue obtained was purified by column chromatography on silica gel (EtO Ac/petroleum ether, 1 : 100-1 :50) to give compound 12c as a green solid. 1H-NMR (300 MHz, CDC3 ₃ ) δ (ppm): 7.89 (d, 1 5.4 Hz, I H), 7,50 (d, ,/ 2.4 Hz, 1 H), 7,46 (d, J ------- 2.4 Hz, 1H), 7.30 (d, J ------ 5.7

Hz, I H), 3,89 (s, 3H), 1 ,39 (s, 12H),

D. (35)-Methyl 3-(3-((4-bromo-5-(6-methoxybenzo[b]thiophen-4-yl)thiopl

yl)methoxy)phenyl)-3-cyclopropylpropanoate, 12d

A mixture of compound lOe (400 mg, 0.770 mmol, as prepared in Example 10, Step E), compound 12c (245 mg, 0.840 mmol), Pd(dppf)Cl ₂ (28 mg, 0.040 mmol, ) and NaiCOr, (203 nig, 1.92 mmol) in dioxane (8 niL) and water (2 niL) was stirred for 2 h at 50 °C under N ₂ . The reaction mixture was allowed to cool to RT and treated with water (20 rnL). The resulting solution was extracted with EtOAc (3 x 15 rnL). The organic layers were combined, dried over anhydrous sodium sulfate and concentrated. The residue obtained was purified by column chromatography on silica gel (EtO Ac/petroleum ether, 1 : 100-1 :40) to give compound 12d as a colorless oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for C27H25B1-G4S2: 557.0 ( M H i. found: 557.2.

(35)-Methyl 3-cyclopropyl-3-(3-((4-(5,5-dimethylcyclopent-l-enyl)-5-(6- methoxybenzo[b]thiophen-4-yl)thiophen-3-yl)methoxy)phenyl)pr opanoate, 12e

Compound 12e was prepared from compound 12d and 2-(5,5-dimethylcyclopent-l-en-l- yl)-4,4,5,5-tetramethyl-l,3,2-dioxaborolane following a similar procedure to that described in Example 5, Step G. Mass Spectrum (LCMS, ESI pos.): Calcd. for C34H36Q S .: 573.2 (M+H); found: 573.3.

F. (35)-3-cyclopropyl-3-(3-((4-(5,5-dimethylcyclopent-l -enyl)-5-(6- methoxybenzo[b]thiophen-4-yl)thiophen-3-yl)methoxy)phenyl)pr opanoic acid, Cpd 10

Compound 10 was prepared from compound 12e, following a similar procedure to that described in Example 4, Step I. 1H-NMR (300 MHz, DMSO-de) δ (ppm): 1 1 .99 (s, 1H), 7.76 (s, ! FI), 7.55 - 7.59 (m, 2H), 7,36 - 7.39 (m, 1 H), 7,20 - 7.22 (m, 1H), 7.01 (d, J = 2.4 Hz, 1 H), 6,88 - 6.89 (m, 3H), 5,89 (s, 1 H), 4,90 (s, 2H), 3.81 (s, 3H), 2.68 - 2,70 (m, 2H), 2.25 - 2.27 (m, 3H), 1 ,44 - 1 .62 (m, 2H), 1 ,30 - 1.40 (m, 1 H), 0.00 - 1.10 (m, 10H). Mass Spectrum (LCMS, ESI pos.): Calcd. for C33H34O4S2: 557,2 [M-H]; found: 557. 1. Following the procedures described in Example 12 above, and substituting suitably selected reagents, starting materials and conditions as would be readily apparent to those skilled in the art, the following representative compound of the present invention were prepared.

Cpd No. Characterization

(3S)-3-cyclopropyl-3-(3-((4-(5,5-dimethylcyclopent-l-enyl)-5 - (2-fluorophenyl)thiophen-3-yl)methoxy)phenyl)propanoic acid ¾-NMR (300 MHz, DMSO-d ₆ ) δ (ppm): 11,89 (s, IH), 7.76 (s, IH), 7.39 - 7.45 (m, 2H), 7.20 - 7.22 (m, 3H), 6.83 - 6.87 (in, 3H), 5.81 (s,

21

IH), 4.87 i s. 211 ). 2.64-2.66 (m, 2H), 2.27-2.29 (in, 311 ). 1.65 (s, 211 ). 1.13 (s, IH), 0.15 - 0.90 (m, 9H), 0.10 (s, IH). Mass Spectrum (LCMS, ESI pos.): Calcd. for C30H31FO3S: 489.6 | M- i | ^' : found: 489.0.

(3S)-3-Cyclopropyl-3-(3-((4-(5,5-dimethy]cyclopent-l-enyl)-5 -

(2-methoxypheny])thiophen-3-yl)methoxy)phenyl)propar!oic acid

1H-NMR (300 MHz. DMSO-de) δ (ppm): 11.89 (s, I H) , 7.76 (s,

22 IH) , 7.39 - 7.45 (m, 2H) , 7.20 - 7.22 (m, 3H) , 6.83 - 6.87 (m, 3H), 5.81 (s, IH), 4.87 (s, 2H), 2.64 - 2.66 (m, 2H), 2.27 - 2.29 (m, 3H), 1.65 (s, 2H), 1.13 (s, IH), 0.15 - 0.90 (m, 9H), 0.10 (s, IH). Mass Spectrum (LCMS, ESI pos.): Calcd. for C31H34O4S: 501.6 [M-l] ^" ; found: 501.0.

(S)-3~Cyclopropy]-3-(3-((5-(3,5-dimethoxyphenyl)~4~(5,5- dimethylcyclopent- 1 -enyl)thiophen-3 - yl)methoxy)phenyl)propanoic acid

Ή-Ν Κ (300 MHz, DMSO-d ₆ ) δ (ppm): 11.98 (s, IH), 7.63 (s, IH), 7.18 - 7.23 (m, I H), 6.82 - 6.87 (m, 3H), 6.68 - 6.80 (m,

23

2H), 6.45 - 6.47 (m, IH), 5.89 (s, IH), 4.85 (s, 2H), 3.73 (s, 6H), 2.63 - 2.66 (m, 2H), 2.49 - 2,51 (m, 3H), 1.69 (s, 2H), 0.85 (s, 1 H), 0.42 - 0.45 (m, 6H), 0.22 - 0,32 (m, 2H), 0.11 - 0, 13 (d, J 4.8 Hz, I H). Mass Spectrum (LCMS, ESI pos.): Calcd, for C ₃ 2H36O5S: 531.1 [M-l] ^" ; found: 531.1. Cpd No. Characterization

(3S)-3-Cyclopropyl-3-(3-((4-(5,5-dimethylcyclopent-l-enyl)-5 - (l.H-indol-4-yl)thiophen-3-yl)methoxy)phenyl)propanoic acid

1H-NMR (300 MHz. DMSO-d ₆ ) δ (ppm): 11.22 (br, l i s). 7.66 (s, 1 H), 7.34 - 7.37 (m, 2H), 7.18 - 7,23 (m, 1H), 7.05 - 7,09 (m,

24 2H), 6.80 - 6.89 (m, 3H), 6.56 - 6.57 (m, 1 H), 5.85 - 5.86 (m,

1 H), 4.89 (s, 2H), 2.51 - 2,61 (m, 2H), 2.27 - 2,31 (m, 3H), 1.52 - 1.55 (m, 2H), 0.15 - 1.00 (m, 10H), 0.05 - 0.12 (m, III). Mass Spectrum (LCMS, ESI pos. ): Calcd. for C32H3 ₃ NO3S: 512.2 (M 1 1): found: 512.2.

(3,S -3-(3-((5-(Benzofuran-4-yl)-4-(5,5-dimethylcyclopent-l- enyl)thiophen-3-yl)methoxy)phenyl)-3-cyclopropylpropanoic acid

1H-NMR (300 MHz, DMSO-d ₆ ) δ (ppm): 8.03 (s, H i). 7.77 (s,

25 1 1 1). 7.64 (d, J ------- 8.7 Hz, i l l ). 7.39 (d, ./ 6.9 Hz, 1H), 7.19 -

7.27 (rn, 2H), 7.01 (s, I H), 6.82 - 6.90 (m, 3H), 5.89 (s, 1 H),

4.90 (s, 2H), 2.64 - 2.67 (m, 2H), 2.26 - 2.31 (m, 3H), 1.45 - 1.61 (rn, 2H), 0.12 - 1.11 (m, 1 I II). Mass Spectrum (LCMS,

ESI pos.): Calcd. for C32H32O4S: 512,7 (M+H); found: 512,7.

Example 13

^-(3-((4-Cyclobutoxy-5-(2-fluoro-5-methoxyphenyl)isoxazol-3- yl)methox\')phenyl)- 3-cyclopropylpropanoic acid (Cpd 43)

Ethyl 4-chloro-2-(hydroxyimino)-3-oxobutanoate, 13a 13a

To a stirring solution of ethyl 4-chloro-3-oxobutanoate (44.0 g, 267 mmol), AcOH (250 mL) was added a solution of NaN0 ₂ (23.2 g, 336 mmol) in water (200 niL) dropwise at - 10 °C. The resulting solution was stirred for 2 h at -4 °C. The reaction mixture was then treated with water (50 mL) and extracted with EtOAc (3 x 30 raL). The organic layers were combined and washed with I O (3 x 30 mL). The organic layer was dried over Na ₂ S0 ₄ and concentrated to give compound 13a as a colorless oil. ^X H-NMR (400 MHz,

CDCI ₃ ) δ (ppm): 4.59 (s, 2H), 4.41 (dd, Jj ------ 6.8 Hz, J ₂

3H).

B. Ethyl 4-hydroxyisoxazole-3-carboxylate, 13b

A mixture of compound 1.3a (44.0 g, 227 mmol) and urea (1 10 g, 1 .83 mol) in DMF (200 mL) was stirred for 20 min at 100 °C. The reaction mixture was allowed to cool to RT. The reaction was then quenched with water (50 mL). The resulting solution was extracted with DCM (3 x 20 mL). The organic layers were combined, washed with H ₂ 0 (2 x 5 mL) and concentrated. The residue obtained was purified by column chromatography (0-20% EtO Ac/petroleum ether) on silica gel to obtain compound 13b as a white solid. Mass Spectrum (LCMS, ESI pos.): Calcd. for Gj !-XO;: 158.0 ( M i l ): found: 158.0,

C. Ethyl 4-cyclobutoxyisoxazole-3-carboxylate, 13c

A mixture of compound 13b (500 mg, 3.18 mmol), bromocyclobutane (512 mg, 3.79 mmol) and CS2CO3 (2.08 g, 6.38 mmol) in DMF (5 mL) was stirred for 1 h at 60 °C. The reaction mixture was allowed to cool to RT and treated with water (20 mL). The resulting solution was extracted with DCM (3 x 20 mL). The organic layers combined, dried over Na ₂ S0 ₄ and concentrated. The residue obtained was purified by flash chromatography (0- 20% EtO Ac/petroleum ether) on silica gel to obtain compound 13c as a yellow solid. Mass Spectrum (LCMS, ESI pos.): Calcd. for C10H13FNO4: 212.1 (M+H); found: 212. 1. -bromo-4-cyclobutoxyisoxazole-3-carboxylate, 13c!

A solution of compound 13c (260 mg, 1.23 mmol) and NBS (262 mg, 1.47 mmol) in DMF (2 mL) was stirred overnight at 60 °C. The reaction mixture was allowed to cool to RT. The reaction mixture was treated with water (10 mL) and extracted with DCM (3 xlO mL). The separated organic layers were combined, dried over Na ₂ S0 ₄ and concentrated. The residue obtained was purified by flash chromatography (0-25% EtO Ac/petroleum ether) on sihca gel to obtain compound 13d as a yellow solid. Mass Spectrum (LCMS, ESI pos.): Calcd. for C i.,1 1 ; -.BrNOj: 290.0 (M+H); found: 290.0.

E. Ethyl 4-cyclobutoxy-5- -fluoro-5-memoxyphenyl)isoxazole-3-carboxylate, 13e

A mixture of compound 13d (293 mg, 1.01 mmol), (2-fluoro- 5 -methoxy phenyl ) boronic acid (250 mg, 1.47 mmol), Pd(dppf)Cl ₂ (31.6 mg, 0.04 mmol) and Cs ₂ C0 ₃ (562 mg, 1.72 mmol) in dioxane (0.5 mL) and water (2 mL) was stirred for 1 h at 60 °C under N ₂ . The reaction mixture was allowed to cool to RT. The reaction was then quenched with said. NH ₄ CI (aq. 5 mL). The resulting solution was extracted with EtO Ac (3 x 5 mL) and the organic layers were combined, washed with H ₂ 0 (2 x 10 mL), dried over Na ₂ S0 ₄ and concentrated. The residue obtained was purified by flash chromatography (0-20% EtO Ac/petroleum ether) on silica gel to obtain compound 13e as a yellow solid. Mass Spectrum (LCMS, ESI pos.): Calcd. for C V ! ί ι,,ί-ΛΟ,: 336.1 (M+H); found: 336.1.

F. (4-Cyclobutoxy-5-(2-fluoro-5-methoxyphenyl)isoxazol-3-yl)met hanol, 13f

To the stirring solution of compound 13e (160 mg, 0.478 mmol) in THF (4 mL) was added the solution of DIBAL-H in THF (1 M, 2.5 mL, 2.5 mmol) was added at -78 °C. The resulting solution was stirred for 1 h at -78 °C and the reaction was then quenched with satd. seignette salt solution (aq. 5 mL). The resulting solution was extracted with EtO Ac (3 x 5 mL). The organic layers were combined, washed with water (2 x 10 mL) and concentrated. The residue obtained was purified by flash chromatography (0-20%

EtO Ac/petroleum ether) on silica gel to obtain compound 13f as colorless oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for C ₁₅ H ₁₆ FN0 ₄ : 294.1 (M+H); found: 294.2.

G. (35)-methyl 3-(3-((4-cyclobutoxy-5-(2-fluoro-5-methoxyphenyl)isoxazol-3- yi)methoxy)phenyl)-3-cyciopropylpropanoate, 13g

Compound 13g was prepared from compound 13f followmg the procedures described in Example 14, Steps E and F. Mass Spectrum (LCMS, ESI pos.): Calcd. for C ₂ gH ₃ oFN() ₆ : 496.2; found: 496.1.

H. (35)-3-(3-((4-Cyclobutoxy-5-(2-fluoro-5-methoxyphenyl)isoxaz ol-3- yl)methoxy)phenyl)-3-cyclopropylpropanoic acid, Cpd 43

Compound 43 was prepared from compound 13g following the procedure described in Example 4, Step I. 1H-NMR (300 MHz, DMSO-d ₆ ) δ (ppm): 12.00 (brs, IH), 7.22 - 7.40 (m, 3H), 7.13 - 7.18 (m, IH), 6.89 - 6.97 (m, 3H), 5.19 (s, 2H), 4.48 - 4.53 (m, IH), 3.81 (s, 31 1). 2.64 - 2.68 (m, 2H), 2.26 - 2.29 (m, IH), 1 ,94 - 2.11 (m, 4H), 1 ,59 - 1.62 (m, IH),

I .35 - 1,38 (nr. IH), 0.95 - 1.05 (m, IH), 0.47 - 0.51 (m, i l l). 0.24 - 0.29 (m, 2H), 0.12 - 0.14 (m, IH), Mass Spectrum (LCMS, ESI pos,): Calcd, for ( y! I ·;. ^■ ] \( ^" },,: 482.2 (M+H); found: 482.2.

Following the procedures described in Example 13 above, and substituting suitably selected reagents, starting materials and conditions as would be readily apparent to those skilled in the art, the following representative compound of the present invention were prepared.

Cpd No. Characterization

(3S)-3-(3-[[4-(Cyclopenty1oxy)-5-(2-fluoro-5- methoxyphenyl)isoxazol-3-yl]methoxy]phenyl)-3- cyclopropylpropanoic acid

1H-NMR (400 MHz, DMSO-d ₆ ) δ (ppm): 7,34 - 7.39 (m, I H), 7.28 - 7.29 (m, 1 H) ,7.20 - 7.23 (m, IH), 7. 13 - 7.16 (ra, I II), 6.92 - 6,96 (in, IH), 6.88 - 6.91 (m, 2H), 5.19 (s, 2H), 4.61 (s,

1

1H), 3.80 (s, 3H) , 2.50 - 2.59 (m, 2H), 2.28 - 2.33 (m, IH), 1.69 (m, 2H) , 1.56 - 1.61 (m, 4H), 1.47 (m, 2H), 1.23 (m, IH), 0.97 - 0.99 (m, IH), 0.47 - 0.48 (m, 2H), 0.29 - 0.30 (m, IH) , 0.03 - 0.13 (m, I H).

Mass Spectrum (LCMS, ESI pos.): Calcd. for

496.2 (M+H); found: 496.2.

(3S)-3-cyclopropyl-3-(3-((5-(2-fluoro-5-methoxyphenyl)-4- i sobutoxy i soxazol-3 -yl)methoxy)phenyl)propanoi c ac d ^l-NMR (300 MHz, DMSO-de) δ (ppm): 7.11 - 7.40 (m, 4H), 6.87 - 6.96 (m, 3H), 5.22 (s, 2H), 3.80 (s, 3H), 3.74 - 3.76 (d. ./

39 6 Hz, 2H), 2.57 - 2.61 (m, 2H), 2.28 - 2.34 (m, IH), 1 ,84 - 1.91 (m, IH), 0.94 - 0.99 (m, IH), 0.84 - 0.86 (d, 6 Hz, 6H), 0.47 - 0,51 (m, H i ). 0.21 - 0,35 (m, 2H), 0.02 - 0.18 (m, IH).

Mass Spectrum (LCMS, ESI pos.): Calcd. for (VI Ι -,,ΓΧΟ,,: 484.2 (M+H); found: 484.2,

Cpd No. Characterization

(S)-3-(3-((4-(Cyclopentyloxy)-5-(2-fluoro-5- methoxyphenyl)isoxazol-3-yl)methoxy)phenyl)-3- cyclopropylpropanoic acid

^LNMR (400 MHz, DMSO-f/ ₆ ) δ (pprn): 7.35 - 7.41 (m, 1 H), 7.29 - 7.32 (in. ! ! ! }. 7,21 - 7.25 (m, I I I ). 7.14 - 7.20 (rn. I I I ). 6.96 - 7.01 (m, 1 H), 6,88 - 6.92 (m, 2H), 5,21 (s, 2H), 4,61 (s,

40

1H), 3.82 (s, 3H), 2.53 - 2.68 (m, 2H), 2.23 - 2.36 (m, 1H), 1.67 - 1.75 (m, 2H), 1.55 - 1.67 (m, 4H), 1.41 - 1.52 (m, 2H), 0.93 - 1.05 (m, 1H), 0.45 - 0.53 (m, 1H), 0.22 - 0.31 (m, 2H), 0.06 - 0.17 (m, 1 H).

