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Title:
HEALTH PROTECTIVE HERBAL SOFT DRINK
Document Type and Number:
WIPO Patent Application WO/2004/056212
Kind Code:
A1
Abstract:
The invention provides a novel herbal soft drink comprising decoction of plants selected from Phyllanthus sps., Glycyrrhiza glabra, Vitis vinifera, Withania somnifera, Boerhaavia diffusa, Tinospora cordifolia, Elettaria cardamomum and Cinnomomum sps. for the protection of liver disorders in particular along with more antioxidant, immunoenhancing, gastrointestinal compatibility, diuretic, nervine relaxant and cardio-tonic properties and a process for preparing the same.

Inventors:
PALPU PUSHPANGADAN (IN)
SHANTA MEHROTRA (IN)
SINGH RAWAT AJAY KUMAR (IN)
SAYYADA KHATOON (IN)
KUMAR OJHA SANJEEV (IN)
SUBHA RASTOGI (IN)
MANJOOSHA SRIVASTAVA (IN)
KISHORE SETH PRAHLAD (IN)
KUMAR AGARWAL ASHOK (IN)
POONAM KAKKAR (IN)
MOHINI ANAND (IN)
Application Number:
PCT/IB2002/005555
Publication Date:
July 08, 2004
Filing Date:
December 20, 2002
Export Citation:
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Assignee:
COUNCIL SCIENT IND RES (IN)
PALPU PUSHPANGADAN (IN)
SHANTA MEHROTRA (IN)
SINGH RAWAT AJAY KUMAR (IN)
SAYYADA KHATOON (IN)
KUMAR OJHA SANJEEV (IN)
SUBHA RASTOGI (IN)
MANJOOSHA SRIVASTAVA (IN)
KISHORE SETH PRAHLAD (IN)
KUMAR AGARWAL ASHOK (IN)
POONAM KAKKAR (IN)
MOHINI ANAND (IN)
International Classes:
A23L1/30; A23L2/02; A23L2/38; A61K36/185; A61K36/47; A61K36/48; A61K36/54; A61K36/59; A61K36/81; A61K36/87; A61K36/9064; (IPC1-7): A23L2/02; A23L2/38; A23L2/54; A61K35/78
Domestic Patent References:
WO1998005346A11998-02-12
Foreign References:
US20020025349A12002-02-28
CN1251278A2000-04-26
CN1208586A1999-02-24
GB981487A1965-01-27
Other References:
"AMRITARISHTA, ASHWAGANDHARISHTA, DRAKSHAVA, DRAHARISHTA, PUNARNAVARISHTA", XP002247468, Retrieved from the Internet [retrieved on 20030617]
DATABASE WPI Section Ch Week 198716, Derwent World Patents Index; Class D16, AN 1987-114519, XP002247469
Attorney, Agent or Firm:
Gopalan, Deepak Sriniwas (84-C C6 Lane, Off Central Avenu, Sainik Farms 2 New Delhi, IN)
Download PDF:
Claims:
Claims:
1. A herbal soft drink comprising of : a concentrated herbal extract obtained from a mixture of herbs selected from Phyllanthus sps., Glycyrrhiza glabra, Boerhaavia diffusa, Vitis vinifera, Tinospora cordifolia and Withania somnifera along with jaggery, Elettaria cardamomum, Cinnamonum sps., a fermenting agent and carbonated water.
2. A herbal soft drink as claimed in claim 1, wherein the percentage ratio of Phyllanthus sps. : Glycyrrhiza glabra : Boerhaavia diffusa : Vitis vinifera : Tinospora cordifolia : Withania somnifera used for preparing the extract is in the range of 5 to 10: 5 to 10 : 5 to 10 : 15 to 20 : 5 to 10 : 5 to 10.
3. A herbal soft drink as claimed in claim 1, wherein the w/w ratio of the jaggery : concentrated herbal extract is in the range of 1: 3 to 1: 4.
4. A herbal soft drink as claimed in claim 1, wherein the w/w ratio of Elettaria cardamomum : Cinnamomum sps. used is in the range of 1: 1 to 2: 1.
5. A herbal soft drink as claimed in claim 1, wherein the fermenting agent used is Sacromyces strain and flowers of Woodfordia fructose.
6. A herbal soft drink as claimed in claim 5, wherein the percentage ratio of fermenting agent added is in the range of 4 to 16.
