Title:
GROWTH OF WILD-TYPE HEPATITIS A VIRUS IN CELL CULTURE
Document Type and Number:
WIPO Patent Application WO2007117258
Kind Code:
A9
Abstract:
The invention provides recombinant Hepatitis A Virus (HAV) nucleic acids and host cells that are permissive for their growth and replication. The recombinant Hepatitis A Virus nucleic acids not particularly limited, except that they incorporate at least one heterologous nucleic acid fragment. The heterologous nucleic acid can encode a selectable marker gene and such recombinant HAV nucleic acids are useful for selecting cells that are permissive for growth and replication of wild type HAV. Alternatively, the heterologous nucleic acid may encode a vaccine antigen or other expression product that is desirable to express in a cell harboring the recombinant HAV nucleic acid. The invention further provides cell lines permissive for growth and replication of wild type HAV or HAV having minimal mutations for growth in cell culture. The invention further provides methods for producing HAV vaccines and for monitoring environmental and patient samples for the presence of HAV.
Inventors:
KAPLAN GERARDO (US)
KONDURU KRISHNAMURTHY (US)
KONDURU KRISHNAMURTHY (US)
Application Number:
PCT/US2006/024748
Publication Date:
April 17, 2008
Filing Date:
June 26, 2006
Export Citation:
Assignee:
US GOV HEALTH & HUMAN SERV (US)
KAPLAN GERARDO (US)
KONDURU KRISHNAMURTHY (US)
KAPLAN GERARDO (US)
KONDURU KRISHNAMURTHY (US)
International Classes:
C12N15/86; C12N5/00
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