Mass Spectrum (LCMS, ESI pos. ): Calcd. for (V. _s I I .-„F\O _f ,: 496.2 (M+H); found: 496.2.

(35)-3-Cyciopropyi~3~(3~[[4-(cyclopropylmethoxy)-5-(2-fluoro - 5-methoxyphenyl)isoxazol-3-yl]methoxy]phenyl)propanoic acid Ή-NM (400 MHz, DMSO-de) δ (pprn): 7.33 - 7,41 (m, 2H), 7.12 - 7.22 (m, 2H), 6.79 - 6.92 (m, 3H), 5.21 (s, 2H), 3,81 -

41

3.83 (m, 5H), 2.27 - 2.50 (rn, 3H), 0,87 - 1.23 (m, 2H), 0.39 - 0.45 (m, 3H), 0.16 - 0.28 (m, 4H), 0.04 - 0.10 (m, 1 H). Mass Spectrum (LCMS, ESI pos.): Calcd. for

482,2(M+H); found: 482.2,

Cpd No. Characterization

(3 _^ S)-3-Cyclopropyl-3-(3-((5-(2-fluoro-5-methoxyphenyl)-4 - i sopropoxyisoxazol -3 -y l)meth oxy)pheny l)propanoic acid

' l l-NMR (300 MHz, DMSO-d ₆ ) δ (ppra): 7.23 - 7.36 (m, 2H), 7.10 - 7,20 (in, 2H), 6.88 - 6.96 (m, 3H), 5.19 (s, 2H), 4. 19 -

42 4.27 (m, IH), 3.80 (s, 3H), 2.62 - 2,65 (m, 2H), 2.26 - 2.32 (m,

H i ) 1.14 - 1 .16 (d, ./ 61 1/. 61 1 ). 0.98 - 1.01 (m, IH), 0.47 - 0.50 (m, IH), 0.22 - 0.32 (m, 2H), 0.06 - 0.1 1 ( in. III).

Mass Spectrum (LCMS, ESI pos.): Calcd. for (VI ,! ^; \0,.:

470.5 (M+H); found: 470.1 .

(3 ₁ S)-3-Cyclopropyl-3-(3-((5-(5-fluoro-2-methoxypyridin-4 -yl)- 4-isobutoxyisoxazol-3-yl)methoxy)phenyl)propanoic acid

' l l-NMR (300 MHz, DMSO-d ₆ ) δ (ppm): 8.14(s, I H), 6.98 - 7.04 (m, 2H), 6.66 - 6.72 (m, 3H), 5.03 (s, 2H), 3.80(s, 3H), 3.74

47 - 3.76 (d, ./ 6 Hz, 2H), 2.57 - 2.61 (m, 2H), 2.28 - 2.34 (m,

IH), 1.84 - 1.91 (m, IH), 0.94 - 0.99 (m, IH), 0.84 - 0.86 (d, ./ 6 Hz, 6H),0.47 - 0.51 (m, IH), 0.21 - 0.35 (m, 2H), 0.02 - 0. 1 Hi m. IH). Mass Spectrum (LCMS, ESI pos.): Calcd. for

C26H29FN2O6: 485,2 (M+H); found: 485,2.

Example 14

(35)-3-Cyclopropyl-3-(3-((5-(2-fluoro-5-methoxyphenyl)-4-((2 ,2,3,3- tetramethylcyclopropyl)methoxy)isoxazol-3 -y l)methoxy )pheny l)propanoic acid (Cpd 11)

A. Ethyl 4-(benzy]oxy)-5-(2-i1uoro-5-methoxypheny])isoxazoie-3-carbox ylate, 14a

The mixture of ethyl 4-(benzyloxy)-5-bromo-l,2-oxazole-3-carboxylate (3.0 g, 9,2 mmoi, as prepared in Example, Step B), (2-fluoro-5-methoxyphenyl)boronic acid (2.35 g, 13.8 mmol), Pd(dppf)Cl ₂ (340 mg, 0.46 mmol), Cs ₂ C0 ₃ (9 g, 27.6 mmol) in water (12 mL) and dioxane (48 mL) was stirred for 1 h at 90 °C. The reaction mixture was allowed to cool to RT and treated with satd. NH ₄ CI (aq., 60 mL). The resulting solution was extracted with EtOAc (3 x 60 mL) and the organic layers combined and concentrated under reduced pressure. The residue obtained was purified by flash chromatography (EtO Ac/petroleum ether 15:85) on silica gel to obtain compound 14a as a yellow solid. Mass Spectrum (LCMS, ESI pos.): Calcd. for C ₂ oH _i8 FN0 ₅ : 372, 1 (M+H); found: 372,0.

B. Ethyl 5~(2-fluoro~5~methoxyphenyl)~4~hydroxyisoxazole-3-carboxylat e, 14b

A mixture of compound 14b (2.0 g, 5.4 mmol), Pd on carbon (10 %, 0.5 g), methanol (30 mL) was stirred for 0.5 h at RT under 1¾ (g) (3 atm). The solids were removed by filtration. The filtrate was concentrated under reduced pressure to provide compound 14b as a white solid. Mass Spectrum (LCMS, ESI pos.): Calcd. For (>,! h ·!· \ί) 282.1 (M+H); found: 282.0.

C. Ethyl 5-(2-fluoro-5-methoxyphenyl)-4-((2,2,3,3- tetramethylcyclopropyl)methoxy)isoxazole-3-carboxylate, 14c

To a solution of compound 14b (400 mg, 1.42 mmol), (2,2,3,3- tetramethylcyclopropyl)methanol (184 mg, 1.44 mmol) and (n-Bu) ₃ P (720 mg, 3.58 mmol) in toluene (20 mL), a solution of ADDP (922 mg, 3.68 mmol) in toluene (20 mL) was added dropwise with stirring. The resulting solution was stirred overnight at 60 °C, allowed to cool to RT and then quenched by the addition of satd. NH ₄ C1 (aq. 50 mL). The resulting solution was extracted with EtO Ac (3 x 50 mL). The organic layers were combined and concentrated. The residue obtained was purified by flash chromatography (EtO Ac/petroleum ether 1 : 10) on silica gel to obtain compound 14c as colorless oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for C ₂ iH ₂₆ FN0 ₅ : 392.2 (M+H); found: 392.0.

D. (5-(2-Fluoro-5-methoxyphenyl)-4-((2,2,3,3- tetramethylcyclopropyl)methoxy)isoxazol-3-yl)methanol, 14d

To a stirring solution of compound 14c (324 mg, 0.83 mmol) in THF (10 mL) was added L1AIH ₄ (63 mg, 1.66 mmol) in portions at 0 °C. The resulting solution was stirred for 2 h at RT and quenched by the addition of satd. NH ₄ CI (aq. 10 mL). The solids were removed by filtration and the resulting solution was washed with EtO Ac (3 x 10 mL). The organic washings were combined and concentrated. The residue obtained was purified by flash chromatography (EtO Ac/petroleum ether 1 : 5 -1 :3) on silica gel to obtain compound 14d as a colorless oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for C ₁₉ H ₂ 4FN0 ₄ : 350.2 (M+FI); found: 350.0.

E. 3-(Chloromethyl)-5-(2-fluoro-5-methoxyphenyl)-4-((2,2,3,3- tetramethylcyciopropyl)methoxy)isoxazoie, 14e

14e

To a stirring solution of compound 14d (152 mg, 0.44 mmol) in DCM (10 mL) and DMF (1 mL) was added thionyl chloride (104 nig, 0.87 mmol) dropwise at 0 °C. The resulting solution was stirred for 30 mm at 0 °C. The reaction was then quenched by the addition of satd. sodium bicarbonate (aq. 50 mL). The resulting solution was extracted with EtOAc (3 x 50 mL). The organic layers were combined and dried over anhydrous sodium sulfate and concentrated to give the title compound as a yellow oil.

(3«S)-Methyl 3-cyclopropyl-3-(3-((5-(2-fluoro-5-methoxyphenyl)

-4-((2,2,3,3-tetramethylcyclopropyl)methoxy)isoxazol-3-yl)me thoxy)phenyl) propanoate, 14f

A solution of compound 14e (141 mg, 0.38 mmol), (iS')-methyl 3-cyclopropyl-3-(3- hydroxyphenyl)propanoate (101 mg, 0.46 mmol) and CS ₂ CO ₃ (248 mg, 0.76 mmol) in CH ₃ CN (10 mL) was stirred for 2 h at 50 °C. The reaction was allowed to cool to RT and treated with water (50 mL). The resulting solution was extracted with EtOAc (3 x 50 mL). The organic layers were combined and concentrated. The residue obtained was purified by flash chromatography (EtO Ac/petroleum ether) on silica gel to obtain compound 14f as a colorless oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for (\.| hsl- ^' NO,,: 552.3 (M+H); found: 552.1.

G. (3S)-3-Cyclopropyl-3-(3-((5-(2-fluoro-5-methoxyphenyl)-4-((2 ,2 ₎ 3 _J 3- tetramethylcyclopropyl)methoxy)isoxazoi-3-yl)methoxy)phenyl) propanoic acid, Cpd 11

A mixture of compound 14f (1 10 mg, 0.20 mmol) and LiOH (50 mg, 2.09 mmol) in THF (5 mL), water (2 mL) and methanol (1 mL) was stirred overnight at RT. The resulting mixture was concentrated to remove organic solvents. The pH value of the resulting solution was adjusted to 4-5 with 1 N hydrogen chloride solution. The solids formed were collected by filtration and dried in an oven under reduced pressure to obtain the title compound 11 as a light yellow solid. 1H-NMR (300 MHz, DMSO-de) δ (ppm): 7.32 - 7.40 (m, 2H), 7,22 - 7.27 (m, i l l). 7, 12 - 7.17 (m, 1 1 1). 6.89 - 6,96 (nr. 3H), 5.21 (s, 2H), 4.06 (d, J ------- 7.8 Hz, 2H), 3.80 (s, 3H), 2.62 - 2,76 (m, 2H), 2.26 - 2.33 (m, IH), 0.82 - 1.03 (m,

14H), 0.45 - 0,50 (m, 2H), 0.22 - 0,33 (m, 2H), 0.03 - 0.10 (m, IH). Mass Spectrum (LCMS, ESI pos,): Calcd. for C ₃₁ H ₃ 6FN0 ₆ : 538.3 (M+H); found: 538.2.

Following the procedures described in Example 14, and substituting suitably selected reagents, starting materials and conditions as would be readily apparent to those skilled in the art, the follo wing representative compounds of the present invention were prepared.

Cpd No. Characterization

( _i S ^' )-3-Cyclopropyl-3-(3-((5-(5-fluoro-2-methoxypyridin-4- yl)-4- ((3,3,5,5-tetramethylcyclohexyl)oxy)isoxazol-3- yl)methoxy)phenyl)propanoic acid

¹ H-NM (400 MHz, DMSO-i ₆ ) δ (pprn); 8.40 (s, IH), 7.21 - 7.27 (rn, 2H), 6.88 - 6.94 (m, 3H), 5.20 (s, 2H), 4.32 - 4.38 (m,

2 I I I). 3.89 (s, 31 1). 2.61 - 2,65 (m, 21 1 ). 2.24 - 2,30 (m, IH), 1.74 - 1.76 (d,■/ 8 Hz, 2H), 1.09 - 1.15 (m, 3H), 0.96 - 1.03 (m, 2H), 0.83 (s, 6H), 0.73 (s, 6H), 0.46 - 0.50 (m, IH), 0.22 - 0.28 (m, 2H), 0.09 - 0.11 (m, IH).

Mass Spectrum (LCMS, ESI pos. ): Calcd. for C32H39FN2O6: 567.3 (M+H); found: 567.3.

(»S -3-Cyclopropyl-3-(3-((5-(2-fluoro-5-methoxyphenyl)-4- ((3,3,5,5-tetramethylcyclohexyl)oxy)isoxazol-3- yl)methoxy)phenyl)propanoic acid

¹ H-NMR (400 MHz, DMSO-i¾): δ (pprn): 7.31 - 7,35 (m, IH), 7.25 - 7.27 (m, I H), 7.14 - 7.17 (m, 2H), 6,86 - 6.91 (m, 3H),

3 5.14 (s, 2H), 4.21 - 4,28 (m, IH), 3.80 (s, 3H), 2.34 - 2,45 (m, 3H), 1.69 - 1.71 (d, ./ 8 Hz, 2H), 1.05 - 1.14 (rn, 3H), 0.93 - 1.02 (rn, 2H), 0.81 (s, 6H), 0.69 (s, 6H), 0.42 - 0.46 (m, I H), 0.24 - 0.29 (m, 2H), 0.06 - 0.08 (m, IH).

Mass Spectrum (LCMS, ESI pos.): Calcd. for C ₃₃ H40FNO6: 566.3 (M+H); found: 566.3.

Cpd No. Characterization

( _i S ^' )-3-Cyclopropyl-3-(3-((5-(5-fluoro-2-methoxypyridin-4- yl)-4- ((3,3,4,4-tetramethylcyclopentyl)oxy)isoxazol-3- yl)methoxy)phenyl)propanoic acid

¹ H-NM (300 MHz, DMSO-i ₆ ) δ (ppm): 8,39 (s, 1H). 7.18 - 7.24 (m, 2H), 6.87 - 6.93 (m, 3H), 5.22 (s, 2H), 4.74 - 4.77 (m,

4 1 H), 3.89 (s, 3H), 2.51 - 2,53 (m, 2H), 2.32 - 2,38 (m, IH), 1 .89

- 1.97 (m, 2H), 1.68 - 1.74 (m, 2H), 0.91 - 0.94 (m, IH), 0.84 (s, 6H), 0.70 (s, 6i l h 0.43 - 0.45 (m, IH), 0.23 - 0.27 (m, 2H), 0.07

- 0.10 (m, IH).

Mass Spectrum (LCMS, ESI pos.): Calcd. for C31H37FN2O6:

553.3 (M+H); found: 553.3.

Example 15 (35)-3-Cyclopropyl-3-(3-((4-((3,3-dimethylcyclobutyl)methoxy )-5-(2-fluoro- -methoxyphenyl)isoxazol-3-yl)methoxy)phenyl)propanoic acid (Cpd 5)

o a stirring solution of ethyl 4-hydroxy-l,2-oxazole-3-carboxylate (551 nig, 3.51 nimol, s prepared in Example 13, Step B), (3,3-dimethylcyclobutyl)methanol (400 mg, 3.50 mmol) and n- u^P (2.13 g, 10.6 mmol) in toluene (10 mL) was added a solution of ADDP (1.78 g, 7.09 mmol) in toluene (10 mL) dropwise at 0 °C. The resulting solution was stirred overnight at 60 °C. The resulting mixture was allowed to cool to RT and concentrated. The residue obtained was purified by flash chromatography

(EtO Ac/petroleum ether 1 :20) on silica gel to obtain compound 15a as a colorless oil). Mass Spectrum ( i CMS. ESI pos.): Calcd. for Ci ₃ H ₁₉ N0 ₄ : 254.1 ( M i l ); found: 254.1 ,

B . (4-((3 , 3 -Dimethylcy clobuty l)methoxy) isoxazol-3 -yljmethanol, 15b

To a stirring solution of compound 15a (670 mg, 2.65 mmol) in THF (20 mL) was added L1AIH ₄ (213 mg, 5.61 mmol) in portions at 0 °C. The resulting solution was stirred for 2 h at 0 °C and then treated with H ₂ 0 (1 mL). The solids formed were removed by filtration. The filter cake was washed with DCM/EtOAc (1 : 1 , 3 x 30 mL). The organic filtrates were combined and concentrated. The residue obtained was purified by flash chromatography (EtO Ac/petroleum ether 1 :5) on silica gel to obtain compound 15b as a colorless oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for C , , l Ι , -ΝΟ ··: 212.1 (M+H); found: 212.1.

C. (5-Bromo-4-((3,3-dimethylcyclobutyl)methoxy)isoxazol-3-yl)me thanol, 15c

To a stirring solution of compound 15b (430 mg, 2.04 mmol) in DMF (1 mL) was added a solution of NBS (1.09 g, 6.12 mmol) in DMF (5 mL) dropwise. The resulting solution was stirred for 2 h at 50 °C. The reaction mixture was allowed to cool to RT, treated with H ₂ 0 (50 mL), and extracted with EtOAc (3 x 100 raL), The organic layers were combined and concentrated. The residue obtained was purified by flash chromatography

(EtO Ac/petroleum ether 1 :5) on silica gel to obtain compound 15c as a colorless oil). Mass Spectrum (LCMS, ESI pos,): Calcd, for CnH ₁₆ BrN0 ₃ : 290.0 (M+H); found: 290.0.

D. 5-Bromo-3-(chloromethyl)- -((3,3-dimethylcyclobutyl)methoxy)isoxazole, 15d

To a stirring solution of compound 15c (310 mg, 1.07 mmol) in DMF (1 mL) and DCM (10 mL) was added a solution of thionyl chloride (383 mg, 3.22 mmol) in DCM (2 mL) dropwise at 0 °C. The resulting solution was stirred for 2 h at 0 °C and then quenched with satd. NaHC0 ₃ (aq. 5 mL). The resulting solution was extracted with EtO Ac (3 x 50 mL). The organic layers were combined and dried over Na ₂ S0 and concentrated to give compound 15d as a colorless oil).

E. (,S)-Methyl 3-(3-((5-bromo-4-((3,3-dimethylcyclobutyl)methoxy)

isoxazol-3-yl)methoxy)phenyl)-3-cyclopropylpropanoate, 15e

A mixture of compound 15d (287 mg, 0.93 mmol), (iS')-methyl 3-cyclopropyl-3-(3- hydroxyphenyl)propanoate (307 mg, 1.39 mmol) and Cs ₂ C0 ₃ (757 mg, 2.32 mmol) in CH ₃ CN (10 mL) was stirred overnight at RT. The reaction was then quenched with water (5 mL). The resulting solution was extracted with EtOAc (3 x 20 mJL). The organic layers were combined and concentrated. The residue obtained was purified by flash

chromatography (EtO Ac/petroleum ether 1 : 10) on silica gel to obtain compound 15e as a colorless oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for (\.. ₄ I lir\O<: 492.1 (M+H); found: 492.1.

F. (35)-Methyl 3-cyclopropyl-3-(3-((4-((3,3-dimethylcyclobutyl)methoxy)-5- (2-fluoro-5-methoxyphenyl)isoxazol-3-yl)methoxy)phenyl)propa noate, 15f

A mixture of compound 15e (100 mg, 0.20 mmol), (2-fluoro-5-methoxyphenyl)boronic acid (75 mg, 0.44 mmol), Pd(dppf)Cl ₂ (8 mg, 0.01 mmol) and potassium carbonate (75 mg, 0.54 mmol) in DME (0.8 mL) and water (0.2 mL) was stirred overnight at 80 °C under N _∑ . The resulting mixture was concentrated and the residue obtained was purified by flash chromatography (10-20% EtO Ac/petroleum ether) on silica gel to obtain compound 15f as a colorless oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for ί\< ! ! .ΙΛΟ-,: 538.3 (M+H); found: 538.3.