7. A herbal soft drink as claimed in claim 1, wherein the w/w ratio of carbonated water: the mixture of the concentrated extract, jaggery, Elettaria cardamomum, Cinnamomum sps. and the fermenting agent is in the range of 1: 3 to 1: 5.
8. A herbal soft drink as claimed in claim 1, wherein the soft drink provides antioxidant, hepatoprotective, cardiotonic, diuretic, digestive, choleretic, nervine relaxant and immunoenhancing properties.
9. A herbal soft drink as claimed in claim 1, wherein total solids content in the soft drink ranges from 3040%.
10. A process for preparing the herbal soft drink having antioxidant, hepatoprotective, cardiotonic, diuretic, digestive, choleretic, nervine relaxant and immunoenhancing properties, the said process comprising the steps of : (a) obtaining plant parts of Phyllanthus sps., Glycyrrhiza glabra, Boerhaavia diffusa, Vitis vinifera, Tinospora cordifolia and Withania somnifera ; (b) crushing the plant parts and mixing them to obtain a powdered mixture; (c) adding water to the powdered mixture of step (b) to obtain an aqueous extract; (d) concentrating the aqueous extract of step (c); (e) filtering the concentrated extract of step (d); (f) mixing jaggery to the filtered extract of step (e); (g) adding Sacromyces strain and a fermenting agents to the mixture of step (f); (h) further adding Elettaria cardamomum and Cinnamonaum sps. in the w/w ratio ranging from 1: 1: 30 to 1: 1: 50 to the mixture of step (g); (i) fermenting the mixture of step (h) for a time period ranging between 3 to 6 days; (j) filtering the fermented mixture of step (i); (k) concentrating the fermented filtrate of step (j) to obtain a stock solution, and (1) mixing the stock solution of step (k) with carbonated water in the w/w ratio of 1: 3 to 1: 5 to obtain the herbal soft drink.
11. A process as claimed in claim 10, wherein the plant parts used are selected from the group consisting of leaf, stem, root, fruits and whole plant.
12. A process as claimed in claim 10 wherein in step (b), the percentage ratio of Phyllanthus sps. : Glycyrrhiza glabra : Boerhaavia diffusa : Vitis vinifera : Tinospora cordifolia : Withania somnifera in the powdered mixture is in the range of 5 to 10: 5 to 10: 5 to 10: 15 to 20: 5 to 10: 5 to 10.
13. A process as claimed in claim 10 wherein in step (c), the w/w ratio of water added to the powdered mixture is in the range of 5 : 1 to 10 : 1.
14. A process as claimed in claim 10 wherein in step (d), the aqueous extract is concentrated to 1/3 to 1/4 of its original volume.
15. A process as claimed in claim 10 wherein in step (f), the w/w ratio of the jaggery: filtered extract is in the range of 1: 3 to 1: 4.
16. A process as claimed in claim 10 wherein in step (g), the fermenting agent used is selected from Sacromyces strain and flowers of Woodfordia fructose.
17. A process as claimed in claim 10 wherein in step (h), the w/w ratio of Elettaria cardamomum : Cin7zamomum sps. used is in the range of 1: 1 to 2: 1.
18. A process as claimed in claim 10 wherein in step (k), the fermented filtrate is concentrated to 4/5 to 1/5 of its original volume.
Description:
HEALTH PROTECTIVE HERBAL SOFT DRINK TECHNICAL FIELD The present invention relates to the development of health protective herbal soft drink.