G. (3S)-3-cyclopropyl-3-(3-((4-((3,3-dimethylcyclobutyl)methoxy )-5-(2-fluoro-5- methoxyphenyl)isoxazol-3-yl)methoxy)phenyl)propanoic acid, Cpd 5

A mixture of compound 15f (52 mg, 0.10 mmol) and LiOH (25 mg, 1.04 mmol) in THF (2 mL), water (1 mL), and methanol (0.5 mL) was stirred overnight at RT. The resulting mixture was concentrated to remove organic solvents and the pH of the resulting solution was adjusted to 3 - 4 with 1 N HC1 solution. The solids formed were collected by filtration and dried in an oven under reduced pressure to obtain the title compound 5 as a yellow solid. 1H-NMR (300 MHz, DMSO-d ₆ ) δ (ppm): 7.28 - 7.37 (m, 1H), 7,23 - 7.28 (m, 2H), 7.13 - 7.18 (in, 1H), 6.89 - 6.96 (m, 3H), 5.21 (s, 2H), 3.92 (d, J = 6.6 Hz, 2H), 3.80 (s, 3H), 2.64 - 2,68 (m, 2H), 2.47 - 2.50 (m, IH), 2.26 - 2.29 (m, IH), 1.67 - 1.70 (m, 2H), 1.47 ·· 1.64 (m, 2H), 0.92 ·· 1.05 (m, 7H), 0.40 - 0.56 (m, IH), 0.20 - 0.40 (m, 2H), 0.02 - 0.11 (m, IH). Mass Spectrum (LCMS, ESI pos.): Calcd. for CJ i-, ! ^; NO,..: 524.2 (M+H); found: 524.3.

Following the procedures described in Example 15, and substituting suitably selected reagents, starting materials and conditions as would be readily apparent to those skilled in the art, the following representative compound of the present invention was prepared.

Example 16

-cyclopropyl-3-(3-((5-(5-fluoro-2-methoxypyridin-4-yl)-4-(2- methylprop-l enyl)isoxazo3-3-yl)methoxy)pheny])propanoic acid (Cpd 52)

A. Ethyl 4-(benzyloxy)isoxazol -3-carboxylate, 16a

A mixture of ethyl 4-hydroxyisoxazole-3-carboxylate (3.0 g, 19 mmol, as prepared in Example 13, Step B), (bromomethyl)benzene (3.6 g, 21 mmol) and Cs ₂ C0 ₃ (9.4 g, 29 mmol) in DMF (50 mL) was stirred for 2 h at 50 °C. The reaction mixture was allowed to cool to RT and treated with H ₂ 0 (50 mL). The resulting solution was extracted with EtO Ac (3 x 50 mL). The organic layers were combined, dried over Na ₂ S0 ₄ and concentrated. The residue obtained was purified by flash chromatography (0-15%

EtO Ac/petroleum ether) on silica gel to obtain compound 16a as a white solid. Mass Spectrum ( I .C.MS. ESI pos.): Calcd. for C13HJ 3NO4: 248.1 (M+H); found: 248.0,

B. Ethyl 4-(benzyloxy)-5-bromoisoxazole-3-carboxylate, 16b

A solution of compound 16a (3.7 g, 15 mmol) and NBS (4 g, 22.5 mmol) in DMF (50 mL) was stirred overnight at 60 °C. The reaction mixture was allowed to cool to RT and treated with satd. NH ₄ CI (aq. 100 mL). The resulting solution was extracted with EtO Ac (3 x 100 mL). The organic layers were combined, dried over Na ₂ S0 ₄ and concentrated. The residue obtained was purified by flash chromatography (0-10% EtO Ac/petroleum ether) on silica gel to obtain compound 16b as a yellow oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for Ci ₃ Hj ₂ BrN0 ₄ : 326.0 (M+H); found: 325.9.

C. Ethyl 4-(benzyloxy)-5-(5-fluoro-2-methoxypyridin-4-yl)isoxazole-3- carboxylate,

16c

A mixture of compound 16b (550 mg, 1.69 mmol), (5-fluoro-2-methoxypyridin-4- yl)boronic acid (433 mg, 2.53 mmol), Cs ₂ C0 ₃ (1.65 g, 5.06 mmol), Pd(dppf)Cl ₂ (123 mg, 0.17 mmol) in water (3 mL) and 1,4-dioxane (12 mL) was stirred for 1 h at 80 °C under N ₂ . The reaction mixture was allowed to cool to RT and treated with said. NH ₄ CI (aq. 1 5 mL). The resulting solution was extracted with EtOAc (3 x 15 mL). The organic layers were combined, dried over Na ₂ S0 and concentrated under reduced pressure. The residue obtained was purified by flash chromatography (0-15% EtO Ac/petroleum ether) on silica gel to obtain compound 1.6c as a colorless oil. Mass Spectrum (LCMS, ESI pos.); Calcd. for CYJ f rFN ··(}÷: 373.1 (M+H); found: 373.1 ,

D. Ethyl 5-(5-fluoro-2-methoxypyridin-4-yl)-4-hydroxyisoxazole-3-carb oxylate, 16d

A mixture of compound 18c (450 mg, 1.21 mmol) and Pd/C (10 %, wet, 1 50 mg) in methanol (15 mL) was stirred for 30 min at RT under a H ₂ (g) (3 atm). The solids were removed by filtration and the filtrate was concentrated to give compound 16d as a yellow solid. Mass Spectrum (LCMS, ESI pos.): Calcd. for Cj HnF Os: 283.1 (M+F); found: 283.0.

E. Ethyl 5-(5-fluoro-2-methoxypyridin-4-yl)-4-(trifluoromethylsulfony l-oxy)isoxa- zole-3-earboxylate, 16e

To a stirring solution of compound 16c! (320 mg, 1.13 mmol) and 2,6-dimethylpyridine (182 mg, 1.70 mmol) in DCM (10 mL) was added Tf ₂ 0 (182 mg, 0.65 mmol) dropwise. The resulting solution was stirred for 1 h at RT. The reaction mixture was treated with said. NH ₄ CI (aq. 10 mL) and extracted with EtOAc (3 x 10 mL). The organic layers were combined, dried over Na ₂ S0 ₄ and concentrated. The residue obtained was purified by flash chromatography (0-10% EtO Ac/petroleum ether) on silica gel to obtain compound 16e as a colorless oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for C13H10F4N2O7S: 415.0 (M+H); found: 414.9.

F. Ethyl 5-(5-fluoro-2-methoxypyridin-4-yl)-4-(2-methylprop-l -enyl)isoxazole-3- carboxylate, 16f

A mixture of compound 16e (450 mg, 1.09 mmol), 4,4,5, 5-tetramethyl-2-(2-methylprop-l - en-l-yl)-l,3,2-dioxaborolane (300 mg, 1.65 mmol), Pd(dppf)Cl ₂ (80 mg, 0.1 1 mmol) and CS2CO3 (880 mg, 2.70 mmol) in water (2 mL) and 1 ,4-dioxane (8 mL) was stirred for 0.5 h at 85 °C under N ₂ . The reaction mixture was allowed to cool to RT and treated with ¾0 (10 rnL). The resulting solution was extracted with EtOAc (3 x 10 raL), The separated organic layers were combined, dried over Na ₂ S0 ₄ and concentrated. The residue obtained was purified by flash chromatography (0-10% EtOAc/petroleum ether) on silica gel to obtain compound 16f as a colorless oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for Ci ₆ H ₁₇ FN ₂ 0 ₄ : 321.1 (M+H); found: 321.0.

G. (3 V3-cyclopropyl-3-(3-((5-(5-fluoro-2-methoxypyridin-4-yl)-4-( 2-methylprop-l- enyl)isoxazol-3-yl)methoxy)phenyl)propanoic acid, Cpd 52

Compound 52 was prepared from compound 16f following the procedures described in

Example 3, Steps E-H. ^l H-NMR (400 MHz, DMSO-d ₆ ) δ (ppm): 8.38 (d, ./ 2.0 Hz, I I I). 7.18 (t, ./ 8.0 Hz, 1H), 7.09 (d, J= 4.8 Hz, 1H), 6.81 - 6.88 (m, 3H), 6.02 (s, IH), 5.14 (s, 2H), 3.88 (s, 3H), 2.29 - 2.48 (m, 3H), 1.87 (s, 3H), 1.31 (s, 3H), 0.88 - 0.95 f ro. IH), 0.42 - 0.45 (m, IH), 0.22 - 0.34 (m, 2H), 0.04 - 0.07 (m, IH). Mass Spectrum (LCMS, ESI pos.): Calcd. for ( j ! -Ι- ·.(),: 467.2 (M+H); found: 467.2.

Example 17

(35)-3-Cyclopropyl-3-(3-((5-(5-fluoro-2-methoxypyridin-4-yl) -4-isobutylisoxazol-3- yi)meth-oxy)phenyl)propanoic acid (Cpd 12)

A mixture of compound 52 (100 mg, 0.21 mmol, as prepared in Example 16) and 10% palladium on carbon (50 mg) in methanol (10 raL) was stirred 3 h at RT under a H ₂ (g) (3 atm). The solids were removed by filtration. The filtrate was concentrated and the residue obtained was purified by flash chromatography (0-95% DCM/methanol) on silica gel to obtain compound 12 as a gray solid. 1H-NMR (400 MHz, DMSO-d ₆ ) δ (ppm): 8.41 (s, 1 1 1 ). 7.21 (t, J 7.6 Hz, ! ! ! }. 7.15 (d. ./ 4.4 Hz, 1H), 6.86 - 6.92 (m, 3H), 5.22 (s, 2H), 3.90 (s, 3H), 2.43 - 2.51 (m, 4H), 2,31 - 2.33 (m, IH), 1.75 - 1.78 (m, IH), 0.91 - 0.95 (m, IH), 0.74 (d, J = 6.8 Hz, 6H), 0.43 - 0.45 (m, IH), 0.25 - 0.28 (m, 2H), 0.06 - 0.07 (m, IH). Mass Spectrum (LCMS, ESI pos.): Calcd. for C26H29FN2O5: 469.2 (M+H); found: 469.3.

Example 18

(35)-3-Cyclopropyl-3-(3-((4-ethoxy-5-(2-fluoro-5-methoxyphen yl)isoxazol-3- yl)methoxy)phenyl)propanoic acid (Cpd 50)

A. Ethyl 4-(neopentyloxy)isoxazole-3-carboxylate, 18a

To a stirring solution of ethyl 4-hydroxyisoxazole-3-carboxylate (1.5 g, 9.6 mmol, as prepared in Example 13, Step B), 2,2-dimethylpropan-l -ol (1.26 g, 14.3 mmol) and «-Bu ₃ P (40.4 g, 20 mmol) in toluene (20 mL) was added a solution of ADDP (5.1 g, 20.2 mmol) in toluene (20 mL) dropwise at 0 °C. The resulting solution was stirred overnight at 60 °C.

The reaction mixture was allowed to cool to RT and treated with satd. NH ₄ CI (aq. 50 mL).

The resulting solution was extracted with EtOAc (3 x 20 mL). The organic layers were combined, dried over Na ₂ S0 ₄ and concentrated. The residue obtained was purified by flash chromatography (0-5% EtOAc/ petroleum ether) on silica gel to obtain compound

18a as a light yellow solid. Mass Spectrum (LCMS, ESI pos. ): Calcd. for : CnH ₁₇ N0 ₄ :

228.1 (M+H); found: 228.1. B Ethyl 5-bromo-4-(neopentyloxy)isoxazole-3-carboxylate, 18b

18b

A solution of compound 18a (500 mg, 2.64 mmol) and NBS (941 mg, 5.29 mmol), in DMF (6 mL) was stirred overnight at 80 °C. The reaction mixture was allowed to cool to RT and was then quenched with satd. NH ₄ CI (aq. 20 mL). The resulting solution was extracted with EtOAc (3 x 10 mL), and the organic layers were combined, dried over Na ₂ SQ ₄ and concentrated. The residue obtained was purified by flash chromatography (0- 5 % EtO Ac/petroleum ether) on silica gel to obtain compound 18b as light yellow oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for C, , 1 l _i .,Br\0 ₄ : 306.0 (M+H); found: 305,9.

C. Ethyl 5~(2-fluoro~5~methoxyphenyl)~4~(neopentyloxy)isoxazole-3-car boxylate, 18c

A mixture of compound 18b ( 50 mg, 0.49 mmol), (2-fluoro-5-methoxyphenyl)boronic acid (167 mg, 0.98 mmol), Pd(dppf)Cl ₂ (20 mg, 0.02 mmol) and Cs ₂ CQ ₃ (320 mg, 0.98 mmol) in water (0.5 mL) and 1 ,4-dioxane (2 ml.) was stirred for 1 h at 80 °C. The reaction mixture was allowed to cool to RT and quenched with satd. NH ₄ C1 (aq. 5 mL). The resulting solution was extracted with EtOAc (3 x 10 mL). The organic layers were combined, dried over Na ₂ S0 ₄ and concentrated. The residue obtained was purified by flash chromatography (0-5% EtOAc/ petroleum ether) on silica gel to obtain compound 18c as a light yellow oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for C _i8 H ₂₂ FN0 ₅ : 352.1 (M+H); found: 352,0. D. (4-Ethoxy-5-(2-fluoro-5-methoxyphenyl)isoxazol-3-yl)methanol , 18d

To a stirring solution of compound 18c (120 mg, 0.34 mmol) in THF (3 ml.) at 0 °C was added LiAiH ₄ (26 mg, 0.69 mmol) portionwise. The resulting solution was stirred at 0 °C for 30 min. and quenched by the addition of 2 g of Na ₂ SO ₄ 10H ₂ O. The solids were removed by filtration and the filtrate was concentrated. The residue obtained was purified by flash chromatography (0-20% EtOAc/ petroleum ether) on silica gel to obtain compound 18d as a light yellow oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for Ci ₃ Hi ₄ FN0 ₄ : 268.1 (M 1 1); found: 268.0.

D. (3S)-3-cyclopropyl-3-(3-((4-ethoxy-5-(2-fluoro-5-methoxyphen yl)isoxazol-3- yi)methoxy)phenyl)propanoic acid, Cpd SO

Compound 50 was prepared from compound 18d following the procedures described in Example 3, Steps F-H. Ή-NM (300 MHz, DMSO-d6) δ (ppm): 11.95 (br, IH), 7.13 - 7.40 (m, 4H), 6.89 - 6.97 (m, 3H), 5.22 (s, 2H), 4.03 (q, J, = 7.2 Hz, J ₂ = 14.1 Hz, 2H), 3.81 (s, 3H), 2.64 - 2.68 (m, 2H), 2.25 ·· 2.28 (m, IH), 1.21 (t, J = 7.2 Hz, 3H), 0.93 ·· 1.02 (m, IH), 0.41 - 0.49 (m, IH), 0.24 - 0.29 (m, 2H), 0.07 - 0, 12 (m, IH). Mass Spectrum (LCMS, ESI pos,): Calcd. for CasHaeFNOe: 456.2 (M+H); found: 456.1.

Following the procedures described in Example 18, and substituting suitably selected reagents, starting materials and conditions as would be readily apparent to those skilled in the art, the following representative compound of the present invention was prepared. Cpd No. Characterization

(5)-3-Cyclopropyl-3-(3-((4-ethoxy-5-(5-fluoro-2- methoxypyridin-4-yl)isoxazol-3-yl)methoxy)phenyl)propanoic acid

lH-NMR (300 MHz, OM$Q-d ₆ ) δ (ppm): 1 1 .94 - 12.1 1 (br.m, 1H), 8.40 (d, ,/ 2.1 Hz, 1 H), 7,21 - 7.26 (m, 2H), 6, 89 - 6.97

51 (m, 3H), 5,27 (s, 2H), 4.12 (q, J= 6.9 Hz, 2H), 3.90 (s, 3H), 2.56

- 2,65 (m, 2H), 2.26 - 2,31 (m, i l l ). 1.25 (t, J = 6.3 Hz, 3H), 0.98

- 1.02 (m, 1H), 0.48 - 0.52 (m, 1H), 0,28 - 0.31 (m, 2H), 0,06 - 0.11 (m, H i ).

Mass Spectrum (LCMS, ESI pos.): Calcd. for C24H25FN2O6:

457.2 ( M i l ); found: 457.1 ,

Example 19

(35 -3-Cyclopropyl-3-(3-((5-(2-fluoro-5-methoxyphenyl)-4-(3,3,3- trifluoropropoxy)isoxazol-3-yl)methoxy)phenyl)propanoic acid (Cpd 49)

A. Ethyl 4-(3,3,3-trifluoropropoxy)isoxazole-3-carboxylate, 19a

To a stirring solution of ethyl 4-hydroxyisoxazole-3-carboxylate (600 mg, 3.82 mmol, as prepared in Example 13, step B), 3,3,3-trifluoropropan-l-ol (653 mg, 5.72 mmol) and n- BU3P (1.54 g, 7.62 mmol) in toluene (60 mL) was added ADDP (1.93 g, 7.71 mmoi) portionwise at 0 °C. The resulting solution was stirred overnight at 60 °C. The reaction mixture was allowed to cool to RT and treated with said. NH ₄ CI (aq. 100 mL). The resulting solution was extracted with EtOAc (3 x 100 mL). The organic layers were combined, dried over Na ₂ S0 ₄ and concentrated. The residue obtained was purified by flash chromatography (0-30% EtO Ac/petroleum ether) on silica gel to obtain compound 19a as a white solid. Mass Spectrum (LCMS, ESI pos.): Calcd. for C ₉ H ₀ F ₃ NO ₄ : 254.1 (M-i-H); found: 254.0. B. Ethyl 5-bromo-4-(3,3,3-trifluoropropoxy)isoxazole-3-carboxylate, 19b

A solution of compound 19a (600 mg, 2.37 mmol) and NBS (2.11 g, 1 1.9 mmol) in DMF (10 mL) was stirred overnight at 60 °C. The reaction mixture was allowed to cool to RT and treated with FLO (10 mL). The resulting solution was extracted with EtO Ac (3 x 10 mL). The organic layers were combined, dried over Na ₂ S0 ₄ and concentrated. The residue obtained was purified by flash chromatography (0-15% EtO Ac/petroleum ether) on silica gel to obtain compound 19b as a yellow solid. Mass Spectrum (LCMS, ESI pos.): Calcd. for C,i l,,Br! ^; :N0 ₄ : 332.0 (M+H); found: 332.0.

Ethyl 5-(2-fluoro-5-methoxyphenyl)-4-(3,3,3-trifluoropropoxy)

isoxazoie-3-carboxyiat

A mixture of compound 19b (450 mg, 1.36 mmol), (2-fluoro-5-methoxyphenyl)boronic acid (350 mg, 2.06 mmol), Pd(dppf)C12 (50 mg, 0.07 mmol) and Cs ₂ C0 ₃ (1.1 g, 3.4 mmol) in 1,4-dioxane (8 mL) and water (2 mL) were stirred for 1 h at 85 °C under N _∑ . The reaction mixture was allowed to cool to RT and treated with satd. NH4CI (aq. 15 mL). The resulting solution was extracted with EtOAc (3 x 15 mL). The organic layers were combined, dried over Na ₂ S0 ₄ and concentrated. The residue obtained was purified by flash chromatography (0-10% EtO Ac/petroleum ether) on silica gel to obtain compound 19c as a yellow oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for C16H15F4NO5: 378.1 (M+H); found: 378.1.

D. (3S)-3-cyclopropyl-3-(3-((5-(2-fluoro-5-methox>'phenyl)-4 -(3,3,3- trifluoropropoxy)isoxazol-3-yl)methoxy)phenyl)propanoic acid, Cpd 49

Compound 49 was prepared from compound 19c following the procedures described in Example 3, Steps E-H. 1H-NMR (300 MHz, DMSO-d ₆ ) δ (ppm): 7.27 ·· 7.37 (m, IH), 7.21 - 7.26 (m, IH), 7.12 - 7.19 (m, 2H), 6.85 - 6.93 (m, 3H), 5.22 (s, 2H), 4.20 (t, J = 6.0 Hz, 2H), 3.80 (s, 3H), 2.66 - 2.73 (m, 2H), 2.32 - 2.45 (m, 3H), 0.92 - 0.94 (m, IH), 0.41 - 0.43 (m, IH), 0.22 - 0.26 (m, 2H), 0.05 - 0.06 (m, IH). Mass Spectrum (LCMS, ESI pos.): Calcd. for .I ^; ₄ NO,..: 524.2 (M+H); found: 524.2.

Example 20

(35)-3-(3-((4-(Cyclohexyk>xy)~5~(2-fluoro~5~methoxyphenyl )isoxazol-3- yl)methoxy)phenyl)-3-cyclopropylpropanoic acid (Cpd 46)

r Ethyl 4-(cycl ohexyloxy)isoxazol e-3 -carboxy late, 20a

To a solution of ethyl 4-hydroxyisoxazole-3-carboxylate (2.0 g, 12.7 mmol, as prepared in Example 13, Step B), eyclohexanol (2.55 mg, 25.4 mmol), PPh ₃ (5.0 g, 19 mmol) in THE (20 mL) was added a solution of di-tert-butyl azodicarboxylate (3.51 g, 5.3 mmol) in THE (5 mL) dropwise with stirring. The resulting solution was stirred for 3 h at RT. The reaction was then quenched with water (30 mL). The resulting solution was extracted with EtOAc (3 x 20 mL). The organic layers were combined, dried over anhydrous Na ₂ S0 ₄ and concentrated. The residue obtained was purified by flash chromatography

(EtO Ac/petroleum ether 1 : 100-1 :30) on silica gel to obtain compound 20a as a colorless oil. Mass Spectrum (LCMS, ESI pos,): Calcd, for { , .H -NOc 240.1 (M+H); found: 240.1 ,

B. Ethyl 5-bromo-4-(cyclohexyloxy)isoxazole-3-carboxylate, 20b

A solution of compound 20a (1.1 g, 4.6 mmol) and NBS (2.05 g, 11.5 mmol) in DMF (10 mL) was stirred for 3 h at 50 °C. The reaction mixture was allowed to cool to RT and quenched with water (20 mL). The resulting solution was extracted with EtO Ac (3 x 10 mL). The organic layers were combined, dried over anhydrous Na ₂ S0 ₄ and concentrated. The residue obtained was purified by flash chromatography (EtO Ac/petroleum ether 1 : 100-1 :30) on silica gel to obtain compound 20b as a light yellow oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for C ₁₂ Hi6BrN0 ₄ : 318.0 (M+H); found: 318.0.

C. (3S)-3-(3-((4-(cyclohexyloxy)-5-(2-fluoro-5-methoxyphenyl)is oxazol-3- yl)methoxy)phenyl)-3-cyclopropylpropanoic acid, Cpd 46 Compound 46 was prepared from compound 20b following the procedures described in Example 13, Steps E-H. 1H-NMR (300 MHz, DMSO-d ₆ ) δ (ppm): 7.30 - 7.39 (m, 2H), 7.10 - 7,23 (m, 2H), 6.86 - 6.93 (m, 3H), 5.18 (s, 2H), 3.97 - 4.00 (m, III), 3.80 (s, 3H), 2.32 - 2,45 (m, 3H), 1.73 - 1 .85 (m, 2H), 1.51 - 1.62 (m, 2H), 1.28 - 1.35 (m, 3H), 1.07 - 1.18 (m, 3H), 0.98 - 1.01 (m, 1 H), 0.47 - 0.51 (m, I H), 0,21 - 0.35 (m, 2H), 0,02 - 0.18 (m, 1 1 1 ). Mass Spectrum (LCMS, ESI pos.): Caicd. for ( l i . -.i- NO ^, ,: 510.2 (M+H); found: 510.1.

Example 21

(35)-3-(3-((4-(Cyclohexyloxy)-5-(5-fluoro-2-methoxypyridin-4 -yl)isoxazol-3- yl)methoxy)phenyl)-3-cyclopropylpropanoic acid (Cpd 48)

A. (5-Bromo-4-(cyclohexyl xy)isoxazol-3-yl)methanol, 21a

To a stirring solution of compound 20b (500 mg, 1.57 mmol) in THF (10 mL) was added a solution of 1M DIBAL in THF (4.72 mL, 4.72 mmol) dropwise at -78 °C. The resulting solution was stirred for 3 h at RT. The reaction was then quenched with seignette salt solution (aq. 15 mL). The resulting solution was stirred vigorously for 1 h, and extracted with EtOAc (3 x 10 mL). The organic layers were combined, dried over anhydrous Na ₂ S0 ₄ and concentrated. The residue obtained was purified by flash chromatography (EtOAc/ petroleum ether 1 : 100-1 : 10) on silica gel to obtain compound 21a as a yellow oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for Ci ₀ H ₁₄ BrNO ₃ : 276.0 (M H i. found: 276.1.

B. (3S)-3-(3-((4-(cyclohexyloxy)-5-(5-fluoro-2-methoxypyridin-4 -yl)isoxazol-3- yl)methoxy)phenyl)-3-cyclopropylpropanoic acid, Cpd 48

Compound 48 was prepared from compound 21a following the procedures described in Example 15, Steps D-G. 1H-NMR (300 MHz, DMSO-de) δ (ppm): 8.39 (s, 1H), 7.20 - 7.27 (m, 2H), 6.87 - 6.95 (m, 3H), 5.23 (s, 2H), 4.06 - 4.13 (m, 1H), 3.93 (s, 3H), 2.55 - 2.59 (m, 2H), 2.27 - 2.34 (m, 1H), 1.73 - 1.85 (m, 211), 1.51 - 1.62 (m, 2H), 1.28 - 1.35 (m, 3H), 1.07 - 1.18 (m, 3H), 0.98 - 1.01 (m, 1H), 0.47 - 0.51 (m, 1H), 0.21 - 0.35 (m, 2H), 0.02 - 0.18 (m, III). Mass Spectrum (LCMS, ESI pos.): Calcd. for Ο _κ Ι I ; , I ^; N .(),.: 51 1.2 (M+H); found: 511.2.

Example 22 (3^)-3-(3-((5-(Cyclopentyloxy)-l-(2-fluoro-5-methoxyphenyl)- lH-l,2,3- triazol-4-yl)methoxy)phenyl)-3-cyclopropylpropanoic acid (Cpd 67)

A. (5-Chloro-l-(2-fluoro-5-m hoxyphenyl)-lH-l,2,3-triazol-4-yl)methanol, 22a

To a mixture of (5-amino-l-(2-fluoro-5-methoxyphenyl)-lH^

(1.2 g, 5,0 mmol, as prepared in Example 5, Step C), CuCl (1,04 g, 15.1 mmol) and CuCl ₂ (2.0 g, 15 mmol) in CEUCN (15 ml,) was added isoamyl nitrite (3,09 g, 26.4 mmol) at 0 ^C 'C. The resulting solution was stirred overnight at RT. The reaction was then quenched with water (20 mL), and extracted with EtOAc (3 x 20 mL). The organic layers were combined, dried over Na ₂ S0 ₄ and concentrated. The residue obtained was purified by flash chromatography (0-35% EtO Ac/petroleum ether) on silica gel to obtain compound 22a as a light yellow solid. Mass Spectrum (LCMS, ESI pos. ): Calcd. for C10H ₉ CIFN3O2: 258.0 (M+H); found: 258.0.

(36 Methyi 3-(3-((5-chloro-l-(2-fluoro-5-methoxyphenyl)-lH-l,2,3-triazo l-4- yl)methoxy)phenyl)-3-cyclopropylpropanoate, 22b

Compound 22bwas prepared from compound 2:2a following the procedures described in Example 3, Steps F-G. Mass Spectrum (LCMS, ESI pos.): Calcd. for C23H23CIFN3O4: 460.1 (M+H); found: 460, 1 .

C. (35)-3-(3-((5-(Cyclopentyloxy)-l -(2-fluoro-5-methoxyphenyl)-l H-l.,2,3-triazol-4- yl)methoxy)phenyl)-3-cyclopropylpropanoic acid, Cpd 67

To a solution of eyelopentanol (284 rag, 3.30 mmoi) in THF (3 mL) was added NaH (60% in mineral oil, 132 mg, 3.30 mmol). The resulting mixture was stirred for 1 h. To this was added a solution of compound 22b (150 mg, 0.33 mmol) in THF (1 mL). The resulting solution was stirred overnight at 75 °C. The reaction mixture was allowed to cool to RT, and water (2 mL) was added. The pH of the solution was adjusted to 4~5 with 1M HC1 solution. The resulting solution was extracted with DCM (3 x 20 mL). The organic layers were combined, dried over Na ₂ S0 ₄ and concentrated. The residue obtained was purified by Prep-HPLC using water and CLI ₃ CN as the mobile phase to the title compound was obtained as off-white solid. 1H-NMR (400 MHz, OMSO-dg) δ (ppm): 7.47 (t, J ------- 9,2 Hz,

1H), 7.20 - 7,26 (m, 3H), 6.87 - 6,96 (m, 3H), 5.18 (s, 2H), 4.97 - 4.99 (m, 1H), 3.80 (s, 3H), 2.65 - 2.68 (m, 2H), 2,25 - 2.27 (m, 1 H), 1.69 - 1.72 (m, 4H), 1.44 - 1.50 (m, 4H), 0.98 - 1 .01 (m, 1 H), 0,49 - 0.52 (m, 1 H), 0,24 - 0.32 (m, 2H), 0.13 - 0.14 (m, 1H). Mass Spectrum (LCMS, ESI pos.): Calcd. for C27H30F 3O5: 496.2 (M+H); found: 496.2,

Example 23 (3S)-3-Cyclopropyl-3-(3-((l -(2-fluoro-5-methoxyphenyl)-5-isopropox>'-lH-l,2,3-triazo l- 4-yl)methox henyl)pro panoic acid (Cpd 98)

A mixture of Na (322 nig, 14 mmol) in propan-2-ol (3 mL) was stirred for 2 h at 75 °C and was treated with compound 22b (130 mg, 0.28 mmol) in THF (1 mL). The resulting solution was stirred overnight at 75 °C. The reaction mixture was allowed to cool to T and water was added. The pH of the solution was adjusted to 6 with I HCl. The resulting solution was extracted with EtO Ac (3 x 5 mL). The combined EtOAc layers were concentrated and the residue obtained was purified by flash chromatography (0-30% EtOAc/ petroleum ether) on silica gel to obtain compound 98 as a white solid. Ή-NMR (300 Mi l/. DMSO-d ₆ ) δ (ppm): 7.48 (t, J = 9.3 Hz, H I ). 7.21 - 7,26 (m, 3H), 6.86 - 6.94

(m, 3H), 5.15 (s, 2H), 4.58 - 4.62 (m, 1H), 3.81 (s, 3H), 2.51 - 2,57 (m, 2Ή), 2,28 - 2.32 (m, 1 H), 1.14 - 1.20 (m, 6H), 0.92 - 0.98 (m, 1H), 0.42 - 0,48 (m, 1H), 0.23 - 0,28 (m, 2H), 0.06 - 0, 1 1 (m, 1H). Mass Spectrum (LCMS, ESI pos.): Calcd. for l ^l- N ;() _. *: 470.2 (M-i-H); found: 470.2.

Example 24

(35)-3-(3-((5-(Cyclohexyloxy)-l-(2-fluoro-5-methoxyphenyl)-l H-l,2,3-triazol-4- yl)methoxy)phenyl)-3-cyclopropylpropanoic acid (Cpd 97)

To a solution of cyclohexanol (326 mg, 3.25 mmol) in THF (2 niL) was added NaH (60% in mineral oil, 132 mg, 3.30 mmol). The resulting solution was stirred for 30 min at RT. To t is was added a solution of compound 22b (150 mg, 0.33 mmol) in THF (1 mL). The resulting solution was stirred for 2 days at 75 °C. After cooling, water was added. The pH of the solution was adjusted to 6 with IN HCl solution. The resulting solution was extracted with EtOAc (3 x 10 mL). The combined EtO Ac layers were concentrated and the residue obtained was purified by flash chromatography (0-30% EtOAc/ petroleum ether) on silica gel to obtain compound 97 as a brown solid. ^f H-NMR (300 MHz, DMSO- d ₆ ) δ (ppm): 1 1.99 (br, IH), 7.45 (t, 9.3 Hz, I H), 7.21 - 7.26 (m, 3H), 6.86 - 6.96 (m,

3H), 5.16 (s, 2H), 4.36 - 4.42 (m, IH), 3.80 (s, 3H), 2,63 - 2.67 (m, 2H), 2.28 - 2.32 (m, I H), 1.71 - 1.81 (m, 2H), 2.45 - 2.51 (m, 2H), 1.34 - 1.35 (m, 3H), 1.13 - 1.16 (m, 3H), 0.92 - 0.98 (in, I H), 0.42 - 0.48 (m, I H), 0.23 - 0.28 (m, 2H), 0.06 - 0.1 1 (m, IH). Mass Spectrum (LCMS, ESI pos.): Calcd. for C . _K ! !■· _. ·!· % :(),: 510.2 (M+H); found: 510.2.

Example 25

(3«S)-3-Cyclopropyl-3-(3-((l -(2-fluoro-5-methoxyphenyl)-5-(piperidin- 1 -yl)-lH- 1 ,2,3- triazol-4-yl)methoxy)phenyl)propanoic (Cpd 44)

Ethyl 5-chloro-l -(2-fluoro-5-methoxyphenyl)-l Η-1 ,2,3-triazole-4-carboxylate, 25a

Ethyl 5-amino-l-(2-fluoro-5-methoxyphenyl)-lH-l ,2,3-triazole-4-carboxylate (500 mg, 1.78 mmol, as prepared in Example 5, Step B) was added portionwise to a 20 % HQ solution (20 mL). The resulting mixture was cooled to 0 °C and treated with a solution of NaN0 ₂ (365 mg, 5.29 mmol) in H ₂ 0 (2 mL) dropwise with stirring at 0 °C. The resulting mixture was stirred for 30 min to obtain the corresponding diazonium salt solution.

A mixture of CuCl (710 mg, 7.24 mmol) in 20% HQ solution (20 mL) was stirred at 75 °C for 20 min. To the resulting solution, the diazonium salt solution prepared above was added dropwise at 75 °C. After the addition, the mixture was stirred for 2 h at 75 °C. The resulting solution was allowed to cool to RT and extracted with EtO Ac (2 x 20 mL). The organic layers were combined, washed with satd. sodium carbonate (aq. 20 mL) and concentrated. The residue obtained was purified by flash chromatography (0-30%

EtO Ac/petroleum ether) on silica gel to obtain compound 25a as a colorless oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for C _P I Ι , ,<.ΊΙ ^: \ ;0 ;: 300.0 (M+H); found: 300.0. B. Ethyl l-(2-fluoro-5-methoxyphenyl)-5-(piperidin-l-yl)-lH-l ,2,3-triazole-4- earboxyiate, 25b

A mixture of Bi NAP (92 mg, 0.15 mmol), Pd(OAc) ₂ (16.5 mg, 0.07 mmol), Cs ₂ C0 ₃ (600 mg, 1 .84 mmol), compound 25a (220 mg, 0.73 mmol) and piperidine (1 56 mg, 1.83 mmol) in toluene (8 mL) was stirred overnight at 80 °C under N ₂ . The reaction mixture was allowed to cool to RT and treated with satd. NH ₄ Q (aq. 10 mL). The resulting solution was extracted with EtO Ac (2 x 10 mL). The organic layers were combined and concentrated. The residue obtained was purified by flash chromatography

(EtO Ac/petroleum ether 1 :3) on silica gel to obtain compound 25b as a colorless oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for C ₁₇ H ₂₁ FN ₄ O ₃ : 349.2 (M+H); found: 349.1 , C. (l -(2-Fluoro-5-methoxyphenyl)-5-(piperidin-l-yl)-lH-l,2,3-tria zol-4-yl)methanol,

To a solution of compound 25b (110 nig, 0.32 mmol) in THF (4 mL), the solution of DIBAL-H in THF ^' (1 M, 1.5 niL) was added at -70 °C. The resulting solution was stirred for 1 h at -70 °C and the reaction was then quenched with satd. seignette salt solution (aq. 10 mL). The resulting solution was extracted with EtO Ac (2 x 10 mL). The organic layers were combined and concentrated. The residue obtained was purified by flash

chromatography (0-50% EtO Ac/petroleum ether) on silica gel to obtain compound 25c as a colorless oil. Mass Spectrum (i CMS. ESI pos.): Calcd. for C15H19FN4O2: 307.1 (M+H); found; 307.1.

D. (3 S)-methyl 3-cyclopropyl-3-(3-(( 1 -(2-fluoro-5-methoxyphenyl)-5-(piperidin- 1 -yl)- 1 H- 1 ,2,3-triazol-4-yl)methoxy)phenyl)propanoate, 25d

To a stirring solution of compound 25c (60 mg, 0.20 mmol), methyl (35)-3-cyclopropy!-3- (3-hydroxyphenyl)propanoate (64 mg, 0.29 mmol), Ph ₃ P (154 mg, 0.59 mmol) in THF (2.5 mL) was added a solution of di-terr-butyl azodicarboxylate (113 mg, 0.49 mmol) in THF (1 mL). The resulting solution was stirred for 3 h at RT and concentrated. The residue obtained was purified by flash chromatography (EtO Ac/petroleum ether 1 :2) on silica gel to obtain compound 25d as a colorless oil. Mass Spectrum (LCMS, ESI pos.): Caicd. for C28H33FN4O4: 509.2 (M 1 1 ); found: 509.3.