BACKGROUND ART Diet or food therapy is emerging as the latest trend in health care program. It is now said that there will be more dieticians than physician in the present century as many diseases can be prevented if the right kind of diet is prescribed. Herbs into our lives in an ongoing way, it is easier to modify an existing habit than to create a new one. Consumption of the right kind of food articles and drinks suited to the climate, age and constitutional, natures of the individuals are getting greater scientific security and attention. The rich and diverse traditional diet practices prevalent among various communities with the regional variations are now found to health protective/promoting. Over 1000 different kinds of alcoholic drinks, soft drinks, beverages and medicinal drinks are traditionally consumed in India. But unfortunately with the introduction of various exotic drinks many of the local drinks which are mostly plant based are fast disappearing and some of them almost forgotten.

Acknowledging this truth. The inventors undertook a critical study of many of such drink and with the modern scientific understanding of beverages have designed herbal soft drinlc.

This drink is fortified with many health protective and promotive attributes such as hepatoprotective, anti-oxidant and immuno-enhancing, besides providing instant energy and vitality. These properties have been authenticated/validated through appropriate pharmacological investigations and clinical studies. The drink is diuretic and has cooling effect like other soft drinks of summer. The result will be a boon to consumers, in terms of herbal alternatives and availability.

Reference is made to website http://www. holistichealthplus. com/HT/hepatitis. htm wherein herbs with powerful liver protective properties, adding in detoxification and promoting bile production and flow, as well as nourishing and repairing liver tissue includes. Phyllantlzus amarus and Glycyrrhiza glabra.

Reference is made to web site http ://www. drlindaberry. comlproductshnetagenics/mics. htm wherein herbal formulation Liv. 52 for liver disorders contains Phyllanthus fziruril P. amarus, Boerlzaavia diffusa and Tinospora cordifolia along with some other medicinal plants.

The reference is made to Charak Samhita wherein Glycyrrhiza glabra is used for the activity of-Jeevaneeya, Sandhaneeya, Varnya, Kanthaya, Kandughra, Sanehopaga, Vamanopaga, Asthapanopaga, Aizgamard parashamanam, Shozzitsthapana and

Mutravirarzjaneeya i. e. Nutrient, Union promoters, complex promoters, Voice promoters, Anti-prurities. Adjuvant to oleation therapy, Adjuvant in Emetic therapy, Adjuvant in non oily enemata, Restoratives, Haemostatic and corrective of urinary pigments. Further it is good for eyes, hairs, voice and lusture and aphrodisiac.

Reference is made to Bhaishajya Ratnavali l Hikkaswasschiktsa for"Glycyrz°lzizia glabra" as one the ingredient of"Kanakasava"with twelve other ingredients including jaggery as base for fermentation, Vitis vinifiera, and Woodfordia fruticosa, indicated in chronic bronchitis, asthma and acts as expectorant.

Reference is made to website http ://www. greencanyon. com/products/clO0149. htm. wherein Licorice root is used in tea and capsules for the treatment of bronchitis, digestive tract spasm, cough, stomach, inflammation and as a tonic for the adrenal.

Reference is made to website http ://www. wizardofevez. com/uterine. fibroid. html wherein Glycyrrl ? iza glabra along with some other ingredients used as hormonal tonic to correct hyperestrinism: from end of menses to ovulation.

Reference is made to website http : //www. healthandage. com/html/res/COM/cousconditions /obteomvelitisec. html wherein infusion of Glycyrrhiza glabra along with some other herbs mentioned as alternative herbs as blood cleanser and also mentioned that do not use Glycyrrhiza glabra the hypertensive persons.

Reference is made to website http://www. globalnutrients. net/herbal_powders_ information. htm root of Glycyrrhiza glabra raw or used as flavoring. The source of licorice powder that is extracted and used in sweets (candies), baked goods, ice cream, soft drinks etc, it is also used medicinally. The root contains glycyrrhizin, a substance that is 50 times sweeter than sources. The dried root is often used for chewing, it is excellent for teething children and also as a tooth cleaner. A tea made from the roots is an excellent thirst quencher. The powdered root is also used as a sweetener in other herbs teas. The leaves are used as a tea substitute in Mongolia. Extracts from the root are used as a foaming agent in beers and fire extinguishers.

Reference made to US patent 6,416, 807 wherein mixed fine powder for beverage containing licorice as one of its ingredients.

Reference made to US patent 6,280, 776 wherein a composition; food eupplements, healthcare foods comparing water extracts of Panax pscudo-ginseng, Eucommia ulmoides, gallic acid and licorice root for improvement of nutritional state, hypertension, high blood sugar, immune system and liver disorders.

Reference is made to US patent 6,210, 738 wherein freeze dried ginseng berry tea which comprises one or more natural health promoting ingredients selected from a group of plants which also include. Elettaria cardamomum, Cinnamomum verum, Glycyrrhiza glabra and Withania somnifera.

Reference is made to US patent 6,187, 313 wherein a composition and method for treating and preventing helicobactor-phylariassociated stomach gastritis, ulcers and cancer comprising degly cyrrhizinated licorice extract.

Reference is made to classical Ayurvedic text of"Laghutrayi"-Sharaagdhar Samhital Madhyam Khandl Chapt-10 for"Vitis vinifera", which is used as major ingredient of "Draksharishta" with ten minor ingredients including jaggery as base for fermentation, Elettaria cardamomum, Cinnamomum and Woodfordia fruticosa, indicated for "Urahkshat"i. e. phthisis (an advance stage of pulmonary tuberculosis) Reference is made to Yoga Ratnakar/Grahani Chikitsa for"Vitis vinifera", which is used as major ingredient of"Dralcshasava"with sixteen other ingredients including jaggery as base for fermentation, Elettaria cardamomum, Cinnamonum and Woodfordia fruticosa, recommended for alleviating conditions of emaciation and improving digestive and respiratory functions, used as a tonic for vigour and vitality.

Reference is made to US patent 6375992 wherein a composition for hydrating mammalian skin comprises red grape extract.

Reference is made to classical text of Ayurvedic formulation-. Ratanavalil Murchcha Rogadhikar for"Withania somnifera", which is major ingredient of "Aswagandharishta"with twenty-eight other ingredients including jaggery as base for fermentation, Elettaria cardamomum, Cinnamomum, Glycyrrhiza glabra and Woodfordia fruticosa, and indicated for general debility and weakness, relieving tension and anxiety.

Reference is made of website http://www. globalnutrients. net/herbal_powders_ information. htm wherein Withania somnifera is one of the most widespread tranquilizes used in India, where it hold a position of importance similar to ginseng in China. It acts mainly on the reproductive and nervous systems, having a rejuvenative effect on the body, and is used to improve vitality and aid recovery after chronic illness.

Reference is made to US Patent 6,153, 198 wherein an alcoholic extract Withania somnifera produces a cognition effect and learning facility for the user.

Reference is made to US patent 5,494, 668 wherein a composition for treating musculoskeletal disease such as rheumatoid arthritis and osteoarthritis comprises of extracts of Aswagandha-withania somnifera along with some other plant material extracts.

Reference is made to Bhaishjya Ratnavali/Slaotla Rogadhika for"Boerhaavia diffusa" used as major ingredient of"Punarnavarishta"with seventeen other ingredients including jaggery as base for fermentation, Elettaria cardamomum, Cinnamomum, Sida cordifolia, Tinospora cordifolia and Woodfordia fruticosa, used for diuretic activity indicated in the treatment of oedema and ascites.

Reference is made to Bhaishjya Ratnavali/Jwaradlzikar for"Tinospora cordifolia"used as major ingredient of"Anznitarishta"with thirteen other ingredients including jaggery as base for fermentation, Elettaria cardamomum, Cinnamonum and Woodfordia fruticosa, indicated for treatment of fever and peripheral neuritis.

Reference is made to website http://www. herbpatch. com/herbs&7. htm wherein Tinospora cordifolia along with some other medicinal plants used in a formulation an Ayurvedic formulation SKN-AV, to restore the body to balanced state will being, the skin in particular.

Reference is made to US 5886029 wherein a medicinal composition for treatment of diabetes comprises Tinospora cordifolia and cinnamomum tamala along with some other ingredients.

Reference is made to US patent 6,136, 316 wherein a hepatoprotetive composition for treating acute hepatitis B and E virus infection comprises Phyllanthu. s amarus, Tinospora cordifoia and Boerhavia diffusa.

Reference is made to US Patent 5693327 for the treatment of skin disorders at least one wherein a herbal composition for the treatment of skin disorders comprises of at least one plant from Tinospora cordifolia, Glycyrrhiza glabra and Playllanthus emblica along with some other listed plants An Ayurvedic composition for the prophylaxis and treatment of AIDS, flu, TB, hepatitis and other immuno-deficiancies comprises of Tinospora cordifolia, Phyllanthus nirurii, phyllanthus emblica along with some other plant ingredients.

Reference is made to US patent 5683698 wherein a formulation for alleviating symptoms associated with arthritis comprises of Tinospora cordifolia and Withania somnifera along with some other ingredients.