E. (3^)-3-Cyclopropyl-3-(3-((l-(2-fluoro-5-methoxyphenyl)-5-(pi peridin-l-yl)-lH- l,2,3-triazol-4-yl)methoxy)phenyl)propanoic acid, Cpd 44

Compound 44 was prepared from compound 25d following the procedure described in Example 3, Step I I. ^J H-NMR (300 MHz, DMSO-d ₆ ) δ (ppm): 7.47 (t, J= 9.3 Hz, 1H), 7.16 - 7.29 (m, 3H), 6.85 - 6.95 (m, 3H), 5.15 (s, 2H), 3.80 (s, 3H), 2.80 - 2.98 (m, 4H), 2.61 ·· 2.63 (m, 2H), 2.26 ·· 2.32 (m, i l l). 1.35 - 1.50 (m, 6H), 1.00 - 1.04 (m, 1H), 0.42 - 0.48 (m, 1H), 0.21 - 0,38 (m, 2H), 0.11 - 0, 18 (m, 1H). Mass Spectrum (LCMS, ESI pos.): Caicd. for C27H31FN4O4: 495.2 (M i l): found: 495.2.

Following the procedures described in Example 25 above, and substituting suitably selected and substituted reagents, starting materials and conditions as would be readily apparent to those skilled in the art, the following representative compounds of the present invention were prepared.

Cp ! No. Characterization

( _i S ^' )-3-Cyclopropyl-3-(3-((l -(2-fluoro-5-methoxyphenyl)-5- morpholino-lH-pyrazol-4-yl)raethoxy)phenyi)propanoic acid 'H-NMR. (400 MHz, DMSO- ) δ (ppm); 7.72 (s, 1H), 7,35 - 7.37 (m, 1H), 7.21 - 7.23 (m, i l l ). 7.06 - 7.19 [ in. 21 1 ). 6.84 - 6.92 (in,

73 3H), 4.99 (s, 2H), 3.79 (s, 3H), 3.43 - 3.44 (m, 4H), 2.91 - 2.97 (m, 4H), 2.58 - 2.62 (m, 2H), 2.26 - 2.28 (m , H), 0.99 - 1.01 (m, H), 0.48 - 0.50 (m, IH), 0.23 - 0.31 (m, 2H), 0.09 - 0.13 (m, IH).

Mass Spectrum (LCMS, ESI pos.): Calcd. for C -I ! -,iJ =0 . 496.2 (M+H); found: 496.3.

(5 -3-Cyclopropyl-3-(3-((l -(2-fluoro-5-methoxyphenyl)-5- morpholino-lH- 1 ,2, 3-triazol-4-yl)methoxy)phenyl)propanoic acid

¹ H-NMR (300 MHz, OMSO-d ₆ ) δ (ppm): 7.41 - 7.49 (m, IH), 7.25 - 7.33 (m, I H), 7.21 - 7.29 (m, 2H), 6.81 - 6.95 (m, 3H),

80 5.19 (s, 2H), 3.81 (s, 3H), 3.41 - 3.52 (m, 4H), 2.91 - 3.05 (m, 4H), 2.55 - 2.68 (m, 2H), 2.25 - 2.41 (in. IH), 0.91 - 1.01 (m, IH), 0.45 - 0.55 (m, IH), 0.21 - 0.38 (m, 2H), 0.05 - 0.12 (m, IH).

Mass Spectrum (LCMS, ESI pos.): Calcd. for C26H29FN4O5: 497.2 (M-i-H); found: 497.3.

Example 26

(3 -3-Cyclopropyl-3-(3-((5-(2-fluoro-5-methoxyphenyl)-4-(l,l, l-trifluoropropan-2- yl)thioph -3-yl)methoxy)phenyl)propanoic acid (Cpd 19)

A. Methyl f5')-3-(3-((4-bromo-5-(2-fluoro-5-methoxyphenyl)thiophen-3- yl)methoxy)phenyl)-3-cyclopropylpropanoate, 26a

A mixture of compound lOe (600 mg, 1.15 mmol), (2-fluoro-5-methoxyphenyl)boronie acid (215 mg, 1.27 mmol), Pd(dppf)Cl ₂ (42 mg, 0.06 mmol) and Cs ₂ C0 ₃ (751 mg, 2.30 mmol) in 1 ,4-dioxane (8 mL) and water (2 ml.) was stirred for 2 h at 85 °C under N ₂ in an oil bath. The reaction mixture was allowed to cool to rt and treated with NH ₄ CI (aq, 5 mL). The resulting mixture was extracted with EtOAc (3 x 5 mL). The organic layers were combined, dried over anhydrous sodium sulfate and concentrated. The resulting residue was purified by flash column chromatography (0-10 % EtO Ac/petroleum ether) on silica gel to obtain compound 26a yellow oil. ^l R NMR (300 MHz, OMSO-d ₆ ) δ (ppm): 7.99 (s, 1H), 7.20 - 7.34 (m, 2H), 7.02 - 7.10 (m, 2H), 6.85 - 6.96 (m, 3H), 5.04 (s, 2H), 3.79 (s, 3H), 3.51 (s, 3H), 2.73 ·· 2.77 (m, 2H), 2.25 - 2.83 (m, IH), 0.98 - 1.01 (m, 1H), 0.51 - 0.54 (m, IH), 0.38 - 0.41 (m, IH), 0.12 - 0.28 (m, 2H).

B . Methyl (S)-3 -cyclopropyl-3 -(3 -((5 -(2-fluoro- 5 -methoxypheny l)-4-(3 ,3 , 3 - trifluoroprop- 1 -en-2-yl)thiophen-3 -yl)methoxy)phenyl)propanoate, 26b

A mixture of compound 26b (280 mg, 0.54 mmol), 6-hydroxyhexyl hydrogen (3,3,3- trifluoroprop-l-en-2-yl)boronate (200 mg, 0.83 mmol), Pd(dppf)Cl ₂ (20 mg, 0.03 mmol), and CS2CO3 (438 mg, 1.34 mmol) in 1,4-dioxane (4 mL) and water (1 mL) was stirred for 2 h at 85 °C in an oil bath under N ₂ . The reaction mixture was allowed to cool to rt and treated with water (5 mL). The resulting mixture was extracted with EtO Ac (3 x 5 mL). The organic layers were combined, dried over anhydrous sodium sulfate and concentrated. The resulting residue was purified by flash chromatography (0-15 % EtO Ac/petroleum ether) on silica gel to obtain compound 26b as a yellow oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for 535.2 (M+H); found: 535.0.

C. Methyl (35 -3-cyclopropyl-3-(3-((5-(2-fluoro-5-methoxyphenyl)-4-(l,l,l- trifluoropropan-2-yl)thiophen-3-yl)methoxy)phenyl)propanoate , 26c

Into a 30-mL pressure tank reactor was placed compound 26b (230 mg, 0.43 mmol), ethyl acetate (10 mL) and Pd/C (10%, 200 mg). The resulting mixture was stirred overnight at 30 °C under a H ₂ atmosphere (3 atm). The mixture was filtered through a diatomaceous earth pellet and the filtrate was concentrated under reduced pressure. The resulting residue was purified by flash chromatography (0-5 % EtO Ac/petroleum ether) on si ca gel to obtain compound 26c as a yellow oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for C28H2SF4O4S: 537.2 (M+H); found: 537.2. D. (3 V3-Cyclopropyl-3-(3-((5-(2-fluoro-5-methoxyphenyl)-4-(l,l ,l-trifluoropropan-

2-yl)thiophen-3-yl)meihoxy)phenyl)propanoic acid, Cpd 19

A mixture of compound 26c (120 mg, 0.22 mmol) and LiOH.H ₂ 0 (92 mg, 2.19 mmol) in THF (4 mL), water (1 mL) and methanol (1 mL) was stirred overnight at rt. The resulting mixture was concentrated under reduced pressure. The residue was diluted with H ₂ 0 (5 mL) and was adjusted to pH~5 with HCi (1 N). The precipitates were collected by filtration. The crude solid (60 mg) were further purified by Prep-HPLC (75% MeCN H ₂ 0 to 95% MeCN/H ₂ 0 over 14 min). Compound 19 was obtained as a yellow solid. ¾ NMR (300 MHz, DMSO-ί β) δ (ppm): 12.01 (brs, IH), 7.83 (s, IH), 7.20 - 7.30 (m, 2H), 7.06 - 7. (m, IH), 6.86 - 6.93 (m, 4H), 5.10 (s, 2H), 3.75 - 3,80 (m, 4H), 2.64 - 2.68 (m, 21 1 ). 2.26 - 2,29 (m, IH), 1.37 (d, ./ 7.2 Hz, 3H), 0.93 - 1.02 (m, IH), 0.43 - 0.56 (m, H), 0.20 - 0.35 (m, 2H), 0.05 - 0.15 (m, H). Mass Spectrum (LCMS, ESI pos.): Calcd. for C27H26F4O4S: 545.2 (M+Na); found: 545,0.

Example 27

(35)-3-(3-((2-Chloro-5-(2-fluoro-5-methoxyphenyl)-4-(l ,1,1 -trifluoropropan-2- yl)thiophen-3-yl methoxy)phenyl)-3-cyclopropylpropanoic acid (Cpd 29)

A. Methyl (35)-3-(3-((2-chloro-5-(2-fluoro-5-methoxyphenyl)-4-(l ,1,1- trifluoropropan-2-yl)thiophen-3-yl)methoxy)phenyl)-3-cyclopr opylpropanoate, 27a

A mixture of compound 26c (60 mg, 0.11 mmoi) and NCS (15.8 mg, 0.12 mmol) in DMF (2 mL) was stirred overnight at rt. The reaction was quenched with water (10 mL). The mixture was extracted with DCM (3 x 5 mL). The organic layers were combined, washed with water (5 x 3 mL), dried over anhydrous sodium sulfate and concentrated. The resulting residue was purified by flash column chromatography (0-10 % EtO Ac/petroleum ether) on silica gel to obtain compound 27a as a yellow oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for C28H27CIF4O4S: 571.1 (M+H); found: 571.0.

B. (35)-3-(3-((2-Chloro-5-(2-fluoro-5-methoxyphenyl)-4-(l ,l,l -trifluoropropan-2- yl)thiophen-3-yl)methoxy)phenyl)-3-cyclopropylpropanoic acid, Cpd 29

Compound 29 was prepared from compound 27a following a similar procedure to that described in Example 26, Step D. 1H-NMR (300 MHz, OMSO-d ₆ ) δ (ppm): 7,84 (s, IH), 7.30 - 7,34 (nr. IH), 7.24 - 7.27 (m, IH), 7.15 - 7.16 (m, i l l ). 7.06 - 7.1 1 (m, IH), 6.89 - 6.95 (m, 2H), 5.13 (s, 2H), 3.77 (s, 3H), 3.30 - 3,33 (m, IH), 2.88 - 2.91 (m, IH), 2.67 - 2.69 (m, 21 1 ). 1.35 - 1.38 (111, 3H), 1.09 - 1 .14 (m, IH), 0,50 - 0.52 (m, IH), 0,23 - 0.35 (m, 2H), 0.06 - 0, 1 5 (nr. IH). Mass Spectrum (LCMS, ESI pos.): Calcd. for C27H25CIF4O4S: 573.1 [M+ H ₄ ] ⁺ ; found: 573.8.

Example 28 3-(5-Chloro-2-((4-(5,5-dimethylcyclopent-l-en-l -yl)-5-(2-fluoro-5- methoxyphenyl)isoxazol-3-yl)methoxy)pyridin-4-yl)-3-cyclopro pylpropanoic acid (Cpd 9)

5-Chloro-2-methoxyisonicotinic acid, 28a

A mixture of 2,5-dichloropyridine-4-carboxylic acid (5 g, 26 mmol) and sodium methylate (4.2 g, 77,8 mmol) in DMSO (20 mL) was stirred for 2 h at 100 °C in an oil bath. The reaction was allowed to cool to rt and treated with water (200 mL), and was adjusted to pH~3-4 with HCl (1 N). The resulting mixture was extracted with EtO Ac (3 x 200 mL). The combined organic layers were washed with water (2 x 50 mL), dried over anhydrous sodium sulfate and concentrated. This resulted in compound 28a as a light yellow powder. Mass Spectrum (I CMS. ESI pos.): Caicd. for ( ^' - ! Ι.,ΠΝί)-.: 186.0 (M-H); found: 186.0. B. 5-Chloro-N,2-dimethoxy-N-methylisonicotinamide, 28b

A mixture of compound 28a (1.4 g, 7.46 mmol), methoxy(methyl)amine hydrochloride (876 mg, 8.98 mmol), HATU (4.2 g, 11.05 mmol), and Et ₃ N (5 mL) in DCM (100 mL) was stirred for 2h at rt. The reaction was quenched with sodium bicarbonate (aq., 50 mL). The mixture was extracted with EtO Ac (3 x 200 mL). The combined organic layers were washed with water (2 x 100 mL), dried over anhydrous sodium sulfate and concentrated. The resulting residue was purified by flash column chromatography (0-20 %

EtO Ac/petroleum ether) on silica gel to obtain compound 28b as a light yellow oil. Mass Spectrum (LCMS, ESI pos.): Caicd. for GJ ! nCIX h: 231.0 (M+H); found: 231.0.

C. (5-Chloro-2-methoxypyridin-4-yl)(cyclopropyl)methanone, 28c

To a solution of compound 28b (3.4 g, 14.74 mmol) in THF (250 mL) was added bromo(cyclopropyl)magnesium (1M in THF, 29 mL, 29.48 mmol) at 0 °C under N ₂ . The resulting solution was stirred for 2 h at 0 °C. The reaction was then quenched with NH ₄ CI (satd, 100 mL). The mixture was extracted with EtO Ac (3 x 300 mL). The combined organic layers were washed with water (2 x 50 mL), dried over anhydrous sodium sulfate and concentrated. The resulting residue was purified by flash column chromatography (0- 20 % EtO Ac/petroleum ether) on silica gel to obtain compound 28c as a yellow oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for CIOHJ OC1N0 ₂ : 212.0 (M+H); found: 212.0.

D Methyl (E)-3-(5-chloro-2-methoxypyridin-4-yl)-3-cyclopropylacrylate , 28d

To a solution of methyl 2-(dimethoxyphosphoryl)acetate (1 0.3 g, 56.6 mmol) in THF (200 mL) was added sodium hydride (2.3 g, 56.6 mmol, 60% w/'w in mineral oil) in portions at 0 °C under N ₂ . The mixture was stirred for 30 mm at 0 °C followed by the addition of compound 28c (3.0 g, 14.2 mmol). The resulting solution was stirred for 3 h at 50 °C. The reaction was then quenched with NH4CI (satd, 00 mL). The mixture was extracted with EtO Ac (3 x 200 mL). The combined organic layers were washed with water (2 x 100 mL), dried over anhydrous sodium sulfate and concentrated. The resulting residue was purified by flash column chromatography (0-25 % EtO Ac/petroleum ether) on silica gel to obtain compound 28d as a light yellow oil Mass Spectrum (LCMS, ESI pos.): Calcd. for

( V;1 ! | (1N() ;: 268.1 (M+H); found: 268.1. E. Methyl 3-(5-chloro-2-methoxypyridin-4-yl)-3-cyclopropylpropanoate, 28e

A mixture of compound 28d (1.3 g, 4.85 mmol), NiCl ₂ ^' 6H ₂ 0 (1.0 g, 4.20 mmol) and NaBH ₄ (832 mg, 21.9 mmol) in methanol (100 mL) was stirred for Ih at 25 °C. The reaction was then quenched NH ₄ CI (satd, 50 mL). The mixture was extracted with EtOAc (3 x 100 mL). The combined organic layers were washed with water (2 x 50 mL), dried over anhydrous sodium sulfate and concentrated. The resulting residue was purified by flash column chromatography (0-10 % EtO Ac/petroleum ether) on silica gel to obtain compound 28d as a light yellow oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for Ci ₃ H ₁₆ ClN0 ₃ : 270.1 (M+H); found: 270.0.

F. 3-(5-Chloro-2-hydroxypyridin-4-yl)-3-cyclopropylpropanoic acid, 28f

To a solution of compound 28e (1.0 g, 3.71 mmol) in l,4-dioxane/H ₂ 0 (10 mL 1 : 1 v/v) was added cone. HCl (5 mL). The resulting mixture was stirred overnight at 100 °C. After cooling to rt, the mixture was extracted with EtOAc (3 x 10 mL). The organic layers were combined, dried over anhydrous sodium sulfate and concentrated. Compound 28f was obtained as a yellow oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for CnH^ONC : 242.1 (M+H); found: 242.0.

G. Ethyl 3-(5-chloro-2-hydroxypyridm-4-yl)-3-cyclopropylpropanoate, 28g

To a solution of compound 28f (1.2 g, 4.97 mmol) in ethanol (15 mL) was added cone. H ₂ SO ₄ (1 mL). The resulting mixture was stirred overnight at 80 °C. The reaction was allowed to cool to rt and treated with water (20 mL), and the mixture was adjusted to pH~4 with Na ₂ HC0 ₃ (aq., 1 N). The resulting mixture was extracted with EtOAc (3 x 50 mL). The organic layers were combined, dried over anhydrous sodium sulfate and concentrated. The resulting residue was purified by flash column chromatography (0-20 %

EtO Ac/petroleum ether) on silica gel to obtain compound 28g as a light yellow oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for (Υ,Ι Ι ,,,ΠΝΌ;: 270.1 (M+H); found: 270.0.

H. (4-(5,5-Dimethylcyclopent-l-en-l-yl)-5-(2-fluoro-5-methoxyph enyl)isoxazol-3- yl)methanoi, 28h

A mixture of compound 4e (1.5 g, 4.97 mmol), 2-(5,5-dimethylcyclopent-l-enyl)-4,4,5,5- tetramethyl-l,3,2-dioxaborolane (2.5 g, 11.3 mmol), Pd(dppf)Cl ₂ (183 mg, 0.25 mmol) and CS2CO ₃ (3.25 g, 9.97 mmol) in 1,4-dioxane (20 mL) and water (5 mL) was stirred overnight at 80 °C in an oil bath. After cooling to rt, the reaction was quenched with water (100 mL). The resulting mixture was extracted with EtOAc (3 x 100 mL). The organic layers were combined, dried over anhydrous sodium sulfate and concentrated. The resulting residue was purified by flash column chromatography (10-20 %

EtO Ac/petroleum ether) on silica gel to obtain compound 28h as a red oil. Mass Spectrum (LCMS, ESI pos,): Calcd. for C _; h.F 318.1 (M+H); found: 31 8.1. I. Ethyl 3-(5-chloro-2-((4-(5,5-dimethylcyclopent-l-en-l-yl)-5-(2-flu oro-5- methoxyphenyl)isoxazol-3-yl)methoxy)pyridin-4-yl)-3-cyclopro pylpropanoate, 28i

A mixture of compound 28g (129 mg, 0.48 mmoi), compound 28h (150 mg, 0.47 mmoi), w-Bu ₃ P (10% in hexane w/w, 2.5 g, 1.24 mmoi), and ADDP (309 mg, 1.23 mmoi) in toluene (5 mL) was stirred overnight at 60 °C. The reaction mixture was concentrated under reduced pressure and the resulting residue was purified by flash column

chromatography (0-20 % EtO Ac/petroleum ether) on silica gel to obtain compound 28i as a light yellow oil. Mass Spectrum ( I . CMS.. ESI pos,): Calcd. for C31H34CIFN2O5: 569.2 (M+H); found: 569.0.