OBJECTS OF THE INVENTION The primary objective of the invention is to provide a soft drink for the protection of liver disorder in particular with more antioxidant, immunoenhancing hepatoprotective, gastro- intestinal compatibility, diuretic, nervine relaxant and cardiotonic properties.

Another objective of the invention is that the drink gives instant energy and combat fatigue.

Yet another objective of the present invention is to produce the health drink with some commonly available Indian medicinal herb.

Still another objective of the invention is that the herbal drink possesses all the advantages of soft drink and none of its disadvantages.

Another objective of the present invention is the development of jaggery based herbal soft drink with non-toxic effect.

SUMMARY OF THE INVENTION The invention provides soft drink comprising decoction of plants selected from Phyllanthus sps., Glycyrrhiza glabra, Vitis vinifera, Withania somnifera, Boerhaavia diffusa, Tinospora cordifolia, Elettaria cardamomunm and Cinno7710mum sps. for the protection of liver disorder in particular with more antioxidant, immunoenhancing hepatoprotective, gastro-intestinal compatibility, diuretic, nervine relaxant and cardiotonic properties.

DETAILED DESCRIPTION OF THE INVENTION The present invention provides a herbal soft drink comprising of : a concentrated herbal extract obtained from a mixture of herbs selected from Phyllanth. us sps., Glycyrrhiza <BR> <BR> <BR> <BR> glabra, Boerhaavia diffusa, Vitis vinifera, Tinospora cordifolia and Withania somnifera along with jaggery, Elettaria cardamomum, Cinnamomum sps., a fermenting agent and carbonated water.

In an embodiment of the present invention, the percentage ratio of Phyllanthus sps. : Glycyrrhiza glabra : Boerhaavia diffusa : Vitis vinifera : Tinospora cordifolia : Withania somnifera used for preparing the extract is in the range of 5 to 10: 5 to 10: 5 to 10: 15 to 20: 5 to 10: 5 to 10.

In another embodiment of the present invention, the w/w ratio of the jaggery: concentrated herbal extract is in the range of 1: 3 to 1: 4.

In yet another embodiment of the present invention, the w/w ratio of Elettaria cardamomum : Cinnamomum sps. used is in the range of 1: 1 to 2: 1.

In still another embodiment of the present invention, the fermenting agent used is Sacromyces strain and flowers of Woodfordia fructose.

In one more embodiment of the present invention, the percentage ratio of fermenting agent added is in the range of 4 to 16.

In one another embodiment of the present invention, the w/w ratio of carbonated water: the mixture of the concentrated extract, jaggery, Elettaria cardamomum, Cinnamomum sps. and the fermenting agent is in the range of 1: 3 to 1: 5.

In another embodiment of the present invention, the soft drink provides antioxidant, hepatoprotective, cardio-tonic, diuretic, digestive, choleretic, nervine relaxant and immune-enhancing properties.

In a further embodiment of the present invention, total solids content in the soft drink ranges from 30-40%.

The present invention also provides a process for preparing the herbal soft drink having antioxidant, hepatoprotective, cardio-tonic, diuretic, digestive, choleretic, nervine relaxant and immuno-enhancing properties, the said process comprising the steps of : (a) obtaining plant parts of Phyllanthus sps., Glycyrrhiza glabra, Boerhaavia diffusa, Vitis vinifera, Tinospora cordifolia and Withania somnifera ; (b) crushing the plant parts and mixing them to obtain a powdered mixture; (c) adding water to the powdered mixture of step (b) to obtain an aqueous extract; (d) concentrating the aqueous extract of step (c); (e) filtering the concentrated extract of step (d); (f) mixing jaggery to the filtered extract of step (e); (g) adding Sacromyces strain and a fermenting agents to the mixture of step (f); (h) further adding Elettaria cardamomum and Cinnarnomurn sps. in the w/w ratio ranging from 1: 1: 30 to 1: 1: 50 to the mixture of step (g); (i) fermenting the mixture of step (h) for a time period ranging between 3 to 6 days; (j) filtering the fermented mixture of step (i); (lu) concentrating the fermented filtrate of step (j) to obtain a stock solution, and (1) mixing the stock solution of step (k) with carbonated water in the w/w ratio of 1: 3 to 1: 5 to obtain the herbal soft drink.

In an embodiment of the present invention, the plant parts used are selected from the group consisting of leaf, stem, root, fruits and whole plant.

In another embodiment of the present invention wherein in step (b), the percentage ratio of Phyllanthus sps. : Glycyrrhiza glabra : Boerhaavia diffusa : Vitis vinifera : Tinospora cordifolia : Withania somnifera in the powdered mixture is in the range of 5 to 10: 5 to 10: 5 to 10 : 15 to 20 : 5 to 10 : 5 to 10.

In yet another embodiment of the present invention wherein in step (c), the w/w ratio of water added to the powdered mixture is in the range of 5 : 1 to 10: 1.

In still another embodiment of the present invention wherein in step (d), the aqueous extract is concentrated to 1/3 to 1/4 of its original volume.

In one more embodiment of the present invention wherein in step (f), the w/w ratio of the jaggery: filtered extract is in the range of 1: 3 to 1: 4.

In one another embodiment of the present invention wherein in step (g), the fermenting agent used is selected from Sacromyces strain and flowers of Woodfordiafructose.

In an embodiment of the present invention wherein in step (h), the w/w ratio of Elettaria cardamomum : Cinnamomum sps. used is in the range of 1: 1 to 2: 1.

In a further embodiment of the present invention wherein in step (k), the fermented filtrate is concentrated to 4/5 to 1/5 of its original volume.

In another embodiment of the present invention, there is provided a herbal soft drink for quenching thirst and cooling effect.

In yet another embodiment of the present invention there is provided herbal soft drink the composition further comprises Phyllanthus sps. Glycyrrhiza glabra for hepatoprotective activity.

In still another embodiment of the present invention, there is provided herbal soft drink the composition comprises Glycyrrlziza glabra for improving gastro-intestinal conditions under stress.

In a further embodiment of the present invention, there is provided herbal soft drink the composition is comprises Elettaria and Cinnamonum sps. to enhance the antioxidant activity.

In one more embodiment of the present invention, there is provided herbal soft drink the composition is comprises Cinnamomum sps. to improve aroma.

In another embodiment of the present invention, plants used have anti-oxidant property.

In yet another embodiment of the present invention, plants used have hepatoprotective property.

In still another embodiment of the present invention, plants used have anxiolytic property.

In another embodiment of the present invention, plants used have no cardiovascular toxicity.

In still another embodiment of the present invention, plants used have diuretic property.

In still another embodiment of the present invention, plants used are non-toxic.

BRIEF DESCRIPTION OF THE TABLES Table 1: provides the SOD activity in brain of animals treated with herbal preparations Cl and C2 (n=5).

Table2: Rate of TBARS formation in brain of rats treated with herbal praperations Cl and C2 (n=5).

Table 3: Level of TBARS formation after invitro supplementation of C1 and C2 to rat brain homopgenate.

Table 4: SOD mimetic activity in vitro in the herbal preparations C1 and C2.

Table 5: The table illustrates some of the properties found to be associated with the plant used in the present invention.

EXAMPLE-1 Phyllanthus sps. - 10% Glycyrrlaiza glabra 10% Boerhaavia diffusa - 5% Vitis vinifera - 15% Tinospora cordifolia 5% Withania somnifera - 5% Elettaria cardamomum - 1% Cinnamomum spp.-1% Woodfordiafruticosa-5% Jaggery - 30% Water-q. s. to make 100 ml decoction EXAMPLE-2 Phyllanthus sps. - 8% Glycyrrhiza glabra 10% Boerhaavia diffusa - 5% Vitis vinifera-15% Tinospora cordifolia - 6% Withania sominifera - 6% Elettaria cardamomum - 1% Cinnamonum spp. 1% Woodfordia fruticosa - 6% Jaggery-30% Water-q. s. to make 100 ml decoction EXAMPLE-3 Phyllanthus sps. - 10% Glycyrrhiza glabra - 8% Boerhaavia diffusa - 5% Vitis vinifera - 18% Tinospora cordifolia - 5% Withania somnifera 6% Elettaria cardamomum - 1. 5% Cinnamomum spp.-1. 5% Woodfordia fruticosa - 5% Jaggery 40% Water q. s. to make 100 ml decoction