J. 3-(5-Chloro-2-((4-(5,5-dimethylcyclopent-l -en-l-yl)-5-(2-fluoro-5- methoxyphenyl)isoxazol-3-yl)methox>')pyridin-4-yl)-3-cycl opropylpropanoic acid, Cpd 9

A mixture of compound 28i (100 mg, 0.18 mmoi) and LiOH.H ₂ 0 (17 mg, 0.71 mmoi) in THF (4 mL), water (1 mL) and ethanol (1 mL) was stirred overnight at 30 °C. The resulting mixture was concentrated under reduced pressure. The residue was diluted with water (5 mL) and the mixture was adjusted to pH~4 with HC1 (1 N). The resulting mixture was extracted with EtO Ac (3 x 10 mL). The organic layers were combined, dried over anhydrous sodium sulfate and concentrated. The resulting residue was purified by flash column chromatography (0-50 % EtO Ac/petroleum ether) on silica gel to obtain compound 9 as a white solid. ^l H (400 MHz, DMSG-i¾) δ (ppm): 8.17 (s, I H), 7.29 - 7.34 (m, IH), 7.11 - 7, 1 5 (nr. 2H), 7.03 (s, IH), 5.89 (s, IH), 5.31 - 5.39 (m, 21 1 ). 3.75 (s, 3H), 2.62 - 2.86 (m, 3H), 2.26 - 2,30 (m, 2H), 1.64 - 1 ,67 (m, 2H), 1.06 - 1.08 (m, IH), 0.70 (s, 6H), 0.40 - 0.60 (in, 1H), 0.25 - 0.35 (m, 2H), 0.10 - 0.15 (m, IH). Mass Spectrum (LCMS, ESI pos.): Calcd. for C29H30CIFN2O5: 541.2 (M+H); found: 541.2.

Following the procedures described in Example 28, and substituting suitably selected reagents, starting materials and conditions as would be readily apparent to those skilled in the art, the following representative compounds of the present invention were

Cpd No. Characterization

3-(5-Chloro-2-((5-(5,5-dimethylcyclopent-l-en-l -yl)-l -(2- fluoro-5-methoxyphenyl)-lH- 1 ,2,3-triazol-4- yl)methoxy)pyridin-4-yl)-3-cyclopropylpropanoic ac d

1H-NMR (400 Mi l/. DMSO~i -) δ (ppm): 8.17 (s, 1 H), 7.15 (t, J = 9.2 Hz, 1H), 7.02 -6.98 (m, I H), 6,93 - 6.92 (m, 2H), 5,99 (s,

78 IH), 5.38 (s, 2H), 3.80 (s, 3H), 2.88 - 2,74 (m, 3H), 2,42 - 2.34

(m, 211), 1.73 (t, J = 6.8 Hz, 2H), 1.05 - 1.09 (m, IH), 0.78 (s, 6H), 0.68 - 0.64 (m, IH), 0.49 - 0.45 (m, IH), 0.39 - 0.36 (m, IH), 0.21 - 0.23 (in. 1 1 1 ).

Mass Spectrum (LCMS, ESI pos.): Calcd. for C28H30CIFN4O4:

541.2 (M+H); found: 541.1.

Examp] e 29 3 -(5-Chloro-2-((5-(5 ,5-dimethylcyclopent- 1 -en- 1 -yl)- 1 -(2-fluoro-5- methoxyphenyl)-l H-pyrazol-4-yl)methoxy)pyridin-4-yl)-3-cyclopropylpropanoic acid

4-(Bromomethyl)-5-(5,5-dimethylcyclopent-l-en-l-yl)-l -(2-fluoro-5- methoxyphenyl)- 1 H-

29a To a solution of compound le (90 mg, 0.28 mmol) in DCM (10 mL) was added PBr ₃ (232 mg, 0.86 mmol) dropwise with stirring at 0 °C, followed by dropwise addition of DMF (0.05 mL). The resulting solution was stirred for 1 h at rt. The reaction was quenched with water (10 mL). The resulting mixture was extracted with DCM (3 10 mL), and the organic layers were combined, dried over anhydrous sodium sulfate and concentrated under reduced pressure. This resulted in compound 29a as a yellow oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for C _; xi hJkF ) _. 379.1 (M+H); found: 379.0.

B. Ethyl 3-(5-chloro-2-((5-(5,5-dimethylcyclopent-l-en-l-yl)-l -(2-fluoro-5- methox henyl)-lH-pyrazol-4-yl)methox>')pyridin-4-yl)-3-cycloprop ylpropanoate,

A mixture of compound 29a (100 mg, 0.26 mmol), compound 28g (142 mg, 0.53 mmol), and Ag ₂ C0 ₃ (72 mg) in toluene (5 mL) was stirred for 2 h at 50 °C. The reaction was then quenched with water (20 mL). The resulting mixture was extracted with EtOAc (3 x 10 mL) and the organic layers were combined, dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by flash column chromatography (0-10 % EtO Ac/petroleum ether) on silica gel to obtain compound 29b as a colorless oil. 1H-NMR (400 MHz, OMSO-dg) δ (ppm): 8.21 (s, 1H), 7.88 (s, IH), 7.32 (t, J = 9.2 Hz, IH), 7.00 - 7.06 (m, 3H), 6.01 (s, 1 1 1 ). 5.09 (s, 2H), 3.93 - 3.98 (m, 2H), 3.74 (s, 3H), 2.75 - 2.87 (m, 3H), 2.30 - 2.34 (m, 2H), 1.61 - 1.63 (m, 2H), 1.05 - 1.15 (m, 4H), 0.66 (s, 6H), 0.55 - 0.65 (m, I H), 0.48 - 0.53 (m, IH), 0.28 - 0.35 (m, IH), 0.15 - 0.21 (m, I H). C. 3-(5-Chloro-2-((5-(5,5-dimethylcyclopent-l-en-l -yl)-l -(2-fluoro-5- methoxyphenyl)-lH-pyrazol-4-yl)methoxy)pyridin-4-yl)-3-cyclo propylpropanoic acid, Cpd 71

A mixture of compound 29b (70 mg, 0.12 mmol) and LiOH.HiQ (52 mg, 1.24 mmol) in THF (4 mL), ethanol (1 mL) and water (1 mL) was stirred overnight at rt. The resulting mixture was concentrated under reduced pressure. Water (4 mL) was added, and the mixture was adjusted to pH ~ 5-6 with HC1 (2 N). The precipitates were collected by filtration to yield compound 71 white solid. H-NMR (400 MHz, CD ₃ OD) δ (ppm): 8.10 (s, 1H), 7.86 (s, IH), 7.18 (t, J= 7.2 Hz, 1H), 7.01 - 7.06 (m, 1H), 6.93 - 6.98 (m, I I I ). 6.88 (s, H i ). 6.00 (s, i l l ). 5.15 (s, 2H), 3.77 (s, 3H), 2.85 - 2.95 (m, i l l ). 6.68 - 6.81 (m, 2H), 2.40 (m, 2H), 1.71 (t, ,/ 7,2 Hz, 2H), 1.05 - 1.15 (m, IH), 0,76 (s, 6H), 0, 55 - 0.65 (m, IH), 0.48 - 0,53 (m, IH), 0.28 - 0,35 (m, IH), 0.15 - 0.21 (m, IH). Mass

Spectrum (LCMS, ESI pos.): Calcd. for C ₂₉ H ₃₁ CIFN ₂ O ₄ : 538,2 [M-H] ^' ; found: 538,2.

Following the procedures described in Example 29 and substituting suitably selected reagents, starting materials and conditions as would be readily apparent to those skilled in the art, the following representative compound of the present invention were prepared.

3-Cyclopropyl-3-(2-((5-(5,5-dimethylcyclopent-l-en-l -yl)-l-(2- fluoro-5-methoxyphenyl)-lH-pyrazol-4-yl)methoxy)pyridin-4- yljpropanoic acid

1H-NMR (400 MHz, DMSO-iij) δ (ppm): 8.05 (d, J = 5.2 Hz,

IH), 7.86 (s, IH), 7.16 - 7,20 (m, IH), 6.91 - 7,04 (m, 3H), 6.70 (s, IH), 6,00 (s, IH), 5.15 (s, 2H), 3.77 (s, 3H), 2.68 - 2,79 (m,

2H), 2.28 - 2.40 (111, 3H), 1.69 (t, ,/ 6,8 Hz, 21 1 ). 0.98 - 1.08

(m, IH), 0.76 (s, 6H), 0.55 - 0.65 (m, IH), 0.48 - 0.53 (111, H), 0.28 - 0.35 (111, H), 0.10 - 0.20 (m, IH),

Mass Spectrum (LCMS, ESI pos,): Calcd, for C ₂₉ H ₃₂ FN ₃ O ₄ :

504.2 [M-H] ^" ; found: 504.1. Example 303-Cyclopropyl-3-(2-((5-(5,5-dimethylcyclopent-l -en-l -yl)-l-(2-fluoro-5- methoxyphenyl)-lH-l , -triazol-4-yl)methoxy)pyridin-4-yl)propanoic acid (Cpd 63)

A. (5-(5, 5-Dimethylcyclopent- -en- 1 -yl)- 1 -(2-fluoro- 5-methoxyphenyl)- 1 Η- 1 ,2,3- triazol-4-yl)methanol, 3

Compound 30a was prepared from compound 5d following similar procedures as described in Example 6, Steps A, B and D. Mass Spectrum (LCMS, ESI pos.): Calcd. for CVH;,,FN i 318. (M+H); found: 318.0.

Ethyl 3-cyclopropyl-3-(2-hydroxypyridin-4-yl)propanoate,

Compound 30b was prepared following similar procedures as described in Example 2 Steps A-F. Mass Spectrum (LCMS, ESI pos.): Calcd. for C ₁₃ H ₁₇ N0 ₃ : 236.1 (M+H) ⁺ , found: 236.1. C. 3-Cyclopropyl-3-(2-((5-(5,5-dimethylcyclopent-l -en-1 -yl)- 1 -(2-fluoro-5- methoxyphenyl)-lH-l,2,3-triazol-4-yl)methox>')pyridin-4-y l)propanoic acid, Cpd 63

Compound 63 was prepared from compound 30a and 30b following procedures similar to those described in Example 29, Steps A-C. 1H-NMR (400 MHz, CDCI3) δ (ppm): 8.08 (s, 1 1 1 ). 7.31 (t, 9.2 Hz, I I I ). 7.16 - 7.19 (m, IH), 7.10 - 7.12 (in. i l l ). 6.98 (d, J = 1.2 Hz, 1 H) , 6.75 (s, 1H), 6.07 (s, 1H), 5.34 (s, 2H), 3.83 (s, 3H), 2.76 - 2.80 (m, 2H), 2.40 - 2.44 (m, 2H), 2.31 - 2.34 (m, IH), 1.75 (t, 7.2 Hz, 2H), 1.06 - 1.08 (m, ! ! ! }. 0.82 (s, 6H), 0.62 - 0.66 (m, IH), 0.45 - 0.46 (m, IH), 0.34 - 0.38 (m, I H), 0.21 (m, IH). Mass

Spectrum (LCMS, ESI pos.): Calcd. for C28H31FN4O4: 505.2 [M-H] ^" ; found: 505.2.

D. (5 -3-Cyclopropyl-3-(2-((5-(5,5-dimethylcyclopent-l-en-l-yl)-l -(2-fluoro-5- methoxyphenyl)-lH-l,2,3-triazol-4-yl)methoxy)pyridin-4-yl)pr opanoic acid, Cpd 64

E. and

(i?)-3-cyclopropyl-3-(2-((5-(5,5-dimethylcyclopent-l -en-l-yl)-l -(2-fluoro-5- methoxyphenyl)-lH-l,2,3-triazol-4-yl)methoxy)pyridin-4-yl)pr opanoic acid, Cpd

Compounds 64 and 75 were obtained through a chiral HPLC separation of the racemic compound 63, using the following conditions: column, (R,R)WHELK-01 5/100 Kromasil, 25cm*21.1mm; mobile phase, HEX( contained 0.1% HAc) and ethanol (hold 20.0% ethanol in 19 mm); detector, UV 254/220nm.

Compound 64. ¹ H-NMR (400 MHz, DMSQ-i¾) δ (ppm): 8.08 (d, J = 5.2 Hz ,1H), 7.44 (t, J 9.2 Hz, IH), 7.15 - 7.25 (m, 11 1 ). 6.97 (d, ./ 4.8 Hz, I H), 6.73 (s, I H), 6.08 (s, IH), 5.29 (s, 2H), 3.77 (s, 3H), 2,67 - 2.69 (m, 2H), 2.22 - 2.38 (m, 3H), 1.64 (t, J 7.2 Hz, 2H), 0.93 - 1.01 (m, I I I ). 0.71 (s, 6H), 0.43 - 0.51 (m, IH), 0.21 - 0.32 (in. 2H), 0.11 - 0.18 (m, IH). Mass Spectrum (LCMS, ESI pos.): Calcd. for _s I l NjiXj: 505.2 [M-H] ^" ; found: 505.2.

Compound 75. 1H-NMR (400 MHz, OMSO-dg) δ (ppm): 12.05 - 12.15 (br.m, IH),

8.07 (s, IH), 7.44 (t, J = 9.2 Hz, IH), 7.15 - 7.25 (m, 2H), 6.97 (m, IH), 6.72 (s, IH), 6.08 (s, IH), 5.29 (s, 2H), 3.77 (s, 3H), 2.67 - 2.69 (m, 2H), 2.22 - 2.38 (m, 3H), 1.64 (t, J = 7.2 Hz, 2H), 0.93 ·· 1.01 (m, IH), 0.71 (s, 6H), 0.43 - 0.51 (m, IH), 0.21 - 0.32 (m, 2H), 0.11 - 0.18 (m, IH). Mass Spectrum (LCMS, ESI pos.): Calcd. for C ₂ 8¾iFN ₄ 0 ₄ : 505.2 [M-H] ^" ; found: 505.2.

Example 31

(5)-3-Cyclopropyl-3-(3-((l-(2-fluoro-5-methoxyphenyl)-5-isob utyl-lH- ^'

yl)methoxy)phenyl)propanoic acid (Cpd 93)

Into a 25-mL pressure tank reactor, was placed compound 91 (60 ig, 0.13 mmol), EtOAc (2 mL) and Pd/C (10%, 70 mg). The resulting mixture was stirred for 1 day at rt under a H ₂ atmosphere (3.5 atm). The mixture was filtered through a diatomaceous earth pellet and the filtrate was concentrated under reduced pressure. This resulted in compound 93 as a white solid. ^l H-NMR (400 MHz, DMSO-ifc) δ (ppm): 11.42 - 12.13 (br.m, IH), 7.74 (s, IH), 7.42 - 7,43 (m, IH), 7.14 - 7,21 (m, 3H), 6.82 - 6.92 (m, 3H), 4.93 (s, 2H), 3.79 (s, 3H), 2.61 - 2.65 (m, 3H), 2,49 - 2.51 (m, I H), 2,26 - 2,31 (m, IH), 1.32 - 1.34 (m, IH), 0.92 - 1 .12 (m, I H), 0,68 (d, ,/ 3.3 Hz, 6H), 1 ,41 - 1.50 Or, IH), 0.04 - 0,31 (m, 3H). Mass Spectrum (LCMS, ESI pos.): Calcd. for (V! h , I ^: V0 . 467.2 j M H | . found: 467.2. Example 32

(iS)-3-Cyclopropyl-3-(3-((5-cyclopropyl-l-(5-fluoro-2-methox ypyridin-4-yl)-lH-pyrazol-4- yl)methoxy)phenyl)propanoic acid (Cpd 70)

and

(»S)-3-cyclopropyl-3-(3-((l-(5-fluoro-2-methoxypyridin-4-yl )-lH-pyrazol-4- yl)methoxy)phenyl)propanoic acid (Cpd 81)

Cpd 70

Ethyl 5-cyclopropyl-l -(5-fluoro-2-methoxypyridin-4-yl)-lH-pyrazole-4- carboxylate, 32a, and ethyl l -(5-fluoro-2-methoxypyridin-4-yl)-l.H-pyrazol carboxylat '

Compound 32a was prepared following procedures similar those described in Example 1, Steps A-D. Mass Spectrum (LCMS, ESI pos.): Calcd. for < <ί ! |.,l V,0 :. 306.1 (M+H); found: 306.1.

Compound 32a' was the de-bromo bi-product of the Suzuki reaction leading to compound 32a. Mass Spectrum (LCMS, ESI pos.): Calcd. for C ₁₂ H ₁₂ FN303 _: 266.1 (M+H); found: 266.0. Compounds 32a and 32a'were used in the subsequent reaction as a mixture, without further purification.

B. Methyl (5)-3-cyclopropyl-3-(3-((5-cyclopropyl-l-(5-fluoro-2-methoxy pyridin-4- yl)-lH-pyrazol-4-yl)methoxy)phenyl)propanoate, 32b, and methyl (5)-3- cyclopropyl-3-(3-((l -(5-fluoro-2-methoxypyridin-4-yl)-lH-pyrazol-4- yl)methoxy)phenyl)propanoate, 32b'

Compounds 32b and 32b' were prepared following procedures similar to those described in Example 3, Steps E-G. The crude mixture was purified by flash column

chromatography (0-10 % EtO Ac/petroleum ether) on silica gel to give compound 32b as a yellow oil and compound 32b' as a yellow oil.

Compound 32b: Mass Spectrum (LCMS, ESI pos.): Calcd. for (\J !¾FN ;().; _. 466.2 (M+H); found: 466.0.

Compound 32b': Mass Spectrum (LCMS, ESI pos.): Calcd. for C23H24FN3O4: 426.2 (M+H); found: 425.9.

C. (5 -3-Cyclopropyl-3-(3-((5-cyclopropyl-l-(5-fluoro-2-methoxypyr idin-4-yl)-lH- pyrazoi-4-yl)methoxy)phenyl)propanoie acid, Cpd 70

Compound 70 was prepared from compound 32b following a procedure similar to that described in Example 29, Step C. 1H-NMR (300 MHz, DMSO-<¾) δ (ppm): 8.42 (s, IH), 7.83 (s, H), 7.12 - 7.23 (m, 2H), 6.84 - 6.92 (m, 3H), 4.97 (s, 2H), 3.91 (s, 3H), 2.61 - 2.62 (m, 2H), 2.26 - 2.29 (m, IH), 1.88 - 1.90 (m, IH), 0.98 - 1.01 (m, IH), 0.75 - 0.82 (m, 2H), 0.47 ·· 0.55 (m, 3H), 0.22 - 0.32 (m, 2H), 0.1 1 - 0.14 (m, IH). Mass Spectrum (LCMS, ESI pos.): Calcd. for C25H26FN3O4: 452.2 [M+H]"; found: 452.2.