EXAMPLE-4 Phyllanthus sps. 8% G glabra - 8% Boerhaavia diffusa - 10% Vitis vinifera - 20% Tinospora cordifolia - 10% Withania somnifera - 10% Elettaria cardamomum - 2% Cinnamomum spp. - 1% Woodfordia fruticosa - 5% Jaggery - 30% Water-q. s. to make 100 ml decoction EXAMPLE-5 Phyllanthus sps. - 5% Glycyrrhiza glabra - 5% Boerhaavia diffusa 5% Vitis vinifera - 20% Tinospora cordifolia - 5% Withania somnifera - 5% Elettaria cardamomum - 1% Cinnamonum spp. - 1% Woodfordia fruticosa - 5% Jaggery-40% Water-q. s. to make 100 ml decoction EXAMPLE-6 Phyllanthus sps. - 7% Glycyrrhiza glabra-5% Boerhaavia diffusa - 8% Vitis vinifera - 15% Tinospora cordifolia-8% Withania somnifera - 8% Elettaria cardamomum - 1.5% Cinnamomum spp. - 1.5% Woodfordia fruticosa - 5% Jaggery-35% Water-q. s. to make 100 ml decoction

EXAMPLE-7 Phyllanthus sps. - 10% Glycyrrhiza glabra - 5% Boerhaavia diffusa-10% Vitis vinifera - 20% Tinospora cordifolia-5% Withania somnifera 5% Elettaria cardamomum - 1% Cinnamomum spp. - 1% Woodfordia fruticosa - 5% Jaggery-35% Water-q. s. to make 100 ml decoction EXAMPLE-8 Phyllanthus sps. - 10% Glycyrrhiza glabra - 10% Boerhaavia diffusa - 8% Vitis vinifera 20% Tinospora cordifolia-8% Withania somnifera 8% Elettaria cardamomum - 1. 5% Cinnamomum spp.-1. 5% Woodfordia fruticosa - 5% Jaggery - 30% Water-q. s. to make 100 ml decoction

Table 1: SOD activity in brain of animals treated with herbal preparations Cl and C2 (n=5). Type of Treatment group Parameter studied treatment SOD activity Protein mg/ml Specific activity units/ml Units/mg protien 7 days oral Control 8. 460. 67 3. 390. 26 2. 490. 23 treatment C2 5.87~0. 39 3. 660. 35 1. 520. 12 30 days oral Control 9. 520. 95 3. 560. 33 2. 650. 59 treatment C2 6.31~0. 51 4. 830. 53 1.30~16 Table2 : Rate of TBARS formation in brain of rats treated with herbal praperations Cl and C2 (n=5) Type of Treatment Parameter studied treatment group nm MDA/mg nm MDA/mg Rate of MDA protein at 0 protein at 60 formation nM minutes minutes MDA/ mg protein/hr 7 days oral Control 1. 350. 46 2. 500. 55 1. 260. 14 treatment C2 0.98~0. 09 1. 900. 25 1. 01~0. 11 30 days oral Control 2. 081. 18 2. 431. 19 0. 4350. 11 treatment C2 1.15~0.04 1.44~0.06 0.313~0.10 Table 3: Level of TBARS formation after irzvitro supplementation of C1 and C2 to rat brain homopgenate

S. NO Supplementation to nM MDA/mg nM MDA/mg protein Rate of MDA formation basal system. protein at 0 minutes at 60 minutes 1. Control 2 1. 001 2. 298 1. 297 2. 501l1 C2 1. 116 1. 106 Nil 3. 100ßl C2 1. 297 1. 221 Nil Table 4: SOD mimetic activity in vitro in the herbal preparations Cl and C2 S. No Sam le SOD mimetic activity units/ml 21. 80

Conclusion Herbal preparation showed SOD Mimetic activity in vitro and radical quenching capacity as can be seen from no change in MDA levels in the presence of this preparation. III vitro also this preparation was found to have antioxidant potential.

Note: SOD Superoxide dismutase TBARS Thiobarbituric acid reactive substances MDA Malondialdehyde Table 5: The table illustrates some of the properties found to be associated with the plant used in the present invention S. No. Plant name Family Chemical Properties (Vernacular constituents name) 1. Phyllanthus spp. Euphorbiaceae Phyllanthin, Astringent, deobstruent, stomachic, (Bhumi amla) hypophyllanthin, diuretic, antifungal, antiseptic, saponin beneficial in jaundice, galactagogue, 2. Glycyrrhiza Leguminosae Glycyrrhizin, Tonic, expectorant, demulcent and glabra asparagines, starch, mildly laxative, used for allaying (Mulethi) mannite, coughs and catarrhal affections, in anethoxanthein, irritability conditions of the mucous glycoside. membrane of urinary organs, heals gastric ulcers, is spasmolytic and stimulates hydrochloric acid secreation. 3. Boerhaavia Nyctaginaceae Quinolizidine Diuretic, anti-inflammatory, for diJ0usa alkaloids, hreahnent of inflammatory renal (Punarnava) punarnavine 1&2, diseases like nephrotic syndrome, fatty oil, potassium oedema and ascites resulting from salts, hypoxanthine-cirrhosis of the liver and chronic 9-L-peritonitis, efficacious in abdominal arabinofuranoside, tumors and cancer, antibacterial, oxalates, myricyl cardiotonic. alcohol, myristic acid, D-glucose, a polysaccharide and punarnavoside, Vitis vinifera Vitaceae Leucoanthocyanins, Stomachic, diuretic, demulcent and (Draksha) sugars, acids and cooling, laxative, and expectorant. phenolics, iron, bromide, iodide and fluoride, vitamins, carotene, thiamine, riboflavin, niacin, vitamin C, pyridoxine, pantothenic acid, folic acid, biotin, inositol, bioflavinoids, carbohydrate, amino acids. tartaric andmalic acids. 5. Tinospora Menispermaceae Glucoside, alkaloidal Antiperiodic, antispasmodic, anti- cordifolia constituents (including inflammatory and antipyretic, for gout, (Giloe) berberine), bitter as liniment in erysipelas, ulcers, as glucoside giloin, tonic, in the treatment of jaundice, giloinin, gilo-sterol, rheumatism and leprosy, has favorable tinosporon, tinosporic effect on endogenous insulin secretion, acid and tinosporol, glucose uptake and inhibition of starch, calcium, peripheral glucose release, phosphorus. 6. Withania Solanaceae Maltose, steroidal Hypotensive, bradycardiac, respiratory- sonznifer-a lactones-withanolides, stimulating action, relaxant and (Aswagandha) withaferin A, antispasmodic effects against several withanone, spasmogens on intestinal, uterine, cuscohygrine, bronchial, treacheal and blood-vascular analygrine, tropine, muscles. inflammatory conditions, pseudotropine, ulcers and scabies. anaferine, isopelletierine, 3- tropyltigloate, nicotine, withasomine, visamine, 7. Elettaria Zingiberaceae Cineol, terpineol, Carminative, aromatic stimulant, eardarzzornum terpinene, limonene, flavouring agent, useful in atonic (Ela) sabinene and terpineol dyspepsia. in the form of formic and acetic esters, borneol, calcium, phosphorus, iron, 8. Ciranarnonauna Lauraceae Eugenol Carminative, anticolic, anti-diarrhoeal, spp. cinnamaldehyde, cinnam anti-rheumatic, anti-cough, anti cold, (Patra/Twak) alacetate, 1, 8-cineol ß-hypoglycaemic, anti-oxidant, caryophyllene, a-antiulcerogenic, aromatic, astringent, humulene, terpeneol, stimulant and expectorant, checks cuminaldehyde, O-nausea and vomiting. methyl eugenol, benzaldehyde, 1-a- pinene, 1-a-and l-ß- phellandrene, p- cymene, caryophyllene, benzyl benzoate and linalool.

ADVANTAGES OF THE PRESENT INVENTION 1. Soft drink is purely herbal.

2. Soft drink for the protection of liver disorders in particular along with more antioxidant, immunoenhancing, gastrointestinal compatibility, diuretic, nervine relaxant and cardio-tonic properties.

3. Soft drink gives instant energy and combat fatigue.

4. The soft drink comprising two or more plants decoction selected from Glycyrrhiza <BR> <BR> glabra, Vitis vinifera, Withania somnifera, Boerhaavia diffusa, Tinospora cordifolia,<BR> <BR> Phyllanthus amarus, Elettaria cardanzomum, Ci. nnomomum sps.

5. Soft drink developed is non-toxic.

6. No chemical preservative or colourants are used in this soft drink.

7. Soft drink is a good source of iron.