D. (5 -3-Cyclopropyl-3-(3-((l-(5-fluoro-2-methoxypyridin-4-yl)-lH- pyrazol-4- yl)methoxy)phenyl)propanoic acid, Cpd 81

Compound 81 was prepared from compound 32b' following a procedure similar to that described in Example 29, Step C. 1H-NMR (400 MHz, DMSO~ _> ) δ (ppm): 8.50 (d, J 2.0 Hz, I I I). 8.37 (d, J= 3.6 Hz, 1 1 1). 8.01 (s, ! ! i). 7.26 (d, J = 5.6 Hz, i l l ). 7.16 - 7.20 (m, 1H), 6.80 - 6.90 (m, 3H), 5.05 (s, 2H), 3.89 (s, 3H), 2.44 - 2,47 (in, 2H), 2.29 - 2.34 (m, IH), 0.94 - 0.96 (m, IH), 0.44 - 0.45 (m, 1H), 0.22 - 0.27 (m, 2H), 0.02 - 0.08 (m, 1H). Mass Spectrum (LCMS, ESI pos.): ( a led. for C22H22FN3O4: 412.2 [M+H] ⁺ : found: 412.1.

Example 33(E)-3-Cyclopropyl-3-(3-(2-(5-cyclopropyl-l-(2-fluoro-5-met hoxyphenyl)-lH- pyrazol-3-yl)vinyl)phenyl)propanoic acid (Cpd 76)

A. (5-Cyclopropyl-l-(2-fluoro-5-methoxyphenyl)-lH-pyrazol-4-yl) methanol, 33a

A mixture of compound 2a (3 g, 9.96 mmol), cyclopropylboronic acid (1.72 g, 20.0 mmol), K3PO4 (6.36 g, 30.0 mmol), Pd(OAc) ₂ (1 12 mg, 0.50 mmol) and PCy ₃ .HBF ₄ (368 mg, 1.00 mmol) in toluene (60 mL)/ H ₂ 0 (10 mL) was stirred for 2 h at 80 °C, After cooling to rt, the reaction was quenched with water (100 mL). The resulting mixture was extracted with EtO Ac (3 x 100 mL). The organic layers were combined, dried over anhydrous sodium sulfate and concentrated. The resulting residue was purified by flash columnchrornatography (0-40 % EtO Ac/petroleum ether) on silica gel to obtain compound 33a as a yellow solid. Mass Spectrum (LCMS, ESI pos.): Calcd. for C _M H _IS FK^: 263, 1 [M+Hf; found: 263.1 ,

B. 4-(Bromomethyl)-5-cyclopropyl-l -(2-fluoro-5-methoxyphenyl)-lH-pyrazole, 33b

Compound 33b was prepared from compound 33a following a similar procedure described in Example 29, Step A. Mass Spectrum (LCMS, ESI pos.): Caicd. for

Ci ₄ H _{i 4} BrFN ₂ 0: 327.0 [M+Hf ; found: 327.0.

C. ((5-Cyclopropyl-l-(2-fluoro-5-methoxyphenyl)-lH-pyrazol-4- yl)methyl)triphenylphosphonium, 33c

A mixture of compound 33b (1 g, 3.08 mmol) and PPh ₃ (800 mg, 3.05 mmol) in toluene (20 mL) was stirred for 6 h at 120 °C. The resulting mixture was concentrated under reduced pressure. The residue obtained was purified by flash column chromatography (0- 5% methanol/DCM) on silica gel to yield compound 33c as a gray solid. Mass Spectrum (LCMS, ESI pos.); Caicd. for ( H ^FN P: 507.2 [M] ⁺ ; found: 507,2,

Methyl 3-cyclopropyl-3- -(((trifluoromethyl)sulfonyl)oxy)phenyl)propanoate, 33d

To a solution of methyl 3-cyclopropyl-3-(3-hydroxyphenyl)propanoate (1.5 g, 6.82 mmol, methyl ester of compound lf-B) and tnethylamine (2.83 mL, 20.5 mmol) in DCM (20 mL) was added Tf ₂ 0 (2,9 g, 10.3 mmol) dropwise at 0 °C under N ₂ . The resulting solution was stirred for 4 h at 0 °C in a water/ice bath. The reaction was then quenched with brine (50 mL). The resulting solution was extracted with DCM (3 x 50 mL), and the organic layers were combined, dried over anhydrous sodium sulfate and concentrated under reduced pressure. The resulting residue was purified by flash column chromatography (0-20 % EtO Ac/petroleum ether) on silica gel to obtain compound 33d as a yellow oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for C14HJ 5F3O5S : 353.1 | M 1 1 ; ; found: 353.1.

E. Methyl 3-cyclopropyl-3-(3-formylphenyl)propanoate, 33e

A mixture of compound 33d (1.76 g, 5.00 mmol), triethylamine (1.26 g, 12.5 mmol), tnethyisilane (1.16 g, 10.0 mmol), Pd(OAc) ₂ (56 mg, 0.25 mmol) and DPPP (103 mg, 0.25 mmol) in DMF (25 mL) was stirred for 5 at 80 °C under a CO _(g) atmosphere (5 atm). After cooling to rt, the reaction was quenched with water (25 mL). The resulting mixture was extracted with EtO Ac (3 x 30 mL). The organic layers were combined, dried over anhydrous sodium sulfate and concentrated. The resulting residue was purified by flash column chromatography (0-15% EtO Ac/petroleum ether) on silica gel to obtain compound 33e as a yellow oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for C^H^Os: 233.1

[M+H] ⁺ ; found: 233.0.

F. Methyl (E)-3-cyclopropyl-3-(3-(2-(5-cyclopropyl-l -(2-fluoro-5-methoxyphenyl)- lH-pyrazol-4-yl)vinyl)phenyl)propanoate, 33f

To a stirring solution of compound 33c (587 mg, 1.00 mmol) in THF (14 mL) was added LDA (1 mL, 2M in THF) dropwise at -70 °C under N ₂ . After 30 mm at -70 °C, a solution of compound 33e (350 mg, 1.51 mmol) in THF (1 mL) was added dropwise. The resulting solution was stirred for 1 h at -70 °C. The reaction was then quenched with NH ₄ CI (satd 10 rnL). The resulting mixture was extracted with EtO Ac (3 x 20 mL). The organic layers were combined, dried over anhydrous sodium sulfate and concentrated. The resulting residue was purified by flash chromatography (0-15% EtO Ac/petroleum ether) on silica gel to obtain compound 33f as a yellow oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for C28H29FN2O3: 461.2 J M ! Π ; found: 461.3.

G. (E)-3-Cyclopropyl-3-(3-(2-(5-cyclopropyl-l-(2-fluoro-5-metho xyphenyl)-lH- pyrazol-3-yl)vinyl)phenyl)propanoic acid, Cpd 76

Compound 76 was prepared from compound 33f following a procedure similar to that described in Example 26, Step D. 1H-NMR (300 MHz, DMSO-i ₆ ) δ (ppm): 8.01 (s, 1H), 6.89 - 7.50 (m, 911), 3.80 (s, 3H), 2.59 - 2.79 (m, 2H), 2.31 (q, J = 7.9 Hz, 1H), 1.85 - 1.96 (m, 1H), 1.01 - 1.11 (m, 1H), 0.77 - 0.90 (m, 2H), 0.10 - 0.60 (m, 6H). Mass Spectrum (LCMS, ESI pos.): Calcd. for C27H27FN2O3: 447.2 [M+Hf; found: 447.2.

Following the procedures described in Example 33 above, and substituting suitably selected reagents, starting materials and conditions as would be readily apparent to those skilled in the art, the following representative compound of the present invention were prepared.

Cpd No. Characterization

(5',E)-3-Cyclopropyl-3-(3-(2-(4-cyclopropyl-3-(2-fluoro-5- methoxyphenyl)isoxazol-5-yl)vinyl)phenyl)propanoic acid

¾-NMR (400 MHz, DMSO-i/,5) δ (ppm): 7,62 (s, !!!}.7,56 (d. J = 7,6 Hz, 1H), 7.20 - 7,48 (m, 5H), 7.07 - 7.18 (m, 2H), 3.80

58 (s, 3H), 2,63 - 2.70 (m, 2H), 2,29 - 2,40 (m, 1H), 1,75 - 1,84 (m, HI), 1,01 - 1.12 (m, !!!}.0,76 - 0,86 Or, 211).0.48 - 0,56 (m, 1H), 0.23 - 0.37 (m, 4H), 0.12 - 0.18 (m, 1H).

Mass Spectrum (LCMS, ESI pos.): Calcd. for C- _. -f hJ- ^' NC :

448.2 |\! iil : found: 448.1.

(5,i^-3-Cyclopropyi-3-(3-(2-(5~cyclopropyl~l~(2-fluoro~5~ methoxyphenyl)-lH-l,2,3-triazol-4-yl)vinyl)phenyl)propanoic acid

¹ H-NMR (300 Mil:/ P SC /,) δ (ppm): 7, 16 - 7.52 (m, 9H),

74 3.82 (s, 3H), 2.73 (d, J= 12 Hz, 211).2.31 - 2.37 (m, 111).1,90 - 1.95 (m, 1H), 1.06 - l,09(m, HI), 0,88 - 0,95 (m, 2H), 0.54 - 0.57 (m, 3H), 0, 15 - 0.37 (m, 3H).

Mass Spectrum (LCMS, ESI pos,): Calcd, for (· .Π;,,Ι ^; Ν„()-,:

448.2 [M+H] ⁺ ; found: 448,3.

Example 34

-Cyclopropyl-3-(3-(2-(5-cyclopropyl-l-(2-fluoro-5-methoxy phenyl)-lH-pyrazol-3- yl)ethyl)phenyl)propanoic acid (Cpd 77)

Methyl 3-cyclopropyl-3-(3-(2-(5-cyclopropyl-l-(2-fluoro-5-methoxyph enyl)-lH- pyrazol-4-yl)ethyl)phenyl)propanoate, 34a

To the solution of compound 33f (120 mg, 0.26 mmol), in methanol (5 mL) was added Pd/C (10%, 30 mg). The resulting mixture was stirred for 2 h at rt under a H ₂ atmosphere (3 atm). The mixture was filtered through a diatomaceous earth pellet and the filtrate was concentrated under reduced pressure to provide compound 34a as colorless oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for (\. _χ Π; , Ι ^; νθ :: 463.2 [M+Hf; found: 463.3.

B. 3-Cyclopropyl-3-(3-(2-(5-cyclopropyl-l -(2-fluoro-5-methoxyphenyl)-lH-pyrazol- 3-yl)ethyl)phenyl)propanoic acid, Cpd 77

Compound 77 was prepared from compound 34a following a procedure similar to that described in Example 29, Step C. ¹ H-NMR (400 MHz, DMSO-<¾) δ (ppm): 7.50 (s, i l l).

7.34 { !. ./ 9.5 Hz, I I I ). 7,20 - 6.97 (m, 6H), 3.77 (s, 3H), 2.70 - 2,88 (m, 4H), 2.29 - 2,55

(m, 3H), 1 ,55 - 1.70 (m, i l l). 0.89 - 0.97 (m, 1 1 1 ). 0.57 - 0,70 (m, 2H), 0.41 - 0,47 (m, i l l).

0.18 - 0,27 (m, 4H), 0.01 - 0,09 (m, 1H). Mass Spectrum (LCMS, ESI pos.): Calcd. for C27H29FN2O3: 449.2 [M+Hf; found: 449.2.

Following the procedures described in Example 34 above, and substituting suitably selected reagents, starting materials and conditions as would be readily apparent to those skilled in the art, the following representative compound of the present invention were prepared.

Cpd No. Characterization

3-Cyclopropyl-3-(3-(2-(4-cyclopropyl-5-(2-fluoro-5- methoxyphenyl)isoxazol-3-yl)ethyl)phenyl)propanoic acid 1H-N R (400 MHz, DMSO-f/ ₆ ) δ (ppm): 7,33 (†, 1 10.0, 1.4 Hz, ill).7.05 - 7.25 (m, 6H), 3.80 (s, 3H), 3.01 (s, 411).2.51 -

7 2.59 ( m, 211).2.31 (q, ./ 7.8 Hz, ill).1.64 (tt, ,/ 8.3, 5.3 Hz, Hi).0.89 - 0,95 (m, ill).0.72 - 0.85 (m, 211).0.39 - 0.52 (m, 1H), 0.16 - 0.32 (m, 4H), 0,07 - 0.12 (m. ill).

Mass Spectrum (LCMS, ESI pos.): Calcd. for C I : _S F\i>,: 450.2 [M+Hf; found: 450.2.

(3^)-3-Cyclopropyl-3-(3-(2-(4-(2,2-dimethylcyclopentyl)-3-(2 - fluoro-5-methoxyphenyl)isoxazol-5-yl)ethyl)phenyl)propanoic

^! H-NMR(300MHz, CD ₃ OD) δ (ppm): 7.10 - 7.21 (m, 5H), 6.98 - 7.08 (m, 1H), 6.86 - 6.88 (m, 1H), 3.80 (s, 3H), 3.07 -

54

3.19 (m, 4H), 2.68 - 2.74 (ni, 2H), 2.32 - 2.46 (m, 2H), 1.57 - 1.77(m, 4H), 1.37 - 1.43 (ni, 2H), 1.01 - 1.09 (m, 1H), 0.59 - 0.64 (m, 711).0.29 - 0.37 (111, 2H), 0.11 - 0.13(m, ill). Mass Spectrum (LCMS, ESI pos.): Calcd. for (\|ί Ι-,,ΓΝΟ,: 506.3 [M+Hf; found: 506.2.

Cpd No. Characterization

(5)-3-Cyclopropyl-3-(3-(2-(4-cyclopropyl-3-(2-fluoro-5- methoxyphenyl)isoxazol-5-yl)ethyl)phenyl)propanoic acid

1H-NMR (400 MHz, f)MS()-< ,,5 δ (ppm): 7.30 (t, J = 9.3 1 1

H i). 7.18 (t, ./ 8 Hz, i l l). 6.98 - 7.12 (m. 5H), 3.78 (s, 3H),

57 3.10 (t, J= 7.3 Hz, 2H), 2.98 (t, J= 7.5 Hz, 2H), 2.51 - 2.62 (m, 3H), 2.21 - 2,38 (in, i l l). 1.36 - 1.44 (m, i l l). 0.86 - 0.97 (m, 1H), 0.53 - 0.63 (m, 2H), 0.41 - 0.48 (m, I I I). 0, 18 - 0.26 (m, 2H), 0.03 - 0,09 (m, 2H). Mass Spectrum (LCMS, ESI pos.):

Calcd. for (VI l- _s i ^; N0. ₄ - 450.2 [ H | : found: 450.1 ,

(<S)-3-Cyclopropyl-3-(3-(2-(5-cyclopropyl-l-(2-fluoro- 5- methoxyphenyl)- 1H- 1 ,2,3 -triazol-4-yl)ethyl)phenyl)propanoic

lH-NMR (300 MHz, DMSO-< ₆ ) δ (ppm): 7.46 (t, J= 9.4 Hz, 1H), 6.95 ·· 7.26 (m, 6H), 3.80 (s, 3H), 2.97 (d, ./ 4.3 Hz, 4H),

79

2.54 ·· 2.69 (m, 2H), 2.20 - 2.28 (m, i l l). 1.47 - 1.53 (m, 1H), 0.95 - 0.99 (m, 1H), 0.64 - 0.70 (m, 2H), 0.45 - 0.50 (m, 1H), 0.19 - 0,29 (m, 4H), 0.05 - 0.10 (m, i l l). Mass Spectrum (LCMS, ESI pos.): Calcd. for Ο,,Π^ΓΝ ()·.· 450,2 [M+Hf ;

found: 450.3.

Example 35

(5Ji)-3-Cyclopropyl-3-(3-(2-(4-(5,5-dimethylcyclopent-l-en-l -yl)-3-(2-fluoro-5- methoxyphenyl)isoxazol-5-yl)vinyl)phenyl)propanoic acid (Cpd 56) and (5,Z)-3-cyclopropyl-3-(3-(2-(4-(5,5-dimethylcyclopent-l-en-l -yl)-3-(2-fluoro-5- methoxyphenyl)isoxazol-5-yl)vinyl)phenyl)propanoic acid (Cpd 59)

A mixture of compound 7f (400 mg, 1.32 mmol), IBX (500 mg, 1.79 mmol) in DMSO (5 mL) was stirred overnight at 40 °C. The reaction was then quenched with NaHC0 ₃ (satd, 5 mL). The resulting solution was extracted with EtOAc (3 x 5 mL), and the organic layers were combined, washed with water (2 x 10 mL) and concentrated under reduced pressure. The residue was purified by flash column chromatography (0-20%

EtO Ac/petroleum ether) on silica gel to obtain compound 35a as a yellow oil. ¹ H-NMR (300 MHz, CDCI ₃ ) δ (ppm): 8.54 (s, i l l). 7.12 - 7.18 (m, I I I). 7.01 -7.04 (m, 2H), 3.83 (s, 3H).

4-(5,5-Dimethylcyclopent-l-en-l-yl)-3-(2-fluoro-5-methoxy phenyl)isoxazole-5- carbaldehyde, 35b

Compound 35b was prepared from compound 35a following a procedure similar described in Example 3, Step D. Mass Spectrum (LCMS, ESI pos.): Calcd. for

CisHjgFNCh 316.1 (M+H); found: 316.1. C. Methyl (5)-3-cyclopropyl-3-(3-(((trifluoromethyl)sulfonyl)oxy)pheny l)propanoate,

Compound 35c was prepared following a procedure similar to that described in Example 33, Step D. Compound 35c was used in the next step without further purification.

D. Methyl (5)-3-cyclopropyl-3-(3-(hydroxymethyl)phenyl)propanoate, 35d

A mixture of compound 35d (500 mg, 1.42 mmol), potassium acetoxymethyl- trifluoroborate (500 mg, 2.79 mmol), Pd ₂ (dba) ₃ (100 mg, 0.11 mmol), RuPhos (100 mg, 0.21 mmol) and Na ₂ C0 ₃ (200 mg, 1.90 mmol) in 1,4-dioxane (15 mL) and ¾0 (1.5 mL) was stirred for 3 h at 110 °C under N ₂ . The reaction was then quenched with water (100 mL). The resulting mixture was extracted with EtOAc (2 x 50 mL). The organic layers were combined, dried over anhydrous sodium sulfate and concentrated. The resulting residue was purified by flash column chromatography (0-50% EtO Ac/petroleum ether) on silica gel to obtain compound 35d as a yellow oil. Ή-NMR (300 MHz, CDC1 ₃ ) δ (ppm): 7.15 - 7.30 (in, 4H), 4,69 (s, 2H), 3,60 (s, 3H), 2,68 - 2.80 (m, 2H), 2.33 - 2,41 (m, H i). 0.96 - 1,05 (nr. i l l). 0.55 - 0.60 (m, i l l). 0.40 - 0.45 (m, i l l). 0.24 - 0.29 (m, 1H), 0.12 - 0.18 (m, 1H).

E. Ethyl (»S -3-cyclopropyl-3-(3-((diethoxyphosphoryl)methyl)phenyl)propa noate, 35e

To a solution of compound 35d (1 g, 4.27 mmol) in toluene (30 mL) was added diiodozinc (2.5 g, 7.83 mmol) and triethyl phosphite (5 g, 30.09 mmol). The resulting solution was stirred overnight at 120 °C under N ₂ . After cooling to rt, the reaction was quenched with water/ice (100 mL). The resulting mixture was extracted with EtOAc (2 x 300 mL). The organic layers were combined, dried over anhydrous sodium sulfate and concentrated. The resulting residue was purified by flash column chromatography (0-50% EtOAc/DCM) on silica gel to obtain compound 35e as a yellow oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for ¾Η ₂₉ 0 ₅ Ρ· 369.2 (M+H); found: 369.2.

F. Ethyl (»S -3-cyclopropyl-3-(3-(2-(4-(5,5-dimethylcyclopent-l -en-l-yl)-3-(2-fluoro- 5-methoxyphenyl)isoxazol-5-yl)vinyl)phenyl)propanoate, 35f

To a mixture of compound 35e (134 mg, 0.36 mmol) and 15-crown-5 (50 mg) in TITF (2 mL) was added sodium hydride (16 mg, 0.4 mmol, 60% in mineral oil). After stirring at rt for 15 min, compound 35b (100 mg, 0.32 mmol) was added, and the resulting solution was stirred for 2 h at rt. The reaction was then quenched with NH ₄ C1 (satd, 5 mL). The resulting solution was extracted with EtOAc (3 x 5 mL), and the organic layers were combined, washed with H ₂ 0 (2 x 10 mL) and concentrated under reduced pressure. The residue was purified by flash column chromatography (0-5% EtO Ac/petroleum ether) on silica gel to obtain compound 3Sf as a yellow oil. Mass Spectrum (LCMS, ESI pos.): Calcd. for CY.i 1 ^!AC _. 530.3 (M+H); found: 530.2. G. ( ^, ,E)-3-Cyclopropyl-3-(3-(2-(4-(5,5-dimethylcyclopent-l -en-l-yl)-3-(2-fluoro-5- methoxyphenyl)isoxazol-5-yl)vinyl)phenyl)propanoic acid (Cpd 56)

and

(S,Z)-3-cyclopropyl-3-(3-(2-(4"(5,5-dimethylcyclopent-l-en-l -yl)-3-(2-fluoro-5- methoxyphenyl)isoxazol-5-yl)vinyl)phenyl)propanoic acid (Cpd 59)

A mixture of compound 35f (30 mg, 0.056 mmol) and LiOH ^' H ₂ 0 (24 mg, 0.57 mmoi) in THF (4 mL)/water (1 niL)/methanoi (1 mL) was stirred overnight at rt. The reaction mixture was concentrated under reduced pressure. The residue was dissolved in water (5 mL), and the pH of the solution was adjusted to ~5 with HCl (1 N). The precipitates were collected by filtration. The crude product was purified by chirai HPLC with the following conditions: column, repaired ADH, 21.2* 150mm, Sum; mobile phase, hex (0.1% TFA) and IP A (hold 40.0% IPA in 8 min); Detector, UV 254/220 nm to provide compound 56 and compound 59.

Compound 56: ^l H~NMR (300 MHz. D\!SiW< ) δ (ppm): 12.00 (brs, i l l ). 7.44 - 7.54 (m, 21 1 ). 7.37 (s, 1H), 7.25 - 7.32 (m, 3H), 7,04 - 7.12 (m, 2H), 6,90 - 6.95 (m, 1 1 1 ). 5.94 (s, 1H), 3.75 (s, 3H), 2,69 - 2.72 (m, 2H), 2,32 - 2.45 (m, 3H), 1.73 (t, ,/ 6.9 Hz, 2H), 1.03 - 1.06 (m, 1H), 0,70 (s, 6H), 0,52 - 0.53 (m, I I I ). 0,26 - 0.34 (m, 2H), 0.15 - 0.17(m, Mass Spectrum (LCMS, ESI pos.): Calcd, for C ₃₁ H ₃₂ FNO ₄ : 502.2 [M+H] ⁺ ; found: 502.2.

Compound 59: ^I-NMR (300 MHz, DMSO- δ (ppm): 7.24 - 7.38 (m, 5H), 6.93

- 7.1 1 Or, 31 1 ). 6.36 (d, J = 12.6 Hz, IH), 5.82 (s, l i s ). 3.74 (s, 3H), 2.62 - 2.64 (m, 2H), 2.26 - 2,36 (m, 3H), 1 ,70 - 1 .70 (t, ./ 6.9 Hz, 2H), 0.95 - 1.07 (rn, l i s ). 0.70 (s, 61 1 ). 0.52

- 0.53 (m, 1H), 0.26 - 0,34 (m, 2H), 0.15 - 0.17(m, 1 H). Mass Spectrum (LCMS, ESI pos.): Calcd, for C31H32FNO4: 502.2 ! X i l l ) . found: 502.2.

Example 36

(5)-3-Cyclopropyl-3-(3-((4-(5,5-dimethylcyclopent-l -en-l-yl)-3-(2-fluoro-5- methoxyphenyl)isothiazol-5-yl)methoxy)phenyl)propanoic acid (Cpd 61)

A. 2-Fluoro-5-methoxybenzamide, 36a

To a solution of 2-fluoro-5-methoxybenzonitrile (2 g, 13.2 mmoi) in DMSO (6.6 mL) was added l 0 ₂ (1.6 mL) and K ₂ C0 ₃ (274 mg, 1.98 mmoi). The resulting solution was stirred for 30 mm at it. The reaction was then quenched with NaHC0 ₃ (said, 30 mL). The resulting solution was extracted with EtO Ac (50 mL), and the organic layers were combined, and concentrated under reduced pressure. The residue was purified by flash column chromatography (0-10% EtO Ac/petroleum ether) on silica gel to obtain compound 36a as a white solid. ^I-NM (400 MHz, DMSQ-t¾ δ (ppm): 7.66 - 7.70 (m, 2H), 7.04 - 7.23 (m, 3H), 3.77 (s, 3H).

B. 5-(2-Fluoro-5-methoxyphenyl)- 1 ,3,4-oxathiazol-2-one, 36b

A mixture of 36a (2.86 g, 16.9 mmoi) and chloro(chlorosulfanyl)methanone (3.29 g, 25.1 mmol) in toluene (50 mL) was stirred overnight at 100 °C in an oil bath. The resulting mixture was concentrated under reduced pressure. The residue was purified by flash column chromatography (0-20% EtO Ac/petroleum ether) on silica gel to obtain compound 36b as a yellow solid. Ί i-\\!R (300 MHz, DMSO-6¾ δ (ppm): 7.24 - 7.49 (m, 3H), 3.80 (s, 3H).

C. Ethyl 3-(2-fluoro-5-methoxyphenyl)isothiazole-5-carboxylate, 36c

A mixture of compound 36b (2.4 g, 10.6 mmol) and ethyl but-3-ynoate (2.37 g, 21. 1 mmol) in 1 ,3-dichlorobenzene (8 ml.) was stirred for 5 h at 150 °C in an oil bath. After cooling to rt, the reaction was quenched with NH ₄ CI (satd, 10 mL). The resulting solution was extracted with EtO Ac (3 x 15 mL), and the organic layers were combined and concentrated under reduced pressure. The residue was purified by flash column chromatography (0-10% EtO Ac/petroleum ether) on silica gel to obtain compound 36c as a yellow solid. Mass Spectrum (LCMS, ESI pos.): Calcd. for ( ,! 1 ; · Ι· \0 ;8: 282.1 [M+H] ⁺ ; found: 282. 1.

D. (5 3-Cyclopropyl-3-(3-((4-(5,5-dimethylcyclopent-l-en-l-yl)-3-( 2-fluoro-5

methoxyphenyl)isothiazol-5-yl)methoxy)phenyl)propanoic acid, Cpd 61

Compound 61 was prepared from compound 36c following similar procedures as described in Example 7, Steps E-H. 1H-NMR (300 MHz, OMSO-d ₆ ) δ (ppm): 7.83 (d, J ------- 3.0 Hz,

1 H), 7.56 (d, I 6.3 Hz, I H), 7.17 - 7.23 (m, IH), 6.96 - 7.00 (m, 2H), 6.85 - 6,89 (m, 2H), 5.60 - 5,70 (m, IH), 5.55 (s, 2H), 3.78 (s, 3H), 2.28 - 2.39 (m, 5H), 1.79 - 1 .84 (m, 2H), 1.23 (s, 6H), 0.96 - 0.98 (m, I H), 0,40 - 0.50 (m, I H), 0, 1 8 - 0.30 (m, 2H), 0.01 - 0,09 (m, IH). Mass Spectrum (LCMS, ESI pos.): Calcd. for C ₃₀ H ₃₂ FNO ₄ S: 522.2 (M I I); found: 522.0.

Example 37

(5)-3-(3-((5-( i?rt-butyl)-4-(2-fluoro-5-methoxyphenyl)thiophen-2-yl)methox y)phenyl)-3- cyclopropylpropanoic acid (Cpd 60)

A. Methyl 4-bxOxno-5-(tert-butyl)thiophene-2-carboxylate, 37a

A solution of the methyl 5-'(tert iutyl)ihiophene--2--car oxyiate (1.0 g, 5 nimol) in€1¾C1 ₂ (5 mL) was added dropwise to a stirred suspension of A!CU (1,7 g, 12 rnmol) in CI¾(¾ (5 mL) at 0 °C. The reaction mixture was then stirred for an additional 30 mm. A solution of Br ₂ in CH?.C1 ₂ (2 M, 2.5 mL, 5 mmo!) was added at the same temperature and the stirring was continued for 1 h. The reaction mixture was poured onto acidic crushed ice (5 g, cone, aq HCI (2 mL) and extracted with C3¾C! ₂ (2 ^x 10 mL). The combined organic layers were washed successively with brine (10 mL) and satd aq NaHCO? (10 niL) and subsequently dried over MgSOi. The solvent was evaporated under reduced pressure and the residue was distilled to give compound 37a. ^! H- NMR (300 MHz, CDC1 ₃ ) δ (ppm): 7.62 (s, 1H), 5.30 (s, H), 3.86 (s, 3H), 1.51 (s, 9H).

B. Methyl 5-(tert-buiyi)-4-(2-fluoxO-5-meihoxyphenyi)thiophene-2-caf boxy late, 37b

A mixture of compound 37a (459 mg, 1.65 mmol), (2-fluoro-5-methoxyphenyl)boronic acid (338 mg, 1.98 mmol), Pd(PPh ₃ ) ₄ (191 mg, 0.166mmol) and K ₂ C0 ₃ (572 mg, 4.14 mmol) in dioxane (10 ml.) was stirred for 48 h at 98 °C under N ₂ . The reaction mixture was allowed to cool to rt and was treated with water (15 mL). The resulting solution was extracted with EtOAc (3 10 mL). The organic layers were combined, dried over anhydrous sodium sulfate and concentrated. The residue obtained was purified by column chromatography on silica gel (EtO Ac/petroleum ether, 1 : 100-1 :20) to give compound 37b. ^! H -NMR (300 MHz, CDCI ₃ ) δ: 7.47-7.52 (m, lH), 6.97-7.04 (m, 1H), 6.82-6.91 (m, IH), 6.75 (dd, j 6. 1. 3.0 Hz, IH), 3.85 (s, 3H), 3.77 (s, 3H), 1.25-1.33 (m, 9H).

(5-(7ert-bu1yl)-4-(2-fiuoro-5-methoxyphenyl)thiophen-2-yl )methanol, 3 ^'

To a solution of compound 37b (258 mg, 0.800 mmol) in THF, LAH (1 M in THF, 0.800 mL, 1.00 mmol) was added at 0 °C. The resulting mixture was stirred for 30 mm at 0 °C. Water (0.1 mL), 3N NaOH (0.1 mL), and water (0.3 mL) were added sequentially with stirnng. The mixture was extracted with DCM (3 x 20 mL). The combined organic phases were dried and then concentrated to give compound 37c. Mass Spectrum (LCMS, ESI pos.): Calcd. for Ci ₆ H ₁₉ F0 ₂ S: 317.1 (M+Na); found: 317.0.

D. (»S)-Methyl 3-(3-((5-(tert-butyl)-4-(2-fluoro-5-methoxyphenyl)thiophen-2 - yl)methoxy)phenyl -3-cyclopropylpropanoate, 37 d

To a mixture of methyl (3»S)-3-cyclopropyl-3-(3-hydroxyphenyl)propanoate (75 mg, 0.34 mmol), compound 37c (100 mg, 0.34 mmol) and Ph ₃ P (0,26 g, 0.51 mmol) in DCM (10 mL), was added DEAD (80.67 μΕ, 0.51 mmol) dropwise at 0 °C. The resulting mixture was allowed to warm to rt with stirring overnight. The reaction mixture was filtered and concentrated. The residue obtained was purified by flash column chromatography on silica gel (0-50% EtO Ac/heptane) to give compound 37d. Mass Spectrum (LCMS, ESI pos.): Calcd. for C29H33FO4S: 519.6 (M+Na); found: 519.2.

E. ( ₁ S)-3-(3-((5-(tert-butyl)-4-(2-fluoro-5-methoxyphenyl)t hiophen-2- yl)methoxy)phenyl)-3-cyclopropylpropanoic acid, Cpd 6Θ

The title compound 6Θ was prepared from compound 37d following the procedure described in Example 3, Step H. 1H- NMR (300 MHz, CDCi ₃ ) δ: 7. 19-7.27 (m, 1H), 6.95- 7.03 (m, 1H), 6.74-6.91 (m, 6H), 5.10 (s, 2H), 3.73-3.80 (m, 3H), 2,70-2.83 (m, 2H), 2,30- 2.40 (m, 1 1 1 ). 1.22-1.31 (m, 91 1 ). 0.94-1 ,07 (m, H i ). 0.52-0,62 (m, i l l ). 0.36-0.46 (m, i l l ). 0.28 (dq, J=9.5, 4,7 Hz, 1H), 0. 15 (dq, J 9 5. 4.9 Hz, 1H), Mass Spectrum (LCMS, ESI pos.): Calcd, for 505.6 (M+Na); found: 505.1 , Biological Examples

In Vitro Assays

GPR40 Calcium Flux Assay

Compounds were tested in a calcium flux assay using transfected HEK293 cells stably expressing either human GPR40 or rat GPR40. Human GPR40 expressing cells were cultured in DMEM-High Glucose media supplemented with 10% fetal bovine serum, 1 x L-Glutamine, 1 x Penicillin/Streptomycin and 500 }ig/mL G418. Rat GPR40 expressing cells were cultured in DMEM-High Glucose media supplemented with 10% fetal bovine serum and 1 ,ug/niL puromycin. Cells were plated into poly-D-lysine coated 384-well plates and cultured overnight in a 37 °C humidified tissue culture incubator under 5 % CO ₂ /90 % 0 ₂ atmosphere. On the day of the experiment, the culture media was replaced with assay buffer (HBSS, 20 mM HEPES, 0.1 % BSA) and the cells incubated at 37 °C for 1 h. Calcium-sensitive fluorescent dye (Fluo 8 No-Wash Calcium Dye, ABD Bioquest) was then added and the cells incubated for another 30 nun at 37 °C followed by 15 nun at room temperature while protected from the light. The cell plate and a plate of diluted compounds of Formula (I) were loaded into a fluorescent plate reader that added compounds onto the cells while measuring the fluorescence intensity of each well. The plate reader recorded fluorescence intensity at 1 second intervals for 8 min and provided the data for analysis in an Excel format. EC50 values were calculated using Prism

(GraphPad) software. Resultant data are shown in Table 3.

Table 3.

Cpd hGPR40 Ca ^21" Assay rGPR40 Ca ²⁺ Assay EC50 (μΜ) ECSO (μΜ)

4 0.005 0.010

5 0,037, 0.035, 0.006 0.069, 0.046, 0,056

6 0.021 0.129

7 0.024 0.069

8 0.113, 0.103, 0,027 0,044, 0. 144, 0.143

9 0.053 0. 138

10 0.053, 0.035, 0.034 0.091, 0.089

11 0.058 NT

12 1 .266 NT

0.053, 0.051 , 0.022,

13 NT

0.013, 0.087, 0.071

14 0.108, 0,089 NT

0.002, 0.002, 0.002,

0.003, 0.002, 0.005,

15 0.003, 0.001 , 0.002, NT

0.001, 0.004, 0.004,

0.018, 0.015, 0.003,

0.002

16 0,087, 0.065 NT

17 0.242, 0.232 NT

18 0.305, 0.152 NT Cpd hGPR40 Ca ^21" Assay rGPR40 Ca ²⁺ Assay EC50 (μΜ) ECSO (μΜ)

19 0.253, 0.128 NT

20 0.079, 0,071 NT

21 0.110, 0,066 NT

22 0.047, 0,041 NT

23 0,016, 0.015 NT

24 0,068, 0.066 NT

25 0.101 , 0.068 NT

26 0.026, 0,024 NT

27 0.053, 0,042 NT

28 0.083, 0.074 NT

29 0.320, 0.211 NT

30 0.0320, 0.0270 NT

31 0.258, 0.162 NT

32 0.031, 0.027 NT

33 0.215, 0, 178 NT

34 0.168, 0.140 NT

35 0.008, 0.006 NT

36 0,030, 0.026 NT

37 0,065, 0.055 NT Cpd hGPR40 Ca ^21" Assay rGPR40 Ca ²⁺ Assay

EC50 (μΜ) ECSO (μΜ)

38 0.1 15, 0.092 NT

39 0.005, 0,004 NT

40 0.008, 0,007 NT

41 0.014, 0.012 NT

42 0,019, 0.015 NT

43 0.01 1 , 0.009 NT

45 0.023, 0.021 NT

46 0.007, 0,005 NT

47 0.020, 0,016 NT

48 0.024, 0.021 NT

49 0.067, 0.056 NT

50 0.1 10, 0.081 NT

51 0, 180, 0.140 NT

52 0.051, 0.045 NT

* The notation "NT" means Not Tested. Where multiple values are displayed for a single compound, these values are representative of values determined upon repeated testing.

While the foregoing specification teaches the principles of the present invention, with examples provided for the purpose of illustration, it will he understood that the practice of the invention encompasses all of the usual variations, adaptations and/or modifications as come within the scope of the following claims and their equivalents.